Cellular responses against DNA damage and search for novel molecular target

2009 Fiscal Year Final Research Report

• Project/Area Number: 17016075
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: University of Occupational and Environmental Health, Japan
• Principal Investigator: KOHNO Kimitoshi University of Occupational and Environmental Health, Japan, 医学部, 教授 (00153479)
• Project Period (FY): 2005 – 2009
• Keywords: DNA損傷 / 遺伝子発現制御 / YB-1 / ZNF143 / Clock / Tip60 / Wnt10A / シスプラチン

Research Abstract

Based on the analysis of drug resistant cells, we found several molecular targets such as YB-1, ZNF143, Clock and so on. These molecules are useful for cancer treatment. Molecular clocks in peripheral tissues control cell-proliferation rhythms by regulating the expression of cell-cycle genes. We also found the novel Wnt signaling pathway by which circadian disruption can induce malignant progression of tumor through angio/stromagenesis.

Research Products

• [Journal Article] Programmed cell death protein 4 down-regulates Y-box binding protein-1 expression via a direct interaction with Twist1 to suppress cancer cell growth. (2009)
  • Author(s): Shiota, M., Izumi, H., Tanimoto, A., Takahashi, M., Miyamoto, N., Kashiwagi, E., Kidani, A., Hirano, G., Masubuchi, D., Fukunaka, Y., Yasuniwa, Y., Naito, S., Nishizawa, S., Sasaguri, Y., Kohno, K.
  • Journal Title: Cancer Res. 69(7) Pages: 3148-3156
  • Peer Reviewed
• [Journal Article] Twist and p53 reciprocally regulate target genes via direct interaction. (2008)
  • Author(s): Shiota, M., Izumi, H., Onitsuka, T., Miyamoto, N., Kashiwagi, E., Kidani, A., Hirano, G., Takahashi, M., Naito, S., Kohno, K.
  • Journal Title: Oncogene 27(42) Pages: 5543-5553
  • Peer Reviewed
• [Journal Article] Tip60 is regulated by circadian transcription factor clock and is involved in cisplatin resistance. (2008)
  • Author(s): Miyamoto, N., Izumi, H., Noguchi, T., Nakajima, Y., Ohmiya, Y., Shiota, M., Kidani, A., Tawara, A., Kohno, K.
  • Journal Title: J. Biol. Chem. 283(26) Pages: 18218-18226
  • Peer Reviewed
• [Journal Article] Twist promotes tumor cell growth through YB-1 expression. (2008)
  • Author(s): Shiota, M., Izumi, H., Onitsuka, T., Miyamoto, N., Kashiwagi, E., Kidani, A., Yokomizo, A., Naito, S., Kohno, K.
  • Journal Title: Cancer Res. 68(1) Pages: 98-105
  • Peer Reviewed
• [Journal Article] ZNF143 interacts with p73 and is involved in cisplatin resistance through the transcriptional regulation of DNA repair genes. (2007)
  • Author(s): Wakasugi, T., Izumi, H., Uchiumi, T., Suzuki, H., Arao, T., Nishio, K., Kohno, K.
  • Journal Title: Oncogene 26(36) Pages: 5194-5203
  • Peer Reviewed
• [Journal Article] Clock and ATF4 transcription system regulates drug resistance in human cancer cell lines. (2007)
  • Author(s): Igarashi, T., Izumi, H., Uchiumi, T., Nishio, K., Arao, T., Tanabe, M., Uramoto, H., Sugio, K., Yasumoto, K., Sasaguri, Y., Wang, K.Y., Otsuji, Y., Kohno, K.
  • Journal Title: Oncogene 26(33) Pages: 4749-4760
  • Peer Reviewed
• [Journal Article] YB-1 is important for an early stage embryonic development: neural tube formation and cell proliferation. (2006)
