Study on plant Na and K transport system, which is involved in the formation of membrane potential and adaptation of osmolality

2009 Fiscal Year Final Research Report

• Project/Area Number: 17078005
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Tohoku University (2006-2009); Nagoya University (2005)
• Principal Investigator: UOZUMI Nobuyuki Tohoku University, 大学院・工学研究科, 教授 (40223515)
• Project Period (FY): 2005 – 2009
• Keywords: 浸透圧 / イオンチャネル / 塩 / ナトリウム / カリウム / 膜輸送 / 膜電位

Research Abstract

The membrane transport system is closely involved in the formation of membrane potential and adaptation to the abiotic stress. We aimed the physiological role of the channels and the transporters which mediated Na and/or K permeation across the plasma membrane and thylakoid membrane. There are some difference in Na and K transport system between animal cells and plant cells. We have studied the structure and function of the Na/K transporters and K channels in plant cells and cyanobacteria which are an essential system for the controlling the solute flux across the membrane.

Research Products

• [Journal Article] Phytosiderophore efflux transporters are crucial for iron acquisition in graminaceous plants. (2011)
  • Author(s): Nozoye, T., Nagasaka, S., Kobayashi, T., Takahashi, M., Sato, Y., Sato, Y., Uozumi, N., Nakanishi, H., Nishizawa, NK.
  • Journal Title: J.Biol.Chem. 286 Pages: 5446-5454
  • Peer Reviewed
• [Journal Article] AtKUP/HAK/KT9, a K^+ transporter from Arabidopsis thaliana, mediates Cs^+ uptake in Escherichia coli. (2010)
  • Author(s): Kobayashi, D., Uozumi, N., Hisamatsu, S., Yamagami, M.
  • Journal Title: Biosci.Biotech.Biochem. 74 Pages: 203-205
  • Peer Reviewed
• [Journal Article] Modulation of the Arabidopsis KAT1 channel by an activator of protein kinase C in Xenopus laevis oocytes. (2010)
  • Author(s): Sato, A., Gambale, F., Dreyer, I., Uozumi, N.
  • Journal Title: FEBS J. 277 Pages: 2318-2328
  • Peer Reviewed
• [Journal Article] Trk/HKT-type K^+ transporter from Trypanosoma brucei. (2010)
  • Author(s): Mosimann, M., Goshima, S., Wenzler, Y., Luscher, A., Uozumi, N., Maser, P.
  • Journal Title: Eukaryotic Cell 9 Pages: 539-546
  • Peer Reviewed
• [Journal Article] A novel potassium channel in photosynthetic cyanobacteria. (2010)
  • Author(s): Zanetti, M., Teardo, E., Rocca, N. L., Zulkifli, L., Checchetto, V., Shijuku, T, Sato, Y., Giacometti, G. M., Uozumi, N., Bergantino, E., Szabo, I.
  • Journal Title: PLoS ONE 5 Pages: e10118,1-10
  • Peer Reviewed
• [Journal Article] Roles of tandem-pore K^+ channels in plants-a puzzle still to be solved. (2010)
  • Author(s): Voelker, C., Gomez-Porras, J. L., Becker, D., Hamamoto, S., Uozumi, N., Gambale, F., Mueller-Roeber, B., Czempinski, K., Dreyer, I.
  • Journal Title: Plant Biology 12 Pages: 56-66
  • Peer Reviewed
• [Journal Article] Cell-free protein N-myristoylation system utilizing wheat-germ extracts facilitates update of consensus motif for the modification of plant proteins. (2010)
  • Author(s): Yamauchi, S., Fusada, N., Hayashi, H., Utsumi, T., Uozumi, N., Tozawa, Y.
  • Journal Title: FEBS.J. 277 Pages: 3596-3607
  • Peer Reviewed
• [Journal Article] The KtrA and KtrE subunits are required for Na^+ dependent K^+ uptake by KtrB across the plasma membrane in Synechocystis sp.PCC 6803. (2010)
  • Author(s): Zulkifli, L., Akai, M., Yoshikawa, A.,Shimojima, M., Ohta, H., Guy HR., Uozumi, N.
  • Journal Title: J.Bacteriol. 192 Pages: 5063-5070
  • Peer Reviewed
• [Journal Article] Contribution of salicylic acid glucosyltransferase, OsSGT1, to chemically-induced disease resistance in rice plants. (2009)
  • Author(s): Umemura, K., Satoh, J., Iwata, M., Uozumi, N., Koga, J., Kawano, T., Koshiba, T., Anzai, H., Mitomi, M.
  • Journal Title: Plant J. 57 Pages: 463-472
  • Peer Reviewed
• [Journal Article] Identification and characterization of the Na^+/H^+ antiporter NhaS3 from the thylakoid membrane of Synechocystis sp.PCC 6803. (2009)
  • Author(s): Tsunekawa, K., Shijuku, T., Hayashimoto, M., Kojima, T., Onai, K., Morishita, M., Ishiura, M., Kuroda, T., Nakamura, T., Kobayashi, H., Sato, M., Toyooka, K., Matsuoka, K., Omata, T., Uozumi, N.
  • Journal Title: J.Biol.Chem. 284 Pages: 16513-16521
  • Peer Reviewed
