生体シグナル解析用分子材料群の創製

2008 Fiscal Year Annual Research Report

• Project/Area Number: 17066005
• Research Institution: Kyoto University
• Principal Investigator: 浜地 格 Kyoto University, 工学研究科, 教授 (90202259)
• Co-Investigator(Kenkyū-buntansha): 三原 久和 東京工業大学, 生命理工学研究科, 教授 (30183966) ; 杉本 直己 甲南大学, 理工学部, 教授 (60206430) ; 養王田 正文 東京農工大学, 共生科学技術研究部, 教授 (50250105) ; 金原 数 東北大学, 多元物質科学研究所, 准教授 (30282578)
• Keywords: バイオセンサー / DNA構造変化 / 分子クラウディング / ペプチドアレイ / 化合物ライブラリー / 分子認識 / 化学センサー / 機能性タンパク質

Research Abstract

A01班では、新しい人工分子や機能性材料の定量的パラメーターを用いた分子設計による合成と開発, その特性の解析と生体シグナル解析への利用というアプローチで多様な細胞内シグナルとなる物質群の網羅的解析に必要となる機能性分子群の創製を遂行している。その中で、浜地は、平成18年度までに成功したリン酸化タンパク質結合性の人工合成プローブを基体として細胞内でも使用できるATPに対する蛍光センサーの開発に成功した。また超分子ヒドロゲルとナノ細孔を有するMCMをハイブリッドしたアニオン性多糖類センサーチップの構築に成功した。杉本は、細胞内環境因子が核酸の構造安定性に及ぼす影響を定量的に検討し、水和状態によって核酸の構造と熱力学的安定性が劇的に変化することを見出した。この知見をもとにして、分子クラウディグ、金属イオン、代謝産物、光などに応答して細胞の機能を制御できる核酸スイッチを開発した。三原は、ペプチドチップの創製において、ヘリックス構造ペプチドライブラリを用いた細胞挿入活性リガンドのスクリーニング系の構築に成功した。さらに金の異常反射を利用したラベルフリー検出システムの構築にも高感度化を達成した。養王田はThermococcus由来プレフォルディンのα1β1ヘキサマー、β2テトラマーのX線結晶構造を解析した。また、α2β2ヘキサマーの結晶を得ることにも成功し、X線回折データも取得し、構造解析を行っている。これらは分子ツール開発の基礎データになる。また、GPCR受容体結合ペプチド配列を導入したプレフォルディンの特性解析を行った。金原は生体分子を超分子化学的に修飾する「分子糊」を開発し、これを用いて微小管の固定化に成功した。また、筒状タンパク質であるシャペロニンの化学修飾により、これらが直線状に連結したチューブ状ナノ構造体の形成に成功した。さらに、このナノチューブ内に変性タンパク質をはじめとするゲストを導入できることを見いだした。

Research Products

• [Journal Article] Molecular Recognition, Fluorescence Sensing, and Biological Assay of Phosphate Anion Derivatives Using Artificial Zn(II)-Dpa Complexes (2009)
  • Author(s): T. Sakamoto, A. Ojida, I. Hamachi
  • Journal Title: Chem. Commun. Feature Article Pages: 141-152
  • Peer Reviewed
• [Journal Article] MCM-Enzyme-Supramolecular Hydrogel Hybrid as a Fluorescence Sensing Material for Poly-anions of Biological Significance (2009)
  • Author(s): A. Wada, S. Tamaru, M. Ikeda, I. Hamachi
  • Journal Title: J. Am. Chem. Soc 131
  • Peer Reviewed
• [Journal Article] Ligand-directed tosyl chemistry for protein labeling in vivo (2009)
  • Author(s): S. Tsukiji, M. Miyagawa, Y. Takaoka, T. Tamura, I. Hamachi
  • Journal Title: Nature Chemical Biology 5
  • Peer Reviewed
• [Journal Article] Fluorescent BODIPY-Based Zn(II)-Complex as a Molecular Probe for Selective Detection of Neurofibrillary Tangles in the Brains of Alzheimer's Disease Patients (2009)
  • Author(s): A., Ojida, T. Sakamoto, M. Inoue, S. Fujishima, G. Lippens, I. Hamachi
  • Journal Title: J. Am. Chem. Soc.
