Integrated cancer research using in vivo models

2009 Fiscal Year Final Research Report

• Project/Area Number: 17012017
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Kumamoto University
• Principal Investigator: YAMAMURA Kenichi Kumamoto University, 生命資源研究・支援センター, 教授 (90115197)
• Co-Investigator(Kenkyū-buntansha): YAMADA Gen 熊本大学, 発生医学研究所, 教授 (80174712) ; ARAKI Kimi 熊本大学, 生命資源研究・支援センター, 准教授 (90211705) ; ITOH Toshio 財団法人実験動物中央研究所, 動物医学研究室, 室長 (20106644) ; KATOH Hideki 浜松医科大学, 動物実験施設, 准教授 (30142053) ; NAKAGATA Naomi 熊本大学, 生命資源研究・支援センター, 教授 (30159058) ; NAKO Kazuki 独立行政法人理化学研究所, 発生・再生科学総合研究センター・動物資源開発・動物実験支援ユニット, ユニットリーダー (20217657) ; OBATA Yuichi 独立行政法人理化学研究所, バイオリソースセンター, センター長 (30177290) ; YAMAZAKI Yukiko 国立遺伝学研究所, 准教授 (00239956) ; MATSUI Yasuhisa 医用細胞資源センター, 教授 (40241575) ; WANIBUCHI Hideki 大阪市立大学, 大学院・医学研究科, 教授 (90220970) ; FUKAMACHI Hiroshi 東京医科歯科大学, 大学院・医歯学総合研究科, 講師 (70134450) ; MITSUMORI Kunitoshi 東京農工大学, 大学院・農学研究院, 教授 (10239296) ; USHIJIMA Toshikazu 国立がんセンター(研究所及び東病院臨床開発センター), 発がん研究部, 部長 (90232818) ; OGAWA Katsuhiro 旭川医科大学, 病理学講座腫瘍病理分野, 名誉教授 (50045514) ; TATEMATSU Masae 愛知がんセンター研究所, 腫瘍病理部, 副所長 (70117836)
• Project Period (FY): 2005 – 2009
• Keywords: 遺伝子改変マウス / 相同組換え / 凍結保存 / 微生物学的モニタリング / 遺伝学的モニタリング / データベース / 病理形態学 / 組織診断

Research Abstract

We organized 7 groups to support cancer research in Japan during 2005 and 2009 academic year. We produced 1463 transgenic or knockout mouse strains and responded to 191 consultations by distributing materials such as targeting vectors. The number of request for conditional gene targeting instead of simple knockout is increasing in recent years. Microbiological monitoring and genetic monitoring using biochemical/microsattelite markers were accomplished for 25,769 samples in 1798 cases and 1,367 samples, respectively. Due to these efforts, infection to laboratory mouse is rarely seen under SPF condition in these years. We cryopreserved embryos and sperms for 7,985 strains and supplied 4,358 strains. Infected 2739 strains were recovered and cleaned from viruses using in vitro fertilization technique. We started to supply embryos under refrigerated condition using a new method by which recovered embryo can be preserved for 3 or 4 days. We held the course for reproductive engineering for 24 times to 136 trainees. CARD-RBASE was established with necessary function such as links to other web sites. We supplied 7,357 cell lines for cancer researchers. We also carried out pathologic analyses for 55 genetically engineered mouse strains upon request.

