Reverse Genetic Analysis of DNA Damage responses in the Avian DT40 Cell Line and the Medaka Fish

2009 Fiscal Year Final Research Report

• Project/Area Number: 17013039
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Kyoto University
• Principal Investigator: TAKEDA Shunichi Kyoto University, 大学院・医学研究科, 教授 (60188191)
• Co-Investigator(Kenkyū-buntansha): TANIGUCHI Yoshihito 京都大学, 大学院・医学研究科, 准教授 (00324616)
• Project Period (FY): 2005 – 2009
• Keywords: 癌 / ゲノム / 細胞・組織 / バイオテクノロジー / 放射線

Research Abstract

A number of anti-cancer agents, including radiotherapy, induce DNA damage, arrest DNA replication, and thereby induce apoptosis. Therefore, functional analysis of DNA repair factors may contribute to improvement of anti-cancer treatments. For this functional analysis, we have developed unique reverse genetic model systems, the avian somatic cell line (DT40) and the Medaka fish. We have revealed the role of ubiquitylation, some DNA polymerases, and BRCA1/2 in DNA damage responses.

Research Products

• [Journal Article] accumulation of RAD51 at double-strand (2010) GEMIN2 promotes breaks in homologous recombination. (2010)
  • Author(s): Takizawa Y, Qing Y, Takaku M, Ishida T, Morozumi Y, Tsujita T, Kogame T, Hirota K, Takahashi M, Shibata T, Kurumizaka H, Takeda S.
  • Journal Title: Nucleic Acids Res. (in press)
  • Peer Reviewed
• [Journal Article] DNA polymerasesνandθare Takeda S. required for efficient Immunoglobulin V gene diversification in chicken. (2010)
  • Author(s): Kohzaki M, Nishihara K, Hirota K, Sonoda E, Yoshimura M, Ekino S, Butler JE, Watanabe M, Halazonetis T
  • Journal Title: J C e l l B i o l. (in press)
  • Peer Reviewed
• [Journal Article] Collaborative action of Brca1 Taniguchi and CtIP in elimination of covalent modifications from double-strand breaks to facilitate subsequent break repair. (2010)
  • Author(s): Nakamura K, Kogame T, Oshiumi H, Shinohara A, Sumitomo Y, Agama K, Pommier Y, Tsutsui KM, Tsutsui K, Hartsuiker E, Ogi T, Takeda S, Y.
  • Journal Title: P L o S G e n e t. 6 Pages: e1000828
  • Peer Reviewed
• [Journal Article] PTIP promotes DNA double-strand break repair through homologous recombination. (2010)
  • Author(s): Wang X, Takenaka K, Takeda S.
  • Journal Title: G e n e s C e l l s (in press)
  • Peer Reviewed
• [Journal Article] approach using DNA-repair-deficient (2010) A novel chicken DT40 cell lines for screening and characterizing the genotoxicity of environmental contaminants. (2010)
  • Author(s): Ji K, Kogame T, Choi K, Wang X, Lee J, Taniguchi Y, Takeda S.
  • Journal Title: Environ Health Perspect. 117 Pages: 1737-44
  • Peer Reviewed
• [Journal Article] Bloom DNA helicase facilitates Hirota K. (2009) homologous recombination between diverged homologous sequences. (2009)
  • Author(s): Kikuchi K, Abdel-Aziz HI, Yamazoe M, Taniguchi Y, Takeda S
  • Journal Title: J B i o l C h e m. 284 Pages: 26360-7
  • Peer Reviewed
• [Journal Article] the non-homologous end-joining repair Okada N. (2009) Genetic evidence that pathway is involved in LINE retrotransposition. (2009)
  • Author(s): Suzuki J, Yamaguchi K, Kajikawa M, Ichiyanagi K, Adachi N, Koyama H, Takeda S
  • Journal Title: P L o S G e n e t. 5 Pages: e1000461
  • Peer Reviewed
• [Journal Article] An ATM and ATR Takeda S, dependent checkpoint inactivates spindle assembly by targeting CEP63. (2009)
  • Author(s): Smith E, Dejsuphong D, Balestrini A, Hampel M, Lenz C, Vindigni A, Costanzo V.
  • Journal Title: N a t C e l l B i o l. 11 Pages: 278-85
  • Peer Reviewed
• [Journal Article] for single-strand lesions initiating (2009) Genetic evidence Nbs1-dependent homologous recombination in diversification of Ig V in chicken B lymphocytes. (2009)
  • Author(s): Nakahara M, Sonoda E, Nojima K, Sale JE, Takenaka K, Kikuchi K, Nakamura K, Sumitomo Y, Bree RT, Lowndes Taniguchi Y, NF, Takeda S.
