Functional studies and biogenesis of RNA modifications

2009 Fiscal Year Final Research Report

• Project/Area Number: 17018008
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: The University of Tokyo
• Principal Investigator: SUZUKI Tsutomu The University of Tokyo, 大学院・工学系研究科, 教授 (20292782)
• Project Period (FY): 2005 – 2009
• Keywords: RNA修飾 / リボヌクレオーム解析 / RNAマススペクトロメトリー / RNAエディティング / イノシン / ライシジン / ワイブトシン / チオウリジン / アセチルシチジン

Research Abstract

This project aimed to study on functional aspects and biogenesis of post-transcriptional modifications of RNA molecules associated with various biological processes. We employed a systematic genome-wide screen of genes responsible for biogenesis of RNA modifications using RNA mass spectrometry combined with reverse genetic approach. We identified novel genes responsible for synthesizing 2-thiouridine, wybutosine, lysidine and N4-acetylcytidine that are found in tRNAs from E. coli or S. cerevisiae. For identification of inosines in mRNAs from human brain, we have established a biochemical method, named "inosine chemical erasing (ICE)", to directly detect inosines on RNA strands. We have mapped more than 16,000 novel sites of inosines in human brain transcriptome. Furthermore, we have started a project of genome-wide identification of inosine sites by using the ICE-method combined with the deep sequencing (ICE-Seq).

Research Products

• [Journal Article] Discovery and characterization of tRNAIle lysidine synthetase (2010)
  • Author(s): Suzuki, T. *, Miyauchi, K.
  • Journal Title: FEBS Lett 584 Pages: 272-277
• [Journal Article] Fine-tuning of the ribosomal decoding center by conserved methyl-modifications in the Escherichia coli 16S rRNA (2010)
  • Author(s): Kimura, S., Suzuki, T.
  • Journal Title: Nucleic Acids Res 38 Pages: 1341-1352
• [Journal Article] The TDRD9-MIWI2 complex is essential for piRNA-mediated retrotransposon silencing (2009)
  • Author(s): Shoji, M., Tanaka, T., Hosokawa, M., Reuter, M., Stark, A., Kato, Y., Kondoh, G., Okawa, K., Chujo, T., Suzuki, T., Hata, K., Martin, S.L., Noce, T., Kuramochi-Miyagawa, S., Nakano, T., Sasaki, H., Pillai, R.S., Nakatsuji, N., Chuma, S.
  • Journal Title: in the mouse male germline Germline Dev Cell 17 Pages: 775-787
• [Journal Article] Structural basis for translational fidelity ensured by tRNA (2009)
  • Author(s): Nakanishi, K., Bonnefond, L., Kimura, S., Suzuki, T., Ishitani, R., Nureki, O.
  • Journal Title: lysidine synthetase Nature 461 Pages: 1144-1148
• [Journal Article] 3D-structure of mammalian mitochondrial tRNAA (2009)
  • Author(s): Messmer, M., Putz, J., Suzuki, T., Suzuki, T., Sauter, C., Sissler, M., Florentz, C.
  • Journal Title: sp revealed by solution probing and phylogeny Nucleic Acids Res. 37 Pages: 6881-6895
• [Journal Article] Structural basis of AdoMet-dependent aminocarboxypropyl transfer reaction catalyzed by tRNA-wybutosine synthesizing enzyme (2009)
  • Author(s): Umitsu, M., Nishimasu, H., Noma, A., Suzuki, T., Ishitani, R., Nureki, O.
  • Journal Title: Proc. Natl. Acad. Sci. USA. 106 Pages: 15616-15621
• [Journal Article] Biogenesis of glutaminyl-tRNAGln in human mitochondria Proc (2009)
  • Author(s): Nagao, A., Suzuki, T., Katoh, T., Sakaguchi, Y., Suzuki, T. *
  • Journal Title: Natl. Acad. Sci. USA. 106 Pages: 16209-16214
• [Journal Article] The R336Q mutation in human mitochondrial EFTu prevents the formation of an active mt-EFTu・GTP・aa-tRNA ternary complex (2009)
  • Author(s): Valente, L., Shigi, N., Suzuki, T., Zeviani, M.
  • Journal Title: Biochim Biophys Acta. 1792 Pages: 791-795
• [Journal Article] Aquifex aeolicus tRNA (N2, N2-guanine)-dimethyltransferase (Trm1) catalyzes transfer of methyl groups not only to guanine 26 but also to guanine 27 in tRNA (2009)
  • Author(s): Awai, T., Kimura, S., Tomikawa, C., Ochi, A., Ihsanawati, Bessho, Y., Yokoyama, S., Ohno, S., Nishikawa, K., Yokogawa, T., Suzuki, T., Hori, H.
  • Journal Title: J. Biol. Chem. 284 Pages: 20467-20478
• [Journal Article] The ordered transcription of RNA (2009)
  • Author(s): Kitahara, K., Suzuki, T. *
  • Journal Title: domains is not essential for ribosome biogenesis Mol Cell. 34 Pages: 760-766
• [Journal Article] Structural basis of tRNA modification with CO2 fixation and methylation by wybutosine synthesizing enzyme TYW4 (2009)
  • Author(s): Suzuki, Y., Noma, A., Suzuki, T., Ishitani, R., Nureki, O.
  • Journal Title: Nucleic Acids Res. 37 Pages: 2910-2925
• [Journal Article] RNA helicase module in an acetyltransferase that modifies a specific tRNA (2009)
  • Author(s): Chimnaronk, S., Suzuki, T., Manita, T., Ikeuchi, Y., Yao, M., Suzuki, T., Tanaka, I.
  • Journal Title: anticodon EMBO J. 28 Pages: 1362-1373
• [Journal Article] Chemical synthesis and properties of 5-taurinomethyluridine and 5-taurinomethyl-2-thiouridine (2009)
  • Author(s): Ogata, T., Shimazaki, T., Umemoto, T., Kurata, S., Ohtsuki, T., Suzuki, T., Wada, T.
  • Journal Title: J. Org. Chem. 74 Pages: 2585-2588
• [Journal Article] Selective stabilization of mammalian microRNAs by 3'-adenylation mediated by the cytoplasmic poly(A) polymerase GLD-2 (2009)
  • Author(s): Katoh, T., Sakaguchi, Y., Miyauchi, K., Suzuki, T., Kashiwabara, S., Baba, T., Suzuki, T. *
  • Journal Title: Genes Dev. 23 Pages: 433-438
• [Journal Article] Biogenesis and functions of thio-compounds in transfer RNA: comparison of bacterial and eukaryotic thiolation machineries DNA and RNA modification enzymes (Book LANDES) (2009)
  • Author(s): Noma, A., Shigi, N., Suzuki, T. *
  • Journal Title: ed Grosjean, H.
• [Journal Article] Mechanistic characterization of the sulfur-relay system for eukaryotic 2-thiouridine biogenesis at tRNA wobble positions (2009)
  • Author(s): Noma, A., Sakaguchi, Y., Suzuki, T. *
  • Journal Title: Nucleic Acids Res. 37 Pages: 1335-1352
• [Journal Article] Precise analysis of modification status at various stage of tRNA maturation in Saccharomyces cerevisiae (2009)
  • Author(s): Ohira, T., Miyauchi, K., Sakaguchi, Y., Suzuki, T., Suzuki, T.
  • Journal Title: Nucleic Acids Symp Ser (Oxf) 53 Pages: 301-302
• [Journal Article] Common Sulfur Transfer System for the Biosyntheses of Sulfur-containing (2008)
  • Author(s): Shigi, N., Sakaguchi, Y., Asai, S., Suzuki, T., Watanabe, K.
  • Journal Title: tRNA and Cofactors EMBO J. 27 Pages: 3267-3278
• [Journal Article] The RNA acetyltransferase driven by ATP hydrolysis synthesizes (2008)
  • Author(s): Ikeuchi, Y., Kitahara, K., Suzuki, T. *
  • Journal Title: N4-acetylcytidine of tRNA anticodon EMBO J. 27 Pages: 2194-2203
• [Journal Article] Measuring mRNA decay in human mitochondria (2008)
  • Author(s): Nagao, A., Shigi-Hino, N., Suzuki, T. *
  • Journal Title: Methods in Enzymol. 447 Pages: 489-499
• [Journal Article] Ribosomal RNAs are tolerant toward genetic insertions: Evolutionary origin of the expansion segments (2008)
  • Author(s): Yokoyama, T., Suzuki, T. *
  • Journal Title: Nucleic Acids Res. 36 Pages: 3539-3551
• [Journal Article] Modified uridines with C5-methylene substituents at the first position of the tRNA anticodon stabilize U・G wobble pairing during decoding (2008)
  • Author(s): Kurata, S., Weixlbaumer, A., Ohtsuki, T., Shimazaki, T., Wada, T., Kirino, Y., Takai, K., Watanabe, K. Ramakrishnan, V., Suzuki, T. *
  • Journal Title: J. Biol. Chem. 283 Pages: 18801-18811
• [Journal Article] Polyadenylation in mammalian mitochondria: Insights from recent studies (2008)
  • Author(s): Nagaike, T., Suzuki, T., Ueda, T.