  • Peer Reviewed
• [Journal Article] Selection and structural analysis of de novo proteins from an alpha3beta3 genetic library (2009)
  • Author(s): M. T. Jumawid, T. Takahashi, T. Yamazaki, H. Ashigai, H. Mihara
  • Journal Title: Protein Science 18 Pages: 384-398
  • Peer Reviewed
• [Journal Article] Poly(amidoamine)dendrimer-modified gold surfaces for anomalous reflection of gold (AR) method to detect biomolecular interactions (2009)
  • Author(s): A. Syahir, K. Tomizaki, K. Kajikawa, H. Mihara
  • Journal Title: Langmuir 25 Pages: 3667-3674
  • Peer Reviewed
• [Journal Article] Hydration of Watson-Crick base pairs and dehydration of Hoogsteen base pairs inducing structural polymorphism under molecular crowding conditions (2009)
  • Author(s): D. Miyoshi, K. Nakamura, H. Karimata, T. Ohmichi, N. Sugimoto
  • Journal Title: J. Am. Chem. Soc. 130 Pages: 3522-3531
  • Peer Reviewed
• [Journal Article] Reversible stability switching of a hairpin DNA via a photo-responsive linker unit (2009)
  • Author(s): L. Wu, K. Koumoto, N. Sugimoto
  • Journal Title: Chem. Commun Pages: 1915-1917
  • Peer Reviewed
• [Journal Article] Molecularly imprinted nanocomposites for highly sensitive SPR detection of a non-aqueous atrazine sample (2009)
  • Author(s): J. Matsui, M. Takayose, K. Akamatsu, H. Nawafune, K. Tamaki, N. Sugimoto
  • Journal Title: Analyst 134 Pages: 80-86
  • Peer Reviewed
• [Journal Article] Designable DNA Functions toward New Nanobiotechnology (2009)
  • Author(s): N. Sugimoto
  • Journal Title: sull. Chem. Coc. Jpn. 82 Pages: 1-10
  • Peer Reviewed
• [Journal Article] 「核酸アプタマー」のケミカル・ルネサンス"知る"から"活かす"への生命化学の新しい発想 (2009)
  • Author(s): 〓原正靖・杉本直己
  • Journal Title: 化学 64 Pages: 44-49
• [Journal Article] RNAスイッチを用いた微生物のタンパク質合成技術の開発と応用 (2009)
  • Author(s): 山内隆寛・杉本直己
  • Journal Title: バイオインダストリー 82 Pages: 66-72
• [Journal Article] An approach to peptide-based ATP receptors by a combination of random selection, rational design, and molecular imprinting (2009)
  • Author(s): J. Matsui, J. Nagano, D. Miyoshi, K. Tamaki, N. Sugimoto
  • Journal Title: Biosen. Bioelectron
  • Peer Reviewed
• [Journal Article] Characterization of a sHsp of Schizosaccharomyces pombe, SpHsp15.8, and the implication of its functional mechanism by comparison with another sHsp, SpHsp16.0 (2009)
  • Author(s): Sugino, C., Hirose, M., Tohda, H., Yoshinari, Y., Abe, T., Giga-Hama, Y., Iizukai, R., Shimizu, M., Kidokoro, S., Ishii, N. Yohda, M.
  • Journal Title: Proteins 74 Pages: 6-17
  • Peer Reviewed
• [Journal Article] Crystal structure of PH1733, an aspartate racemase homologue, from Pyrococcus horikoshii OT3 (2009)
  • Author(s): Kita, A., Tasaki, S., Yohda, M. Miki, K.
  • Journal Title: Protains 74 Pages: 240-244
  • Peer Reviewed
• [Journal Article] Molecular Glues Carrying Multiple Guanidinium Ion Pendants via an Oligoether Spacer : Stabilization of Microtubules against Depolymerization (2009)
  • Author(s): Kou Okuro, Kazushi Kinbara, et.al.
