Molecular mechanism of uptake and translocation of essential metal nutrients in plants.

2009 Fiscal Year Final Research Report

• Project/Area Number: 17078002
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: The University of Tokyo
• Principal Investigator: NISHIZAWA Naoko The University of Tokyo, 生物資源環境学部, 教授 (70156066)
• Co-Investigator(Kenkyū-buntansha): TAKAHASHI Michiko 宇都宮大学, 農学部, 准教授 (90345182)
• Project Period (FY): 2005 – 2009
• Keywords: ミネラル栄養 / 金属錯体トランスポーター / ニコチアナミン / ムギネ酸 / 環境耐性作物

Research Abstract

Iron is essential for most living organisms and is required for normal plant growth. We identified two novel cis-acting elements, IDE1 and IDE2, in the promoter of iron-deficiency-inducible IDS2 gene and two rice transcription factors, IDEF1 (IDE-binding factor 1) and IDEF2, which specifically bind to IDE1 and IDE2, respectively. IDEF1 efficiently binds to the CATGC sequence within IDE1. IDEF1 transcripts are constitutively present in rice roots and leaves. Transgenic rice plants expressing IDEF1 tolerate iron deficiency in calcareous soil. Expression analysis of these transgenic plants revealed that IDEF1 positively regulates iron-deficiency-induced genes, including the ferrous iron transporter gene OsIRT1 and the iron-deficiency-induced transcription factor gene OsIRO2. OsIRO2 regulates the expression of various Fe-deficiency-inducible genes including those for phytosiderophore biosynthesis. IDEF2 recognizes CA[A/C]G[T/C][T/C/A][T/C/A] within IDE2. IDEF2 transcripts are constitutively present in rice roots and leaves. Microarray analysis showed that IDEF2 is involved in the regulation of Fe-deficiency-inducible genes, including the Fe(II)-nicotianamine transporter gene OsYSL2. These data suggest the presence of a sequential gene regulatory network that functions via novel cis element/trans factor interactions to promote the iron-deficiency response.

Research Products

• [Journal Article] Phytosiderophore Efflux Transporters Are Crucial for Iron Acquisition in Graminaceous Plants. (2011)
  • Author(s): Nozoye T, Nagasaka S, Kobayashi T, Takahashi M, Sato Y, Sato Y, Uozumi N, Nakanishi H, Nishizawa NK.
  • Journal Title: The Journal of Biological Chemistry 286 Pages: 5446-5454
  • Peer Reviewed
• [Journal Article] The rice transcription factor IDEF1 is essential for the early response to iron deficiency and induces vegetative expression of late embryogenesis abundant genes. (2009)
  • Author(s): Kobayashi T, Nakanishi Itai R, Ogo Y, Kakei Y, Nakanishi H, Takahashi M, Nishizawa NK.
  • Journal Title: The Plant Journal 60 Pages: 948-961
  • Peer Reviewed
• [Journal Article] Iron fortification of rice seeds through activation of the nicotianamine synthase gene. (2009)
  • Author(s): Lee S, Jeon US, Lee SJ, Kim Y-K, Persson DP, Husted S, Schjorring JK, Kakei Y, Masuda H, Nishizawa NK, An G.
  • Journal Title: Proceedings of the National Academy of the USA 106 Pages: 22014-22019
  • Peer Reviewed
• [Journal Article] Formation of grain chalkiness and changes in water distribution in developing rice caryopses grown under high-temperature stress. (2009)
  • Author(s): Ishimaru T, K.Akemi K.Horigane, Ida M, Iwasawa N, Yumiko A.San-oh, Nakazono M, Nishizawa NK, Masumura T, Kondo M, Yoshida M.
  • Journal Title: Journal of Cereal Science 50 Pages: 166-174
  • Peer Reviewed
• [Journal Article] Rice OsYSL15 is an iron-regulated iron(III)-deoxymugineic acid transporter expressed in the roots and is essential for iron uptake in early growth of the seedlings. (2009)
  • Author(s): Inoue H, Kobayashi T, Nozoye N, Takahashi M, Kakei Y, Suzuki K, Nakazono M, Nakanishi H, Mori S, Nishizawa NK.