  • Journal Title: Biochim Biophys Acta 1779 Pages: 266-269
• [Journal Article] Mechanism of mRNA deadenylation: evidence for a molecular interplay between translation termination factor eRF3 and mRNA deadenylases (2007)
  • Author(s): Funakoshi, Y., Doi, Y., Hosoda, N., Uchida, N., Osawa, M., Shimada, I., Tsujimoto, M., Suzuki, T., Katada, T., Hoshino, S.
  • Journal Title: Genes Dev. 21 Pages: 3135-3148
• [Journal Article] Wobble inosine tRNA modification is essential for cell cycle progression in G1/S and G2/M transitions in fission yeast (2007)
  • Author(s): Tsutsumi, S., Sugiura, R., Ma, Y., Tokuoka, H., Ohta, K., Ohte, R., Noma, A., Suzuki, T., Kuno, T.
  • Journal Title: J. Biol. Chem. 282 Pages: 33459-33465
• [Journal Article] O Crystal structure of the radical SAM enzyme catalyzing tricyclic modified base formation in tRNA (2007)
  • Author(s): Saito, K., Sakaguchi, Y., Suzuki, T., Suzuki, T., Siomi, H., Siomi, M.C. Pimet
  • Journal Title: J. Mol. Biol. 372 Pages: 1204-1214
• [Journal Article] the Drosophila homolog of HEN1, mediates 2'-O-methylation of Piwi-interacting RNAs at their 3' ends (2007)
  • Author(s): Saito, K., Sakaguchi, Y., Suzuki, T., Suzuki, T., Siomi, H., Siomi, M.C. Pimet
  • Journal Title: Genes Dev. 21 Pages: 1603-1608
• [Journal Article] Functional genetic selection of Helix 66 in Escherichia coli 23S rRNA identified the eukaryotic class of binding sequences for ribosomal protein L2 (2007)
  • Author(s): Kitahara, K., Kajiura, A., Sato, N.S., Suzuki, T. *
  • Journal Title: Nucleic Acids Res. 35 Pages: 4018-4029
• [Journal Article] Mass spectrometric analysis of 3'-terminal nucleosides in non-coding RNAs Nat Protoc. (2007)
  • Author(s): Suzuki, T., Sakaguchi, Y., Suzuki, T. *
  • Journal Title: DOI: 10. 1038/nprot 185
• [Journal Article] Structures of tRNAs with an expanded anticodon loop in the decoding center of the 30S (2007)
  • Author(s): Dunham, C.M., Selmer, M., Phelps, S.S., Suzuki, T., Joseph, S., Ramakrishnan, V.
  • Journal Title: Ribosomal Subunit RNA 13 Pages: 817-823
• [Journal Article] The 3'-termini of mouse piwi-interacting RNAs are 2'-O-methylated (2007)
  • Author(s): Ohara, T., Sakaguchi, Y., Suzuki, T., Ueda, H., Miyauchi, K., Suzuki, T. *
  • Journal Title: Nat Struct Mol Biol. 14 Pages: 349-350
• [Journal Article] Thio modification of yeast cytosolic tRNA is an iron-sulfur protein-dependent pathway (2007)
  • Author(s): Nakai, Y., Nakai, M., Lill, R., Suzuki, T., Hayashi, H.
  • Journal Title: Mol Cell Biol. 27 Pages: 2841-2847
• [Journal Article] Chaplet column chromatography: isolation of a large set of individual RNAs in a single step. (2007)
  • Author(s): Suzuki, T., Suzuki, T. *
  • Journal Title: Methods in Enzymol. 425 Pages: 231-239
• [Journal Article] Mass spectrometric identification and characterization of RNA-modifying enzymes. (2007)
  • Author(s): Suzuki, T. *, Ikeuchi, Y., Noma, A., Suzuki, T., Sakaguchi, Y.
  • Journal Title: Methods in Enzymol. 425 Pages: 211-229
• [Journal Article] Automated parallel isolation of multiple species of non-coding RNAs by the reciprocal circulating chromatography method (2007)
  • Author(s): Miyauchi, K., Ohara, T., Suzuki, T. *
  • Journal Title: Nucleic Acids Res. 35 Pages: e24
• [Journal Article] Specific residues at every third position of siRNA shape its efficient RNAi activity (2007)
  • Author(s): Katoh, T., Suzuki, T. *
  • Journal Title: Nucleic Acids Res. 35 Pages: e27
• [Journal Article] Ligand-induced translation by the allosteric ribosome bearing an aptamer-fused rRNA (2007)
  • Author(s): Yokoyama, T., Suzuki, T.
  • Journal Title: Nucleic Acids Symp Ser 51 Pages: 383-384
• [Journal Article] Aminoacyl-tRNA surveillance by EF-Tu in mammalian mitochondria (2007)
  • Author(s): Nagao, A., Suzuki, T., Suzuki, T.
  • Journal Title: Nucleic Acids Symp Ser 51 Pages: 41-42
• [Journal Article] Animated Crystallography of Genetic Code Translation (2007)
  • Author(s): Tomita, K., Numata, T., Fukai, T., Suzuki, T., Ishitani, R., Nureki, O.
  • Journal Title: Nucleic Acids Symp Ser 51 Pages: 101-102
• [Journal Article] The substrate specificity of tRNA (m1G37) methyltransferase (TrmD) from Aquifex aeolicus (2006)
  • Author(s): Takeda, H., Toyooka, T., Ikeuchi, Y., Yokobori, S., Okadome, K., Takano, F., Oshima, T., Suzuki, T., Endo, Y., Hori, H.
  • Journal Title: Genes Cells 11 Pages: 1353-1365
• [Journal Article] The A-site finger in 23S rRNA acts as a functional attenuator for translocation (2006)
  • Author(s): Komoda, T., Sato, N.S., Phelps, S.S., Namba, N., Joseph, S., Suzuki, T. *
  • Journal Title: J. Biol. Chem. 281 Pages: 32303-32309
• [Journal Article] Identification of the residues involved in the unique serine specificity of Caenorhabditis elegans mitochondrial EF-Tu2 (2006)
  • Author(s): Sato, A., Watanabe, Y., Suzuki, T., Komiyama, M., Watanabe, K., Ohtsuki, T.
  • Journal Title: Biochemistry 45 Pages: 10920-10927
• [Journal Article] Comprehensive genetic selection revealed essential bases in the peptidyl-transferase center (2006)
  • Author(s): Sato, N.S., Hirabayashi, N., Agmon, I., Yonath, A., Suzuki, T. *
  • Journal Title: Proc. Natl. Acad. Sci. USA. 103 Pages: 15386-15391
• [Journal Article] Snapshots of tRNA suluration via an adenylated intermediate (2006)
  • Author(s): Numata, T., Ikeuchi, Y., Fukai, S., Suzuki, T., Nureki, O.
  • Journal Title: Nature 442 Pages: 419-424
• [Journal Article] Mutation in TRMU related to mitochondrial tRNA modification modulates the phenotypic expression of the deafness-associated mitochondrial 12S rRNA mutations (2006)
  • Author(s): Guan, M.X., Yan, Q., Li, X., Bykhovskaya, Y., Gallo-Teran, J., Hajek, P., Umeda, N., Zhao, H., Garrido, G., Mengesha, E., Suzuki, T., del Castillo, I., Peters, J. L., Li, R., Qian, Y., Wang, X., Shohat, M., Estivill, X., Watanabe, K., Fischel-Ghodsian, N.
  • Journal Title: Am J Hum Genet. 79 Pages: 291-302
• [Journal Article] Conserved loop sequence of helix 69 in Escherichia coli 23S rRNA is involved in A-site tRNA binding and translational fidelity (2006)
  • Author(s): Hirabayashi, N., Sato, N.S., Suzuki, T. *
  • Journal Title: J. Biol. Chem. 281 Pages: 17203-17211
• [Journal Article] Crystallization and preliminary X-ray analysis of the tRNA thiolation enzyme MnmA from Escherichia coli complexed with tRNA(Glu) (2006)
  • Author(s): Numata, T., Ikeuchi, Y., Fukai, S., Adachi, H., Matsumura, H., Takano, K., Murakami, S., Inoue, T., Mori, Y., Sasaki, T., Suzuki, T., Nureki, O.