  • Journal Title: Journal of the Amerincan Chemical Society 131 Pages: 1626-1627
  • Peer Reviewed
• [Journal Article] Template Sol-Gel Synthesis of Mesostructured Silica Composite, Using Metal Complexes Bearing Amphiphilic Side Chains : Immobilization of a Polythene Pd Complex Formed by a Metallophilic Interaction (2009)
  • Author(s): Wataru Otani, Kazushi Kinbara, and Takuzo Aida
  • Journal Title: Faraday Discusssions 143
  • Peer Reviewed
• [Journal Article] Photo gel-sol/sol-gel transition and its patterning of a supramolecular hydrogel as stimuli-respnsive biomaterials (2008)
  • Author(s): S. Matsumoto, S. Yamaguchi, S. Ueno, H. Komatsu, M. Ikeda, K. Ishizuka, Y. Iko, K. V. Tabata, H. Aoki, S. Ito, H. Noii, I. Hamachi
  • Journal Title: Chem. -A Euro. J. 14 Pages: 3977-3986
  • Peer Reviewed
• [Journal Article] Mechanical reinforcement of supramolecular hydrogel comprising artificial glyco-lipid through supramolecular copolymerization (2008)
  • Author(s): M. Ikeda, Y. Shimizu, S. Matsumoto, H. Komatsu, S. Tamaru, T. Takigawa, I. Hamachi
  • Journal Title: Macromolecular Bioscience 8 Pages: 1019-1025
  • Peer Reviewed
• [Journal Article] Turn-on Fluorescence Sensing of Nucleside Polyphosphates Using a Xanthene-Based Zn(II) Complex Chemosensor (2008)
  • Author(s): A. Ojida, I. Takashima, T, Kohira, H. Nonaka, I. Hamachi
  • Journal Title: J. Am. Chem. Soc. 130 Pages: 12095-12101
  • Peer Reviewed
• [Journal Article] Three-dimensional encapsulation of live cells by using a hybrid matrix of nanoparticles in a supramolecular hydrogel (2008)
  • Author(s): M. Ikeda, S. Ueno, S. Matsumoto, Y. Shimizu, H. Komatsu, K. Kusumoto, I. Hamachi
  • Journal Title: Chem. -A Euro. J. 14 Pages: 10808-10815
  • Peer Reviewed
• [Journal Article] Fluorescence Imaging of Intracellular Cadmium Using a Dualexcitation Ratiometric Chemosensor (2008)
  • Author(s): M. Taki, M. Desaki, A. Ojida, T. Hirayama, I. Hamachi, Y. Yamamoto, S. Iyoshi
  • Journal Title: J. Am. Chem. Soc. 130 Pages: 12564-12565
  • Peer Reviewed
• [Journal Article] Real-time Off/On-mode Fluorescence Assay for Enzyme Reactions Involving Nucleoside Polyphosphates by Use of a Xanthene Zn(II)-Dpa Chemosensor (2008)
  • Author(s): T. Kohira, I. Takashima, H. Nonaka, A. Ojida, I. Hamachi
  • Journal Title: Chem. Lett. 37 Pages: 1164-1165
  • Peer Reviewed
• [Journal Article] Construction of Multi-functional Extracellular Matrix Proteins that Promote Tube Formation of Endothelial Cells (2008)
  • Author(s): M. Nakamura, M. Mie, H. Mihara, M. Nakamura, E. Kobatake
  • Journal Title: Biomaterials 29 Pages: 2977-2986
  • Peer Reviewed
• [Journal Article] Peptide and Protein Mimetics Inhibiting the Amyloid beta-Peptide Aggregation (2008)
  • Author(s): T. Takahashi, H. Mihara
  • Journal Title: Accounts of Chemical Research 41(10) Pages: 1309-1318
  • Peer Reviewed
• [Journal Article] Telomerase activity regulated by thermodynamic stability of DNA-RNA hybrid (2008)
  • Author(s): H. Yu, D. Zhang, X. Gu, D. Miyoshi, N. Sugimoto
  • Journal Title: Angew. Chem. Int. Ed. 47 Pages: 9034-9038
  • Peer Reviewed
• [Journal Article] Riboswitches for enhancing target gene expression in eukaryotes (2008)
  • Author(s): T. Yamauchi, D. Miyoshi, T. Kubodera, M. Ban, A. Nishimura, N. Sugimoto
  • Journal Title: Chem Bio Chem 9 Pages: 1040-1043
  • Peer Reviewed
• [Journal Article] Recognition of a flipped base in a hairpinloop DNA by a small peptide (2008)
  • Author(s): J. Kawakami, S. Okabe, Y. Tanabe, N. Sugimoto
  • Journal Title: Nucleosides, Nucleotides, and Nucleic Acids 27 Pages: 292-308
  • Peer Reviewed
• [Journal Article] Conformation and the sodium ion condensation on DNA and RNA structures in the presence of a neutral cosolute as a mimic of the intracellular media (2008)
  • Author(s): S. Nakano, L. Wu, H. Oka, H. T. Karimata, T. Kirihata, Y. Sato, S. Fujii, H. Sakai, M. Kuwahara, H. Sawai, N. Sugimoto
  • Journal Title: Mol. BioSys t. (Emerging Investigators issue) 4 Pages: 579-588
  • Peer Reviewed
