2019 Fiscal Year Final Research Report
Optical control of transition states in proteins with mid-infrared laser pulses at high intensities
| Project/Area Number |
16KT0163
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 特設分野 |
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| Research Field |
Transition State Control
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| Research Institution | Saitama University (2019)
Osaka University (2016-2018) |
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Principal Investigator |
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| Project Period (FY) |
2016-07-19 – 2020-03-31
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| Keywords | 赤外レーザ / 光受容タンパク質 |
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| Outline of Final Research Achievements |
Several studies have clarified that the transition among quasi-stable species with their characteristic structures is essential for protein functioning. The purposes of this study are 1) clarification of an essential role of hydrogen-bond network in the transitions; 2) optical control of the transitions with mid-infrared laser pulses.
A femtosecond time-resolved spectroscopy system with high sensitivity was developed based on reaction-trigger UV pulse, tunable mid-infrared pump pulse, and broadband probe pulse. Although a signal change induced by the mid-infrared pulse was clearly observed, it is suggested that the change does not come from the contribution of excited state or intermediate states.
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| Free Research Field |
光物性物理学
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| Academic Significance and Societal Importance of the Research Achievements |
タンパク質に光を照射することで、その機能性を向上させたり、抑制したり、あるいは本来とは全く異なる機能を発現することができれば、創薬・医療応用に対する新しいストラテジーを描くことができる。本研究では、タンパク質機能の光制御に関して、準安定状態および準安定状態を制御していると予想される水素結合ネットワークに着目した。赤外光によって、水素結合の構造を変調し、それによってタンパク質の機能を制御できるのではないかという仮説を立てた。結果を得るまでには至らなかったが、仮説検証に必要な様々な要素を明らかにすることができ、今後の研究へと展開する素地ができた。
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