Local neuronal circuit in the motor cortical areas

2009 Fiscal Year Final Research Report

• Project/Area Number: 17022020
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Kyoto University
• Principal Investigator: KANEKO Takeshi Kyoto University, 医学研究科, 教授 (90177519)
• Project Period (FY): 2005 – 2009
• Keywords: 神経科学 / 解剖学 / シグナル伝達 / 細胞・組織 / 生理学

Research Abstract

We developed a tool for the study of the local circuit of the motor cortical areas, and obtained the following results on the local circuit using the "From one to group" strategy:
(1)The local output of GABAergic cortical interneurons to corticospinal projection neurons (CSNs) was analysed, and found that each interneuron group differentially innervated CSNs.
(2)We developed BAC transgenic mice and rats expressing dendritic membrane targeted GFP under the specific control of parvalbumin (PV) promoter, and examined the excitatory and inhibitory inputs to PV-expressing interneurons.
(3) We developed adenoviral vector that enable Golgi-stain-like retrograde labeling of cortical neurons, and studied the local connections of pyramidal neurons to corticothalamic projection neurons.

Research Products

• [Journal Article] Expression of gap junction protein connexin36 in multiple subtypes of GABAergic neurons in adult rat somatosensory cortex. (2011)
  • Author(s): Ma Y, Hioki H, Konno M, Pan S, Nakamura H, Nakamura KC, Furuta T, Li J-L, Kaneko T.
  • Journal Title: Cerebral Cortex in press.
  • Peer Reviewed
• [Journal Article] Exclusive and common targets of neostriatofugal projections of rat striosome neurons : a single neuron-tracing study using a viral vector. (2011)
  • Author(s): Fujiyama F, Sohn J, Nakano T, Furuta T, Nakamura KC, Matsuda W, Kaneko T.
  • Journal Title: European Journal of Neuroscience vol.33 (no.4) Pages: 668-677
  • Peer Reviewed
• [Journal Article] Complementary distribution of glutamatergic cerebellar and GABAergic basal ganglia afferents to the rat motor thalamic nuclei. (2011)
  • Author(s): Kuramoto E, Fujiyama F, Nakamura KC, Tanaka Y, Hioki H, Kaneko T.
  • Journal Title: European Journal of Neuroscience vol.33 (no.1) Pages: 95-109
  • Peer Reviewed
• [Journal Article] Coexpression of VGLUT1 and VGLUT2 in trigeminothalamic projection neurons in the principal sensory trigeminal nucleus of the rat. (2010)
  • Author(s): Ge S-N, Ma Y-F, Hioki H, Wei Y-Y, Kaneko T, Mizuno N, Gao G-D, Li J-L.
  • Journal Title: The Journal of Comparative Neurology vol.518 (no.15) Pages: 3149-3168
  • Peer Reviewed
• [Journal Article] Ischemia-induced neurogenesis of neocortical layer 1 progenitor cells. (2010)
  • Author(s): Ohira K, Furuta T, Hioki H, Nakamura KC, Kuramoto E, Tanaka Y, Funatsu N, Shimizu K, Oishi T, Hayashi M, Miyakawa T, Kaneko T, Nakamura S.
  • Journal Title: Nature Neuroscience vol.13 (no.2) Pages: 173-179
  • Peer Reviewed
• [Journal Article] Vesicular glutamate transporter 3-expressing nonserotonergic projection neurons constitute a subregion in the rat midbrain raphe nuclei. (2010)
  • Author(s): Hioki H, Nakamura H, Ma Y-F, Konno M, Hayakawa T, Nakamura KC, Fujiyama F, Kaneko T.
  • Journal Title: The Journal of Comparative Neurology vol.518 (no.5) Pages: 668-686
  • Peer Reviewed
• [Journal Article] Afferent islands are larger than μ-opioid receptor patch in striatum of rat pups. (2009)
  • Author(s): Nakamura KC, Fujiyama F, Furua T, Hioki H, Kaneko T.
  • Journal Title: NeuroReport vol 20 (no 6) Pages: 584-588
  • Peer Reviewed
• [Journal Article] Single nigrostriatal dopaminergic neurons form widely spread and highly dense axonal arborizations in the neostriatum. (2009)
  • Author(s): Matsuda W, Furuta T, Nakamura KC, Hioki H, Fujiyama F, Arai R, Kaneko T.
  • Journal Title: The Journal of Neuroscience vol.29 (no.2) Pages: 444-453
  • Peer Reviewed
• [Journal Article] Two types of thalamocortical projections from the motor thalamic nuclei of the rat : A single neuron tracing study using viral vectors. (2009)
