2016 Fiscal Year Research-status Report
Identifying the Role of Cell Polarity in 'Real-Time' during Epithelial Homeostasis
| Project/Area Number |
16K21272
|
|---|
| Research Institution | Yokohama City University |
|---|
Principal Investigator |
Goulas Spyros 横浜市立大学, 医学研究科, 特任助教 (90644352)
|
|---|
| Project Period (FY) |
2016-04-01 – 2018-03-31
|
| Keywords | Epithelial Homeostasis / Overproliferation / Cell Death / Barrier Malfunction |
|---|
| Outline of Annual Research Achievements |
Throughout the lifespan of an organism, there is constant cellular turnover, known as homeostasis. When perturbed, this can lead to overproliferation or premature cell death, causing diseases such as cancer. Epithelia have some of the highest levels of cellular turnover and are also highly susceptible to cancers. Loss of polarity has been associated with cancer, but how remains unclear. To understand how epithelia maintain homeostasis, in FY2016 I have screened knockdown lines using a candidate approach of known cell polarity regulators in MDCK cells. I have identified and verified several candidates that resulted in phenotypes during epithelial homeostasis. Some of the phenotypes observed included overproliferation, excessive cell death and epithelial barrier malfunction. These candidates are now being further characterized.
|
| Current Status of Research Progress |
Current Status of Research Progress
3: Progress in research has been slightly delayed.
Reason
Initially, due to technical difficulties of establishing a live automated imaging-based screening system, some delays occurred. These issues could not be resolved so I opted for a ‘non-live’ alternative strategy using stable knockdown to analyze defects in epithelial homeostasis. This circumvented many of the issues and now things are running smoothly.
|
| Strategy for Future Research Activity |
In FY2017, I plan to continue with the proposed project with some alterations. Based on the distinct phenotypes observed, it is possible to categorize them into groups and focus on identifying the potential downstream molecular targets. Furthermore, to establish whether targets identified in vitro have a physiological function in vivo, I will be using the developing Drosophila adult dorsal epithelium (notum).
|
| Causes of Carryover |
In the next fiscal year FY2017, I plan to buy/make antibodies, including Drosophila specific ones, which was originally planned for FY2016. Furthermore, as I was unable to promote my research at an international conference in FY2016, I will do this in FY2017. In FY2017, I also plan for travel visits to my research collaborators at Akita University.
|
| Expenditure Plan for Carryover Budget |
671,440 円 for Article Costs. 400,000 円 to attend an International Research Conference to present the current research. 400,000 円 for Travel Costs to visit collaborators.
|
-
[Journal Article] Oral ingestion of Collagen Hydrolysate Leads to the Transportation of Highly Concentrated Gly-Pro-Hyp and Its Hydrolyzed Form of Pro-Hyp into the Bloodstream and Skin2017
Author(s)
Yazaki, M., Ito, Y., Yamada, M., Goulas, S., Teramoto, S., Nakaya, M., Ohno, S. & Yamaguchi, K.
-
Journal Title
Journal of Agricultural and Food Chemistry
Volume: 65
Pages: 2315-2322
DOI
Peer Reviewed / Open Access / Int'l Joint Research
