Analysis in pathogenesis of bacterial lethal soft-tissue infection and application to prevention and therapy.

2009 Fiscal Year Final Research Report

• Project/Area Number: 17019021
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Kanazawa University
• Principal Investigator: SHIMIZU Tohru Kanazawa University, 医学系, 教授 (80235655)
• Co-Investigator(Kenkyū-buntansha): OHTANI Kaori 金沢大学, 医学系, 講師 (30377410) ; NAKAGAWA Ichiro 東京医科歯科大学, 医歯薬総合研究科, 教授 (70294113)
• Project Period (FY): 2005 – 2009
• Keywords: 細菌感染症 / ゲノム解析 / トランスクリプトーム / 遺伝子発現制御 / 病原性

Research Abstract

In Clostridium perfringens and Streptococcus pyogenes, both of which causes severe soft-tissue infection (flesh-eating bacteria), we proceed comprehensive analyses by genome sequencing and DNA microarray experiments. We found that these bacteria share common mechanism using small non-coding RNA for the control of virulence expression and that both have different unique mechanism for genome maintenance and total regulatory system for virulence control. These research would be important for finding molecular targets for prevention and/or therapy, which will lead to the innovation of new bacterial infection control.

Research Products

• [Journal Article] Identification of a two-component VirR/VirS regulon in Clostridium perfringens (2010)
  • Author(s): Ohtani, K., Hirakawa, H., Tashiro, K., Yoshizawa, S., Kuhara, S., Shimizu, T.
  • Journal Title: Anaerobe (in press)
  • Peer Reviewed
• [Journal Article] Genetic and biochemical analysis of a class C nonspecific acid phosphatase (NSAP) of Clostridium perfringens (2010)
  • Author(s): Wang, R., Ohtani, K., Wang, Y., Yuan, Y., Hassan, S., Shimizu, T.
  • Journal Title: Microbiol 156 Pages: 167-173
  • Peer Reviewed
• [Journal Article] Contact with enterocyte-like Caco-2 cells induces rapid upregulation of toxin production by Clostridium perfringens type C isolates (2009)
  • Author(s): Vidal, J.E., Ohtani, K., Shimizu, T., McClane, B.A.
  • Journal Title: Cell. Microbiol. 11 Pages: 1306-1328
  • Peer Reviewed
• [Journal Article] Virulence gene regulation by the agr system in Clostridium perfringens (2009)
  • Author(s): Ohtani, K., Yuan, Y., Hassan, S., Wang, R., Wang, Y., Shimizu, T.
  • Journal Title: J. Bacteriol. 191 Pages: 3919-3927
  • Peer Reviewed
• [Journal Article] Comparative genomic analyses of Streptococcus mutans provide insights into chromosomal shuffling and species-specific content (2009)
  • Author(s): Maruyama, F., Kobata, M., Kurokawa, K., Nishida, K., Sakurai, A., Nakano, K., Nomura, R., Kawabata, S., Ooshima, T., Nakai, K., Hattori, M., Hamada, S., Nakagawa, I.
  • Journal Title: BMC Genomics 10 Pages: 358
  • Peer Reviewed
• [Journal Article] Cost-effective DNA sequencing and template preparation from bacterial colony and plasmid (2009)
  • Author(s): Maruyama, F., Nozawa, T., Aikawa, C., Sakurai, A., Nakagawa, I.
  • Journal Title: J. Biosci. Bioeng. 107 Pages: 471-473
  • Peer Reviewed
• [Journal Article] Streptococcus pyogenes degrades extracellular matrix in chondrocytes via MMP-13, Biochem. (2008)
  • Author(s): Sakurai, A., Okahashi, N., Maruyama, F., Ooshima, T., Hamada, S., Nakagawa, I.
  • Journal Title: Biophys. Res. Commun. 373 Pages: 450-454
  • Peer Reviewed
• [Journal Article] Demonstration of mother to child transmission of Streptococcus mutans using multilocus sequence typing (2008)