  • Journal Title: Journal of Biological Chemistry 284 Pages: 3470-3479
  • Peer Reviewed
• [Journal Article] Rice metal-nicotianamine transporter, OsYSL2, is required for the long-distance transport of iron and manganese. (2009)
  • Author(s): Ishimaru Y, Masuda H, Bashir K, Inoue H, Tsukamoto K, Takahashi M, Nakanishi H, Aoki N, Hirose T, Ohsugi R, Nishizawa NK.
  • Journal Title: The Plant Journal 62 Pages: 379-390
  • Peer Reviewed
• [Journal Article] Transgenic rice lines that include barley genes have increased tolerance to low iron availability in a calcareous paddy soil. (2008)
  • Author(s): Suzuki M, Morikawa CK, Nakanishi H, Takahashi M, Saigusa M, Mori S, Nishizawa NK.
  • Journal Title: Soil Science and Plant Nutrition 54 Pages: 77-85
  • Peer Reviewed
• [Journal Article] A Novel NAC Transcription Factor, IDEF2, That Recognizes the Iron Deficiency-responsive Element 2 Regulates the Genes Involved in Iron Homeostasis in Plants. (2008)
  • Author(s): Ogo Y, Kobayashi T, Nakanishi Itai R, Nakanishi H, Kakei Y, Takahashi M, Toki S, Mori S, Nishizawa NK.
  • Journal Title: Journal of Biological Chemistry 283 Pages: 13407-13417
  • Peer Reviewed
• [Journal Article] Mutational reconstructed ferric chelate reductase confers enhanced tolerance in rice to iron deficiency in calcareous soil. (2007)
  • Author(s): Ishimaru Y, Kim S, Tsukamoto T, Oki H, Kobayashi T, Watanabe S, Matsuhashi S, Takahashi M, Nakanishi H, Mori S, Nishizawa NK.
  • Journal Title: Proceedings of the National academy of Science of the USA 104 Pages: 7373-7378
  • Peer Reviewed
• [Journal Article] The transcription factor IDEF1 regulates the response to and tolerance of iron deficiency in plants. (2007)
  • Author(s): Kobayashi T, Ogo Y, Nakanishi Itai R, Nakanishi H, Takahashi M, Mori S, Nishizawa NK.
  • Journal Title: Proceedings of the National Academy of Science of the USA 104 Pages: 19150-19155
  • Peer Reviewed
• [Journal Article] The rice bHLH protein OsIRO2 is an essential regulator of the genes involved in Fe uptake under Fe-deficient conditions. (2007)
  • Author(s): Ogo Y, Nakanishi Itai R, Nakanishi H, Kobayashi T, Takahashi M, Mori S, Nishizawa NK
  • Journal Title: The Plant Journal 51 Pages: 366-377
  • Peer Reviewed
• [Journal Article] Rice plants take up iron as an Fe^<3+>-phytosiderophore and as Fe^<2+>. (2006)
  • Author(s): Ishimaru Y, Suzuki M, Tsukamoto T, Suzuki K, Nakazono M, Kobayashi T, Wada Y, Watanabe S, Matsuhashi S, Takahashi M, Nakanishi H, Mori S, Nishizawa NK.
  • Journal Title: The Plant Journal 45 Pages: 335-346
  • Peer Reviewed
• [Journal Article] Cloning and characterization of deoxymugineic acid synthase genes from graminaceous plants. (2006)
  • Author(s): Bashir K, Nagasaka S, Takahashi M, Nakanishi H, Mori S, Nishizawa NK.
  • Journal Title: Journal of Biological Chemistry 281 Pages: 32395-32402
  • Peer Reviewed
• [Journal Article] Biosynthesis and secretion of mugineic acid family phytosiderophores in zinc-deficient barley. (2006)
  • Author(s): Suzuki M, Takahashi M, Tsukamoto T, Watanabe S, Matsuhashi S, Yazaki J, Kishimoto N, Kikuchi S, Nakanishi H, Mori S, Nishizawa NK.
  • Journal Title: The Plant Journal 48 Pages: 85-97
  • Peer Reviewed
• [Journal Article] GAMYB controls different sets of genes and is differentially regulated by microRNA in aleurone cells and anthers. (2006)
  • Author(s): Tsuji H, Aya K, Ueguchi-Tanaka M, Shimada Y, Nakazono M, Watanabe R, Nishizawa NK, Gomi K, Shimada A, Kitano H, Ashikari M, Matsuoka M.