  • Author(s): Kuramoto E, Furuta T, Nakamura KC, Unzai T, Hioki H, Kaneko T.
  • Journal Title: Cerebral Cortex vol.19 (no.9) Pages: 2065-2077
  • Peer Reviewed
• [Journal Article] High-level transgene expression in neurons by lentivirus with Tet-Off system. (2009)
  • Author(s): Hioki H, Kuramoto E, Konno M, Kameda H, Takahashi Y, Nakano T, Nakamura KC, Kaneko T.
  • Journal Title: Neuroscience Research vol.63 (no.2) Pages: 149-154
  • Peer Reviewed
• [Journal Article] Recurrent infomax generates cell assemblies, neuronal avalanches, and simple cell-like selectivity. (2009)
  • Author(s): Tanaka T, Kaneko T, Aoyagi T
  • Journal Title: Neural Computation vol 21 (no 4) Pages: 1038-1067
  • Peer Reviewed
• [Journal Article] Paucity of enkephalin production in neostriatal striosomal neurons : Analysis with preproenkephalin/green fluorescent protein transgenic mice. (2008)
  • Author(s): Koshimizu Y, Wu S-H, Unzai T, Hioki H, Sonomura T, Nakamura KC, Fumino Fujiyama F, Kaneko T
  • Journal Title: European Journal of Neuroscience vol.28 (no.10) Pages: 2053-2064
  • Peer Reviewed
• [Journal Article] Production and histological application of affinity-purified antibodies to heat-denatured green fluorescent protein. (2008)
  • Author(s): Nakamura KC, Kameda H, Koshimizu Y, Yanagawa Y, Kaneko T
  • Journal Title: Journal of Histochemistry & Cytochemistry vol.56 (no.7) Pages: 647-657
  • Peer Reviewed
• [Journal Article] The Effects of cutting solutions on the viability of GABAergic interneurons in cerebral cortical slices of adult mice. (2008)
  • Author(s): Tanaka Yasuyo, Tanaka Yasuhiro, Furuta T, Yanagawa Y, Kaneko T
  • Journal Title: Journal of Neuroscience Methods vol.171 (no.1) Pages: 118-125
  • Peer Reviewed
• [Journal Article] Targeting green fluorescent protein to dendritic membrane in central neurons. (2008)
  • Author(s): Kameda H, Furuta T, Matsuda W, Ohira K, Nakamura Ko, Hioki H, Kaneko T
  • Journal Title: Neuroscience Research vol.61 (no.1) Pages: 79-91
  • Peer Reviewed
• [Journal Article] Some gamma-motoneurons contain gamma-aminobutyric acid in the rat cervical spinal cord. (2008)
  • Author(s): Ito T.Hioki H, Nakamura Ko, Kaneko T, Iino S, Nojyo Y
  • Journal Title: Brain Research vol.1201 Pages: 78-87
  • Peer Reviewed
• [Journal Article] Transiently increased colocalization of vesicular glutamate transporters 1 and 2 at single axon terminals during postnatal development of mouse neocortex : a quantitative analysis with correlation coefficient. (2007)
  • Author(s): Nakamura Ko, Watakabe A, Hioki H, Fujiyama F, Tanaka Y, Yamamori T, Kaneko T
  • Journal Title: European Journal of Neuroscience vol.26 (no.8) Pages: 3054-3067
  • Peer Reviewed
• [Journal Article] Efficient gene transduction of neurons by lentivirus with enhanced neuron-specific promoters. (2007)
  • Author(s): Hioki H, Kameda H, Nakamura H, Okunomiya T, Ohira K, Nakamura Ko, Kuroda M, Furuta T, Kaneko T
  • Journal Title: Gene Therapy vol.14 (no.11) Pages: 872-882
  • Peer Reviewed
• [Journal Article] GABA-containing sympathetic preganglionic neurons in rat thoracic spinal cord send their axons to the superior cervical ganglion. (2007)
  • Author(s): Ito T, Hioki H, Nakamura Ko, Tanaka Y, Nakade H, Kaneko T, Iino S, Nojyo Y
  • Journal Title: The Journal of Comparative Neurology vol.502 (no.1) Pages: 113-125
  • Peer Reviewed
• [Journal Article] Truncated TrkB-T1 regulates the morphology of neocortical layer I astrocytes in adult rat brain slices. (2007)
  • Author(s): Ohira K, Funatsu N, Homma KJ, Sahara Y, Hayashi M, Kaneko T, Nakamura S
  • Journal Title: European Journal of Neuroscience vol.25 (no.2) Pages: 406-416
  • Peer Reviewed
• [Journal Article] Metabotropic glutamate receptor 4-immunopositive terminals of medium-sized spiny neurons selectively form synapses with cholinergic interneurons in the rat neostriatum. (2007)
