Signal cascades regulating cell cycle

2009 Fiscal Year Final Research Report

• Project/Area Number: 17012012
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Kyoto University
• Principal Investigator: NISHIDA Eisuke Kyoto University, 大学院・生命科学研究科, 教授 (60143369)
• Project Period (FY): 2005 – 2009
• Keywords: MAPキナ-ゼ / ERK1 / 2 / G0 / G1期 / M期キナーゼ / Plk1 / Myt1 / 細胞分裂期 / 転写ネットワーク

Research Abstract

We have identified two protein kinases, BubR1 and Myt1, as substrates of the M-phase protein kinase Plk1. Plk1 phosphorylation of BubR1 facilitated chromosome alignment during prometaphase while Myt1 was essential for Golgi and ER assembly during mitotic exit. We have also demonstrated that continuous ERK activation in response to growth factor stimulation downregulates antiproliferative genes throughout G1 phase to allow cell cycle progression.

Research Products

• [Journal Article] CrkL is a novel target of Sprouty2 in fibroblast growth factor signaling. (2010)
  • Author(s): Satoh, T., Torii. S., Nakayama, K., Nishida, E.
  • Journal Title: Genes Cells. 15 Pages: 161-168
  • Peer Reviewed
• [Journal Article] Phosphoproteomics reveals new ERK MAP kinase targets and links ERK to nucleoporin-mediated nuclear transport. Nature Struct. (2009)
  • Author(s): Kosako, H., Yamaguchi, N., Aranami, C., Ushiyama, M., Kose, S., Imamoto, N., Taniguchi, H., Nishida, E., Hattori, S.
  • Journal Title: Mol. Biol. 16 Pages: 1026-1035
  • Peer Reviewed
• [Journal Article] Involvement of the protein kinase CK2 in the regulation of mammalian circadian rhythms. (2009)
  • Author(s): Tsuchiya, Y., Akashi, M., Matsuda, M., Goto, K., Miyata, Y., Node, K., Nishida, E.
  • Journal Title: Science Signaling 2 Pages: ra26
  • Peer Reviewed
• [Journal Article] Dual role of Cdc42 in spindle orientation control of adherent cells. (2009)
  • Author(s): Mitsushima, M., Toyoshima, F., Nishida, E.
  • Journal Title: Mol. Cell. Biol. 29 Pages: 2816-2827
  • Peer Reviewed
• [Journal Article] Downregulation of the PI3-kinase/Akt pathway by ERK MAP kinase in growth factor signaling. (2008)
  • Author(s): Hayashi, H., Tsuchiya, Y., Nakayama, K., Satoh, T., Nishida, E.
  • Journal Title: Genes Cells 13 Pages: 941-947
  • Peer Reviewed
• [Journal Article] Ripples from neighbouring transcription. (2008)
  • Author(s): Ebisuya, M., Yamamoto, T., Nakajima, M., Nishida, E.
  • Journal Title: Nature Cell Biol. 10 Pages: 1106-1113
  • Peer Reviewed
• [Journal Article] FGF induces oscillations of Hes1 expression and Ras/ERK activation. (2008)
  • Author(s): Nakayama, K., Satoh, T., Igari, A., Kageyama, R., Nishida, E.
  • Journal Title: Curr. Biol. 18 Pages: 332-334
  • Peer Reviewed
• [Journal Article] Myt1 protein kinase is essential for Golgi and ER assembly during mitotic exit. (2008)
  • Author(s): Nakajima, H., Yonemura, S., Murata, M., Nakamura, N., Piwnica-Worms, H., Nishida, E.
  • Journal Title: J. Cell Biol. 181 Pages: 89-103
  • Peer Reviewed
• [Journal Article] Activation of a C-terminal transcriptional activation domain of ERK5 by autophsophorylation. (2007)
  • Author(s): Morimoto, H., Kondoh, K., Nishimoto, S., Terasawa, K., Nishida, E.
  • Journal Title: J. Biol. Chem. 282 Pages: 35449-35456