  • Journal Title: Acta Crystallograph Sect F Struct Biol Cryst Commun 62 Pages: 368-371
• [Journal Article] Biosynthesis of wybutosine, a hyper-modified nucleoside in eukaryotic phenylalanine tRNA (2006)
  • Author(s): Noma, A., Kirino, Y., Ikeuchi, Y., Suzuki, T. *
  • Journal Title: EMBO J 25 Pages: 2142-2154
• [Journal Article] Identification of two tRNA-thiolation genes required for cell growth at extremely high temperatures (2006)
  • Author(s): Shigi, N., Sakaguchi, Y., Suzuki, T., Watanabe, K.
  • Journal Title: J. Biol. Chem. 281 Pages: 14296-14306
• [Journal Article] Acquisition of the wobble modification in mitochondrial tRNALeu(CUN) bearing the G12300A mutation suppresses the MELAS molecular defect (2006)
  • Author(s): Kirino, Y., Yasukawa, T., Marjavaara, S.K., Jacobs, H.T., Holt, I.J., Watanabe, K., Suzuki, T. *
  • Journal Title: Hum Mol Genet. 15 Pages: 897-904
• [Journal Article] A gene involved in modifying transfer RNA is required for fungal pathogenicity and stress tolerance of Colletotrichum lagenarium (2006)
  • Author(s): Takano, Y., Takayanagi, N., Hori, H., Ikeuchi, Y., Suzuki, T., Kimura, A., Okuno, T.
  • Journal Title: Mol Microbiol 60 Pages: 81-92
• [Journal Article] Temperature-dependent biosynthesis of 2-thioribothymidine of Thermus thermophilus tRNA (2006)
  • Author(s): Shigi, N., Suzuki, T., Terada, T., Shirouzu, M., Yokoyama, S., Watanabe, K.
  • Journal Title: J. Biol. Chem. 281 Pages: 2104-2113
• [Journal Article] Structural basis for sulfur relay to RNA mediated by heterohexameric TusBCD complex (2006)
  • Author(s): Numata, T., Fukai, S., Ikeuchi, Y., Suzuki, T. *, Nureki*, O.
  • Journal Title: Structure 14 Pages: 357-366
• [Journal Article] Mechanistic insights into sulfur-relay by multiple sulfur mediators involved in thiouridine biosynthesis at tRNA wobble positions (2006)
  • Author(s): Ikeuchi, Y., Shigi, N. Kato, J., Nishimura, A., Suzuki, T. *
  • Journal Title: Mol Cell. 21 Pages: 97-108
• [Journal Article] Involvement of the Escherichia coli folate-binding protein YgfZ in RNA modification and regulation of chromosomal replication initiation (2005)
  • Author(s): Ote, T., Hashimoto, M., Ikeuchi, Y., Suetsugu, M., Suzuki, T., Katayama, T., Kato, J.
  • Journal Title: Mol Microbiol. 59 Pages: 265-275
• [Journal Article] Antibiotic susceptibility of mammalian mitochondrial translation (2005)
  • Author(s): Zhang, L., Ching Ging, N., Komoda, T., Hanada, T., Suzuki, T. *, Watanabe, K*.
  • Journal Title: FEBS Lett. 579 Pages: 6423-6427
• [Journal Article] Dual Mode Recognition of noncanonical tRNAsSer by seryl-tRNA synthetase in mammalian mitochondria (2005)
  • Author(s): Chimnaronk, S., Jeppesen, M.G., Suzuki, T., Nyborg, J., Watanabe, K.
  • Journal Title: EMBO J. 24 Pages: 3369-3379
• [Journal Article] Molecular mechanism of lysidine synthesis that determines tRNA identity and codon recognition (2005)
  • Author(s): Ikeuchi, Y., Soma, A., Ote, T., Kato, J., Sekine, Y., Suzuki, T. *
  • Journal Title: Mol Cell. 19 Pages: 235-246
• [Journal Article] Specific correlation between tRNA taurine-modification deficiency and clinical features of human mitochondrial disease Proc. (2005)
  • Author(s): Kirino, Y., Goto, Y., Campos, Y., Arenas, J., Suzuki, T. *
  • Journal Title: Natl. Acad. Sci. USA. 102 Pages: 7127-7132
• [Journal Article] Structural basis for lysidine formation by ATP pyrophosphatase accompanied with a lysine-specific loop and a tRNA-recognition domain Proc (2005)
  • Author(s): Nakanishi, K., Fukai, S., Ikeuchi, Y., Soma, A., Sekine, Y., Suzuki, T., Nureki, O.
  • Journal Title: Natl. Acad. Sci. USA. 102 Pages: 7487-7492
• [Journal Article] Wobble modification deficiency in mutant tRNAs in patients with mitochondrial diseases (2005)
  • Author(s): Yasukawa, T., Kirino, Y., Ishii, N., Holt, I.J., Jacobs, H.T., Makifuchi, T., Fukuhara, N., Ohta, S., Suzuki, T. *, Watanabe, K*.
  • Journal Title: FEBS Lett. 579 Pages: 2948-2952
• [Journal Article] Unusual usage of wobble modification in mitochondrial tRNAs of the nematode Ascaris suum (2005)
  • Author(s): Sakurai, M., Ohtsuki, T., Suzuki, T., Watanabe, K.
  • Journal Title: FEBS Lett 579 Pages: 2767-2772
• [Journal Article] Human mitochondrial mRNAs are stabilized with polyadenylation regulated by mitochondria-specific poly(A) polymerase and polynucleotide phosphorylase (2005)
  • Author(s): Nagaike, T., Suzuki, T. *., Katoh, T., Ueda, T.
  • Journal Title: J. Biol. Chem. 280 Pages: 19721-19727
• [Journal Article] Biosynthesis and function of tRNA wobble modifications (2005)
  • Author(s): Suzuki, T.
  • Journal Title: In Fine-tuning of RNA functions by modification and editing Topics in Curr Genetics vol12 Pages: 24-69
• [Journal Article] Mitochondria-specific RNA-modifying enzymes responsible for the biosynthesis of the wobble base in mitochondrial tRNAs: Implications for the molecular pathogenesis of human mitochondrial diseases (2005)
  • Author(s): Umeda, N., Suzuki, T., Yukawa, M., Ohya, Y., Shindo, H., Watanabe, K., Suzuki, T. *.
  • Journal Title: J. Biol. Chem 280 Pages: 1613-1624
• [Presentation] Biogenesis of Gln-mt tRNAGln in human mitochondria Asuteka Nagao (2010)
  • Author(s): Takeo Suzuki, Takayuki Katoh, Yuriko Sakaguchi, Tsutomu Suzuki
  • Organizer: The 23rd tRNA workshop
  • Place of Presentation: Aveiro
  • Year and Date: 20100128-20100202
• [Presentation] Novel wobble modification in tRNAIle responsible for decoding AUA codon in archaeal species; convergent evolution of the decoding system across domains of life Satoshi Kimura, Yoshiho (2010)
  • Author(s): Ikeuchi, Tomoyuki Numata, Daigo Nakamura, Takashi Yokogawa, Kazuya Nishikawa, Takeshi Wada, Takeo Suzuki, Tsutomu Suzuki
  • Organizer: The 23rd tRNA workshop
  • Place of Presentation: Aveiro
  • Year and Date: 20100128-20100202
• [Presentation] A landscape of tRNA modifications: complete chemical structures of total 48 species of Escherichia coli tRNAs determined by mass spectrometry (2010)