  • Journal Title: The Plant Journal 47 Pages: 427-444
  • Peer Reviewed
• [Journal Article] Expression of iron-acquisition-related genes in iron-deficient rice is co-ordinately induced by partially conserved iron-deficiency-responsive elements. (2005)
  • Author(s): Kobayashi T, Suzuki M, Inoue H, Nakanishi Itai R, Takahashi M, Nakanishi H, Mori S, Nishizawa NK.
  • Journal Title: Journal of Experimental Botany 56 Pages: 1305-1316
  • Peer Reviewed
• [Presentation] ワークショップ「チャネル・トランスポーターによる植物機能制御の分子基盤.Molecular basis of the regulation of plant growth and development by ion channels and transporters」 (2010)
  • Author(s): 西澤直子
  • Organizer: BMB2010(第33回日本分子生物学会年会・第83回日本生化学会大会合同大会)
  • Place of Presentation: 兵庫県・神戸市
  • Year and Date: 2010-12-10
• [Presentation] 平成22年度日本農学賞・読売農学賞受賞記念講演「植物の鉄栄養制御に関する研究」 (2010)
  • Author(s): 西澤直子
  • Organizer: 日本土壌肥料学会2010年度北海道大会
  • Place of Presentation: 北海道・札幌市
  • Year and Date: 2010-09-08
• [Presentation] The regulation of iron transport and homeostasis in Strategy II plants. (2010)
  • Author(s): Naoko Nishizawa
  • Organizer: the 15th International Symposium on Iron Nutrition and Interactions in Plants
  • Place of Presentation: ハンガリー・ブダペスト
  • Year and Date: 2010-07-27
• [Presentation] 植物によるカドミウムのレメディエーション (2010)
  • Author(s): 西澤直子
  • Organizer: 日本学術会議公開シンポジウム「植物を活かす-植物を利用したグリーンイノベーションに向けて」
  • Place of Presentation: 東京
  • Year and Date: 2010-05-29
• [Presentation] 鉄栄養制御の分子機構と新機能作物作出への応用 (2010)
  • Author(s): 西澤直子
  • Organizer: 中部土壌肥料研究会春の講演会
  • Place of Presentation: 愛知県・名古屋市
  • Year and Date: 2010-05-26
• [Presentation] 植物の鉄栄養とその制御 (2010)
  • Author(s): 西澤直子
  • Organizer: 日本原子力研究開発機構生命科学研究シンポジウム2010
  • Place of Presentation: 東京
  • Year and Date: 2010-03-16
• [Presentation] Iron uptake and loading in the rice grain. (2009)
  • Author(s): Naoko Nishizawa
  • Organizer: The 6th International Rice Genetics Symposium(RG6)and 7^<th> International Symposium on Rice Fuctional Genomics
  • Place of Presentation: フィリピン・マニラ
  • Year and Date: 2009-11-16
• [Presentation] シンポジウム「植物の鉄欠乏応答の発現制御機構」検討課題「バイテク手法を用いた食料生産性向上を目指した実用的な作物開発研究の方向」 (2009)
  • Author(s): 西澤直子
  • Organizer: 平成20年度北海道農業試験研究推進会議生物工学部会
  • Place of Presentation: 北海道・札幌市
  • Year and Date: 2009-02-02
• [Presentation] The novel transcription factors IDEF1 and IDEF2 regulate the iron deficiency response and tolerance in rice through binding to rion deficiency-responsive elements. (2008)
  • Author(s): Naoko K.Nishizawa
  • Organizer: The 6^<th> International Symposium of Rice Fuctional Genomics
  • Place of Presentation: 韓国・済州島
  • Year and Date: 20081110-20081112
• [Presentation] 農耕地の拡大を目指した不良土壌耐性作物の作出 (2008)
  • Author(s): 西澤直子
  • Organizer: 第10回RIBSバイオサイエンスシンポジウム「食糧・バイオマス生産性の飛躍的向上に向けて」
  • Place of Presentation: 岡山県・岡山市
  • Year and Date: 2008-10-31
• [Presentation] 極限土壌における植物の耐性戦略に関わる分子とその応用 (2008)
  • Author(s): 西澤直子
  • Organizer: 極限環境微生物学会第9回シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2008-07-11
• [Presentation] Molecules required for the iron deficiency response in plants. (2008)
  • Author(s): Naoko Nishizawa
  • Organizer: FASEB Summer Research Conferences
  • Place of Presentation: アメリカ・コロラド
