Studies on molecular mechanisms that control development and function of the neural network

2009 Fiscal Year Final Research Report

• Project/Area Number: 17002016
• Research Category: Grant-in-Aid for Specially Promoted Research
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Osaka Bioscience Institute
• Principal Investigator: NAKANISHI Shigetada Osaka Bioscience Institute, 所長 (20089105)
• Co-Investigator(Kenkyū-buntansha): OKAZAWA Makoto (財)大阪バイオサイエンス研究所, システムズ生物学部門, 研究員 (40414130) ; KIWADA Tatsuto (財)大阪バイオサイエンス研究所, システムズ生物学部門, 研究員 (50414131) ; HIKIDA Takatoshi (財)大阪バイオサイエンス研究所, システムズ生物学部門, 研究員 (70421378) ; DOI Tomokazu (財)大阪バイオサイエンス研究所, システムズ生物学部門, 研究員 (20435564) ; YAWATA Satoshi (財)大阪バイオサイエンス研究所, システムズ生物学部門, 研究員 (90455246)
• Project Period (FY): 2005 – 2009
• Keywords: 小脳 / 大脳基底核 / 運動記憶 / 報酬・忌避記憶 / 薬物依存 / 神経回路 / 発達 / 神経伝達

Research Abstract

This project established a novel technique that allowed selective and reversible blockade of specific transmission in the network. The studies using this technique unraveled sequential and integrative processes of acquisition, expression and storage of motor learning at different cerebellar circuits and distinct roles of two parallel pathways of the basal ganglia in rewardbased and aversive learning. This project also disclosed a novel mechanism that controls the activity-dependent maturing processes of the functional cerebellar network organization.

Research Products

• [Journal Article] Ca2+-dependent release of Munc18-1 from presynaptic mGluRs in short-term facilitation (2009)
  • Author(s): Nakajima, Y., Mochida, S., Okawa, K, Nakanishi, S.
  • Journal Title: Proc. Natl. Acad. Sci. USA 106 Pages: 18385-18389
  • Peer Reviewed
• [Journal Article] Role of calcineurin signaling in membrane potential-regulated maturation of cerebellar granule cells (2009)
  • Author(s): Okazawa, M., Abe, H., Katsukawa, M., Iijima, K., Kiwada, T., Nakanishi, S.
  • Journal Title: J. Neurosci. 29 Pages: 2938-2947
  • Peer Reviewed
• [Journal Article] Dual regulation of NR2B and NR2C expression by NMDA receptor activation in mouse cerebellar granule cell cultures (2008)
  • Author(s): Iijima, K., Abe, H., Okazawa, M., Moriyoshi, K., Nakanishi, S.
  • Journal Title: Proc. Natl. Acad. Sci. U.S.A. 105 Pages: 12010-12015
  • Peer Reviewed
• [Journal Article] Conditioned eyeblink learning is formed and stored without cerebellar granule cell transmission (2007)
  • Author(s): Wada, N., Kishimomto, Y., Watanabe, D., Kano, M., Hirano, T., Funabiki, K., Nakanishi, S.
  • Journal Title: Proc. Natl. Acad. Sci. USA 104 Pages: 16690-16695
  • Peer Reviewed
• [Journal Article] Membrane potential-regulated Ca2+ signaling in development and maturation of cerebellar granule cells (2006)
  • Author(s): Nakanishi, S., Okazawa, M.
  • Journal Title: J. Physiol. 575 Pages: 389-395
  • Peer Reviewed
• [Journal Article] Neuronal depolarization controls brain-derived neurotrophic factor-induced upregulation of NR2C NMDA receptor via calcineurin signaling (2005)
  • Author(s): Suzuki, K., Sato, M., Morishima, Y., Nakanishi, S.
  • Journal Title: J. Neurosci. 25 Pages: 9535-9543
  • Peer Reviewed
• [Journal Article] A pivotal role of calcineurin signaling in development and maturation of postnatal cerebellar granule cells (2005)
  • Author(s): Sato, M., Suzuki, K., Yamazaki, H., Nakanishi, S.
  • Journal Title: Proc. Natl. Acad. Sci. USA 102 Pages: 5874-5894
  • Peer Reviewed
• [Journal Article] Enhanced cocaine responsiveness and impaired motor coordination in metabotropic gluamate receptor subtype 2 knockout mice (2005)
  • Author(s): Morishima, Y., Miyakawa, T., Furuyashiki, T., Tanaka, Y., Mizuma, H., Nakanishi, S.
  • Journal Title: Proc. Natl. Acad. Sci. USA 102 Pages: 4170-4175
  • Peer Reviewed
• [Journal Article] Synaptic mechanisms of the cerebellar cortical network (2005)
  • Author(s): Nakanishi, S.
  • Journal Title: Trends Neurosci. 28 Pages: 93-100
  • Peer Reviewed
• [Journal Article] Distinct roles of synaptic transmission in direct and indirect striatal pathways to reward and aversive behavior
  • Author(s): Hikida, T., Kimura, K., Wada, N., Funabiki, K., Nakanishi, S. Neuron
  • Journal Title: (in press)
  • Peer Reviewed
• [Presentation] Integrative and regulatory mechanisms of synaptic transmission in the neural network. (2009)
  • Author(s): Nakanishi, S.
  • Organizer: Key-note lecture, Gordon Research Conference: Neural Circuits & Plasticity
  • Place of Presentation: Salve Regina University, Newport, USA
  • Year and Date: 2009-06-07
• [Presentation] Integrative and regulatory synaptic mechanisms of the cerebellar network. (2008)
  • Author(s): Nakanishi, S.
  • Organizer: The Welcome Trust and Cold Spring Harbor Laboratory joint conference on‘Integrative Approaches to Brain Complexity
  • Place of Presentation: Wellcome Trust Conference Centre, Cambridge, UK
  • Year and Date: 2008-10-02
• [Presentation] Function and signaling mechanism of the mGluR-interacting scaffold tamalin. (2008)
  • Author(s): Nakanishi, S.
  • Organizer: The 6th International Meeting on Metabotropic Glutamate Receptors
  • Place of Presentation: Taormina, Sicily, Italy.
  • Year and Date: 2008-09-14
• [Presentation] The neural network: integration and regulation of synaptic transmission. (2007)
  • Author(s): Nakanishi, S.
  • Organizer: Gruber Prize lecture, Neuroscience 2007
  • Place of Presentation: San Diego Convention Center, San Diego, USA.
  • Year and Date: 2007-11-04
• [Presentation] Integrative and regulatory mechanisms of synaptic transmission in the neural network. (2007)
  • Author(s): Nakanishi, S.
  • Organizer: Key-note lecture, ASPET The Third GPCR Colloquium
  • Place of Presentation: Washington DC Convention Center, Washing ton DC, USA.
  • Year and Date: 2007-04-28
• [Book] The History of Neuroscience in Autobiography (2009)
  • Author(s): Nakanishi, S.
  • Total Pages: 382-419
  • Publisher: Oxford University Press
• [Remarks]
  • URL: http://www.obi.or.jp/index.html
• [Patent(Industrial Property Rights)] 大脳基底核神経回路の神経伝達を解析する方法 (2010)
  • Inventor(s): 疋田貴俊、中西重忠
  • Industrial Property Rights Holder: 財団法人大阪バイオサイエンス研究所
  • Industrial Property Number: 特許・PCT/JP2010/003089
  • Filing Date: 2010-04-30