  • Journal Title: P L o S G e n e t. 5 Pages: e1000356
  • Peer Reviewed
• [Journal Article] Cohesin associates with Fukagawa T, Yen TJ, Yokomori spindle poles in a mitosis-specific manner and functions in spindle assembly in vertebrate cells. (2009)
  • Author(s): Kong X, Ball AR, Jr., Sonoda E, Feng J, Takeda S, K.
  • Journal Title: M o l B i o l C e l l. 20 Pages: 1289-301
• [Journal Article] Cyclin-dependent kinases and cell-cycle Hunt T. transitions: does one fit all? (2008)
  • Author(s): Hochegger H, Takeda S
  • Journal Title: Nat Rev M o l C e l l B i o l 9(Review) Pages: 910-6
  • Peer Reviewed
• [Journal Article] PARP-1 ensures the modulation of replication Okumura K. fork progression by homologous recombination on damaged DNA. (2008)
  • Author(s): Sugimura K, Takebayashi S, Taguchi H, Takeda S
  • Journal Title: J C e l l B i o l. 183 Pages: 1203-12
  • Peer Reviewed
• [Journal Article] The 9-1-1 DNA clamp is Takeda S, required for immunoglobulin gene conversion. (2008)
  • Author(s): Saberi A, Nakahara M, Sale JE, Kikuchi K, Arakawa H, Buerstedde J-M, Sonoda E.
  • Journal Title: M o l C e l l B i o l. 28 Pages: 6113-22
  • Peer Reviewed
• [Journal Article] Single stranded DNA binding protein hSSB1 is critical for genomic stability. (2008)
  • Author(s): Richard D, Bolderson E, Cubeddu L, Wadsworth RIM, Savage K, Sharma GG, Nicolette ML, Tsvetanov S, McIlwraith MJ, Pandita R, Takeda S, West SC, Paull TT, Pandita1 TK, White MF, Hay TT, Gautier J, Khanna KK.
  • Journal Title: N a t u r e 453 Pages: 677-81
  • Peer Reviewed
• [Journal Article] DNA damage response protein ASCIZ links base excision repair with immunoglobulin gene conversion. (2008)
  • Author(s): Oka H, Sakai W, Sonoda E, Nakamura J, Asagoshi K, Wilson SH, Kobayashi M, Yamamoto K, Heierhorst J, Takeda S, Taniguchi Y.
  • Journal Title: B i o c h e m B i o p h y s R e s C o m m u n. 371 Pages: 225-9
  • Peer Reviewed
• [Journal Article] Ctp1/CtIP and the MRN Taniguchi Y, complex collaborate in the initial steps of homologous recombination. (2007)
  • Author(s): Takeda S, Nakamura K, Paull TT.
  • Journal Title: M o l C e l l. 28(Review) Pages: 351-2
  • Peer Reviewed
• [Journal Article] Connecting the Dots between Septins and the DNA Damage Checkpoint. (2007)
  • Author(s): Kinoshita M, Takeda S.
  • Journal Title: C e l l 130(Review) Pages: 777-9
  • Peer Reviewed
• [Journal Article] RAD51 up-regulation bypasses BRCA1 function Bishop DK. and is a common feature of BRCA1-deficient breast tumors. (2007)
  • Author(s): Martin RW, Orelli BJ, Yamazoe M, Minn AJ, Takeda S
  • Journal Title: C a n c e r R e s. 67 Pages: 9658-65
  • Peer Reviewed
• [Journal Article] Functional interactions between BLM and XRCC3 in the cell. (2007)
  • Author(s): Otsuki M, Seki M, Inoue E, Yoshimura A, Kato G, Yamanouchi S, Kawabe Y, Tada S, Shinohara A, Komura J, Ono T, Takeda S, Ishii Y, Enomoto T.
  • Journal Title: J C e l l B i o l. 179 Pages: 53-63
• [Journal Article] Inhibitors of the proteasome suppress homologous DNA recombination in mammalian cells. (2007)
  • Author(s): Murakawa Y, Sonoda E, Barber LJ, Zeng W, Yokomori K, Kimura H, Niimi A, Lehmann AR, Zhao GY, Hochegger H, Boulton SJ, Takeda S.
  • Journal Title: C a n c e r R e s. 67 Pages: 8536-8543
  • Peer Reviewed
• [Journal Article] An essential role for Cdk1 in S phase control, revealed by chemical genetics in vertebrate cells. (2007)
  • Author(s): Hochegger H, Dejsuphong D, Sonoda E, Saberi A, Rajendra E, Kirk J, Hunt T, Takeda S.