• [Journal Article] Molecular crowding effects on structure and stability of DNA (2008)
  • Author(s): D. Miyoshi, N. Sugimoto
  • Journal Title: Biochimie 90 Pages: 1040-1051
  • Peer Reviewed
• [Journal Article] Design of allele-specific primers and detection of the human ABO genotyping to avoid the pseudopositive problem (2008)
  • Author(s): H. Yaku, T. Yukimasa, S. Nakano, N. Sugimoto, H. Oka
  • Journal Title: Electrophoresis 29 Pages: 4130-4140
  • Peer Reviewed
• [Journal Article] Molecular crowding effect on metal ion binding properties of the hammerhead ribozyme (2008)
  • Author(s): S. Nakano, Y. Kitagawa, H. T. Karimata, N. Sugimoto
  • Journal Title: Nucleic Acids Symp. Ser. 52 Pages: 519-520
• [Journal Article] Thermodynamics of DNA structures under molecular crowding conditions with neutral and positive charged cosolutes (2008)
  • Author(s): D. Miyoshi, K. Nakamura, S. Muhuli, H. T. Karimata, N. Sugimoto
  • Journal Title: Nucleic Acids Symp. Ser. 52 Pages: 413-414
• [Journal Article] Enhanced amplification of polymerase chain reaction by addition of cosolutes derived from a cellular compatible solute (2008)
  • Author(s): K. Koumoto, H. Ochiai, N. Sugimoto
  • Journal Title: Nucleic Acids Symp. Ser. 52 Pages: 257-258
• [Journal Article] Hydration is an important factor to regulate thermodynamic stability, of a DNA duplex under molecular crowding condtions (2008)
  • Author(s): K. Koumoto, H. Ochiai, N. Sugimoto
  • Journal Title: Chem. Lett. 37 Pages: 864-865
  • Peer Reviewed
• [Journal Article] DNAはなぜ二重らせん構造になるのか? -分子間相互作用と分子環境効果を考える- (2008)
  • Author(s): 杉本直己・中野修一
  • Journal Title: 現代化学 450 Pages: 25-30
• [Journal Article] 麹菌をタンパク質工場にする化学-いつでも好きなだけタンパク質をつくれるスイッチRNA- (2008)
  • Author(s): 山内隆寛・杉本直己
  • Journal Title: 化学 63 Pages: 36-40
• [Journal Article] 外部刺激に応答する機能性核酸-核酸ならではの機能を活用したナノバイオマテリアルの設計 (2008)
  • Author(s): 三好大輔・杉本直己
  • Journal Title: 化学工業 59 Pages: 21-27
• [Journal Article] Novel catalytic activity of nitrile hydratase from Rhodococcus sp. N771 (2008)
  • Author(s): Taniguchi, K., Murata, K., Murakami, J., Takahashi, S., Nakamura, T., Hashimoto, K., Koshino, H., Dohmae, N., Yohda, M., Hirose, T., Maeda, M. Odaka, M.
  • Journal Title: J Biosci Bioeng 106 Pages: 174-179
  • Peer Reviewed
• [Journal Article] Formation of highly toxic soluble amyloid beta oligomers by the molecular chaperone prefoldin (2008)
  • Author(s): Sakono, M., Zako, T., Ueda, H., Yohda, M. Maeda, M.
  • Journal Title: FEBS J 275 Pages: 5982-5993
  • Peer Reviewed
• [Journal Article] Role of the IXI/V motif in oligomer assembly and function of StHsp14.0, a small heat shock protein from the acidothermophilic archaeon, Sulfolobus tokodaii strain 7 (2008)
  • Author(s): Saji, H., Iizuka, R., Yoshida, T., Abe, T., Kidokoro, S., Ishii, N. Hohda, M.
  • Journal Title: Proteins 71 Pages: 771-782
  • Peer Reviewed
• [Journal Article] Overexpression of prefoldin from the hyperthermophilic archaeum Pyrococcus horikoshii OT3 endowed Escherichia coli with organic soluent tolerance (2008)
  • Author(s): Okochi, M., Kanie, K., Kurimoto, M., Yohda, M. Honda, H.
  • Journal Title: Appl Microbiol Biotechnol 79 Pages: 443-449
  • Peer Reviewed
• [Journal Article] Structure of aspartate racemase complexed with a dual substrate analogue, citric acid, and implications for the reaction mechanism (2008)
  • Author(s): Ohtaki, A., Nakano, Y., Iizuka, R., Arakawa, T., Yamada, K., Odaka, M. Yohda, M.
  • Journal Title: Proteins 70 Pages: 1167-1174
  • Peer Reviewed
• [Journal Article] Modulation of redox potential and alteration in reactivity via the peroxide shunt pathway by mutation of cytochrome P450 around the proximal heme ligand (2008)
  • Author(s): Matsumura, H., Wakatabi, M., Omi, S., Ohtaki, A., Nakamura, N., Yohda, M. Ohno, H
  • Journal Title: Biochemistry 47 Pages: 4834-4842
  • Peer Reviewed
• [Journal Article] Dynamics of group II chaperonin and prefoldin probed by 13C NMR spectroscopy (2008)
  • Author(s): Kurimoto, E., Nishi, Y., Yamaguchi, Y., Zako, T., Iizuka, R., Ide, Yohda, M. Kato, K.
