2009 Fiscal Year Final Research Report
Role of neuronal migration for the formation of neuronal connections
| Project/Area Number |
17023028
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| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | Osaka University |
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Principal Investigator |
MURAKAMI Fujio Osaka University, 大学院・生命機能研究科, 教授 (20089882)
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| Co-Investigator(Kenkyū-buntansha) |
SHIOI Go 理化学研究所, 発生再生科学総合研究センター, 研究員 (60391968)
TANABE Yasuto 大阪大学, 大学院・生命機能研究科, 准教授 (10311309)
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| Project Period (FY) |
2005 – 2009
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| Keywords | ラディアル線維 / 下菱脳唇 / 小脳前核細胞 / 接線方向移動 / 放射状方向移動 / 神経回路形成 / 神経核 / 細胞移動 |
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| Research Abstract |
The development of mossy-fibre projecting precerebellar neurons (PCN) presents a classical example of tangential neuronal migration. Among the PCN, the pontine neurons follow a stereotypic anteroventral-directed pathway to form the pontine nuclei in the pons. The guidance mechanisms that determine the marginal migration of PCN and the anterior migration of pontine neurons are poorly understood. Here, we report that a chemokine SDF1 derived from the meningeal tissue regulates the migratory pathways of PCN. PCN are chemoattracted by the meningeal tissue, an effect that is mimicked by an SDF1 source. Analysis of knockout mice for the Sdf1 receptor Cxcr4 shows that both the marginal migration of PCN and the anterior migration of pontine neurons are disrupted. We further find that SDF1/CXCR4 signalling regulates these two processes cell-autonomously. As a result of disrupted neuronal migration, pontine nuclei formation was highly abnormal, with the presence of multiple ectopic pontine clusters posteriorly. The ectopic pontine clusters led to ectopic collateral branch formation from the corticospinal tract. Our results together demonstrate crucial roles for SDF1/CXCR4 in multiple aspects of PCN migration and highlight the deleterious consequence of derailed migration on proper nuclei formation. Furthermore, we provide the first in vivo evidence that pontine neurons themselves induce collateral branching from the corticospinal axons.
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[Journal Article] Distinct roles of neuropilin 1 signaling for radial and tangential extension of callosal axons.2009
Author(s)
Hatanaka,Y, Matsumoto,T, Yanagawa,Y, Fujisawa,H, Murakami,F, Masu,M
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Journal Title
J Comp Neurol. 20;514(3)
Pages: 215-25
Peer Reviewed
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[Journal Article] Random walk behavior of migrating cortical interneurons in the marginal zone : time-lapse analysis in flat-mount cortex.2009
Author(s)
Tanaka,DH, Yanagida,M, Zhu,Y, Mikami,S, Nagasawa,T, Miyazaki,J, Yanagawa,Y, Obata,K, Murakami,F
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Journal Title
J Neurosci. 4;29(5)
Pages: 1300-1311
Peer Reviewed
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[Journal Article] Crucial roles of Robo proteins in midline crossing of cerebellofugal axons and lack of their up-regulation after midline crossing.2008
Author(s)
Tamada,A, Kumada,T, Zhu,Y, Matsumoto,T, Hatanaka,Y, Muguruma,K, Chen,Z, Tanabe,Y, Torigoe,M, Yamauchi,K, Oyama,H, Nishida,K, Murakami,F
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Journal Title
Peer Reviewed
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[Journal Article] The role of Slit-Robo signaling in the generation, migration and morphological differentiation of cortical interneurons.Epub2008
Author(s)
Andrews,W, Barber,M, Hernadez-Miranda LR, Xian,J, Rakic,S, Sundaresan,V, Rabbitts,TH, Pannell,R, Rabbitts,P, Thompson,H, Erskine,L, Murakami,F, Parnavelas,JG
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Journal Title
Dev Biol. 15;313(2)
Pages: 648-58
Peer Reviewed
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[Journal Article] Robo1 regulates the development of major axon tracts and interneuron migration in the forebrain.2006
Author(s)
Andrews,W, Liapi,A, Plachez,C, Camurri,L, Zhang,J, Mori,S, Murakami,F, Parnavelas,JG, Sundaresan,V, Richards,LJ
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Journal Title
Development. 133(11)
Pages: 2243-2252
Peer Reviewed
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[Journal Article] Direct visualization of nucleogenesis by precerebellar neurons : involvement of ventricle-directed, radial fibre-associated migration.2006
Author(s)
Kawauchi,D, Taniguchi,H, Watanabe,H, Saito,T, Murakami,F
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Journal Title
Development. 133(6)
Pages: 1113-1123
Peer Reviewed
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