  • Author(s): Kenjyo Miyauchi, Yuriko Sakaguchi, Takeo Suzuki, Tsutomu Suzuki
  • Organizer: The 23rd tRNA workshop
  • Place of Presentation: Aveiro
  • Year and Date: 20100128-20100202
• [Presentation] Requirements for in vitro formation of 5-carboxymethylaminometyluridine at the wobble position in Escherichia coli tRNAs (2010)
  • Author(s): Takeo Suzuki, Tomoyuki Numata, Takuo Osawa, Tsutomu Suzuki
  • Organizer: The 23rd tRNA workshop
  • Place of Presentation: Aveiro
  • Year and Date: 20100128-20100202
• [Presentation] RNAの直接解析で見えてきたもの～microRNAが有する質的な情報と選択的安定化機構～ (2010)
  • Author(s): 鈴木勉
  • Organizer: 臨床応用を目指した最前線セミナー～microRNAを標的とした診断・治療の開発に向けて～
  • Place of Presentation: 東京
  • Year and Date: 2010-02-15
• [Presentation] A landscape of A-to-I RNA editing in human transcriptome: a hidden layer of gene expression produced by qualitative information embedded in RNA molecules (2009)
  • Author(s): Tsutomu Suzuki, Hiroki Ueda, Takanori Yano, Shunpei Okada, Hideki Terajima, Totai Mitsuyama, Atsushi Toyoda, Asao Fujiyama, Hitomi Kawabata, Masayuki Sakurai
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 20091209-20091212
• [Presentation] マススペクトロメトリーによるmiRNAの直接プロファイリングとmiRNAの特異的安定化機構の発見 (2009)
  • Author(s): 坂口裕理子, 加藤敬行, 上田宏生, 宮内健常, 柏原真一, 馬場忠, 姜秉一, 栗原靖之, 鈴木健夫, 鈴木勉
  • Organizer: 第82回日本生化学会年会
  • Place of Presentation: 神戸
  • Year and Date: 20091021-20091024
• [Presentation] Precise analysis of modification status at various stage of tRNA maturation in Saccharomyces cerevisiae (2009)
  • Author(s): Takayuki Ohira, Kenjyo Miyauchi, Yuriko Sakaguchi, Takeo Suzuki, Tsutomu Suzuki
  • Organizer: 国際核酸化学シンポジウム
  • Place of Presentation: 富山
  • Year and Date: 20090927-20091001
• [Presentation] の逆行性の輸送が塩基修飾の形成に関わる (2009)
  • Author(s): 大平高之、鈴木勉
  • Organizer: 第11回日本RNA学会年会
  • Place of Presentation: 新潟
  • Year and Date: 20090727-20090729
• [Presentation] RNAドメインの序列的な転写はリボソームの生合成に必須ではない (2009)
  • Author(s): 北原圭、鈴木勉
  • Organizer: 第11回日本RNA学会年会
  • Place of Presentation: 新潟
  • Year and Date: 20090727-20090729
• [Presentation] イントロン領域におけるA-to-I RNAエディティングの機能解析 (2009)
  • Author(s): 矢野孝紀、櫻井雅之、上田宏生、岡田俊平、川畑瞳、鈴木勉
  • Organizer: 第11回日本RNA学会年会
  • Place of Presentation: 新潟
  • Year and Date: 20090727-20090729
• [Presentation] 大腸菌RlmKLは23S rRNA中における2種類の異なるメチル化塩基の生合成を触媒する新しいタイプのRNA修飾酵素である (2009)
  • Author(s): Satoshi Kimura, Yoshiho Ikeuchi, Takeo Suzuki, Kei Kitahara, Yuriko Sakaguchi, Tsutomu Suzuki
  • Organizer: 第11回日本RNA学会年会
  • Place of Presentation: 新潟
  • Year and Date: 20090727-20090729
• [Presentation] 往復循環クロマトグラフィーを用いた微量RNAの全自動単離精製と修飾解析 (2009)
  • Author(s): 宮内健常, 坂口裕理子, 鈴木健夫, 鈴木勉
  • Organizer: 第11回日本RNA学会年会
  • Place of Presentation: 新潟
  • Year and Date: 20090727-20090729
• [Presentation] A-to-I RNA editingによるmiRNA依存的翻訳制御の調節 (2009)
  • Author(s): 矢野孝紀、櫻井雅之、上田宏生、岡田俊平、川畑瞳、鈴木勉
  • Organizer: 第11回日本RNA学会年会
  • Place of Presentation: 新潟
  • Year and Date: 20090727-20090729
• [Presentation] ICE法と次世代シーケンサーを組み合わせたヒト成人脳由来RNA中に含まれるイノシン化部位の網羅的探索 (2009)
  • Author(s): 上田宏生、櫻井雅之、川畑瞳、光山統泰、矢野孝紀、岡田俊平、豊田敦、藤山秋佐夫、鈴木勉
  • Organizer: 第11回日本RNA学会年会
  • Place of Presentation: 新潟
  • Year and Date: 20090727-20090729
• [Presentation] ヒト転写産物におけるRNAエディティング部位探索と機能解析 (2009)
  • Author(s): 櫻井雅之、川畑瞳、矢野孝紀、岡田俊平、光山統泰、豊田敦、藤山秋佐夫、上田宏生、鈴木勉
  • Organizer: 第11回日本RNA学会年会
  • Place of Presentation: 新潟
  • Year and Date: 20090727-20090729
• [Presentation] Large scale identification of A-to-I editing sites in the human brain transcriptome by the ICE method; implication for modulatory effect of A-to-I editing on translational repression mediated by miRNAs (2009)
  • Author(s): Masayuki Sakurai, Takanori Yano, Shunpei Okada, Hitomi Kawabata, Totai Mitsuyama, Hiroki Ueda, Tsutomu Suzuki
  • Organizer: Gordon Reseach Conference for RNA editing
  • Place of Presentation: Galveston
  • Year and Date: 20090112-20090116
• [Presentation] The acetylation of 18S rRNA is an essential modification to synthesize the small subunit of eukaryotic ribosome (2009)
  • Author(s): Satoshi Ito, Yu Akamatsu, Akiko Noma, Satoshi Kimura, Yoshiho Ikeuchi, Yoshikazu Tanaka, Kenjyo Miyauchi, Isao Tanaka, Takeo Suzuki, Tsutomu Suzuki
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-10
• [Presentation] Direct analysis of small non-coding RNAs by mass spectrometry (2009)
  • Author(s): Tsutomu Suzuki
  • Organizer: Joint Symposium of the 5th Annual Meeting of OTS and The 19th Antisense Symposium
  • Place of Presentation: 福岡
  • Year and Date: 2009-11-06
• [Presentation] トップランナーズレクチャーRNAの直接解析で見えてきたもの (2009)
  • Author(s): 鈴木勉
  • Organizer: 第82回日本生化学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-23
• [Presentation] RNAの直接解析で見えてきたもの～microRNAが有する質的な情報と選択的安定化機構～ (2009)
  • Author(s): 鈴木勉
  • Organizer: KASTセミナー, 機能性RNAコース～機能性RNAの歴史と応用展開～
  • Place of Presentation: 神奈川
  • Year and Date: 2009-09-16
• [Presentation] Circular permutants of the ribosomes in the cell (2009)
  • Author(s): Kei Kitahara, Tsutomu Suzuki
  • Organizer: Experimental biology 2009
  • Place of Presentation: New Orleans
  • Year and Date: 2009-04-18
• [Presentation] Mass spectrometric identification of non-coding RNAs in ribonucleoprotein complexes in yeast (2008)
  • Author(s): Takayuki Ohira, Yuki Takeuchi, Yuriko Sakaguchi, Hiroki Ueda, Takeo Suzuki, Tsutomu Suzuki
  • Organizer: 13th annual meeting of the RNA society (RNA2008)
  • Place of Presentation: Berlin
  • Year and Date: 20080728-20080803
• [Presentation] 酵母tRNAウォブル位のチオウリジン修飾はユビキチン様の反応機構を経由する (2008)
  • Author(s): 野間章子、鈴木勉
  • Organizer: 第10回日本RNA学会年会
  • Place of Presentation: 札幌
  • Year and Date: 20080723-20080725
• [Presentation] 往復循環クロマトグラフィー法によるnon-coding RNAの全自動単離精製 (2008)
  • Author(s): 宮内健常, 坂口裕理子, 鈴木健夫, 鈴木勉
  • Organizer: 第10回日本RNA学会年会
  • Place of Presentation: 札幌
  • Year and Date: 20080723-20080725
• [Presentation] マイクロRNA前駆体に見出されたDicing部位を規定する基質モチーフとmiRNAの3'末端形成 (2008)
  • Author(s): 加藤敬行、坂口裕理子、宮内健常、鈴木健夫、鈴木勉