  • Journal Title: Proteins 70 Pages: 1257-1263
  • Peer Reviewed
• [Journal Article] Structural and molecular characterization of the prefoldin beta subunit from Thermococcus strain KS-1 (2008)
  • Author(s): Kida, H., Sugano, Y., Iizuka, R., Fujihashi, M., Yohda, M. Miki, K.
  • Journal Title: J Mol Biol 383 Pages: 465-474
  • Peer Reviewed
• [Journal Article] Sequential action of ATP-dependent subunit conformational change and interaction between helical protrusions in the closure of the built-in lid of group II chaperonins (2008)
  • Author(s): Kanzaki, T., Iizuka, R., Takahashi, K., Maki, K., Masuda, R., Sahlan, M., Yebenes, H., Valpuesta, J. M., Oka, T., Furutani, M., Ishii, N., Kuwajima, K. Yohda, M.
  • Journal Title: J Biol Chem 283 Pages: 34773-34784
  • Peer Reviewed
• [Journal Article] Crystal structure of an extensively simplified variant of bovine pancreatic trypsin inhibitor in which over one-third of the residues are alanines (2008)
  • Author(s): Islam, M. M., Sohya, S., Noguchi, K., Yohda, M. Kuroda, Y.
  • Journal Title: Proc Natl Acad Sci USA 105 Pages: 15334-15339
  • Peer Reviewed
• [Journal Article] Functional characterization of recombinant prefoldin complexes from a hyperthermophilic archaeon, Thermococcus sp. strain KS-1 (2008)
  • Author(s): Iizuka, R., Sugano, Y., Ide, N., Ohtaki, A., Yoshida, T., Fujiwara, S., Imanaka, T. Yohda, M.
  • Journal Title: J Mol Biol 377 Pages: 972-983
  • Peer Reviewed
• [Journal Article] Catalytic mechanism of nitrile hydratase proposed by time-resolved X-ray crystallography using a novel substrate, tart-butysionitrile (2008)
  • Author(s): Hashimoto, K., Suzuki, H., Taniguchi, K., Noguchi, T., Yohda, M. Odaka, M.
  • Journal Title: J Biol Chem 283 Pages: 36617-36623
  • Peer Reviewed
• [Journal Article] Toward Autonomously Operating Molecular Machines Driven by Transition-Metal Catalyst (2008)
  • Author(s): Kenichi Tanaka and Kazushi Kinbara
  • Journal Title: Molecular BioSystems 4 Pages: 512-514
  • Peer Reviewed
• [Journal Article] Toward Long-Distance Mechanical Communication : Studies on a Ternary Complex Interconnected by a Bridging Rotary Module (2008)
  • Author(s): Hiroyuki Kai, Shinji Nara, Kazushi Kinbara, and Takuzo Aida
  • Journal Title: Journal of the Amerincan Chemical Society 130 Pages: 6725-6727
  • Peer Reviewed
• [Presentation] NanoBioNow(17)麹菌由来リボスイッチの機能改変 (2009)
  • Author(s): 山内隆寛、三好大輔、徳井美里、神谷久弥、松永將義、杉本直己
  • Organizer: 日本化学会第89春季年会
  • Place of Presentation: 千葉
  • Year and Date: 2009-03-30
• [Presentation] NanoBioNow(18)DNAのリン酸負電荷とカチオンのクーロン相互作用に対する定量的アプローチ (2009)
  • Author(s): 北川雄一、中野修一、杉本直己
  • Organizer: 日本化学会第89春季年会
  • Place of Presentation: 千葉
  • Year and Date: 2009-03-30
• [Presentation] NanoBioNow(15)安定なペンタループ形成の熱力学的パラメータの算出 (2009)
  • Author(s): 田中裕子、岸本加恵、川上純司、杉本直己
  • Organizer: 日本化学会第89春季年会
  • Place of Presentation: 千葉
  • Year and Date: 2009-03-29
• [Presentation] NanoBioNow(16)ヘアピンRNAの二次構造変化に及ぼすカチオンの静電相互作用の影響 (2009)
  • Author(s): 平山英伸、中野修一、杉本直己
  • Organizer: 日本化学会第89春季年会
