Quantitation analysis of pathway network analysis

2009 Fiscal Year Final Research Report

• Project/Area Number: 17017042
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: NATSUME Tohru National Institute of Advanced Industrial Science and Technology, バイオメディシナル情報研究センター, 研究チーム長 (00357683)
• Co-Investigator(Kenkyū-buntansha): IEMURA Shun-ichiro 独立行政法人産業技術総合研究所, バイオメディシナル情報研究センター, 主任研究員 (90356410) ; NAKAYAMA hiroshi 独立行政法人理化学研究所, 中央研究所, 研究員 (80321793) ; SHIBUYA Kohji 東京医科歯科大学, 難治疾患研究所, 教授 (30261324)
• Project Period (FY): 2005 – 2009
• Keywords: タンパク質間相互作 / タンパク質ネットワーク解析 / プロテオミクス / 質量分析 / 動態解析 / マップキナーゼカスケー / シグナル伝達 / Erk

Research Abstract

Cellular signaling comprises numerous numbers of proteins, which interact each other, Forming protein interaction network. To understand networks systematic andcomprehensively, we developed proteomic analytical platform to quantify proteininteractions. By the system, we found several novel signaling pathways.

Research Products

• [Journal Article] The selective autophagy substrate p62 activates the stress responsive transcription factor Nrf2 through inactivation of Keap1 (2010)
  • Author(s): Komatsu, M., H. Kurokawa, S. Waguri, K. Taguchi, A. Kobayashi, Y. Ichimura, Y.S. Sou, I. Ueno, A. Sakamoto, K.I. Tong, M. Kim, Y. Nishito, S.I. Iemura, T. Natsume, T. Ueno, E. Kominami, H. Motohashi, K. Tanaka, M. Yamamoto
  • Journal Title: Nat cell biology 12(3) Pages: 213-223
  • Peer Reviewed
• [Journal Article] Assembly pathway of the Mammalian proteasome base subcomplex is mediated by multiple specific chaperones (2009)
  • Author(s): Kaneko, T., J. Hamazaki, S. Iemura, K. Sasaki, K. Furuyama, T. Natsume, K. Tanaka, S. Murata
  • Journal Title: Cell 137(5) Pages: 914-925
  • Peer Reviewed
• [Journal Article] CHD8 suppresses p53-mediated apoptosis through histone H1 recruitment during early embryogenesis (2009)
  • Author(s): Nishiyama, M., K. Oshikawa, Y. Tsukada, T. Nakagawa, S. Iemura, T. Natsume, Y. Fan, A. Kikuchi, A.I. Skoultchi, K.I. Nakayama
  • Journal Title: Nat cell biology 11(2) Pages: 863-876
  • Peer Reviewed
• [Journal Article] FIP200, a ULK-interacting protein, is required for autophagosome formation in mammalian cells (2008)
  • Author(s): Hara, T., A. Takamura, C. Kishi, S. Iemura, T. Natsume, J.L. Guan, N. Mizushima
  • Journal Title: The Journal of cell biology 181(3) Pages: 497-510
  • Peer Reviewed
• [Journal Article] Activity-dependent synaptogenesis: regulation by a CaM-kinase kinase/CaM-kinase I/betaPIX signaling complex (2008)
  • Author(s): Saneyoshi, T., G. Wayman, D. Fortin, M. Davare, N. Hoshi, N. Nozaki, T. Natsume, T.R. Soderling
  • Journal Title: Neuron 57(1) Pages: 94-107
  • Peer Reviewed
• [Journal Article] Wnt signalling regulates paxillin ubiquitination essential for mesodermal cell motility (2007)
  • Author(s): Iioka, H., S. Iemura, T. Natsume, N. Kinoshita
  • Journal Title: Nat cell biology 9(7) Pages: 813-821
  • Peer Reviewed
• [Journal Article] Homeostatic levels of p62 control cytoplasmic inclusion body formation in autophagy-deficient mice (2007)
  • Author(s): Komatsu, M., S. Waguri, M. Koike, Y.S. Sou, T. Ueno, T. Hara, N. Mizushima, J. Iwata, J. Ezaki, S. Murata, J. Hamazaki, Y. Nishito, S. Iemura, T. Natsume, T. Yanagawa, J. Uwayama, E. Warabi, H. Yoshida, T. Ishii, A. Kobayashi, M. Yamamoto, Z. Yue, Y. Uchiyama, E. Kominami, K. Tanaka
  • Journal Title: Cell 131(6) Pages: 1149-1163
  • Peer Reviewed
• [Journal Article] XRab40 and XCullin5 form a ubiquitin ligase complex essential for the noncanonical Wnt pathway (2007)
  • Author(s): Lee, R.H., H. Iioka, M. Ohashi, S. Iemura, T. Natsume, N. Kinoshita
  • Journal Title: The EMBO journal 26(15) Pages: 3592-3606
  • Peer Reviewed
• [Journal Article] A novel proteasome interacting protein recruits the deubiquitinating enzyme UCH37 to 26S proteasomes (2006)
  • Author(s): Hamazaki, J., Iemura, S., Natsume, T., Yashiroda, H., Tanaka, K., Murata, S.
  • Journal Title: The EMBO journal 25(19) Pages: 4524-36
  • Peer Reviewed
• [Journal Article] XGAP, an ArfGAP, is required for polarized localization of PAR proteins and cell polarity in Xenopus gastrulation (2006)
  • Author(s): Hyodo-Miura, J., Yamamoto, T.S., Hyodo, A.C., Iemura, S., Kusakabe, M., Nishida, E., Natsume, T., Ueno, N.
  • Journal Title: Dev Cell 11(1) Pages: 69-79
  • Peer Reviewed
• [Journal Article] Shugoshin collaborates with protein phosphatase 2A to protect cohesin (2006)
  • Author(s): Kitajima, T.S., Sakuno, T., Ishiguro, K., Iemura, S., Natsume, T., Kawashima, S.A., Watanabe, Y.
  • Journal Title: Nature 441(7089) Pages: 46-52
  • Peer Reviewed
• [Journal Article] A novel ubiquitin-binding protein ZNF216 functioning in muscle atrophy (2006)
  • Author(s): Hishiya, A., Iemura, S., Natsume, T., Takayama, S., Ikeda, K., Watanabe, K.
  • Journal Title: Embo J 25(3) Pages: 554-64
  • Peer Reviewed
• [Presentation] New generation proteomics (2009)
  • Author(s): 夏目徹
  • Organizer: 日本ヒトプロテオーム機構
  • Place of Presentation: 北里大学
  • Year and Date: 2009-07-30
• [Presentation] Systematic Analysis of Protein Interaction Networks Using Human Full Length cDNA. (2005)
  • Author(s): 夏目徹
  • Organizer: International chemical congress of pacific basin societies
  • Place of Presentation: Hilton Hawaiian Village
  • Year and Date: 2005-12-16
• [Presentation] Systematic analysis of protein interaction network using human full length cDNA (2005)
  • Author(s): 夏目徹
  • Organizer: VIth European Symposium of The Protein Society
  • Place of Presentation: Barcelona
  • Year and Date: 2005-05-01
• [Book] 明日を拓く新次元プロテオミクス超々高感度質量分析への道のり (2009)
  • Author(s): 夏目徹
  • Total Pages: 61-68
  • Publisher: 学研メディカル秀潤社
• [Book] ストゲノム時代のタンパク質科学 (2006)
  • Author(s): 夏目徹
  • Publisher: 化学同人
• [Book] ゲノムから生命システムへ (2005)
  • Author(s): 夏目徹
  • Total Pages: 2065-71
  • Publisher: 共立出版