A study of searching the growth cone proteins involved in synapse formation

2009 Fiscal Year Final Research Report

• Project/Area Number: 17023019
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Niigata University
• Principal Investigator: IGARASHI Michihiro Niigata University, 医歯学系, 教授 (50193173)
• Co-Investigator(Kenkyū-buntansha): TANAKA Kennichi 新潟大学, 医歯学系, 教授 (10126427) ; ABE Haruki 新潟大学, 医歯学系, 教授 (40018875) ; FUJII Hiroshi 信州大学, 農学部, 教授 (90165340) ; TAOKA Masato 首都大学, 東京大学院・理学(系)研究科, 助教 (60271160) ; WATANABE Michitoshi 新潟大学, 医歯学系, 助教 (40303127) ; NOZUMI Motohiro 新潟大学, 医歯学系, 助教 (00420323)
• Project Period (FY): 2005 – 2009
• Keywords: 成長円錐 / プロテオミクス / RNAi / 神経極性形成 / リン酸化 / コンドロイチン硫酸 / シンタキシン / 遺伝子改変マウス

Research Abstract

The growth cone is the essential structure for accurate synaptogenesis to form the brain neuronal network, however, in the mammalian brain, the molecular basis is poorly understood. Here, we successfully used a proteomic approach to identify 945 proteins, including highly abundant, membrane-associated and actin-associated proteins. Almost one hundred of the proteins appear to be highly enriched in the growth cone, as determined by quantitative immunostaining, and for 18 proteins, the results of RNAi suggest a role in axon growth. Most of the proteins we identified have not previously been implicated in axon growth and thus, their identification presents a significant first step forward, providing new marker proteins and candidate neuronal growth-associated proteins.

Research Products

• [Journal Article] Identification of functional marker proteins in the mammalian growth cone (2009)
  • Author(s): Nozumi, M., Togano, T., Takahashi-Niki, K., Lu, J., Honda, A., Taoka, M., Shinkawa, T., Koga, H., Takeuchi, K., Isobe, T., Igarashi M
  • Journal Title: Proc Natl Acad Sci USA 106 Pages: 17211-17216
  • Peer Reviewed
• [Journal Article] A novel method for RNA interference using EGFP-transgenic rats (2008)
  • Author(s): Lu, J., Nozumi, M., Fujii, H., Igarashi, M.
  • Journal Title: Neurosci. Res 61 Pages: 219-224
  • Peer Reviewed
• [Journal Article] Drebrin attenuates the interaction between actin and myosin-V. Biochem (2007)
  • Author(s): Ishikawa, R., Katoh, K., Takahashi, A., Ooseki, K., Watanabe, M., Igarashi M, Nakamura, A., Kohama, K.
  • Journal Title: Biophys. Res. Commun 359 Pages: 398-401
  • Peer Reviewed
• [Journal Article] Roles of calmodulin-binding proteins in synaptic vesicle recycling during regulated exocytosis at submicromolar Ca2+ concentrations (2007)
  • Author(s): Igarashi, M., Watanabe, M.
  • Journal Title: Neurosci. Res 58 Pages: 226-233
  • Peer Reviewed
• [Journal Article] Myosin-Va regulates exocytosis through Ca2+-dependent binding of syntaxin-1A (2005)
  • Author(s): Watanabe, M., Nomura, K., Ohyama, A., Ishikawa, R., Komiya, Y., Hosaka, Y., Yamauchi, E., Taniguchi, H., Sasakawa, N., Ushiki, T,. Sato, O., Kumakura, K., Ikebe, M., Igarashi M
  • Journal Title: Mol. Biol. Cell 16 Pages: 4519-4530
  • Peer Reviewed
• [Journal Article] Role of Ser 50 phosphorylation in SCG10 regulation of microtubule depolymerizaiton (2005)
  • Author(s): Togano, T., Kurachi, M., Watanabe, M., Grenningloh, G., Igarashi, M
  • Journal Title: J. Neurosci. Res 80 Pages: 475-480
  • Peer Reviewed
• [Presentation] Functional Marker Proteins in the Mammalian Growth Cone (2009)
  • Author(s): garashi, M., Toagano, T., Nozumi, M.
  • Organizer: 17023019seika. pdf49th American Society for Cell Biology
  • Place of Presentation: San Diego, USA
  • Year and Date: 2009-12-07
• [Presentation] プロテオミクスによって初めて可能となった成長円錐研究:特異分子マーカーと機能制御機構 (2009)
  • Author(s): 五十嵐道弘
  • Organizer: 第5回プロテオミクス・構造生物学講演会
  • Place of Presentation: 東京
  • Year and Date: 2009-11-02
• [Presentation] Newly identified neuronal growth-associated proteins (nGAPs) are involved in rearrangement of cytoskeletons in the growth cone (2009)
  • Author(s): Nozumi, M., Igarashi, M.
  • Organizer: 32nd Society for Neuroscience Annual Meeting
  • Place of Presentation: Chicago, USA
  • Year and Date: 2009-10-17
• [Book] Proteins Involved in the Presynaptic Functions.(Lajtha A, Mikoshiba K, eds, Handbook of Neurochemistry and Molecular Neurobiology: Neural Signaling Mechanisms) (2009)
  • Author(s): Igarashi, M., Ohko, K.
  • Total Pages: 45-62
  • Publisher: Springer
• [Remarks]
  • URL: http://www.med.niigata-u.ac.jp/bc2/