2009 Fiscal Year Final Research Report
Mechanism for regulation of DECODE complexes in gene activation
| Project/Area Number |
17054033
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| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | Yokohama City University |
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Principal Investigator |
OGATA Kazuhiro Yokohama City University, 医学研究科, 教授 (90260330)
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| Co-Investigator(Renkei-kenkyūsha) |
SHIINA Masaaki 横浜市立大学, 医学部, 助教 (30347299)
HAMADA Keisuke 横浜市立大学, 医学部, 助教 (00344052)
SATO Ko 横浜市立大学, 医学部, 助教 (90300962)
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| Project Period (FY) |
2005 – 2009
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| Keywords | 転写因子 / タンパク質-核酸複合体 / Ets1,Runx1,エンハンサー / 化学修飾 / 連携研究者 |
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| Research Abstract |
Multiple transcription factors (TFs) regulate transcription by forming TFs-DNA complexes. A cell signaling causes chemical modification of transcription factors, which modulates transcriptional activities. Besides, effect of chemical modification of transcription factors on their activity in the context of TFs-DNA complexes, however, is largely unknown. In this sturdy, we showed that the phosphorylation of a transcription factor Ets1 "differentially" modulates Ets1-containing TFs-DNA complexes. In addition, we clarified the underlying mechanism by which the selective modulation of Ets1-containing TFs-DNA complexes is achieved using NMR analyses of allosteric regulation of Runx1 by CBFβ and crystallographic analyses of multiprotein-DNA complex comprised of Ets1, Runx1 and CBFβ formed on the TCRα gene enhancer.
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Research Products
(32 results)
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[Journal Article] Loss-of-function mutations of CHST14 in a new type of Ehlers-Danlos syndrome2010
Author(s)
Miyake N, Kosho T, Mizumoto S, Furuichi T, Hatamochi A, Nagashima Y, Arai E, Takahashi K, Kawamura R, Wakui K, Takahashi J, Kato H, Yasui H, Ishida T, Ohashi H, Nishimura G, Shiina M, Saitsu H, Tsurusaki Y, Doi H, Fukushima Y, Ikegawa S, Yamada S, Sugahara K, Matsumoto N
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Journal Title
Hum Mutat 31(8)
Pages: 966-974
Peer Reviewed
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[Journal Article] Dominant-negative mutations in alpha-II spectrin cause West syndrome with severe cerebral hypomyelination, spastic quadriplegia, and developmental delay2010
Author(s)
Saitsu H, Tohyama J, Kumada T, Egawa K, Hamada K, Okada I, Mizuguchi T, Osaka H, Miyata R, Furukawa T, Haginoya K, Hoshino H, Goto T, Hachiya Y, Yamagata T, Saitoh S, Nagai T, Nishiyama K, Nishimura A, Miyake N, Komada M, Hayashi K, Hirai S, OgOgS, OggS, Oggg, Oga K, Kato M, Fukuda A, Matsumoto N
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Journal Title
Am J Hum Genet 86(6)
Pages: 881-891
Peer Reviewed
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[Journal Article] Rational approach to the synthesis, evaluation and 68Ga labeling of a novel 4-anilinoquinoline EGFR inhibitor as a new imaging agent that selectively targets the EGFR tyrosine kinase2010
Author(s)
Theeraladanon C, Takahashi N, Shiina M, Hamada K, Takada Y, Endo H, Tateishi U, Oka T, Ogata K, Inoue T
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Journal Title
Cancer Biotherapy & Radiopharmaceuticals 25(4)
Pages: 479-485
Peer Reviewed
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[Journal Article] De novo mutations in the gene encoding STXBP1(MUNC18-1)cause early infantile epileptic encephalopathy2008
Author(s)
Saitsu H, Kato M, Mizuguchi T, Hamada K, Osaka H, Tohyama J, Uruno K, Kumada S, Nishiyama K, Nishimura A, Okada I, Yoshimura Y, Hirai S, Kumada T, Hayasaka K, Fukuda A, Ogata K, Matsumoto N
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Journal Title
Nat Genet 40(6)
Pages: 782-788
Peer Reviewed
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