Neuro-regenerative effects of MSC and neuropeptide after traumatic brain injury

2019 Fiscal Year Final Research Report

• Project/Area Number: 16K21372
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Neurosurgery; Emergency medicine
• Research Institution: Showa University
• Principal Investigator: Watanabe Jun 昭和大学, 医学部, 兼任講師 (50649069)
• Project Period (FY): 2016-04-01 – 2020-03-31
• Keywords: 頭部外傷 / 神経新生 / 間葉系幹細胞 / 神経ペプチド

Outline of Final Research Achievements

We investigated the effects of TSG-6 on neuro-regeneration after acute traumatic brain injury. The results clarified the administration of TSG-6 increased the doublecortin positive new born neurons. Then we tried to establish the chronic model of repeated head concussion. However, intracerebral hemorrhagesome caused the animal death in high rate. As the standard deviation of neural death was also huge, we could not fix the model condition. In addition, we tried to make PACAP receptor knock out mouse using crispr CAS9 sysitem. The results indicated the knock out of PACAP causes the death in fetal or new born phase.

Free Research Field

神経再生学

Academic Significance and Societal Importance of the Research Achievements

本研究の研究成果から、間葉系幹細胞の分泌タンパク質であるTSG-6が頭部外傷後の神経の保護作用があるだけでなく、神経新生促進作用もあることが明らかとなった。頭部外傷は現在根治治療の方法がなく、TSG-6が新たな治療法となりうる可能性を示唆した。また、そのメカニズムにPACAPが関与している可能性を示唆した。