  • Peer Reviewed
• [Journal Article] PtdIns(3, 4, 5)P3 Regulates Spindle Orientation in Adherent Cells. (2007)
  • Author(s): Toyoshima, F., Matsumura, S., Morimoto, H., Mitsushima, M., Nishida, E.
  • Journal Title: Dev. Cell 1 Pages: 796-811
  • Peer Reviewed
• [Journal Article] Plk1 facilitates chromosome alignment during prometaphase through BubR1. (2007)
  • Author(s): Matsumura, S., Toyoshima, F., Nishida, E.
  • Journal Title: J. Biol. Chem. 282 Pages: 15217-15227
  • Peer Reviewed
• [Journal Article] Integrin-mediated adhesion orients the spindle parallel to the substratum in an EB1- and myosin X-dependent manner. (2007)
  • Author(s): Toyoshima, F., Nishida, E.
  • Journal Title: EMBO J. 26 Pages: 1487-1498
  • Peer Reviewed
• [Journal Article] Purification of phosphoproteins by immobilized metal affinity chromatography and its application to phosphoproteome analysis. (2007)
  • Author(s): Machida, M., Kosako, H., Shirakabe, K., Kobayashi, M., Ushiyama, M., Inagawa, J., Hirano, J., Nakano, T., Bando, Y., Nishida, E., Hattori, S.
  • Journal Title: FEBS J. 274 Pages: 1576-1587
  • Peer Reviewed
• [Journal Article] Notch signaling suppresses p38 MAPK activity via induction of MKP-1 in myogenesis. (2007)
  • Author(s): Kondoh, K., Sunadome, K., Nishida, E.
  • Journal Title: J. Biol. Chem. 282 Pages: 3058-3065
  • Peer Reviewed
• [Journal Article] MAPK signalling: ERK5 versus ERK1/2. (2006)
  • Author(s): Nishimoto, S., Nishida, E.
  • Journal Title: EMBO Rep. 7 Pages: 782-786
  • Peer Reviewed
• [Journal Article] Continuous ERK Activation Downregulates Antiproliferative Genes throughout G1 Phase to Allow Cell-Cycle Progression. (2006)
  • Author(s): Yamamoto, T., Ebisuya, M., Ashida, F., Okamoto, K., Yonehara, S., Nishida, E.
  • Journal Title: Curr. Biol. 16 Pages: 1171-1182
  • Peer Reviewed
• [Journal Article] Regulation of nuclear translocation of extracellular signal-regulated kinase 5 by active nuclear import and export mechanisms. (2006)
  • Author(s): Kondoh, K., Terasawa, K., Morimoto, H., Nishida, E.
  • Journal Title: Mol. Cell. Biol. 26 Pages: 1679-1690
  • Peer Reviewed
• [Journal Article] Dynamics of the Ras/ERK MAPK cascade as monitored by fluorescent probes. (2006)
  • Author(s): Fujioka, A., Terai, K., Itoh, R.E., Aoki, K., Nakamura, T., Kuroda, S., Nishida, E., Matsuda, M.
  • Journal Title: J. Biol. Chem. 281 Pages: 8917-8926
  • Peer Reviewed
• [Journal Article] Centaurin-alpha1 is a phosphatidylinositol 3- kinase-dependent activator of ERK1/2 mitogen-activated protein kinases. (2006)
  • Author(s): Hayashi, H., Matsuzaki, O., Muramatsu, S., Tsuchiya, Y., Harada, T., Suzuki, Y., Sugano, S., Matsuda, A., Nishida, E.
  • Journal Title: J. Biol. Chem. 281 Pages: 1332-1337
  • Peer Reviewed
• [Journal Article] The duration, magnitude and compartmentalization of ERK MAP kinase activity: mechanisms for providing signaling specificity. (2005)
  • Author(s): Ebisuya, M., Kondoh, K., Nishida, E.
  • Journal Title: J. Cell Sci. 118 Pages: 2997-3002
  • Peer Reviewed
• [Presentation] MAP kinase signaling: Regulatory mechanisms and functions. (2010)
  • Author(s): Nishida, E.
  • Organizer: International Conference on Radiation and Cancer Biology