  • Author(s): Lapirattanakul, J., Nakano, K., Nomura R., Hamada, S., Nakagawa, I., Ooshima, T
  • Journal Title: Caries Res. 42 Pages: 466-474
  • Peer Reviewed
• [Journal Article] Characterization of Genes Regulated Directly by the VirR/VirS System in Clostridium perfringens (2008)
  • Author(s): Okumura, K., Ohtani, K., Hayashi, H., Shimizu, T.
  • Journal Title: J. Bacteriol. 190(23) Pages: 7719-7727
  • Peer Reviewed
• [Journal Article] Carbon catabolite repression of type IV pilus-dependent gliding motility in the anaerobic pathogen Clostridium perfringens (2008)
  • Author(s): Mendez, M., Huang, I.H., Ohtani, K., Grau, R., Shimizu, T., Sarker, M.R.
  • Journal Title: J. Bacteriol. 190 Pages: 48-60
  • Peer Reviewed
• [Journal Article] Streptococcus mutans Exhibits Clonal Variation as Revealed by Multilocus Sequence Typing (2007)
  • Author(s): Nakano, K., Nakano, K,, Lapirattanakul, J., Nomura, R., Nemoto, H., Alaluusua, S., Gronroos, L., Vaara, M., Hamada, S., Ooshima, T., Nakagawa, I.
  • Journal Title: J. Clin. Microbiol. 45 Pages: 2616-2625
  • Peer Reviewed
• [Journal Article] Involvement of Hrs in Destruction of Group A Streptococcus (GAS):Regulation of Autophagosome Maturation (2007)
  • Author(s): Tamai, K., Tanaka, K., Nara, A., Yamamoto, A., Nakagawa, I., Yoshimori, T., Ueno, Y., Shimosegawa, T., Sugamura K.
  • Journal Title: Biochem. Biophys. Res. Commun. 360 Pages: 721-727
  • Peer Reviewed
• [Journal Article] Virulence of Porphyromonas gingivalis is altered by substitution of fimbria gene with different genotype (2007)
  • Author(s): Kato T, Kawai S, Nakano K, Inaba H, Kuboniwa M, Nakagawa I, Tsuda K, Omori H, Ooshima T, Yoshimori T, Amano A.
  • Journal Title: Cell. Microbiol. 9 Pages: 753-765
  • Peer Reviewed
• [Journal Article] Streptocccus mutans exhibits clonal variations as revealed by multilocus sequence typing (2007)
  • Author(s): Nakano N, Lapirattanakul J, Nomura R, Nemoto H, Alaluusua S, Groroos L., Vaara M, Hamada S, Ooshima T, Nakagawa I.
  • Journal Title: J. Clin. Microbiol., J. Clin. Microbiol. 45 Pages: 2616-2625
  • Peer Reviewed
• [Journal Article] Autophagy in innate immunity against intracellular bacteria. (2006)
  • Author(s): Amano A, Nakagawa I, Yoshimori T.
  • Journal Title: J. Biochem. (Tokyo) 140 Pages: 161-166
  • Peer Reviewed
• [Journal Article] Role of glucose side chains with serotype-specific polysaccharide in the cariogenicity of Streptococcus mutans (2005)
  • Author(s): Nakano, K., Nomura, R., Nakagawa, I., Hamada, S., Ooshima, T.
  • Journal Title: Caries Res. 39 Pages: 262-268
  • Peer Reviewed
• [Journal Article] Identification and characterization of a cell-wall anchored DNase gene in Clostridium perfringens (2005)
  • Author(s): Okumura, K., Kawsar, H.I., Shimizu, T., Ohta T.,, Hayashi H., Shimizu, T.
  • Journal Title: FEMS Microbiol. Lett. 242 Pages: 281-285
  • Peer Reviewed
• [Presentation] Complex regulatory networks for virulence in Clostridium perfringens (2009)
  • Author(s): Shimizu, T
  • Organizer: The 6th Clostridia: The Impact of Genomics on Disease Control
  • Place of Presentation: Instito Superiore di Sanita
  • Year and Date: 2009-10-21
• [Presentation] Biological signaling to gene expression in Clostridium perfringens (2006)
  • Author(s): Ohtani, K
  • Organizer: The 5th Clostpath 2006
  • Place of Presentation: Hugh Stewart Hall, Nottingham Univ., U.K.
  • Year and Date: 2006-06-24