  • Organizer: 第10回日本RNA学会年会
  • Place of Presentation: 札幌
  • Year and Date: 20080723-20080725
• [Presentation] 大腸菌23S rRNAの円順列変異の取得とリボソーム構築原理の新知見 (2008)
  • Author(s): 北原圭、鈴木勉
  • Organizer: 第10回日本RNA学会年会
  • Place of Presentation: 札幌
  • Year and Date: 20080723-20080725
• [Presentation] RNA mass spectrometry: a platform technology for non-coding RNA research (2008)
  • Author(s): Yuriko Sakaguchi, Hiroki Ueda, Takeo Suzuki, Takayuki Katoh, Takeshi Seguchi, Kenjyo Miyauchi, Tsutomu Suzuki
  • Organizer: 56th ASMS Conference on Mass Spectrometry
  • Place of Presentation: Denver
  • Year and Date: 20080601-20080605
• [Presentation] Mass spectrometric identification of non-coding RNAs in ribonucleoprotein complexes; biochemical approach to RNA-protein interactome" (2008)
  • Author(s): 鈴木勉
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学大会合同大会(BMB2008)
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-12
• [Presentation] RNA修飾の生合成と機能に関する研究(Biogenesis and functions of RNA modifications) (2008)
  • Author(s): 鈴木勉
  • Organizer: 第6回日本分子生物学会三菱化学奨励賞受賞講演
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-10
• [Presentation] RNAマススペクトロメトリー-機能性RNAの直接解析で見えてきたもの- (2008)
  • Author(s): 鈴木勉
  • Organizer: 2008質量分析計ユーザーズフォーラム
  • Place of Presentation: 東京
  • Year and Date: 2008-10-22
• [Presentation] RNAマススペクトロメトリー-機能性RNAの直接解析で見えてきたもの- (2008)
  • Author(s): 鈴木勉
  • Organizer: 発生工学・疾患モデル研究会「第70回定例会」
  • Place of Presentation: 東京
  • Year and Date: 2008-09-10
• [Presentation] "自分らしい"RNA研究を追い求めてきて (2008)
  • Author(s): 鈴木勉
  • Organizer: RNAフロンティアミーティング2008
  • Place of Presentation: 京都
  • Year and Date: 2008-06-04
• [Presentation] RNAに魅せられて-企業での経験をアカデミックな研究に生かす (2008)
  • Author(s): 鈴木勉
  • Organizer: 東工大生命理工GCOEシンポジウム「ようこそ先輩」
  • Place of Presentation: 長津田
  • Year and Date: 2008-01-28
• [Presentation] Small non-coding RNAの直接解析で見えてきたもの (2007)
  • Author(s): 鈴木勉
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学大会合同大会(BMB2007)
  • Place of Presentation: 横浜
  • Year and Date: 20071211-20071215
• [Presentation] Aminoacyl-tRNA surveillance by EF-Tu in mammalian mitochondria (2007)
  • Author(s): Asuteka Nagao, Takeo Suzuki, Tsutomu Suzuki
  • Organizer: 第5回国際核酸化学シンポジウム(NACS2007)
  • Place of Presentation: 東京
  • Year and Date: 20071120-20071122
• [Presentation] Ligand-induced translation by the allosteric ribosome bearing an aptamer-fused rRNA (2007)
  • Author(s): Takeshi Yokoyama, Tsutomu Suzuki
  • Organizer: 第5回国際核酸化学シンポジウム(NACS2007)
  • Place of Presentation: 東京
  • Year and Date: 20071120-20071122
• [Presentation] Quality control of aminoacyl-tRNAs by kinetic competition of aminoacyltRNA synthetases and EF-Tu surveillance in mammalian mitochondria (2007)
  • Author(s): Asuteka Nagao, Takeo Suzuki, Tsutomu Suzuki
  • Organizer: 22nd International tRNA Workshop
  • Place of Presentation: ウプサラ
  • Year and Date: 20071101-20071106
• [Presentation] Genome-wide identification of genes responsible for 2-thiolation of 5-methoxycarbonylmethyl-2-thiouridine (mcm5s2U) at wobble position of yeast tRNAs (2007)
  • Author(s): Akiko Noma, Tsutomu Suzuki
  • Organizer: 22nd International tRNA Workshop
  • Place of Presentation: ウプサラ
  • Year and Date: 20071101-20071106
• [Presentation] TmcA catalyzes 4-acetylcytidine formation at wobble position of bacterial tRNAMet (2007)
  • Author(s): Yoshiho Ikeuchi, Sarin Chimnaronk, Min Yao, Isao Tanaka, Tsutomu Suzuki
  • Organizer: 22nd International tRNA Workshop
  • Place of Presentation: ウプサラ
  • Year and Date: 20071101-20071106
• [Presentation] Mass spectrometric characterization of small non-coding RNAs (2007)
  • Author(s): Tsutomu Suzuki
  • Organizer: Yonsei University-The University of Tokyo Joint Symposium
  • Place of Presentation: ソウル
  • Year and Date: 20070807-20070709
• [Presentation] 精子形成における哺乳類piRNAメチル化酵素の機能解析 (2007)
  • Author(s): 長岡真、大原智也、中川真一、加藤敬行、五島直樹、野村信夫、鈴木健夫、坂口裕理子、鈴木勉
  • Organizer: 第9回日本RNA学会年会
  • Place of Presentation: 名古屋
  • Year and Date: 20070728-20070731
• [Presentation] Characterization and tissue specificity of A-to-I RNA editing found in 3'UTR of human mRNAs (2007)
  • Author(s): Masayuki Sakurai, Takanori Yano, Tsutomu Suzuki
  • Organizer: Gordon Research Conference, RNA editing
  • Place of Presentation: Ventura
  • Year and Date: 20070114-20070119
• [Presentation] RNA mass spectrometry: a platform technology for non-coding RNA research (2007)
  • Author(s): 鈴木勉
  • Organizer: 日本ヒトプロテオーム機構(JHUPO)第5回大会
  • Place of Presentation: 東京
  • Year and Date: 2007-07-31
• [Presentation] RNAマススペクトロメトリー (2007)
  • Author(s): 鈴木勉
  • Organizer: 日本プロテオーム機構(JHUPO)第5回大会
  • Place of Presentation: 東京
  • Year and Date: 2007-07-31
• [Presentation] RNA修飾が関与する生命現象へのアプローチ (2007)
  • Author(s): 鈴木勉
  • Organizer: RNA分子のダイナミズム-生命現象の根幹をなす機能性RNA
  • Place of Presentation: 名古屋
  • Year and Date: 2007-07-28
• [Presentation] Form and Function Cape Cod, USA MECHANISTIC AND ARCHITECTURAL ANALYSIS OF E (2007)
  • Author(s): Neuza S. Sato, Kei Kitahara, Takeshi Yokoyama, Naomi Hirabayashi, Taeko Komoda, Steven S. Phelps, Simpson Joseph, Rajendra K. Agrawal, Ilana Agmon, Ada Yonath, Tsutomu Suzuki
  • Organizer: Ribosome2007
  • Place of Presentation: COLI RIBOSOMAL RNAS USING THE COMPREHENSIVE GENETIC SELECTION
  • Year and Date: 2007-06-05
• [Presentation] Mass spectrometric characterization of small non-coding RNAs; identification of 2'-O-methylation at the 3'-termini of mouse piwi-interacting RNAs (2007)
  • Author(s): Tsutomu Suzuki, Tomoya Ohara, Takeo Suzuki, Hiroki Ueda, Takeshi Seguchi, Kenjyo Miyauchi, Yuriko Sakaguchi
  • Organizer: RNA 2007: 12th Annual Meeting of the RNA Society
  • Place of Presentation: Madison
  • Year and Date: 2007-06-01
• [Presentation] RNA mass spectrometry reveals qualitative aspects of non-coding RNAs (2007)
  • Author(s): 鈴木勉
  • Organizer: 細胞生物学会/発生生物学会シンポジウム「Frontiers in RNA Biology」
  • Place of Presentation: 福岡
  • Year and Date: 2007-05-30