  • Place of Presentation: Nagasaki University, Nagasaki, Japan
  • Year and Date: 20100217-20100220
• [Presentation] ERK MAP kinase signaling: Regulatory mechganisms and function. (2009)
  • Author(s): Nishida, E.
  • Organizer: International Symposium on Frontiers of Protein Science
  • Place of Presentation: Senri Hankyu Hotel, Osaka, Japan
  • Year and Date: 2009-11-14
• [Presentation] ERK MAP kinase signaling: Regulatory mechganisms and function. (2008)
  • Author(s): Nishida, E.
  • Organizer: 8th International Conference on Protein Phosphatases
  • Place of Presentation: Maebashi TERRSA, Gunma, Japan
  • Year and Date: 20081112-20081114
• [Presentation] Ripples from neighboring transcription (2008)
  • Author(s): Ebisuya, M., Yamamoto, T., Nakajima, M., Nishida, E.
  • Organizer: The 6th International Student Seminar
  • Place of Presentation: Shiran Kaikan, Kyoto University, Kyoto, Japan
  • Year and Date: 20080305-20080306
• [Presentation] Cdc42 and PAK2 Control the Mitotic Spindle Orientation in Mammalian Cells. (2007)
  • Author(s): Mitsushima, M., Toyoshima, F., Nishida, E.
  • Organizer: The 47th Annual Meeting of the American Society for Cell Biology
  • Place of Presentation: Washington Convention Center, Washington DC, USA
  • Year and Date: 20071201-20071205
• [Presentation] Antagonistic Interactions between the Retinoic Acid Receptor and Ras/ERK Signaling Pathways in Colorectal Cancer Cells. (2007)
  • Author(s): Imajo, M., Kondoh, K., Yamamoto, T., Nakayama, K., Nishida, E.
  • Organizer: The 47th Annual Meeting of the American Society for Cell Biology
  • Place of Presentation: Washington Convention Center, Washington DC, USA
  • Year and Date: 20071201-20071205
• [Presentation] Ripples from neighboring transcription. (2007)
  • Author(s): Ebisuya, M., Yamamoto, T., Nishida, E.
  • Organizer: The 7th International Workshop on Advanced Genomics
  • Place of Presentation: Tokyo International Forum, Tokyo, Japan
  • Year and Date: 20071127-20071128
• [Presentation] Regulation of nuclear translocation of ERK5" Kyoto University 21st Century COE program "COE Formation in Frontier Life Science by Unifying Interactions (2006)
  • Author(s): Kondoh, K., Terasawa, K., Morimoto, H., Nishida, E.
  • Organizer: The 4th International Student Seminar
  • Place of Presentation: Kyoudai-Kaikan, Kyoto, Japan
  • Year and Date: 20060306-20060309
• [Presentation] Signal transduction by the ERK family of MAP kinases. (2006)
  • Author(s): Nishida, E.
  • Organizer: International Symposium of Kobe University 21st COE Program on Signal Transduction
  • Place of Presentation: Kobe, Japan
  • Year and Date: 20060209-20060211
• [Presentation] M phase protein kinases and their function. (2005)
  • Author(s): Nishida, E.
  • Organizer: International Symposium on Ran and Cell Cycle
  • Place of Presentation: Awaji, Japan
  • Year and Date: 20051002-20051004
• [Presentation] FGF signaling regulates the oscillatory expression of Hes1 in cultured cells (2005)
  • Author(s): Nakayama, K., Kondoh, K., Kageyama, R., Nishida, E.
  • Organizer: he 15th International Society of Developmental Biologists Congress
  • Place of Presentation: Sydney, Australia
  • Year and Date: 20050903-20050907