Research Products

• [Journal Article] National BioResource Project Information Center. (2009)
  • Author(s): Yamazaki, Y., Sugawara, H.
  • Journal Title: Experimental Animals 58巻 Pages: 75-84
  • Peer Reviewed
• [Journal Article] Birth of mice from vitrified/warmed 2-cell embryos transported at a cold temperature. (2009)
  • Author(s): Takeo T, Kaneko T, Haruguchi Y, 他15名15番目
  • Journal Title: Cryobiology 58巻 Pages: 196-202
  • Peer Reviewed
• [Journal Article] Embryonic hair follicle fate change by augmented β-catenin through Shh and Bmp signaling. (2009)
  • Author(s): Suzuki, K., Yamaguchi, Y., Villacorte, M., Mihara, M., Akiyama, M., Shimizu, H., Taketo, M.M., Nakagata, N., Tsukiyama, T., Yamaguchi, T.P., Birchmeier, W., Kato, S., Yamada, G.
  • Journal Title: Development 136巻 Pages: 367-372
  • Peer Reviewed
• [Journal Article] Molecular detection of murine norovirus from experimentally and spontaneously infected mice. (2009)
  • Author(s): Kazuo Goto, Nobuhito Hayashimoto, Masahiko Yasuda, Tomoko Ishida, Shuko Kameda, Akira Takakura, Toshio Itoh.
  • Journal Title: Exp. Anim. 58 Pages: 135-140
  • Peer Reviewed
• [Journal Article] The Mouse resources at the RIKEN BioResource Center. (2009)
  • Author(s): Yoshiki, A., Ike, F., Mekada, K., Kitaura, Y., Nakata, H., Hiraiwa, N., Keiji, M., Ijuin, M., Kadota, M., Murakami, A., Ogura, A., Abe, K., Moriwaki, K., Obata, Y.
  • Journal Title: Exp. Anim. 58巻 Pages: 85-96
  • Peer Reviewed
• [Journal Article] Gene trap mutagenesis in mice: New perspectives and tools in cancer research. (2008)
  • Author(s): Yamamura, K., Araki, K.
  • Journal Title: Cancer Sci Vol.99 Pages: 40549
  • Peer Reviewed
• [Journal Article] Epigenetic events in mammalian germ-cell development: reprogramming and beyond (2008)
  • Author(s): Sasaki H, Matsui Y
  • Journal Title: Nat Rev Genet 9巻 Pages: 129-140
  • Peer Reviewed
• [Journal Article] 4-Vinyl-2, 6-dimethoxyphenol (canolol) suppresses oxidative stress and gastric carcinogenesis in Helicobacter pylori-infected carcinogen-treated Mongolian gerbils. (2008)
  • Author(s): Cao X, Tsukamoto T, Seki T, Tanaka H, Morimura S, Cao L, Mizoshita T, Ban H, Toyoda T, Maeda H, Tatematsu M
  • Journal Title: Int J Cancer 122巻 Pages: 1445-1454
  • Peer Reviewed
• [Journal Article] The novel tetratricopeptide repeat domain 7 mutations, Ttc7fsn-Jic, with deletion of the TPR-2B repeat causes severe flaky skin phenotype. (2007)
  • Author(s): Takabayashi, S., Iwashita, S., Hirashima, T., Katoh, H
  • Journal Title: Exp Bio Med 232巻 Pages: 695-699
  • Peer Reviewed
• [Journal Article] Neuroepithelial cells supply an initial transient wave of MSC differentiation. (2007)
  • Author(s): Takashima, Y., Era, T., Nakao, K., Kondo, S., Kasuga, M., Smith, AG., Nishikawa, S.
  • Journal Title: Cell 129巻 Pages: 1377-1388
  • Peer Reviewed
• [Journal Article] A specific plymerase chain reaction based on the gyrB gene sequence and subsequent restriction fragment length polymorphism analysis of Pasteurella pneumotropica isolates from laboratory mice. (2006)
  • Author(s): Hayashimoto, N., Ueno, M., Takakura, A., Itoh, T.
  • Journal Title: J. Amer. Assoc. Lab. Anim. Sci. 46巻 Pages: 54-58
  • Peer Reviewed
• [Presentation] 凍結融解マウス胚の冷蔵輸送 (2009)
  • Author(s): 中潟直己、古波蔵恵里, 他10名
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 20091209-20091212
• [Presentation] ES細胞においてlox71部位に対して良い組換え効率を示す新たなRE変異loxの探索 (2007)
  • Author(s): 荒木喜美, 岡田由香, 荒木正健, 山村研一
  • Organizer: 第40回日本発生生物学会・第50回日本細胞生物学会合同大会
  • Place of Presentation: 福岡
  • Year and Date: 20070528-20070530
• [Presentation] マウスクローズドコロニーに内在する自然突然変異遺伝子 (2006)
  • Author(s): 加藤秀樹、木村二郎、西川哲、高林秀次
  • Organizer: 第53回日本実験度物学会総会
  • Place of Presentation: 神戸
  • Year and Date: 20060500
• [Presentation] 理研バイオリソースセンターにおけるマウス系統基礎特性検査について (2006)
  • Author(s): 目加田和之、山崎京子、斉藤昭男、中村哲枝、村上亜弓、森脇和郎、小幡裕一、吉木淳
  • Organizer: 第53回日本実験動物学会総会
  • Place of Presentation: 神戸
  • Year and Date: 20060500
• [Book] Genetically Engineered Mice(eds Sundberg. J.P. & Ichiki T., Boca Raton, USA. Exchangeable gene trapping.) (2005)
  • Author(s): Araki, K.
  • Total Pages: 131-143
  • Publisher: CRC Press
• [Remarks] 熊本大学生命資源研究支援センター
  • URL: http://www.irda.kumamoto-u.ac.jp/
• [Remarks] 東北大学加齢医学研究所附属医用細胞資源センターから供給可能な細胞株
  • URL: http://www.idac.tohoku.ac.jp/dep/ccr/
• [Remarks] 日本で維持されている可移植性腫瘍株一覧表
  • URL: http://www.idac.tohoku.ac.jp/dep/ccr/
• [Patent(Industrial Property Rights)] トラップベクターおよびこれを用いた遺伝子トラップ法 (2010)
  • Inventor(s): 山村研一、荒木喜美
  • Industrial Property Rights Holder: 株式会社トランスジェニック
  • Industrial Property Number: 特許・EP1201759
  • Filing Date: 2010-03-10
• [Patent(Industrial Property Rights)] トラップベクターおよびこれを用いた遺伝子トラップ法 (2007)
  • Inventor(s): 山村研一、荒木喜美
  • Industrial Property Rights Holder: 株式会社トランスジェニック
  • Industrial Property Number: 特許・US7312075
  • Filing Date: 2007-12-25