2009 Fiscal Year Final Research Report
Molecular mechanisms for production of useful proteins in Aspergillus fungi based on genomic information
| Project/Area Number |
17019001
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
|
| Allocation Type | Single-year Grants |
|---|
| Review Section |
Biological Sciences
|
|---|
| Research Institution | Tohoku University |
|---|
Principal Investigator |
GOMI Katsuya Tohoku University, 大学院・農学研究科, 教授 (60302197)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
ABE Keietsu 東北大学, 大学院・農学研究科, 教授 (50312624)
KOBAYASHI Tetsuo 名古屋大学, 大学院・生命農学研究科, 教授 (20170334)
KITAMOTO Katsuhiko 東北大学, 大学院・農学生命科学研究科, 教授 (20272437)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
SHINTANI Takahiro 東北大学, 大学院・農学研究科, 准教授 (70374973)
|
|---|
| Project Period (FY) |
2005 – 2009
|
| Keywords | 糸状菌 / コウジカビ / 転写制御因子 / シグナル伝達 / タンパク質分泌 / マイクロアレイ / 相同組換え |
|---|
| Research Abstract |
Based on genome database of koji-mold (Aspergillus oryzae), approximately 400 genes encoding transcription factor have been found, and a library for strains that express those factor genes at high level has been constructed. By using this library, several transcription factors involved in production of industrially important enzymes such as protease, cellulase, lipase, and so on, have been found. Comprehensive analyses of genes/proteins in signal transduction pathway have revealed the novel histidine kinase involved in anaerobic response and the first direct biochemical evidence for the existence of His-Asp phosphotransfer systems in filamentous fungi. In addition, yield of heterologous protein production has been improved by using protease-low-producer strains.
|
