Development of holographic X-ray diffraction imaging method and application to structural study of cell division in an unicellular algae

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K21621
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Biological physics/Chemical physics/Soft matter physics; Quantum beam science
• Research Institution: University of Hyogo
• Principal Investigator: Takayama Yuki 兵庫県立大学, 物質理学研究科, 助教 (40710132)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: コヒーレントX線回折イメージング / X線自由電子レーザー / ホログラフィー / 細胞分裂 / 凍結水和試料

Outline of Final Research Achievements

I developed a holographic diffraction imaging method with an X-ray laser and applied it to structural analysis of primitive red algae cells for understanding of structural basis of cell division, especially focusing on organelle interplay. Imaging red algae cells in interphase and M phase at spatial resolutions of 110 - 200 nm revealed common structural features of organelles and also visualized changes in the electron density distribution in a nucleus associating with cell division without sectioning and/or staining.

Free Research Field

X線イメージング