Integrative studies on cortico -basal ganglia link involved in organizing actions

2009 Fiscal Year Final Research Report

• Project/Area Number: 17021050
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Tokyo Metropolitan Organization for Medical Research
• Principal Investigator: TAKADA Masahiko Tokyo Metropolitan Organization for Medical Research, 霊長類研究所, 教授 (00236233)
• Project Period (FY): 2005 – 2009
• Keywords: 神経科学 / 脳-神経 / 大脳基底核 / 大脳皮質 / ドーパミン / パーキンソン病遺伝子治療 / ウイルスベクター

Research Abstract

By means of retrograde transneuronal labeling technique with rabies virus, the structural basis for cerebral networks involved in execution and control of actions was analyzed in macaque monkeys. Particularly, the precise architectures of loop circuits linking the primary motor cortex and the basal ganglia/cerebellum, and parietotemporal-prefrontal-motor cortical networks were elucidated in this project. Moreover, basic approaches to gene therapy for Parkinson's disease were attempted to express functional molecules for protecting nigral dopamine neurons against degeneration/loss by gene transfer thereinto with recombinant viral vectors.Recruitment of the calcium-binding protein, calbindin, into the dopamine neurons successfully prevented the onset of parkinsonian insults in macaque monkeys treated withMPTP.

Research Products

• [Journal Article] Motor and nonmotor projections from the cerebellum to rostrocaudally distinct sectors of the dorsal premotor cortex in macaques. (2010)
  • Author(s): Hashimoto M, Takahara D, Hirata Y, Inoue K, Miyachi S, Nambu A, Tanji J, Takada M, Hoshi E.
  • Journal Title: European Journal of Neuroscience Vol.31 Pages: 1402-1413
  • Peer Reviewed
• [Journal Article] Expression of ErbB4 in substantia nigra dopamine neurons of monkeys and humans. (2009)
  • Author(s): Zheng Y, Watakabe A, Takada M, Kakita A, Namba H, Takahashi H, Yamamori T, Nawa H.
  • Journal Title: Progress in Neuro-Psychopharmacology & Biological Psychiatry Vol.33 Pages: 701-706
  • Peer Reviewed
• [Journal Article] Transdural Doppler ultrasonography monitors cerebral blood flow changes in relation to motor tasks. (2009)
  • Author(s): Hatanaka N, Tokuno H, Nambu A, Takada M.
  • Journal Title: Cerebral Cortex Vol.19 Pages: 820-831
  • Peer Reviewed
• [Journal Article] Motor cortical control of internal pallidal activity through glutamatergic and GABAergic inputs in awake monkeys. (2008)
  • Author(s): Tachibana Y, Kita H, Chiken S, Takada M, Nambu A.
  • Journal Title: European Journal of Neuroscience Vol.27 Pages: 238-253
  • Peer Reviewed
• [Journal Article] Efficient gene transfer via retrograde transport in rodent and primate brains by an HIV-1-based vector pseudotyped with rabies virus glycoprotein. (2007)
  • Author(s): Kato S, Inoue K, Kobayashi K, Yasoshima Y, Miyachi S, Inoue S, Hanawa H, Shimada T, Takada M, Kobayashi K.
  • Journal Title: Human Gene Therapy Vol.18 Pages: 1141-1151
  • Peer Reviewed
• [Journal Article] (2007)
  • Author(s): Kanamatsu T, Otuki T, Tokuno H, Nambu A, Takada M, Okamoto K, Watanabe H, Umeda M, Tsukada Y.
  • Journal Title: Brain Research Vol.1181 Pages: 142-148
  • Peer Reviewed
• [Journal Article] Temporal integration by stochastic recurrent network dynamics with bimodal neurons. (2007)
  • Author(s): Okamoto H, Isomura Y, Takada M, Fukai T.
  • Journal Title: Journal of Neurophysiology Vol.97 Pages: 3859-3867
  • Peer Reviewed
• [Journal Article] Layer and frequency dependences of phase response properties of pyramidal neurons in rat motor cortex. (2007)
  • Author(s): Tsubo Y, Takada M, Reyes A, Fukai T.
  • Journal Title: European Journal of Neuroscience Vol.25 Pages: 3429-3441
  • Peer Reviewed
