2009 Fiscal Year Final Research Report
Functional analysis of the C-treminal frangment of membrane-anchored growth factors
| Project/Area Number |
17014068
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| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | Ehime University |
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Principal Investigator |
HIGASHIYAMA Shigeki Ehime University, 大学院・医学系研究科, 教授 (60202272)
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| Co-Investigator(Kenkyū-buntansha) |
MORIMOTO Chie 安田女子大学, 薬学部, 講師 (10332826)
HIEDA Miki 大阪大学, 大学院・医学系研究科, 特任助教 (00380254)
INOUE Hirofumi 愛媛大学, 大学院・医学系研究科, 講師 (70321635)
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| Project Period (FY) |
2005 – 2009
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| Keywords | シグナル伝達と遺伝子発現 / 膜型増殖因子 / shedding / CTFシグナル / 転写リプレッサー / レトログレード小胞輸送 |
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| Research Abstract |
We analyzed the function of C-terminal fragment (CTF) signals which are evoked by the shedding of a representative membrane-anchored growth factor proHB-EGF. We found that (1) the CTF of proHB-EGF was internalized into cytosol by endocytosis and localized at the innernuclear membrane by retrograde vesicle transport, (2) the CTF regulated several gene expression essential for cell growth promotion by interacting with and regulating gene repressors, and (3) the CTF signal was constitutively active in some tumor cell lines.
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