  • Journal Title: J C e l l B i o l. 178 Pages: 257-68
  • Peer Reviewed
• [Journal Article] Poly (ADP-ribose) Takeda S, Polymerase-1 Accelerates Single-Strand Break Repair in Concert with Poly (ADP-ribose) Glycohydrolase. (2007)
  • Author(s): Fisher A, Hochegger H, Caldecott KW.
  • Journal Title: M o l C e l l B i o l. 27 Pages: 5597-5605
  • Peer Reviewed
• [Journal Article] RAD18and poly[ADPribose]polymerase independently suppress the access of nonhomologous end joining to double strand breaks and facilitate homologous recombination mediated repair. (2007)
  • Author(s): Saberi A, Hochegger H, Szuts D, Lan L, Yasui A, Sale JE, Taniguchi Y, Y, Hua W, Yokomori K, Helleday T, Teraoka Murakawa H, Arakawa H, Buerstedde JM, Takeda S.
  • Journal Title: M o l C e l l B i o l. 27 Pages: 2562-2571
  • Peer Reviewed
• [Journal Article] Cooperative Roles of Vertebrate Fbh1 and Blm DNA Helicases in Avoidance of Crossovers during Recombination Initiated by Replication Fork Collapse. (2007)
  • Author(s): Kohzaki M, Hatanaka A, Sonoda E, Yamazoe M, Kikuchi K, Trung NV, Szuts D, Sale JE, Shinagawa H, Watanabe M, Takeda S.
  • Journal Title: M o l C e l l B i o l. 27 Pages: 2812-2820
  • Peer Reviewed
• [Journal Article] A critical role for the ubiquitin-conjugating enzyme Ubc13 in initiating homologous recombination. (2007)
  • Author(s): Zhao GY, Sonoda E, Barber LJ, Oka H, Murakawa Y, Yamada K, Ikura T, Wang X, Kobayashi M, Yamamoto K, Boulton SJ, Takeda S.
  • Journal Title: M o l C e l l. 25 Pages: 663-675
  • Peer Reviewed
• [Journal Article] Generation of medaka gene knockout models by target-selected mutagenesis. (2006)
  • Author(s): Taniguchi Y, Takeda S, M, Kamei Y, Todo T, Sasado T, Deguchi T, Furutani-Seiki Kondoh H, Mudde J, Yamazoe M, Hidaka M, Mitani H, Toyoda A, Sakaki Y, Plasterk RH, Cuppen E.
  • Journal Title: G e n o m e B i o l. 7(12) Pages: R116
  • Peer Reviewed
• [Journal Article] Vertebrate POLQ and POL cooperate in Base Excision Repair ofβOxidative DNA Damage. (2006)
  • Author(s): Yoshimura A, Kohzaki M, Nakamura J, Asagoshi K, Sonoda E, Hou E, Prasad R, Wilson SH, Tano K, Yasui A, Lan L, Seki M, Wood RD, Arakawa H, Buerstedde JM, Hochegger H, Okada T, Hiraoka M, Takeda S.
  • Journal Title: M o l C e l l. 24 Pages: 115-25
  • Peer Reviewed
• [Journal Article] Ubiquitin-Binding Motifs in REV1 Protein Are Required For Its Role in the Tolerance of DNA Damage. (2006)
  • Author(s): Guo C, Tang TS, Bienko M, Parker JL, Bielen AB, Sonoda E, Takeda S, Dikic I, Ulrich HD, Friedberg EC.
  • Journal Title: M o l C e l l B i o l. 26 Pages: 8892-900
  • Peer Reviewed
• [Journal Article] end-joining and homologous Differential usage of non-homologous recombination in double strand break repair. (2006)
  • Author(s): Sonoda E, Hochegger H, Saberi A, Taniguchi Y, Takeda S.
  • Journal Title: D N A R e p a i r ( A m s t ) 5(Review) Pages: 1021-9
  • Peer Reviewed
• [Journal Article] Interacts with PCNA: Significance of the REV1 BRCT Domain In Vitro and In REV1 Protein Vivo. (2006)
  • Author(s): Guo C, Sonoda E, Tang TS, Parker JL, Bielen AB, Takeda S, Ulrich HD, Friedberg EC.
  • Journal Title: M o l C e l l. 23 Pages: 265-71
  • Peer Reviewed
• [Journal Article] Genetic dissection of vertebrate 53BP1: a major role in non-homologous end joining of DNA double strand breaks. (2006)
  • Author(s): akamura K, Sakai W, Kawamoto T, Bree RT, Lowndes NF, Takeda S, Taniguchi Y.