• [Journal Article] Distinct types of ionic modulation of GABA actions in pyramidal cells and interneurons during electrical induction of hippocampal seizure-like network activity. (2007)
  • Author(s): Fujiwara-Tsukamoto Y, Isomura Y, Imanishi M, Fukai T, Takada M.
  • Journal Title: European Journal of Neuroscience Vol.25 Pages: 2713-2725
  • Peer Reviewed
• [Journal Article] Topographic distribution of output neurons in cerebellar nuclei and cortex to somatotopic map of primary motor cortex. (2007)
  • Author(s): Lu X, Miyachi S, Ito Y, Nambu A, Takada M.
  • Journal Title: European Journal of Neuroscience Vol.25 Pages: 2374-2382
  • Peer Reviewed
• [Journal Article] Differential characteristics of HIV-based vs. SIV-based lentiviral vector systems: gene delivery to neurons and axonal transport of expressed gene. (2007)
  • Author(s): Kitagawa R, Miyachi S, Hanawa H, Takada M, Shimada T.
  • Journal Title: Neuroscience Research Vol.57 Pages: 550-558
  • Peer Reviewed
• [Journal Article] Neuronal specificity of α-synuclein toxicity and effect of parkin co-expression in primates. (2007)
  • Author(s): Yasuda T, Miyachi S, Kitagawa R, Wada K, Nihira T, Ren Y-R, Hirai Y, Ageyama N, Terao K, Shimada T, Takada M, Mizuno Y, Mochizuki H.
  • Journal Title: Neuroscience Vol.144 Pages: 743-753
  • Peer Reviewed
• [Journal Article] Somatotopically arranged inputs from putamen and subthalamic nucleus to primary motor cortex. (2006)
  • Author(s): Miyachi S, Lu X, Imanishi M, Sawada K, Nambu A, Takada M.
  • Journal Title: Neuroscience Research Vol.56 Pages: 300-308
  • Peer Reviewed
• [Journal Article] Two opposing roles of 4-AP-sensitive K+ current in initiation and invasion of spikes in rat mesencephalic trigeminal neurons. (2006)
  • Author(s): Saito M, Murai Y, Bae Y, Akaike T, Takada M, Kang Y.
  • Journal Title: Journal of Neurophysiology Vol.96 Pages: 1887-1901
  • Peer Reviewed
• [Journal Article] Comparable GABAergic mechanisms of hippocampal seizure-like activity in post-tetanic and low-Mg2+ conditions. (2006)
  • Author(s): Fujiwara-Tsukamoto Y, Isomura Y, Takada M.
  • Journal Title: Journal of Neurophysiology Vol.95 Pages: 2013-2019
  • Peer Reviewed
• [Journal Article] Down-regulation of metabotropic glutamate receptor 1αin globus pallidus and substantia nigra of parkinsonian monkeys. (2005)
  • Author(s): Kaneda K, Tachibana Y, Imanishi M, Kita H, Shigemoto R, Nambu A, Takada M.
  • Journal Title: European Journal of Neuroscience Vol.22 Pages: 3241-3254
  • Peer Reviewed
• [Journal Article] Input□ output organization of jaw movement-related areas in monkey frontal cortex. (2005)
  • Author(s): Hatanaka N, Tokuno H, Nambu A, Inoue T, Takada M.
  • Journal Title: The Journal of Comparative Neurology Vol.492 Pages: 401-425
  • Peer Reviewed
• [Journal Article] Region-specific modulation of electrically induced synchronous oscillations in the rat hippocampus and cerebral cortex. (2005)
  • Author(s): Kaneda K, Fujiwara-Tsukamoto Y, Isomura Y, Takada M.
  • Journal Title: Neuroscience Research Vol.52 Pages: 83-94
  • Peer Reviewed
• [Journal Article] Organization of multisynaptic inputs from prefrontal cortex to primary motor cortex as revealed by retrograde transneuronal transport of rabies virus. (2005)
  • Author(s): Miyachi S, Lu X, Inoue S, Iwasaki T, Koike S, Nambu A, Takada M.
  • Journal Title: The Journal of Neuroscience Vol.25 Pages: 2547-2556
  • Peer Reviewed
• [Presentation] Organization of multisynaptic inputs to MT and V4 of macaques: Their hierarchic ranks and converging geniculate inputs from magno- and parvocellular layers. (2009)
  • Author(s): Ninomiya T, Sawamura H, Inoue K, Takada M.
  • Organizer: 39th Annual Meeting of Society for Neuroscience
  • Place of Presentation: Chicago
  • Year and Date: 2009-10-19
• [Book] 生体の科学(特集 脳科学のモデル実験動物, 61) (2010)
  • Author(s): 高田昌彦, 井上謙一
  • Total Pages: 53-58