2009 Fiscal Year Final Research Report
Development of a genome-scale in vitro transcription and translation-coupled system
| Project/Area Number |
17076009
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| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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| Allocation Type | Single-year Grants |
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| Review Section |
Science and Engineering
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| Research Institution | Ritsumeikan University (2008-2009)
Kyoto University (2005-2007) |
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Principal Investigator |
IMANAKA Tadayuki Ritsumeikan University, 生命科学部, 教授 (30029219)
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| Co-Investigator(Kenkyū-buntansha) |
KANAI Tamotsu 京都大学, 工学研究科, 助教 (10346083)
YOSHIKAWA Yuko 環太平洋大学, 次世代教育学部, 教授 (80291871)
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| Project Period (FY) |
2005 – 2009
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| Keywords | 超好熱菌 / in vitro転写系 / in vitro翻訳系 / 再構成系 / ゲノム |
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| Research Abstract |
Transcription (RNA synthesis from DNA) and translation (Protein synthesis from RNA) are ubiquitous systems in all living organisms. An in vitro transcription system and an in vitro translation system were developed using stable cell components of a hyperthermophilic archaeon,Thermococcus kodakaraensis. A system for in vitro transcription and translation-coupled reactions was also constructed which enabled in vitro protein production from a DNA template. Furthermore, production of proteins from an operon, a cluster of genes transcribed as a single transcriptional unit, was also achieved.
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