個体レベルでのがんの統合的研究

2005 Fiscal Year Annual Research Report

• Project/Area Number: 17012017
• Research Institution: Kumamoto University
• Principal Investigator: 山村 研一 熊本大学, 発生医学研究センター, 教授 (90115197)
• Co-Investigator(Kenkyū-buntansha): 伊藤 豊志雄 (財)実験動物中央研究所, 動物医学研究室, 室長 (20106644) ; 加藤 秀樹 浜松医科大学, 医学部附属動物実験施設, 助教授 (30142053) ; 中尾 和貴 理研, 発生・再生研・変異マウス開発チーム, 研究員 (20217657) ; 小幡 裕一 (独)理研, バイオリソースセンター, センター長 (30177290) ; 山崎 由紀子 情報・システム研究機構・国立遺伝学研究所, 助教授 (00239956)
• Keywords: トランスジェニック / ノックアウト / 相同組換え / 遺伝子トラップ / 凍結保存 / 微生物学的モニタリング / 遺伝学的モニタリング / データベース

Research Abstract

フィーダーフリーのTT2 ES細胞を樹立した。ICRのモルラ胚との凝集法でキメラ作製が可能である。また、C57BL/6由来のES細胞の樹立し、100%キメラが得られた。しかし、生殖系列への伝達率は、TT2に比較し悪い。トランスジェニック及びキメラマウス作製を、計244件実施した。ES細胞相同組換えに関する相談は、17件にのぼった。その内容は、ベクター構築に関してパーツとなるプラスミドや部位特異的組換えに関するプラズミドの分与、feeder free ES細胞の分与、ベクター構築(アームの長さや場所等)の相談、ES細胞培養技術指導、ES細胞へのベクター導入とクローン単離、キメラマウス樹立およびマウス交配指導、トラップES細胞を利用した遺伝子置換のベクター構築の指導等であった。37機関から361件の微生物学的モニタリングの依頼があり、4608検体の検査を行った。マウス肝炎ウイルスの感染はほとんど見られなくなった。海外へのマウスの送付が増加しているため、英語での検査成績依頼が増加している。リボゾーマルRNA遺伝子は13箇所に別れて遺伝子座が存在する。このような場合、トラップベクターが挿入されたリボゾーマル遺伝子の位置を容易に特定できない。そこで、交配による遺伝子マッピングを行い、18番または19番染色体上にあることを、明らかにした。遺伝子操作マウスの寄託凍結保存数は767件、供給系統数は950件であった。MHVに感染したマウス等500系統ののクリーニングを行った。生殖工学に関する技術指導を国内では4回20名、国外では中国北京大学で5名について行った。生殖工学の技術を網羅した英語のCD版を作製した。凍結精子の体外受精用の培地を開発した。CARD R-BASEから、EGTC、JMSR、FIMRE、IMSRへのリンクを張り、より利用しやすい環境を作った。

