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2009 Fiscal Year Final Research Report

Analysis on the spatio-temporal regulation of cellular oncogenesis

Research Project

  • PDF
Project/Area Number 17012014
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionKyoto University (2006-2009)
Osaka University (2005)

Principal Investigator

MATSUDA Michiyuki  Kyoto University, 大学院・生命科学研究科, 教授 (10199812)

Co-Investigator(Kenkyū-buntansha) NAKAMURA Takeshi  東京理科大学, 生命科学研究所, 教授 (60362604)
KIYOKAWA Etsuko  京都大学, 大学院・医学研究科, 講師 (80300929)
Project Period (FY) 2005 – 2009
Keywordsがん / FRETバイオセンサー / 蛍光イメージング / システム生物学
Research Abstract

We aimed at understanding the oncogene signal transduction network. For this purpose, we have developed biosensors based on the principle of fluorescence resonance energy transfer (FRET) and developed simulation models. These models will be bases of further improved simulation models, which allow us to draw the global picture of oncogene signal transduction network.

  • Research Products

    (20 results)

All 2011 2010 Other 0

All Journal Article (3 results) Presentation (14 results) Remarks (3 results)

  • [Journal Article] A genetically encoded Forster resonance energy transfer biosensor for two-photon excitation microscopy2011

    • Author(s)
      Kumagai, Y.; Kamioka, Y.; Yagi, S.; Matsuda, M.; Kiyokawa, E.
    • Journal Title

      Analytical Chemistry 413(2)

      Pages: 192-199

  • [Journal Article] Multiple decisive phosphorylation sites for the negative feedback regulation of SOS1 via ERK2010

    • Author(s)
      Kamioka, Y.; Yasuda, S.; Fujita, Y.; Aoki, K.; Matsuda, M.
    • Journal Title

      Journal of Biological Chemistry 285(43)

      Pages: 33540-33548

  • [Journal Article] Heterogeneity of phosphatidic acid levels and distribution at the plasma membrane in living cells as visualized by a Foerster resonance energy transfer (FRET) biosensor2010

    • Author(s)
      Nishioka, T.; Frohman, M.A.; Matsuda, M.; Kiyokawa, E.
    • Journal Title

      Journal of Biological Chemistry 285(46)

      Pages: 35979-35987

  • [Presentation] 上皮管腔形成におけるRac1活性パターンの可視化と制御2011

    • Author(s)
      八木俊輔、松田道行、清川悦子
    • Organizer
      第一回新学術領域研究「蛍光生体イメージ」Vivid Workshop
    • Place of Presentation
      蓼科
    • Year and Date
      2011-03-10
  • [Presentation] Monitoring of phosphatidic acid distribution in the living cells by FRET probes2010

    • Author(s)
      岡照子、Michael A.Frohman、清川悦子、松田道行
    • Organizer
      第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
    • Place of Presentation
      神戸
    • Year and Date
      20101207-20101210
  • [Presentation] Quantitative kinetic analysis revealed processive phosphorylation of ERK MAP kinase in mammalian cells2010

    • Author(s)
      Kazuhiro Aoki, Michiyuki Matsuda
    • Organizer
      第33回日本分子生物学会年会・第82回日本生化学会大会合同大会
    • Place of Presentation
      神戸
    • Year and Date
      20101207-20101210
  • [Presentation] Regulation of Transcriptomes by Stochastic Change in Rho-family GTPase Activity2010

    • Author(s)
      幸長弘子、平田英周、程久美子、松田道行
    • Organizer
      グローバルCOE国際シンポジウム/リトリート2010
    • Place of Presentation
      淡路
    • Year and Date
      20101105-20101106
  • [Presentation] Transcriptome Analysis of Glioblastoma Cells Expressing FRET Biosensors for GTPases2010

    • Author(s)
      幸長弘子、平田英周、程久美子、松田道行
    • Organizer
      第62回日本細胞生物学会大会
    • Place of Presentation
      大阪
    • Year and Date
      20100519-20100521
  • [Presentation] Regulation of Transcriptomes by Stochastic Change in Rho-family GTPase Activity.2010

    • Author(s)
      Hiroko Yukinaga, Eishu Hirata, Kumiko Ui-Tei, Michiyuki Matsuda
    • Organizer
      Amrican society for Cell Biology 50th Annual Meeting
    • Year and Date
      2010-12-15
  • [Presentation] FRETイメージングと統計的信号解析に基づく自発的な細胞遊走を制御する分子ネットワークの解析2010

    • Author(s)
      国田勝行、青木一洋、松田道行
    • Organizer
      第83回日本生化学会大会
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-07
  • [Presentation] Rational design of intramolecular FRET biosensors for monitoring Ser/Thr kinase activity2010

    • Author(s)
      Naoki Komatsu, Kazuhiro Aoki, Michiyuki Matsuda
    • Organizer
      APRU Research Symposium
    • Place of Presentation
      京都
    • Year and Date
      2010-11-25
  • [Presentation] 管腔構造形成過程における信号伝達分子のFRETによる可視化2010

    • Author(s)
      八木俊輔、松田道行、清川悦子
    • Organizer
      第82回日本生化学会大会
    • Place of Presentation
      神戸
    • Year and Date
      2010-10-22
  • [Presentation] PC12D細胞における2010

    • Author(s)
      後藤明弘、中村岳史、星野幹雄、松田道行
    • Organizer
      dbcAMP及びNGFによって誘導される神経突起生成のシグナル伝達経路のFRETを用いた解析Neuro 2010(第33回日本神経科学大会・第53回日本神経科学会大会・第20回日本神経回路学会大会合同大会)
    • Place of Presentation
      神戸
    • Year and Date
      2010-09-03
  • [Presentation] Development of a FRET-based system monitoring Ser/Thr kinase activity in living cells2010

    • Author(s)
      小松直樹、青木一洋、松田道行
    • Organizer
      第62回日本細胞生物学会大会
    • Year and Date
      2010-05-21
  • [Presentation] Development of a FRET-based system monitoring Ser/Thr kinase activity in living cells2010

    • Author(s)
      Naoki Komatsu, Kazuhiro Aoki, Michiyuki Matsud
    • Organizer
      第62回日本細胞生物学会大会
    • Place of Presentation
      大阪
    • Year and Date
      2010-05-21
  • [Presentation] Development of FRET probes for two-photon excitation microscopy2010

    • Author(s)
      熊谷悠香、清川悦子、松田道行
    • Organizer
      第62回日本細胞生物学会大会
    • Place of Presentation
      大阪
    • Year and Date
      2010-05-19
  • [Presentation] Identification of molecular network based on FRET imaging and statistical signal processing in randomly migrating HT1080 cells0

    • Author(s)
      国田勝行、青木一洋、松田道行
    • Organizer
      第48回日本生物物理学会年会
    • Place of Presentation
      東北大学
    • Year and Date
      00000920
  • [Remarks] ホームページ等

  • [Remarks] 研究内容

    • URL

      http://www.lif.kyoto-u.ac.jp/labs/fret/project.htm

  • [Remarks] 発表論文

    • URL

      http://www.lif.kyoto-u.ac.jp/labs/fret/publist.htm

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Published: 2012-02-13   Modified: 2016-04-21  

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