2009 Fiscal Year Final Research Report
Real-World Modeling of Self-Emergent Active Cell
| Project/Area Number |
17076007
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| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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| Allocation Type | Single-year Grants |
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| Review Section |
Science and Engineering
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| Research Institution | Kyoto University |
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Principal Investigator |
吉川 研一 Kyoto University, 大学院・理学研究科, 教授 (80110823)
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| Co-Investigator(Kenkyū-buntansha) |
SETO Hideki 京都大学, 大学院・理学研究科, 准教授 (60216546)
KITATAHA Hiroyuki 京都大学, 大学院・理学研究科, 助教 (20378532)
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| Project Period (FY) |
2005 – 2009
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| Keywords | リポソーム / 人工細胞モデル / その場観察 / DNA折り畳み転移 / 自己組織化 / ナノ構造制御 / 油中水滴 / レーザーピンセット |
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| Research Abstract |
The main purpose of this project is the construction of "non-equilibrium dissipative"artificial cell models. To achieve the purpose, the following three axes are employed; (i) in vitro re-construction of chromosome, (ii) transcriptional regulation by DNA coil-globule transition, (iii) cell-sized space specificity in the dynamics of membrane, DNA, and protein. The representative results in relation to the cell engineering are as follows; (i) Establishment of the methodology to entrap bio-polymers and substrates in a cell-sized model system. (ii) Unraveling of the scenario on the higher-order structural transition of chromatin. (iii) Invention of the technique of noninvasive and noncontact manipulation of bio-macromolecules by laser. Main results on basic science are; (i) Clear experimental representation on the confined effect of cell-sized space was demonstrated. For example, actin exhibits the two-step transition in a confined space, whereas it shows single step transduction in bulk phase. (ii) Clear evidence was shown on the cross-talk between membrane fluidity and the bulk viscosity.
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Research Products
(26 results)
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[Book] Methods in Enzymology2009
Author(s)
K. Takiguchi, A. Yamada, M. Negishi, M. Honda, Y. Tanaka-Takiguchi, K. Yoshikawa
Total Pages
31-53,22
Publisher
Elsevier Inc.
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