  • Journal Title: D N A R e p a i r ( A m s t ) 5 Pages: 741-749
  • Peer Reviewed
• [Journal Article] Critical roles for Polζin Cellular Tolerance to Yamazoe M. Nitric Oxide induced DNA Damage. (2006)
  • Author(s): Xiaohua W, Takenaka K, Sonoda E, Hochegger H, Kawanishi S, Kawamoto T, Takeda S
  • Journal Title: C a n c e r R e s. 66 Pages: 748-54
  • Peer Reviewed
• [Journal Article] Multiple repair pathways mediate tolerance to chemotherapeutic cross-linking agents in vertebrate cells. (2005)
  • Author(s): Nojima K, Hochegger H, Saberi A, Fukushima T, Kikuchi K, Yoshimura M, Orelli BJ, Bishop DK, Hirano S, Ohzeki M, Ishiai M, Yamamoto K, Takata M, Arakawa H, Buerstedde JM, Yamazoe M, Kawamoto T, Araki K, Takahashi JA, Hashimoto N, Takeda S, Sonoda E.
  • Journal Title: C a n c e r R e s. 65 Pages: 11704-11
  • Peer Reviewed
• [Journal Article] Rad51 paralog deficiency on Similar effects of Brca2 truncation and immunoglobulin V gene diversification in DT40 cells support an early role for Rad51 paralogs in homologous recombination. (2005)
  • Author(s): Hatanaka A, Yamazoe M, Sale J.E., Takata M, Yamamoto K, Kitao H, Sonoda E, Kikuchi K, Yonetani Y, Takeda S.
  • Journal Title: M o l C e l l B i o l. 25 Pages: 1124-34
  • Peer Reviewed
• [Journal Article] in Homologous DNA Recombination Dual Roles for DNA Polymerase h and Translesion DNA Synthesis. (2005)
  • Author(s): Kawamoto T, Araki K, Sonoda E, Yamashita, Y.M., Harada, K.K., Kikuchi1, K., Masutani, C., Hanaoka, F., Nozaki, K., Hashimoto, N., Takeda, S.
  • Journal Title: M o l C e l l. 20 Pages: 793-9
  • Peer Reviewed
• [Presentation] Interactions between the RNF8 and RAD18 " Differential Functional E3 ubiquitin ligases in Translesion DNA Synthesis and DSB Repair " (2010)
  • Author(s): Takeda S
  • Organizer: EMBO-Recombination Mechanism
  • Place of Presentation: Il Ciocco, Italy(発表予定だったが、直前に家族の事情によりキャンセル)
  • Year and Date: 20100517-20100520
• [Presentation] Detect Environmental Mutagens and to "Chemical Genetic Method to Screen Anti-Malignant Chemical Compounds " (2009)
  • Author(s): Takeda S
  • Organizer: The 40th International Symposium of the Princess Takamatsu Cancer Research Fund
  • Place of Presentation: Hotel Grand Palace, Tokyo
  • Year and Date: 20091110-20091112
• [Presentation] DNA polymerases in TLS" Gordon Research "Reverse Genetic Analysis of Conference (2009)
  • Author(s): Takeda S
  • Organizer: Colby-Sawyer College, New London
  • Place of Presentation: New Hampshire, USA
  • Year and Date: 20090809-20090814
• [Presentation] RAD51 mediators, BRCA1, BRCA2, RAD51 "Reverse Genetic Study of paralogs, RAD52, Sws1, and Sfr1" (2009)
  • Author(s): Takeda S
  • Organizer: FASEB Genetic Recombination and Genome Rerrangements
  • Place of Presentation: Snowmass Village, Colorado, USA
  • Year and Date: 20090802-20090807
• [Presentation] repair-deficient chicken DT40 cell "A novel approach using DNA lines for screening and characterizing the genotoxicity of chemical compounds and environmental contaminants" (2009)
  • Author(s): Takeda S
  • Organizer: Chromosome Dynamics and Genome Stability, Villars sur Ollon
  • Place of Presentation: Switzerland
  • Year and Date: 20090514-20090516
• [Presentation] response by ubiquitin ligases " DNA (2009)
  • Author(s): Takeda S
  • Organizer: Regulation of DNA damage Repair
  • Place of Presentation: Crete, Greece
  • Year and Date: 20090420-20090423
• [Presentation] Ubiquitin-Conjugating Enzymes Ubc13 and " A Critical Role for the RNF8 in Homologous Recombination " (2009)
  • Author(s): Takeda S
  • Organizer: Gordon Research Conference
  • Place of Presentation: Ventura, USA
  • Year and Date: 20090208-20090213