Research Products

• [Journal Article] The international gene trap consortium web site : a portal to all publly available gene trap cell lines and mouse. (2006)
  • Author(s): Nord, A.S.et al.
  • Journal Title: Nucleic Acids Res. 34 Pages: D642-D648
• [Journal Article] A novel murine gene, Sickele tail (Skt), linked to the Danforth's shot tail (Sd) locus, is required for normal development of the intervertebral disc. (2006)
  • Author(s): Semba, K. et al.
  • Journal Title: Genetics 172 Pages: 445-456
• [Journal Article] Impaired expression of Importin/karyopherin β 1 leads to post-implantation lethality. (2006)
  • Author(s): Miura, K et al.
  • Journal Title: Biochem Biophys Res Commun. 341 Pages: 132-138
• [Journal Article] Characterization of an exchangeable gene trap using pU-17 carrying a stop codon-betageo cassette. (2005)
  • Author(s): Taniwaki, T., et al.
  • Journal Title: Dev.Growth Differ. 47 Pages: 163-172
• [Journal Article] Autophagic cell death of pancreastic acinar cells in serine protease inhibitor Kazal Type3 deficient mice. (2005)
  • Author(s): Ohmuraya, M. et al.
  • Journal Title: Gastroenterology 129 Pages: 696-705
• [Journal Article] Sonic hedgehog is required for cardiac outflow, tract and neural crest cell development. (2005)
  • Author(s): Washington, Smoka. et al.
  • Journal Title: Dev. Biol. 283 Pages: 357-372
• [Journal Article] Presence and distribution of neural crest-derived cells in the murine developing thymus and their potential for differentiation. (2005)
  • Author(s): Yamazaki, H., et al.
  • Journal Title: Int. Immunol. 17 Pages: 549-558
• [Journal Article] Animal model of occulopharyngeal muscular dystrophy. (2005)
  • Author(s): Uyama, E., et al.
  • Journal Title: Acta Myologica XXIV Pages: 84-88
• [Journal Article] Increase of smooth muscle cell migration and of intimal hyperplasia in mice lacking the abhydrolase domain containing 2 gene. (2005)
  • Author(s): Miyata, K., et al.
  • Journal Title: Biochem Biophys Res Commun. 329 Pages: 296-304
• [Journal Article] Reproductive/urogenital organ development and the molecular genetic cascades ; glamorous developmental processes of bodies. (2005)
  • Author(s): Yamada, G.
  • Journal Title: J Biochem (Tokyo). 137 Pages: 665-669
• [Journal Article] TAK1, but not TAB1 or TAB2, plays an essential role in multiple signaling pathways in vivo. (2005)
  • Author(s): Shim, JH., et al.
  • Journal Title: Genes Dev. 19(22) Pages: 2668-2681
• [Journal Article] The ventral abdominal wall dysmorphogenesis of Msx1/Msx2 double-mutant mice. (2005)
  • Author(s): Ogi, H., et al.
  • Journal Title: Anatomical Record 31;284A Pages: 424-430
• [Journal Article] The morphological and histological characters of the male external genitalia of the house musk shrew, Suncus murinus. (2005)
  • Author(s): Kamikawa-Miyado, M., et al.
  • Journal Title: Zoolog Sci. 22 Pages: 463-468
• [Journal Article] Hepatocyte Growth Factor Activator Inhibitor Type 1 (HAI-1) is Required for Branching Morphogenesis in the Chorioallantonic Placenta. (2005)
  • Author(s): Tanaka, H., et al.
  • Journal Title: Mol. Cell. Biol. 25 Pages: 5687-5698
• [Journal Article] The cellular and molecular etiology of the cleft secondary palate in Fgf10 mutant mice. (2005)
  • Author(s): Alappat, SR., et al.
  • Journal Title: Dev Biol. 277 Pages: 102-113
• [Journal Article] Exchangeable gene trapping. in Genetically Engineered Mice (eds Sundberg, J. P. and Ichiki, T.) (2005)
  • Author(s): Araki, K.
  • Journal Title: CRC Press, Boca Raton, USA. Pages: 131-143
• [Journal Article] Microarray analysis of the genes induced by tetracycline-regulated expression of NDRF/NeuroD2 in P19 cells. (2005)
  • Author(s): Oda, H., Fushimi, F., Kato, M., Kitagawa, M., Araki, K., Seki, N., Ohkubo, H.
  • Journal Title: Biochem Biophys Res Commun. 335 Pages: 458-468
• [Journal Article] Development of Autoimmunity against Transcriptionally Unrepressed Target Antigen in the Thymus of Aire-Deficient Mice. (2005)
  • Author(s): Kuroda, N., et al.
  • Journal Title: Anatomical Record 174(4) Pages: 1862-1870
• [Journal Article] Genetic diversity on 16S rDNA sequence and phylogenic tree analysis in Pasteurella pneumotropica strains isolated from laboratory animals. (2005)
  • Author(s): Hayashimoto, N., et al.
  • Journal Title: Current Microbiol. 51 Pages: 1-6