  • Place of Presentation: 千葉
  • Year and Date: 2009-03-29
• [Presentation] NanoBioNow(19)テロメアDNAの構造安定性とテロメラーゼ活性の相関 (2009)
  • Author(s): 三村健太、Hai-Quing Yu、Dong-Hao Zhang、三好大輔、杉本直己
  • Organizer: 日本化学会第89春季年会
  • Place of Presentation: 千葉
  • Year and Date: 2009-03-29
• [Presentation] NanoBioNow(20)DNA四重鎖構造の熱力学的安定性に及ぼすループ領域の役割 (2009)
  • Author(s): 藤本健史、三好大輔、杉本直己
  • Organizer: 日本化学会第89春季年会
  • Place of Presentation: 千葉
  • Year and Date: 2009-03-29
• [Presentation] NanoBioNow(21)DNAの構造安定性に及ぼすヒストン模倣ペプチドの効果 (2009)
  • Author(s): 三好大輔、中村かおり、Muhuri Sanjukta、杉本直己
  • Organizer: 日本化学会第89春季年会
  • Place of Presentation: 千葉
  • Year and Date: 2009-03-29
• [Presentation] NanoBioNow(23)紫外線照射サケ白子DNAの紫外線吸収スペクトル (2009)
  • Author(s): 佐々木義晴、三好大輔、杉本直己
  • Organizer: 日本化学会第89春季年会
  • Place of Presentation: 千葉
  • Year and Date: 2009-03-29
• [Presentation] Nucleic acid interaction revisited : Cation and water binding to nucleotides (2009)
  • Author(s): 中野修一
  • Organizer: 日本化学会第89春季年会
  • Place of Presentation: 千葉
  • Year and Date: 2009-03-29
• [Presentation] モレキュラーグルー(分子糊)を用いたタンパク質の超分子的修飾 (2009)
  • Author(s): 大黒耕ら
  • Organizer: 日本化学会第89春季年会
  • Place of Presentation: 日本大学理工学部船橋キャンパス
  • Year and Date: 2009-03-29
• [Presentation] キラルなアミノ酸、4-アミノピペリジンー4-カルボン酸、からなる新規なオリゴペプチドの分子設計と性質 (2009)
  • Author(s): 田中雅洋ら
  • Organizer: 日本化学会第89春季年会
  • Place of Presentation: 日本大学理工学部船橋キャンパス
  • Year and Date: 2009-03-29
• [Presentation] NanoBioNow(22)適合溶質の酵素活性に及ぼす影響 (2009)
  • Author(s): 出口瑛介、甲元一也、杉本直己
  • Organizer: 日本化学会第89春季年会
  • Place of Presentation: 千葉
  • Year and Date: 2009-03-27
• [Presentation] 核酸の高次構造を活かした新機能性材料の創製 (2009)
  • Author(s): 杉本直己
  • Organizer: 有機材料研究会九州支部
  • Place of Presentation: 北九州市
  • Year and Date: 2009-02-28
• [Presentation] 体シグナル解析用分子として活用できる機能性核酸センサーの定量的デザイン (2009)
  • Author(s): 杉本直己
  • Organizer: 第7回ライフサーベイヤシンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2009-01-10
• [Presentation] Self-assembled semi-wet materials for nano-biotechnology (2008)
  • Author(s): Itaru Hamachi
  • Organizer: 2008 Bionano-sympo in Tokyo Tech
  • Place of Presentation: Yokohama
  • Year and Date: 20080508-10
• [Presentation] フォトクロミック配位子を有する遷移金属錯体触媒の設計と重合反応の可変立体制御 (2008)
  • Author(s): 宮村泰直、金原数、相田卓三
  • Organizer: 第57回高分子討論会
  • Place of Presentation: 大阪府立大学
  • Year and Date: 2008-09-25
• [Presentation] シャペロニンナノチューブの構築と拡張 (2008)
  • Author(s): ビスワス シュヴェンドゥら
  • Organizer: 第57回高分子討論会
  • Place of Presentation: 大阪府立大学
  • Year and Date: 2008-09-25
• [Presentation] アキラルなアミノ酸「4-アミノピペリジンー4-カルボン酸」からなる新規なオリゴペプチドの設計 (2008)
  • Author(s): 趙〓一、金原数、相田卓三
  • Organizer: 第57回高分子討論会
  • Place of Presentation: 大阪府立大学
  • Year and Date: 2008-09-25
• [Presentation] タンパク質の超分子的な修飾を可能にするモレキュラーグルーの開拓と性質 (2008)
  • Author(s): 大黒耕、金原数、津本浩平、相田卓三
  • Organizer: 第57回高分子討論会
  • Place of Presentation: 大阪府立大学
  • Year and Date: 2008-09-25
• [Presentation] Artificial DNA forming metal-responding nanowire (2008)
  • Author(s): D. Miyoshi, H. T. Karimata, Z. -M. Wang, K. Koumoto, N. Sugimoto