• [Presentation] RNA修飾の多彩な機能と生命現象 (2007)
  • Author(s): 鈴木勉
  • Organizer: お茶の水がん学アカデミア第34回集会
  • Place of Presentation: 東京
  • Year and Date: 2007-04-24
• [Presentation] 機能性RNAのマススペクトロメトリー～見過ごされているRNAの質的な情報と高次生命現象へのアプローチ～ (2007)
  • Author(s): 鈴木勉
  • Organizer: 化学とバイオの架け橋
  • Place of Presentation: 本郷
  • Year and Date: 2007-04-07
• [Presentation] A1555G変異型アミノグリコシド誘導性難聴におけるミトコンドリア翻訳異常とリボソームタンパク質S12変異体による救済 (2006)
  • Author(s): 鴫成実, 林純一, 上田卓也, 鈴木勉
  • Organizer: 第6回日本ミトコンドリア学会年会
  • Place of Presentation: 横浜
  • Year and Date: 20061214-20061216
• [Presentation] Ribonucleome analysis identified enzyme genes responsible for wybutosine synthesis (2006)
  • Author(s): Akiko Noma, Tsutomu Suzuki
  • Organizer: 東大21COE-ソウル大BK21合同セミナー
  • Place of Presentation: ソウル
  • Year and Date: 20061211-20061212
• [Presentation] Mechanistic and architectural analysis of E. coli ribosomal RNAs using the comprehensive genetic selection (2006)
  • Author(s): Neuza S. Sato, Kei Kitahara, Takeshi Yokoyama, Naomi Hirabayashi, Taeko Komoda, Steven S. Phelps, Simpson Joseph, Rajendra K. Agrawal, Ilana Agmon, Ada Yonath, Tsutomu Suzuki
  • Organizer: RNA 2006 Izu "Functional RNAs and Regulatory Machinery "
  • Place of Presentation: 伊豆
  • Year and Date: 20061203-20061207
• [Presentation] Systematic deletion of rRNAs for investigating ribosome architecture and function (2006)
  • Author(s): Kei Kitahara, Neuza S. Sato, Naoki Namba, Takuya Yokota, Tarou Tujimura, Tsutomu Suzuki
  • Organizer: 第33回核酸化学シンポジウム
  • Place of Presentation: 大阪
  • Year and Date: 20061120-20061122
• [Presentation] Ribonucleome analysis identified enzyme genes responsible for wybutosine synthesis (2006)
  • Author(s): Akiko Noma, Tsutomu Suzuki
  • Organizer: 第33回核酸化学シンポジウム
  • Place of Presentation: 大阪
  • Year and Date: 20061120-20061122
• [Presentation] Insight into the first tRNA cytidine acetyltransferase, TmcA Sarin Chimnaronk, Tetsuhiro (2006)
  • Author(s): Manita, Min Yao, Yoshiho Ikeuchi, Tsutomu Suzuki, Isao Tanaka
  • Organizer: AARS2006
  • Place of Presentation: San Diego
  • Year and Date: 20061001-20061008
• [Presentation] アミノアシルtRNA 合成酵素とEF-Tu の連動による翻訳精度維持機構 (2006)
  • Author(s): 長尾翌手可、サリンチムナロン、鈴木健夫、鈴木勉
  • Organizer: RNA特定領域研究「RNA情報網」 第4回サテライトミーティング「RNA新大陸のフロンティア達」
  • Place of Presentation: 裾野
  • Year and Date: 20060911-20060913
• [Presentation] ヒトmRNA 3'UTRにおけるA to I RNAエディティングの機能解析 (2006)
  • Author(s): 矢野孝紀, 櫻井雅之, 鈴木勉
  • Organizer: 第8回日本RNA学会年会
  • Place of Presentation: 淡路島
  • Year and Date: 20060718-20060720
• [Presentation] RNA修飾に関わる硫黄リレーシステムの発見と反応機構の解析 (2006)
  • Author(s): 池内与志穂, 沼田倫征, 深井周也, 鴫直樹, 加藤潤一, 西村昭子, 濡木理, 鈴木勉
  • Organizer: 第8回日本RNA学会年会
  • Place of Presentation: 淡路島
  • Year and Date: 20060718-20060720
• [Presentation] Mechanistic insights into biogenesis of RNA modifications by multiple protein components (2006)
  • Author(s): Yoshiho Ikeuchi, Akiko Noma, Kenjyo Miyauchi, Takeo Suzuki, Yuriko Sakaguchi, Tsutomu Suzuki
  • Organizer: RNA 2006: 11th Annual Meeting of the RNA Society
  • Place of Presentation: Seattle
  • Year and Date: 20060620-20060625
• [Presentation] Stepwise dicing: human Dicer initially cleaves the strand bearing 3'-overhang and subsequently cuts another strand (2006)
  • Author(s): Shinya Kurata, Takayuki Katoh, Naoki Goshima, Nobuo Nomura, Tsutomu Suzuki
  • Organizer: RNA 2006: 11th Annual Meeting of the RNA Society
  • Place of Presentation: Seattle
  • Year and Date: 20060620-20060625
• [Presentation] Mechanistic Insights into Sulfur-Relay by Novel Sulfur Mediators Involved in Thiouridine Biosynthesis at tRNA Wobble Positions. (2006)
  • Author(s): Yoshiho Ikeuchi, Naoki Shigi, Jun-ichi Kato, Akiko Nishimura, Tsutomu Suzuki
  • Organizer: the 20th IUBMB International Congress of Biochemistry and Molecular Biology
  • Place of Presentation: 京都
  • Year and Date: 20060618-20060623
• [Presentation] Structural analysis of protein complexes using the isotope-tagging mass spectrometric footprinting (2006)
  • Author(s): Yuriko Sakaguchi, Yusuke Nozaki, Tsutomu Suzuki
  • Organizer: the 20th IUBMB International Congress of Biochemistry and Molecular Biology
  • Place of Presentation: 京都
  • Year and Date: 20060618-20060623
• [Presentation] リボヌクレオーム解析を用いた酵母RNA修飾遺伝子の網羅的探索 (2006)
  • Author(s): 野間章子、鈴木勉
  • Organizer: 第6回日本蛋白質科学会年会
  • Place of Presentation: 京都
  • Year and Date: 20060424-20060426
• [Presentation] RNA修飾の多彩な機能と生命現象 (2006)
  • Author(s): 鈴木勉
  • Organizer: 第17回フォーラム・イン・ドージン「生命活動を支えるRNAプログラム」
  • Place of Presentation: 熊本
  • Year and Date: 2006-11-17
• [Presentation] RNA修飾の世界 (2006)
  • Author(s): 鈴木勉
  • Organizer: 電気泳動学会シンポジウム
  • Place of Presentation: 浜松
  • Year and Date: 2006-10-27
• [Presentation] Ligand-induced translation by the ribosome bearing an aptamer-introduced rRNA (2006)
  • Author(s): Takeshi yokoyama, Tsutomu Suzuki
  • Organizer: International workshop on synthetic bology "Synthetic Approaches to Cellular Functions"
  • Place of Presentation: 東京
  • Year and Date: 2006-10-12
• [Presentation] 大腸菌tRNAウォブル位修飾ウリジンの側鎖構造炭素源の決定 (2006)
  • Author(s): 鈴木健夫, 鈴木勉
  • Organizer: 第3回21世紀大腸菌研究会
  • Place of Presentation: 滋賀
  • Year and Date: 2006-10-03
• [Presentation] 精密質量分析計を用いた高感度RNA解析法の構築 (2006)
  • Author(s): 鈴木健夫、坂口裕里子、上田宏生、宮内健常、鈴木勉
  • Organizer: 第8回日本RNA学会年会
  • Place of Presentation: 淡路島
  • Year and Date: 2006-08-01
• [Presentation] リボヌクレオーム解析を用いたRNA修飾遺伝子の網羅的解析 (2006)
  • Author(s): 鈴木勉
  • Organizer: 京都大学COEケミカルバイオロジーミニシンポジウム一味違う化学と生命現象の接点を目指して
  • Place of Presentation: 京都
  • Year and Date: 2006-03-16
• [Presentation] RNA修飾に関わる硫黄リレータンパク質群の同定 (2006)
  • Author(s): 池内与志穂、鴫直樹、加藤潤一、西村昭子、鈴木勉
  • Organizer: 第8回微生物ゲノムのフロンティアワークショップ
  • Place of Presentation: かずさ
  • Year and Date: 2006-03-03
• [Presentation] Molecular pathogenesis of human mitochondrial disease caused by tRNA wobble modification deficiency (2005)
  • Author(s): Kirino, Y., Goto, Y., Campos, Y., Arenas, J., Taylor, R.W., Suzuki., T.
