2009 Fiscal Year Final Research Report
Comparative genomics of chromosome rearrangement in vertebrates based on medaka genome information
| Project/Area Number |
17018016
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| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | Nagoya University |
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Principal Investigator |
HORI Hiroshi Nagoya University, 大学院・理学研究科, 名誉教授 (60116663)
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| Co-Investigator(Kenkyū-buntansha) |
MITANI Hiroshi 東京大学, 大学院・新領域創成科学研究科, 教授 (70181922)
SASAKI Takashi 慶応大学, 医学部, 助手 (70306843)
SHIMIZU Atsushi 慶応大学, 医学部, 助手 (30327655)
KANAMORI Akira 名古屋大学, 大学院・理学研究科, 助教 (40324389)
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| Project Period (FY) |
2005 – 2009
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| Keywords | メダカ / ゲノム / BACライブラリー / 染色体再編 / 比較ゲノム |
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| Research Abstract |
Evolution of the genome size in vertebrates is often affected by changes in the noncoding sequences, for which insertions and deletions (indels) of small nucleotide sequences and amplification of repetitive elements are considered responsible. In this project, we especially compared the genomic DNA sequences of two kinds of fish, medaka (Oryzias latipes) and fugu (Takifugu rubripes), which show two-fold difference in the genome size (800 Mb vs. 400 Mb). We determined a contiguous DNA sequence of 790 kb from the medaka chromosome LG22 (linkage group 22). The sequence was aligned common between two fishes, and were found abundant in various repetitive elements including many types of unclassified low copy repeats, all of which accounted for more than a half (54%) of the genome size difference. These results strongly indicated that amplification of repetitive elements and compilation of indels are major driving forces to facilitate changes in the genome size.
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[Journal Article] Comparative genomics approach to the expression of fig_alpha, one of the earliest marker genes of oocyte differentiation in medaka (Oryzias latipes)2008
Author(s)
Kanamori, A., Toyama, K., Kitagawa, S., Kamehara, A., Higuchi, T., Kinoshita, M.,, Hori, H.
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Journal Title
Peer Reviewed
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[Journal Article] The DNA sequence of medaka chromosome LG222007
Author(s)
Sasaki T, Shimizu A, Ishikawa SK, Imai S, Asakawa S, Murayama Y, Khorasani MZ, Mitani H, Furutani-Seiki M, Kondoh H, Nanda I, Schmid M, Schartl M, Nonaka M, Takeda H, Hori H, Himmelbauer H, Shima A, Shimizu N.
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Journal Title
Genomics 89(1)
Pages: 124-133
Peer Reviewed
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[Journal Article] Genomic organization of the sex-determining and adjacent regions of the sex chromosomes of medaka2006
Author(s)
Kondo M, Hornung U, Nanda I, Imai S, Sasaki T, Shimizu A, Asakawa S, Hori H, Schmid M, Shimizu N, Schartl M
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Journal Title
Genome Res. 16(7)
Pages: 815-26
Peer Reviewed
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[Journal Article] Organization and structure of hox gene loci in medaka genome and comparison with those of pufferfish and zebrafish genomes2006
Author(s)
Kurosawa G, Takamatsu N, Takahashi M, SumitomoM, SanakaE, Yamada K, Nishi K, Matsuda M, Asakawa S, Ishiguro K, KurosawaY, Shimizu N, Kohara Y, Hori H
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Journal Title
Peer Reviewed
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