Development of micelles mediated fluorescence analysis system

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K21615
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Quantum beam science; Functional solid state chemistry
• Research Institution: 一般財団法人総合科学研究機構(総合科学研究センター(総合科学研究室)及び中性子科学センター(研究開発
• Principal Investigator: Kazuhiro Akutsu 一般財団法人総合科学研究機構(総合科学研究センター(総合科学研究室)及び中性子科学センター(研究開発, 中性子科学センター, 技師 (60637297)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: 蛍光プローブ / XAFS / 中性子反射率 / ミセル

Outline of Final Research Achievements

Because the micelles provide a unique hydrophobic nano-environment for stabilizing metal-organic complexes in aqueous solution, we focused on the unique properties of micelles to develop a new fluorescence analysis system. We synthesized a Sr(II) fluorescent probe, N-(2-hydroxy-3-(1H-benzimidazol-2-yl)-phenyl)-1-aza-18-crown-6-ether (BIC), and revealed that the stability constant of Sr(II)-BIC complex in sodium dodecyl sulfate (SDS) micellar solution was 10 times higher than that in aqueous solution. We also investigated the structure of Sr(II) complex in SDS micellar solution by combining use the extended X-ray absorption fine structure (EXAFS) and neutron reflectivity (NR) method. Based on the EXAFS and NR results, it is clear that Sr(II), BIC, and SDS formed a ternary complexes in SDS micellar solution, and at least, it is clear that the improvement of the stability constant in SDS micellar solution is attributed to the result of the formation of Sr(BIC)(SDS) complex.

Free Research Field

錯体化学