Construction of multi-organ simultaneous parallel gene expression network in Cryptomeria japonica.

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K21596
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Forest science; Wood science
• Research Institution: Forest Research and Management Organization
• Principal Investigator: MISHIMA KENTARO 国立研究開発法人森林研究・整備機構, 森林総合研究所林木育種センター, 主任研究員 等 (30438098)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: スギ / 遺伝子発現 / マルチオルガン

Outline of Final Research Achievements

In this study, we have sampled multi-organ (apical-shoot, needle-leaf, cambium) from single tree throughout a year, and then constructed a gene expression network using microarray analysis, which can examine the expression levels of several tens of thousands of genes at the same time. In gene expression data, there were gene groups that do not indicate fluctuation regardless of organ or time, on the other hand, two fluctuation gene expression trends were indicated. Firstly, gene expression pattern was divided into two groups as the cambium group and apical-shoot/needle-leaf group. These findings suggest that difference among two groups indicated in functional difference in tissue. Secondly, gene expression pattern in each group was split into the growing season and dormant season.

Free Research Field

林木育種