  • Organizer: RNA2005:10th Annual Meeting of the RNA Society
  • Place of Presentation: Banff
  • Year and Date: 20050524-20050529
• [Presentation] Identification and characterization of four new genes responsible for biosynthesis of wybutosine, a hyper-modified nucleoside in eukaryotic phenylalanine tRNA (2005)
  • Author(s): Akiko Noma, Yohei Kirino, Yoshiho Ikeuchi, Tsutomu Suzuki
  • Organizer: 21st International tRNA Workshop
  • Place of Presentation: バンガロール
  • Year and Date: 2005-12-01
• [Presentation] Mechanistic insights into sulfur-relay by novel sulfur mediators involved in thiouridine biosynthesis at tRNA wobble positions (2005)
  • Author(s): Yoshiho Ikeuchi, Naoki Shigi, Jun-ichi Kato, Akiko Nishimura, Tsutomu Suzuki
  • Organizer: 21st International tRNA Workshop
  • Place of Presentation: バンガロール
  • Year and Date: 2005-12-01
• [Presentation] Molecular pathogenesis of human mitochondrial diseases caused by tRNA wobble modification deficiency (2005)
  • Author(s): Yohei Kirino, Yu-ichi Goto, Yolanda Campos, Joaquin Arenas, Robert W. Taylor, Tsutomu Suzuki
  • Organizer: 21st International tRNA Workshop
  • Place of Presentation: バンガロール
  • Year and Date: 2005-12-01
• [Presentation] Automatic parallel purification of tRNAs and non-coding RNAs by the reciprocal circulating chromatography method (2005)
  • Author(s): Kenjyo Miyauchi, Tomoya Ohara, Tsutomu Suzuki
  • Organizer: 21st International tRNA Workshop
  • Place of Presentation: バンガロール
  • Year and Date: 2005-12-01
• [Presentation] Molecular mechanism of lysidine synthesis that determines tRNA identity and codon recognition (2005)
  • Author(s): Yoshiho Ikeuchi, Akiko Soma, Yasuhiko Sekine, Tsutomu Suzuki
  • Organizer: 21st International tRNA Workshop
  • Place of Presentation: バンガロール
  • Year and Date: 2005-12-01
• [Presentation] 往復循環クロマトグラフィーによるmulti-ChIP法の開発 (2005)
  • Author(s): 大平高之宮内健常鈴木勉
  • Organizer: 日本分子生物学会
  • Place of Presentation: 福岡
  • Year and Date: 2005-12-01
• [Presentation] Molecular pathogenesis of human diseases associated with tRNA wobble modification disorder (2005)
  • Author(s): Tsutomu Suzuki
  • Organizer: FinMIT-JMIT meeting
  • Place of Presentation: Helsinki
  • Year and Date: 2005-11-28
• [Presentation] Molecular pathogenesis of human mitochondrial diseases caused by tRNA wobble modification deficiency Yohei Kirino, Yu-ichi Goto, Yolanda Campos, Joaquin Arenas (2005)
  • Author(s): Robert W. Taylor, Tsutomu Suzuki
  • Organizer: 第78回日本生化学会「機能性RNAの新展開」
  • Place of Presentation: 神戸
  • Year and Date: 2005-10-22
• [Presentation] ライシジン合成酵素を標的とした抗生物質の開発をめざして (2005)
  • Author(s): 鈴木勉
  • Organizer: 感染の成立と宿主応答の分子基盤(若手研究部会)
  • Place of Presentation: 東京
  • Year and Date: 2005-10-07
• [Presentation] 往復循環クロマトグラフィー法による機能性RNAの全自動単離精製 (2005)
  • Author(s): 宮内健常, 大原智也, 鈴木勉
  • Organizer: 第7回日本RNA学会年会
  • Place of Presentation: 弘前
  • Year and Date: 2005-08-10
• [Presentation] The A site finger (H38) in the 23S rRNA works as a negative regulator of translocation (2005)
  • Author(s): Taeko Komoda, Neuza Satomi Sato, Steven S. Phelps, Simpson Joceph, Tsutomu Suzuki
  • Organizer: 第7回日本RNA学会年会
  • Place of Presentation: 弘前
  • Year and Date: 2005-08-10
• [Presentation] ヒト・マウスmRNA/non-coding RNAにおける新規RNAエディティング部位の網羅的探索 (2005)
  • Author(s): 櫻井雅之, 矢野孝紀, 鈴木勉
  • Organizer: 第7回日本RNA学会年会
  • Place of Presentation: 弘前
  • Year and Date: 2005-08-10
• [Presentation] The conserved sequence of helix 69 in the E. coli 23S rRNA is involved in the subunit association, A site tRNA binding and translational fidelity Naomi Hirabayashi, Neuza Satomi (2005)
  • Author(s): Sato, Tsutomu Suzuki
  • Organizer: 第7回日本RNA学会年会
  • Place of Presentation: 弘前
  • Year and Date: 2005-08-10
• [Presentation] 系統的挿入変異を用いたリボソームRNAの分子進化と機能解析 (2005)
  • Author(s): 横山武司, 北原圭, 鈴木勉
  • Organizer: 第7回日本RNA学会年会
  • Place of Presentation: 弘前
  • Year and Date: 2005-08-10
• [Presentation] リボヌクレオーム解析を用いたRNA修飾遺伝子の網羅的解析 (2005)
  • Author(s): 鈴木勉
  • Organizer: 第5回日本蛋白科学会
  • Place of Presentation: 福岡
  • Year and Date: 2005-07-22
• [Presentation] 安定同位体標識フットプリント法を用いたタンパク質の高次構造変化の解析 (2005)
  • Author(s): 鈴木勉
  • Organizer: 蛋白研セミナー「蛋白質の質量分析-安定同位体標識による新たな展開」
  • Place of Presentation: 大阪
  • Year and Date: 2005-07-20
• [Presentation] リボヌクレオーム解析を用いた大腸菌RNA修飾遺伝子の網羅的探索 (2005)
  • Author(s): 鈴木勉
  • Organizer: 国立遺伝研シンポジウム「モデル生物・大腸菌の歩み:10年後を見据えて」
  • Place of Presentation: 三島
  • Year and Date: 2005-03-28
• [Presentation] 機能性rnaにおける転写後修飾の役割と疾患との関係 (2005)
  • Author(s): 鈴木勉
  • Organizer: 千里ライフサイエンスシンポジウム「RNA機能研究の最先端」
  • Place of Presentation: 大阪
  • Year and Date: 2005-02-25
• [Presentation] rna干渉を用いた遺伝子発現抑制技術と創薬への展望 (2005)
  • Author(s): 鈴木勉
  • Organizer: 日本テクノセンターセミナー「RNA干渉を用いた遺伝子発現抑制技術と創薬への展望」
  • Place of Presentation: 東京
  • Year and Date: 2005-02-16
• [Presentation] Biosynthesis of tRNA wobble modification and molecular pathogenesis of human diseases associated with RNA modification disorder (2005)
  • Author(s): Tsutomu Suzuki
  • Organizer: Gordon Research Conference[RNA editing and modification]
  • Place of Presentation: ベンチュラ
  • Year and Date: 2005-01-27
• [Presentation] rna干渉を用いた効果的な遺伝子発現抑制技術 (2005)
  • Author(s): 鈴木勉
  • Organizer: 科学技術振興機構JST主催「ゲノムリテラシー講座」
  • Place of Presentation: 東京
  • Year and Date: 2005-01-11
• [Presentation] ミトコンドリアtRNALeuのタウリン修飾は欠損はMELAS患者に特異的である-プライマー伸長法を応用した超微量RNA修飾解析法の確立- (2004)
  • Author(s): 桐野陽平、後藤雄一、Yolanda Campos、Joaquin Arenas、鈴木勉
  • Organizer: 第6回日本RNA学会年会
  • Place of Presentation: 熊本
  • Year and Date: 20040804-20040806
• [Presentation] フィンランドサマーセミナー (2004)
  • Author(s): Tsutomu Suzuki
  • Organizer: 「Biosynthesis and function of RNA modifications」「Molecular pathogenesis of human diseases associated with RNA modification disorder」「Mechanistic and architectural evolution of ribosome」
  • Place of Presentation: Murikka, Tampere
  • Year and Date: 20040724-20040804
• [Presentation] 1555位変異型難聴におけるミトコンドリア翻訳障害 (2004)
  • Author(s): 日野成実、林純一、上田卓也、鈴木勉
  • Organizer: 第4回日本ミトコンドリア研究会年会
  • Place of Presentation: 東京
  • Year and Date: 2004-12-17
• [Presentation] ミトコンドリアtRNALeuのタウリン修飾欠損はMELAS患者に特異的である-プライマー伸長法を応用した超微量RNA修飾解析法の確立- (2004)
  • Author(s): 桐野陽平、後藤雄一、Yolanda Campos、Joaquin Arenas、鈴木勉