  • Year and Date: 2008-06-18
• [Presentation] OsYSL family transporters involved in the uptake and translocation of iron in rice plants. (2006)
  • Author(s): Naoko K.Nishizawa
  • Organizer: ISINIP2006
  • Place of Presentation: フランス・モンペリエ
  • Year and Date: 2006-07-04
• [Presentation] Mineral Nutrients and Organelle. (2006)
  • Author(s): Naoko K.Nishizawa
  • Organizer: The 53rd NIBB Conference "Dynamic Organelles in Plants"特定領域研究「植物の環境適応戦略としてのオルガネラ分化」
  • Place of Presentation: 愛知県・岡崎市
  • Year and Date: 2006-06-17
• [Presentation] 不良土壌耐性遺伝子をヒトの健康に役立てる (2006)
  • Author(s): 西澤直子
  • Organizer: イネゲノム解読記念シンポジウム
  • Place of Presentation: 茨城県・つくば市
  • Year and Date: 2006-03-22
• [Presentation] Rice metal-nicotianamine and iron-phytosiderophore transporters involved in the uptake, translocation and accumulation of minerals in grains. (2005)
  • Author(s): Naoko K.Nishizawa
  • Organizer: 5th International Rice Genetics Symposium
  • Place of Presentation: フィリピン・マニラ
  • Year and Date: 20051119-20051123
• [Presentation] 植物の養分吸収と循環系明日を担う植物科学 (2005)
  • Author(s): 西澤直子
  • Organizer: シンポジウム-植物科学:分子動態から群落生産まで-
  • Place of Presentation: 東京
  • Year and Date: 2005-10-14
• [Presentation] Rice metal-nicotianamine and iron-phytosiderophore transporters involved in the uptake and translocation of metal nutrients. (2005)
  • Author(s): Nishizawa NK
  • Organizer: XV IPNC 2005
  • Place of Presentation: 中国・北京
  • Year and Date: 2005-09-15
• [Presentation] 植物細胞における小胞体由来構造の解析 (2005)
  • Author(s): 西澤直子
  • Organizer: 日本顕微鏡学会第61回学術講演会
  • Place of Presentation: 茨城県・つくば市
  • Year and Date: 2005-06-01
• [Book] Recent insights into iron homeostasis and their application in graminaceous crops.Proceedings of the Japan Academy, Series B(Proc.Jpn.Acad., Ser.B)Vol.86, No.9 (2010)
  • Author(s): Kobayashi T, Nakanishi H, Nishizawa NK.
  • Total Pages: 900-913
• [Book] Regulation of Iron and Zinc Uptake and Translocation in Rice.Rice Biology in the Genomics Era Biotechnology in Agriculture and Forestry 62(eds by H.-Y.Hirano et al.) (2008)
  • Author(s): Kobayashi T, Nishizawa NK.
  • Total Pages: 321-335
  • Publisher: Springer
• [Book] Deoxymugineic Acid Synthase.A Gene Important for Fe-Acquisition and Homeostasis.Plant Signaling & Behavior vol.1 Issue 6 (2006)
  • Author(s): Bashir K, Nishizawa NK.
  • Total Pages: 290-292
  • Publisher: Landes Bioscience
• [Book] olecular analysis of iron-deficient Graminaceous plants.In Iron Nutrition in Plants and Rhizospheric Microorganisms (eds by L.L.Barton and J.Abadia) (2006)
  • Author(s): Kobayashi T, Nishizawa NK, Mori S.
  • Total Pages: 395-435
  • Publisher: Springer Inc.
• [Remarks] ホームページ等
  • URL: http://park.itc.u-tokyo.ac.jp/syokuei/transport/index.html
• [Patent(Industrial Property Rights)] 植物の鉄欠乏耐性を向上させるポリペプチドおよびその利用 (2008)
  • Inventor(s): 独立法人科学技術振興機構
  • Industrial Property Rights Holder: 西澤直子、森敏、小林高範、小郷裕子
  • Industrial Property Number: 特許,特願2009-512948
  • Filing Date: 2008-04-24
• [Patent(Industrial Property Rights)] デオキシムギネ酸合成酵素およびその利用 (2006)
  • Inventor(s): 独立法人科学技術振興機構
  • Industrial Property Rights Holder: 西澤直子、森敏
  • Industrial Property Number: 特許,特願2006-218548
  • Filing Date: 2006-08-10