• [Journal Article] Identification procedure for Pasteurella pneumotropica in microbiologic monitoring of laboratory animals. (2005)
  • Author(s): Hayashimoto, N., et al.
  • Journal Title: Exp.Anim. 54 Pages: 123-129
• [Journal Article] The Wilms' tumor gene WT1 is a common marker of progenitor cells in fetal liver. (2005)
  • Author(s): Kanato, K., et al.
  • Journal Title: Biochem Biophys Res Commun. 28:326(4) Pages: 836-843
• [Journal Article] Angiopoietin-related growth factor antagonizes obesity and insulin resistance. (2005)
  • Author(s): Oike, Y., et al.
  • Journal Title: Nat Med. 11(4) Pages: 400-408
• [Journal Article] Ovarian follicular development stimulated by leuprorelin acetate plus human menopausal gonadotropin in chimpanzees. (2005)
  • Author(s): Yoshimoto, N., et al.
  • Journal Title: J Med Primatol. 34(2) Pages: 73-85
• [Journal Article] Relation between storage temperature and fertilizing ability of freeze-dried mouse spermatozoa. (2005)
  • Author(s): Kaneko, T., Nakagata, N.
  • Journal Title: Comp Med. 55(2) Pages: 140-144
• [Journal Article] Use of frozen-thawed oocytes for efficient production of normal offspring from cryopreserved mouse spermatozoa showing low fertility. (2005)
  • Author(s): Sakamoto, W., Kaneko, T., Nakagata, N.
  • Journal Title: Comp Med. 55(2) Pages: 136-139
• [Journal Article] Canonical Wnt signaling and its antagonist regulate anterior-posterior axis polarization by guiding cell migration in mouse visceral endoderm. (2005)
  • Author(s): Kimura-Yoshida, C., et al.
  • Journal Title: Dev Cell. 9 Pages: 639-650
• [Journal Article] Farnesylation of retinal transducin underlies its translocation during light adaptation. (2005)
  • Author(s): Kassai, H., et al.
  • Journal Title: Neuron. 47 Pages: 529-539
• [Journal Article] A gene-targeted mouse model for chorea-acanthocytosis. (2005)
  • Author(s): Tomemori, Y., et al.
  • Journal Title: J Neurochem. 92 Pages: 759-766
• [Journal Article] Vascular endothelial growth factor A (VEGF-A) is involved in guidance of VEGF receptor-positive cells to the anterior portion of early embryos. (2005)
  • Author(s): Hiratsuka, S., et al.
  • Journal Title: Mol Cell Biol. 25 Pages: 355-363
• [Journal Article] Membrane fixation of vascular endothelial growth factor receptor 1 ligand-binding domain is important for vasculogenesis and angiogenesis in mice. (2005)
  • Author(s): Hiratsuka, S., et al.
  • Journal Title: Mol Cell Biol. 25 Pages: 346-354
• [Journal Article] Microsatellite genotyping for genetic quality testing using sperm cells in the mouse. (2005)
  • Author(s): Katoh.H., Yoshino, S., Inui, Y., Honda, S., Takabayashi, S.
  • Journal Title: Exp. Anim. 54 Pages: 373-376
• [Journal Article] A mutant mouse with severe anemia and skin abno rmalities controlled by a new allele of the flaky skin (fsn) locus. (2005)
  • Author(s): Takabayashi, S., Katoh, H.
  • Journal Title: Exp. Anim. 54 Pages: 339-347
• [Journal Article] Cell size and nucleoid organization of engineered Escherichia coli cells with a reduced genome. (2005)
  • Author(s): Hashimoto, M., et al.
  • Journal Title: J.Mol. Microbiol. 55(1) Pages: 137
• [Journal Article] Structural dynamics of cereal mitochondrial genomes as revealed by complete nucleotide sequenceing of the wheat mitochondrial genome. (2005)
  • Author(s): Ogihara, Y., et al.
  • Journal Title: Nucleic Acids Res. 33(19) Pages: 6235
• [Journal Article] Functional Isolation of novel nuclear proteins showing a variety of subnuclear localizations. (2005)
  • Author(s): Moriguchi, K., Suzuki, Y., Ito, Y., Yamazaki.Y., Niwa, Y., Kurata, N
  • Journal Title: The Plant Cell. 17 Pages: 389
• [Journal Article] Biological ontologies in rice databases. (2005)
  • Author(s): Yamazaki, Y., Jaiswal, P.
  • Journal Title: Plant and Cell Physiology. 46 Pages: 63
• [Journal Article] Rice mutants and genes related to organ development, morphogenesis and physiological traits, (2005)
  • Author(s): Kurata, N., Miyoshi, K., Nonomura, K.-I., Yamazaki, Y., Ito, Y.
  • Journal Title: Plant and Cell Physiology 46 Pages: 48
• [Journal Article] Transient strong reduction of PTEN expression by specific RNAi induces loss of adhesion of the cells. (2005)
  • Author(s): Mise-Omata, S., Obata, Y., Iwase, S., Mise, N., Doi, S.T.
  • Journal Title: Biochem.Biophy. Res. Commun. in press