  • Author(s): Kuramoto E, Fujiyama F, Unzai T, Nakamura Ko, Hioki H, Furuta T, Shigemoto R, Ferraguti F, Kaneko T
  • Journal Title: The Journal of Comparative Neurology vol.500 (no.5) Pages: 908-922
  • Peer Reviewed
• [Journal Article] Medullary dorsal horn neurons providing axons both to the parabrachial nucleus and the thalamus. (2006)
  • Author(s): Li J-L, Xiong K, Pang Y-W, Dong Y, Kaneko T, Mizuno N
  • Journal Title: The Journal of Comparative Neurology vol.498 (no.4) Pages: 539-551
  • Peer Reviewed
• [Journal Article] Expression of vesicular glutamate transporter VGLUT1 immuno- reactivity in peripheral and central endings of trigeminal mesencephalic nucleus neurons in the rat. (2006)
  • Author(s): Pang Y-W, Li J-L, Nakamura Ko, Wu S, Kaneko T, Mizuno N
  • Journal Title: The Journal of Comparative Neurology vol.498 (no.1) Pages: 129-141
  • Peer Reviewed
• [Journal Article] Direct pyrogenic input from prostaglandin EP3 receptor-expressing preoptic neurons to the dorsomedial hypothalamus. (2005)
  • Author(s): Nakamura Y, Nakamura Ka, Matsumura K, Kobayashi S, Kaneko T, Morrison SF
  • Journal Title: European Journal of Neuroscience vol.22 (no.12) Pages: 3137-3146
  • Peer Reviewed
• [Journal Article] Postnatal changes of vesicular glutamate transpoter 1 (VGluT1) and VGluT2 and their colocalization in the mouse forebrain. (2005)
  • Author(s): Nakamura Ko., Hioki H., Fujiyama F., Kaneko T.
  • Journal Title: The Journal of Comparative Neurology vol.492 (no.3) Pages: 263-288
  • Peer Reviewed
• [Presentation] S13-4シンポジウム;「皮質視床投射神経細胞への皮質内入力に現れるサブカラム構造」 (2011)
  • Author(s): 田中康裕, 田中康代, 今野美知輝, 藤山文乃, 岡本-古田敬子, 薗村貴弘, 亀田浩司, 日置寛之, 古田貴寛, 中村公一, 金子武嗣
  • Organizer: 第116回日本解剖学会・全国学術集会
  • Place of Presentation: 横浜
  • Year and Date: 20110328-20110330
• [Presentation] Single-neuron tracing study of thalamocortical projections arising from the rat ventral medial nucleus by using viral vectors. (2010)
  • Author(s): KURAMOTO E, FUJIYAMA F, FURUTA T, UNZAI T, HIOKI H, TANAKA Y, KANEKO T
  • Organizer: The 40th Society for Neuroscience Annual Meeting
  • Place of Presentation: San Diego, CA, USA.
  • Year and Date: 20101113-20101117
• [Presentation] Subcolumnar structures in local inputs of pyramidal neurons onto corticothalamic neurons in rat barrel cortex. (2010)
  • Author(s): TANAKA Yasuhiro、TANAKA Yasuyo, KONNO M, FUJIYAMA F, OKAMOTO- FURUTA K, SONOMURA T, KAMEDA H, HIOKI H, FURUTA T, NAKAMURA KC, KANEKO T.
  • Organizer: The 40th Society for Neuroscience Annual Meeting
  • Place of Presentation: San Diego, CA, USA.
  • Year and Date: 20101113-20101117
• [Presentation] Transgenic approaches to dendritic functions of cortical neurons. (2009)
  • Author(s): Kaneko T
  • Organizer: Kyoto University International Symposium 'Cellular Approaches to Neuronal Signal Processing'
  • Place of Presentation: Kyoto.
  • Year and Date: 20090623-20090624
• [Presentation] 三叉神経核群の構造と機能:中間亜核に注目 して.In【S05神経解剖学懇話会シンポジウ ム】最近の神経回路網解明の進歩 (2009)
  • Author(s): 古田貴寛, Martin Deschenes, 金子武嗣
  • Organizer: 第114回日本解剖学会・全国学術集会
  • Place of Presentation: 岡山
  • Year and Date: 20090327-20090329
• [Presentation] Local circuit in the motor cortex. (2005)
  • Author(s): Kaneko T, Cho R-H
  • Organizer: In : Symposium 'Local Circuit in the cerebral cortex, hippocampus and thalamus', The 28th Annual Meeting of the Japan Neuroscience Society
  • Place of Presentation: Yokohama.
  • Year and Date: 20050726-20050728
• [Presentation] Neural Circuits Involving Patch and Matrix Compartments of Rat Neostriatum.In Symposium SY2A-B1.
  • Author(s): Fujiyama F, Sohn J, Nakano T, Unzai T, Koshimizu Y, Kaneko T
  • Organizer: The 31st Annual Meeting of the Japan Neuroscience Society
  • Place of Presentation: Tokyo.
  • Year and Date: 00000709-00000711
• [Book] 神経組織の構造研究(外山敬介 編集)(In : ノーベル賞の生命科学入門 脳と神経のはたらき)(第2章) (2010)
  • Author(s): 金子武嗣
  • Total Pages: 22-35
  • Publisher: 講談社
• [Remarks] ホームページ等
  • URL: http://www.mbs.med.kyoto-u.ac.jp