  • Author(s): Uchiumi, T., Fotovati, A., Sasaguri, T., Shibahara, K., Shimada, T., Fukuda, T., Nakamura, T., Izumi, H., Tsuzuki, T., Kuwano, M., Kohno, K.
  • Journal Title: J. Biol. Chem. 281(52) Pages: 40440-40449
  • Peer Reviewed
• [Journal Article] Transcription factors and drug resistance. (2005)
  • Author(s): Kohno, K., Uchiumi, T., Niina, I., Wakasugi, T., Igarashi, T., Momii, Y., Yoshida, T., Matsuo, K., Miyamoto, N., Izumi, H.
  • Journal Title: Eur. J. Cancer 41(16) Pages: 2577-2586
  • Peer Reviewed
• [Journal Article] Roles of volume-sensitive Cl- channel in cisplatin-induced apoptosis in human epidermoid cancer cells. (2005)
  • Author(s): Ise, T., Shimizu, T., Lee, E.L., Inoue, H., Kohno, K., Okada, Y.
  • Journal Title: J. Membr. Biol. 205(3) Pages: 139-145
  • Peer Reviewed
• [Presentation] 概日リズムに制御されるがん細胞増殖と薬剤耐性. (2009)
  • Author(s): 河野公俊, 和泉弘人
  • Organizer: 第32回日本分子生物学会年会(ワークショップ)
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-12
• [Presentation] MCT4 expression and tumor invasion. (2009)
  • Author(s): Kohno, K.
  • Organizer: First International Symposium on Proton Transport and its Inhibition (PTI) in the etiopathogenesis, diagnosis and treatment of cancer (招待講演)
  • Place of Presentation: Madrid, Spain
  • Year and Date: 2009-04-28
• [Presentation] 抗がん剤耐性の分子機構:基礎研究から治療研究に向けて. (2008)
  • Author(s): 河野公俊
  • Organizer: 第60回日本泌尿器科学会西日本総会(招請講演)
  • Place of Presentation: 北九州
  • Year and Date: 2008-11-07
• [Presentation] Cisplatin resistance and transcription factors. (2007)
  • Author(s): Kohno, K., Izumi, H.
  • Organizer: The 12th Japanese Foundation for Cancer Research International Symposium on Cancer Chemotherapy (招待講演)
  • Place of Presentation: 東京
  • Year and Date: 2007-12-04
• [Presentation] 転写因子と抗がん剤耐性. (2007)
  • Author(s): 河野公俊
  • Organizer: 第16回泌尿器科分子・細胞研究会(特別講演)
  • Place of Presentation: 福岡
  • Year and Date: 2007-02-24
• [Presentation] 抗がん剤耐性の分子生物学からのアプローチ. (2006)
  • Author(s): 河野公俊
  • Organizer: 第24回日本口腔腫瘍学会総会・学術大会(特別講演)
  • Place of Presentation: 福岡
  • Year and Date: 2006-01-27
• [Presentation] Transcription factors and cisplatin resistance. (2005)
  • Author(s): Kohno, K
  • Organizer: The Japan Society for Promotion of Science (JSPS) U.S. -Japan Cooperative Cancer Research Program Seminar (招待講演)
  • Place of Presentation: 大阪
  • Year and Date: 2005-06-11
• [Book] がん化学療法・分子標的治療update (2009)
  • Author(s): 河野公俊, 和泉弘人
  • Total Pages: 64-67
  • Publisher: 中外医学社
• [Remarks]
  • URL: http://www.uoeh-u.ac.jp/kouza/bunsei/intro_j.html
• [Patent(Industrial Property Rights)] 胚細胞腫瘍の検出方法 (2009)
  • Inventor(s): 河野公俊, 笹栗靖之
  • Industrial Property Rights Holder: 株式会社キューリン
  • Industrial Property Number: 特許・特許第4395004号
  • Acquisition Date: 2009-10-23
• [Patent(Industrial Property Rights)] 予後判定方法 (2009)
  • Inventor(s): 松尾憲一, 山田雄次, 河野公俊
  • Industrial Property Rights Holder: 大鵬薬品工業株式会社
  • Industrial Property Number: 特許・特許第4253094号
  • Acquisition Date: 2009-01-30
• [Patent(Industrial Property Rights)] 癌細胞増殖阻害方法、増殖阻害剤及びスクリーニング方法 (2008)
  • Inventor(s): 河野公俊, 和泉弘人, 笹栗靖之
  • Industrial Property Rights Holder: 産業医科大学
  • Industrial Property Number: 特許・PCT/JP2008/073817
  • Filing Date: 2008-12-26