  • Organizer: RSC Conference ; Chemistry in the New World of Bioengineering and Synthetic Biology
  • Place of Presentation: Oxford(イギリス)
  • Year and Date: 2008-09-22
• [Presentation] 分子クラウディング環境によってもたらされる核酸とカチオンの結合性の向上とその生物学的意義 (2008)
  • Author(s): 中野修一・桐畑俊正・平山英伸・杉本直己
  • Organizer: 第3回バイオ関連化学合同シンポジウム
  • Place of Presentation: 横浜
  • Year and Date: 2008-09-18
• [Presentation] ハンマーヘッドリボザイムの切断活性変化を利用したカチオンと共存核酸の結合の評価 (2008)
  • Author(s): 北川雄一・中野修一・杉本直己
  • Organizer: 第3回バイオ関連化学合同シンポジウム
  • Place of Presentation: 横浜
  • Year and Date: 2008-09-18
• [Presentation] 分子クラウディング環境下におけるテロメラーゼ活性の検討 (2008)
  • Author(s): Zhang Dong-Hao・Yu Hai-Qing, 三好大輔, ・杉本直己
  • Organizer: 第3回バイオ関連化学合同シンポジウム
  • Place of Presentation: 横浜
  • Year and Date: 2008-09-18
• [Presentation] DNA四重鎖の構造形成におけるカチオンと水分子の役割 (2008)
  • Author(s): 藤本健史・三好大輔・三村健太・狩俣寿枝・杉本直己
  • Organizer: 第3回バイオ関連化学合同シンポジウム
  • Place of Presentation: 横浜
  • Year and Date: 2008-09-18
• [Presentation] ラショナル・ランダム融合設計によるATPセンサーペプチドの構築 (2008)
  • Author(s): 畠山智之・松井淳・玉置克之・杉本直己
  • Organizer: 第3回バイオ関連化学合同シンポジウム
  • Place of Presentation: 横浜
  • Year and Date: 2008-09-18
• [Presentation] Trlple Helix Bundle構造をとるペプチドジチオカルバメート鉄錯体においてペプチド長がBundle構造安定性に及ぼす効果 (2008)
  • Author(s): 山中敏志・西村宗十・藤井敏司・杉本直己
  • Organizer: 第3回バイオ関連化学合同シンポジウム
  • Place of Presentation: 横浜
  • Year and Date: 2008-09-18
• [Presentation] 核酸の構造安定性に及ぼす分子クラウディングとヒストン模倣ペプチドの影響 (2008)
  • Author(s): 三好大輔・中村かおり・Muhuri Sanjukta・狩俣寿枝・杉本直己
  • Organizer: 第3回バイオ関連化学合同シンポジウム
  • Place of Presentation: 横浜
  • Year and Date: 2008-09-18
• [Presentation] Molecular crowding effect on metal ion binding properties of the hammerhead ribozyme (2008)
  • Author(s): S. Nakano, Y. Kitagawa, H. T. Karimata, N. Sugimoto
  • Organizer: Joint Symposium of the 18th International Roundtable on Nucleosides, nucleotides and Nucleic Acids and the 35th International Symposium on Nucleic Acids Chemistry
  • Place of Presentation: 京都
  • Year and Date: 2008-09-11
• [Presentation] Enhanced amplification of polymerase chain reaction by addition of cosolutes derived from a cellular compatible solute (2008)
  • Author(s): K. Koumoto, H. Ochiai, N. Sugimoto
  • Organizer: Joint Symposium of the 18th International Roundtable on Nucleosides, nucleotides and Nucleic Acids and the 35th International Synposium on Nucleic Acids Chemistry
  • Place of Presentation: 京都
  • Year and Date: 2008-09-10
• [Presentation] Thermodynamics of DNA structures under molecular crowding conditions with neutral and positive charged cosolutes (2008)
  • Author(s): D. Miyoshi, K. Nakamura, S. Muhuli, H. T. Karimata, N. Sugimoto
  • Organizer: Joint Symposium of the 18th International Roundtable on Nucleosides, nucleotides and,Nucleic Acids and the 35th International Synposium on Nucleic Acids Chemistry
  • Place of Presentation: 京都
  • Year and Date: 2008-09-10
• [Presentation] Phosphorescent Mesostructured Silica Templated by an Amphiphilic Columnar Assembly of Trinuclear Au(I) Pyrazolate Complex (2008)
  • Author(s): Hendrik O. Lintang, et.al.