  • Organizer: 第4回日本ミトコンドリア研究会年会
  • Place of Presentation: 東京
  • Year and Date: 2004-12-17
• [Presentation] リボヌクレオーム解析を用いたRNA修飾遺伝子の網羅的探索 (2004)
  • Author(s): 池内与志穂、野間章子、相馬亜希子、加藤潤一、西村昭子、三木健良、小林和夫、小笠原直毅、関根靖彦、鈴木勉
  • Organizer: 第27回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2004-12-11
• [Presentation] 大腸菌tRNAウォブル位に存在する修飾ウリジンの側鎖構造炭素源の決定と生合成機構の解析 (2004)
  • Author(s): 鈴木健夫、鈴木勉
  • Organizer: 第27回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2004-12-11
• [Presentation] RNAにおける転写後修飾の役割と疾患 (2004)
  • Author(s): 鈴木勉
  • Organizer: 東京大学の生命科学シンポジウム
  • Place of Presentation: 本郷
  • Year and Date: 2004-12-04
• [Presentation] RNA干渉における三塩基周期性と活性予測 (2004)
  • Author(s): 鈴木勉
  • Organizer: 第14回アンチセンスシンポジウム
  • Place of Presentation: 長津田
  • Year and Date: 2004-12-03
• [Presentation] を用いた効果的な遺伝子発現抑制技術の開発ー夢のRNAi医薬を目指して (2004)
  • Author(s): 鈴木勉
  • Organizer: 第17回日本放射線腫瘍学会
  • Place of Presentation: 幕張
  • Year and Date: 2004-11-18
• [Presentation] Ribonucleome, the World of RNA Modifications (2004)
  • Author(s): Tsutomu Suzuki
  • Organizer: 2nd COE International symposium on human-friendly materials based on chemistry: better living through innovative biomaterials(Takeda hall)
  • Place of Presentation: 本郷
  • Year and Date: 2004-11-09
• [Presentation] RNAi活性予測アルゴリズムと新規転写合成dsRNAを用いた効果的な遺伝子発現抑制技術 (2004)
  • Author(s): 鈴木勉
  • Organizer: 第77回日本生化学会バイオインダストリーセミナー
  • Place of Presentation: 横浜
  • Year and Date: 2004-10-14
• [Presentation] RNA-modifying enzyme that governs both the codon and amino acid specificities of isoleucine tRNA. Yoshiho Ikeuchi, Akiko Soma, Satoru Kanemasa, Kazuo Kobayashi, Naotake Ogasawara, Tomotake Ote, Jun-ichi Kato, Kimitsuna Watanabe (2004)
  • Author(s): Yasuhiko Sekine, Tsutomu Suzuki
  • Organizer: The awaji international forum on infection and immunity IV, A novel target of antibiotics
  • Place of Presentation: 淡路島
  • Year and Date: 2004-08-31
• [Presentation] Biosynthesis and function of tRNA wobble modifications (2004)
  • Author(s): Tsutomu Suzuki
  • Organizer: 第5回コロッキウム
  • Place of Presentation: ウプサラ
  • Year and Date: 2004-06-15
• [Presentation] 転写合成dsRNAを利用した遺伝子発現抑制法 (2004)
  • Author(s): 加藤敬行、鈴木勉
  • Organizer: 日本生化学会近畿支部会
  • Place of Presentation: 京都
  • Year and Date: 2004-06-05
• [Presentation] RNA修飾遺伝子の網羅的探索 (2004)
  • Author(s): 池内与志穂、相馬亜希子、加藤潤一、西村昭子、三木健良、関根靖彦、渡辺公綱、鈴木勉
  • Organizer: 21世紀大腸菌研究会
  • Place of Presentation: 沼津
  • Year and Date: 2004-06-01
• [Presentation] リボヌクレオームー機能性RNAが活躍する転写後の世界 (2004)
  • Author(s): 鈴木勉
  • Organizer: 第3回国際バイオEXPO
  • Place of Presentation: 東京
  • Year and Date: 2004-05-20
• [Book] ミトコンドリア脳筋症とtRNAのタウリン修飾欠損(細胞工学2月号) (2010)
  • Author(s): 鈴木勉、鈴木健夫
  • Total Pages: 137-143
• [Book] マススペクトロメトリーを用いたmiRNAの直接プロファイリング(分子細胞治療, vol.8(No.5)) (2009)
  • Author(s): 坂口裕理子、鈴木勉
  • Total Pages: 36-41
• [Book] 細胞質に局在するGLD-2による3'末端アデニル化とmiRNAの選択的安定化機構(実験医学, Vol.27(No.11)7月号) (2009)
  • Author(s): 加藤敬行、鈴木勉
  • Total Pages: 1746-1750
• [Book] mRNAの塩基修飾・編集と遺伝子発現制御機構(蛋白質 核酸 酵素, 12月増刊号) (2009)
  • Author(s): 櫻井雅之、矢野孝紀、岡田俊平、竹内祐樹、鈴木勉
  • Total Pages: 2086-2091
  • Publisher: 共立出版
• [Book] 核酸アナログとsiRNAの配列設計(核酸医薬の最前線) (2009)
  • Author(s): 加藤敬行、宮内健常、鈴木勉
  • Publisher: シーエムシー出版
• [Book] 転写後修飾によるRNA機能制御(細胞工学(2月号), vol.28(No.2)) (2009)
  • Author(s): 櫻井雅之、鈴木勉
  • Total Pages: 149-155
  • Publisher: 秀潤社
• [Book] 大腸菌からのRNA抽出(RNA実験ノート上(RNAの基本的な取り扱いから解析手法まで)) (2008)
  • Author(s): 宮内健常、鈴木勉
  • Total Pages: 17-20
  • Publisher: 羊土社
• [Book] 質量分析法を用いたRNA修飾ヌクレオシドの解析(RNA実験ノート上(RNAの基本的な取り扱いから解析手法まで)) (2008)
  • Author(s): 鈴木健夫、鈴木勉
  • Total Pages: 177-181
  • Publisher: 羊土社
• [Book] 有効なsiRNAのデザインと細胞への導入(RNA実験ノート下(小分子RNAの解析からRNAiへの応用まで)) (2008)
  • Author(s): 加藤敬行、鈴木勉
  • Total Pages: 99-102
  • Publisher: 羊土社
• [Book] RNAとゲノム(最近の話題)-座談会機能性RNA研究の展望-(ゲノム医学, Vol.7(No.2)) (2007)
  • Author(s): 渡辺公綱、浅井潔、鈴木勉、廣瀬哲郎
  • Total Pages: 79-84
• [Book] マススペクトロメトリーによるncRNAの解析(RNAと生命(蛋白質核酸酵素12月号増刊)) (2006)
  • Author(s): 鈴木勉、鈴木健夫、上田宏生、宮内健常、坂口裕理子
  • Publisher: 共立出版
• [Book] RNA修飾酵素の細胞内局在とRNA成熟化機構(細胞核の世界(蛋白質核酸酵素11月号増刊)) (2006)
  • Author(s): 野間章子、鈴木勉
  • Publisher: 共立出版
• [Book] RNAマススペクトロメトリー(ゲノム医学, Vol.6) (2006)
  • Author(s): 鈴木勉、鈴木健男、坂口裕理子
  • Total Pages: 89-96
  • Publisher: メディカルレヴュー社
• [Book] 遺伝暗号の変遷と進化的普遍性(蛋白質 核酸 酵素) (2006)
  • Author(s): 渡辺公綱、鈴木勉
  • Publisher: 共立出版
• [Book] RNA修飾に関わる硫黄リレータンパク質群の同定(細胞工学(4月号)) (2006)
  • Author(s): 池内与志穂, 鈴木勉
  • Publisher: 秀潤社
• [Book] RNA修飾の世界-見過ごされているRNAの質的な情報(生化学会誌12月号) (2005)
  • Author(s): 鈴木勉
• [Book] リボソームの立体構造と抗生物質の作用機所(監修中村義一, RNA工学の最前線) (2005)
  • Author(s): 北原圭、鈴木勉
  • Publisher: シーエムシー出版
• [Book] 哺乳動物ミトコンドリアの翻訳系-ミトコンドリアゲノムの縮小によってもたらされたユニークなシステム(蛋白質 核酸 酵素(3月増刊号), 二層膜オルガネラの遺伝学) (2005)
  • Author(s): 鴫成実、鈴木勉
  • Publisher: 共立出版
• [Book] MELASにおけるA3243G変異とtRNA修飾異常(臨床検査, Vol.49(No.1)) (2005)
  • Author(s): 桐野陽平、鈴木勉
  • Total Pages: 89-95
  • Publisher: 医学書院
• [Book] RNAiにおけるsiRNAの合理的配列設計法(RNAi法とアンチセンス法-RNAの科学と応用) (2005)
  • Author(s): 鈴木勉、加藤敬行
  • Publisher: 講談社サイエンティフィク
• [Book] RNA干渉による新しい遺伝子発現制御技術と創薬(テクノニュースちば) (2005)
  • Author(s): 鈴木勉
• [Book] 機能性RNAのマススペクトロメトリー(躍進するRNA研究, 実験医学11月増刊号) (2004)
  • Author(s): 鈴木勉、池内与志穂、鈴木健夫
• [Book] 実験医学特集「解明が進むRNAの成熟化プロセス」(監修 鈴木勉、廣瀬哲郎, 機能性RNA研究におけるRNAプロセシングの重要性, 実験医学(7月号))
  • Author(s): 鈴木勉、廣瀬哲郎
  • Publisher: 羊土社
• [Book] 実験医学特集「解明されるRNAの成熟化プロセス」(監修 鈴木 勉、廣瀬哲郎, RNA修飾の生合成と機能, 実験医学(7月号))
  • Author(s): 鈴木勉
  • Publisher: 羊土社
• [Remarks] ホームページ等
  • URL: http://rna.chem.t.u-tokyo.ac.jp/cgi/siexplorer.htm
• [Remarks] siExplorer:effective siRNA design algorithm
• [Patent(Industrial Property Rights)] イソロイシンtRNA(tRNAIle)のライシジン合成酵素(TilS)としてのmesJ遺伝子産物及びその相同性遺伝子(COG0037) (2010)
  • Inventor(s): 鈴木勉
  • Industrial Property Rights Holder: 東京大学
  • Industrial Property Number: 特許特願2003-329762
  • Filing Date: 2010-04-09
• [Patent(Industrial Property Rights)] RNA中のイノシン化部位の検出方法 (2005)
  • Inventor(s): 鈴木勉、櫻井雅之
  • Industrial Property Rights Holder: 東京大学
  • Industrial Property Number: 特許特願2005-229335 PCT/JP2006/315581
  • Filing Date: 2005-08-08
• [Patent(Industrial Property Rights)] 往復循環クロマトグラフィーを用いた生体高分子の単離方法 (2005)
  • Inventor(s): 鈴木勉、宮内健常
  • Industrial Property Rights Holder: 東京大学
  • Industrial Property Number: 特許特願2005-224992, PCT/JP2006/315271
  • Filing Date: 2005-08-03