2009 Fiscal Year Final Research Report
Functional genomics using transgenic zebrafish
| Project/Area Number |
17018034
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| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | National Institute of Genetics |
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Principal Investigator |
KAWAKAMI Koichi National Institute of Genetics, 個体遺伝研究系, 教授 (70195048)
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| Project Period (FY) |
2005 – 2009
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| Keywords | 動物ゲノム / ゲノム機能発現 / 遺伝子発現調節 / ゲノムデータベース |
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| Research Abstract |
We successfully developed novel genetic methods by using the Tol2 transposon system such as gene trapping, enhancer trapping, the Gal4-UAS system and in vivo transposition system. By utilizing these methods, we created more than 600 of transgenic fish lines that express GFP or Gal4 in specific tissues, cells and organs. By analyzing these transgenic fish, we studied the function of important developmental genes. Further, we constructed a database of the transgenic fish resource and facilitated collaboration with researchers all over the world. Thus, our achievements greatly contributed advances of the study of vertebrate genes.
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