  • Organizer: IMMS 2008 6th International Mesostructured Materials Symposium
  • Place of Presentation: Namur, Belgium
  • Year and Date: 2008-09-09
• [Presentation] 生命科学を加速する分子ツールの開発 (2008)
  • Author(s): 浜地格
  • Organizer: 第4回バイオ機器研究会
  • Place of Presentation: 産業医科大(北九州)
  • Year and Date: 2008-08-08
• [Presentation] DNA nanostructures that respond to chemical stimuli (2008)
  • Author(s): 三好大輔・狩俣寿枝・Z. -M. Wang・甲元一也・杉本直己
  • Organizer: ICOMC2008
  • Place of Presentation: Rennes(フランス)
  • Year and Date: 2008-07-14
• [Presentation] 核酸の高次構造変化を利用した機能性ナノバイオマテリアルの開発 (2008)
  • Author(s): 三好大輔・Hai-Qing Yu・狩俣寿枝・杉本直己
  • Organizer: ナノバイオ若手ネットワーキングシンポジウム
  • Place of Presentation: 静岡
  • Year and Date: 2008-06-13
• [Presentation] Designed peptide chips for protein analyses (2008)
  • Author(s): Hisakazu Mihara
  • Organizer: Korean Biochio Conference
  • Place of Presentation: Daejon, Korea
  • Year and Date: 2008-06-12
• [Presentation] スマートバイオマテリアルとしての超分子ヒドロゲル (2008)
  • Author(s): 浜地格
  • Organizer: 08-2ポリマーフロンティア
  • Place of Presentation: 東京工業大学
  • Year and Date: 2008-06-05
• [Presentation] DNA and RNA structures in the presence of a neutral cosolute (2008)
  • Author(s): 中野修一・Lei Wu・岡裕人・狩俣寿枝・桐畑俊正・佐藤雄一・藤井敏司・酒井宏・桑原正靖・澤井宏明・杉本直己
  • Organizer: Gordon Research Conference (Nucleic Acids)
  • Place of Presentation: Newport(アメリカ)
  • Year and Date: 2008-06-01
• [Presentation] シャペロニンをユニットとした刺激応答性超分子構造体の構築 (2008)
  • Author(s): ビスワス シュヴェンドゥら
  • Organizer: 第57回高分子学会年次大会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2008-05-29
• [Presentation] モレキュラーグルー(分子糊)を用いたタンパク質修飾 (2008)
  • Author(s): 大黒耕・金原数・津本浩平・相田卓三
  • Organizer: 第57回高分子学会年次大会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2008-05-28
• [Presentation] Phosphorescent Mesostructured Silica Templated by a Columnar Assembly of Trinuclear, Au(I) Pyrazolate Complex Formed via a Metallophilic Interaction (2008)
  • Author(s): Hendrik O. Lintang, et.al.
  • Organizer: 第57回高分子学会年次大会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2008-05-28
• [Presentation] 分子クラウディング状態における長鎖テロメアDNAの構造と安定性 (2008)
  • Author(s): 三好大輔・Hai-Qing Yu・Dong-Hao Zhang・杉本直己
  • Organizer: 日本ケミカルバイオロジー研究会第3回年会
  • Place of Presentation: 東京
  • Year and Date: 2008-05-20
• [Presentation] Development of Functional Molecules Based on Nucleic Acids (2008)
  • Author(s): 三好大輔・中野修一・狩俣寿枝・井上真美子・中村かおり・岡裕人・Zhong-Ming Wnag・甲元一也・杉本直己
  • Organizer: 2008 Medical Chemistry and Developmental Therapeutics Symposium
  • Place of Presentation: Tucson(アメリカ)
  • Year and Date: 2008-04-17
• [Presentation] DNAからDBへ-核酸(DNA)を活用するデザイナブルナノバイオ(DNB)エンジニアリング- (2008)
  • Author(s): 杉本直己
  • Organizer: 第26回医用高分子研究会講座
  • Place of Presentation: 東京
  • Year and Date: 2008-01-11
• [Book] バイオ研究のフロンティア2、酵素・タンパク質をはかる・とらえる・利用する (2009)
  • Author(s): 岡畑恵雄・三原久和
  • Total Pages: 188
  • Publisher: 工学図書
• [Book] 超分子サイエンス〜基礎から材料への展開〜 (2009)
  • Author(s): 杉本直己、三好大輔
  • Publisher: 機能性核酸のテーラーメード設計
• [Book] 核酸医薬の最前線 (2009)
  • Author(s): 三好大輔、杉本直己
  • Total Pages: 1-11
  • Publisher: 核酸医薬の分子設計
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