• [Journal Article] Synchrony between flower opening and petal-color change from red to blue in morning glory, Ipomoea tricolor cv. Heavenly Blue. (2009)
  • Author(s): Yoshida, K., Miki, N., Momonoi, K., Kawachi, M., Katou, K., Okazaki, Y., Uozumi, N., Maeshima, M., Kondo, T.
  • Journal Title: Proc.Jpn.Acad.Ser.B 85 Pages: 187-197
  • Peer Reviewed
• [Journal Article] Threonine at position 306 of the KAT1 potassium channel is essential for channel activity and is a target site for ABA-activated SnRK2/OST1/SnRK2.6 protein kinase. (2009)
  • Author(s): Sato, A., Sato, Y., Fukao, Y., Fujiwara, M., Umezawa, T., Shinozaki, K., Hibi, T., Taniguchi, M., Miyake, H., Goto, D.B., Uozumi, N.
  • Journal Title: Biochem.J. 424 Pages: 439-448
  • Peer Reviewed
• [Journal Article] The implication of YggT of Escherichia coli in osmotic regulation. (2009)
  • Author(s): Ito, T., Uozumi, N., Nakamura, T., Takayama, S., Matsuda, N., Aiba, H., Hemmi, H., Yoshimura, T.
  • Journal Title: Biosci.Biotech.Biochem. 73 Pages: 2698-2704
  • Peer Reviewed
• [Journal Article] Characterization of a tobacco TPK-type K^+ channel as a novel tonoplast K^+ channel using yeast tonoplasts. (2008)
  • Author(s): Hamamoto, S., Marui, K., Matsuoka, K., Higashi, K., Igarashi, K., Nakagawa, T., Kuroda, T., Mori, Y., Murata, Y., Nakanishi, Y., Maeshima, M., Yabe, I., Uozumi, N.
  • Journal Title: J.Biol.Chem. 283 Pages: 1911-1920
  • Peer Reviewed
• [Journal Article] Purification of the functional plant membrane channel KAT1. (2008)
  • Author(s): Hibi, T., Aoki; S., Oda; K., Munemasa, S., Ozaki, S., Shirai, O., Uozumi, N.
  • Journal Title: Biochem.Biophys.Res.Commun. 374 Pages: 465-469
  • Peer Reviewed
• [Journal Article] Novel treatment for lithium induced nephrogenic diabetes insipidus rat model using the Sendai-virus vector carrying aquaporin 2 gene. (2008)
  • Author(s): Suga, H., Nagasaki, H., Kondo, T., Okajima, Y., Suzuki, C., Ozaki, N., Arima, H., Yamamoto, T., Ozaki, N., Akai, M., Sato, A., Uozumi, N., Inoue, M., Hasegawa, M., Oiso, Y.
  • Journal Title: Endocrinology 149 Pages: 5803-5810
  • Peer Reviewed
• [Journal Article] Electrophysiological property of NtTPK1 expressed in yeast tonoplast. (2008)
  • Author(s): Hamamoto, S., Yabe, I., Uozumi, N.
  • Journal Title: Biosci.Biotech.Biochem. 72 Pages: 2785-2787
  • Peer Reviewed
• [Journal Article] Contribution of hydrophobic and electrostatic interactions to the membrane integration of the Shaker K^+ channel voltage sensor domain. (2007)
  • Author(s): Zhang, L., Sato, Y., Hessa, T., von Heijne, G., Lee, J-K., Kodama, I., Sakaguchi, M., Uozumi, N.
  • Journal Title: Proc.Natl.Acad.Sci.USA 104 Pages: 8263-8268
  • Peer Reviewed
• [Journal Article] Role of positively charged amino acids in the M2_D transmembrane helix of Ktr/Trk/HKT-type cation transporters. (2007)
  • Author(s): Kato, N., Akai, M., Zulkifli, L., Matsuda, N., Kato, Y., Goshima, S., Hazama, A., Yamagami, M., Guy, H. R., Uozumi, N.
  • Journal Title: Channels 1 Pages: 161-171
  • Peer Reviewed
• [Journal Article] Ktr-mediated potassium transport, a major pathway for potassium uptake, is coupled to a proton gradient across the membrane in the Synechocystis sp.PCC6803. (2006)
  • Author(s): Matsuda, N., Uozumi, N.
  • Journal Title: Biosci.Biotech.Biochem. 70 Pages: 273-275
  • Peer Reviewed
• [Journal Article] Properties of Shaker-type potassium channels in higher plants. (2006)
  • Author(s): Gambale, F., Uozumi, N.
  • Journal Title: J.Membrane Biol. 210 Pages: 1-19
  • Peer Reviewed
• [Journal Article] Nomenclature for HKT transporters, key determinants of plant salinity tolerance. (2006)
  • Author(s): Platten, J.D., Cotsaftis, O., Berthomieu, P., Bohnert, H., Davenport, R.J., Fairbairn, D.J., Horie, T., Leigh, R.A., Lin, H-X., Luan, S., Maser, P., Pantoja, O., Rodriguez-Navarro, A., Schachtman, D.P., Schroeder, J.I., Sentenac, H., Uozumi, N., Very, A-A., Zhu, J-K., Dennis, E.S., Tester, M.
  • Journal Title: Trends in Plant Sciences 11 Pages: 372-374
  • Peer Reviewed
• [Journal Article] Mutation of His-157 in the second pore loop drastically reduces the activity of Synechocystis Ktr transporter. (2006)
  • Author(s): Zulkifli, L., Uozumi, N.
  • Journal Title: J.Bacteriol. 188 Pages: 7985-7987
  • Peer Reviewed
• [Journal Article] Further application of a two-step heparin affinity chromatography method using divalent cations as eluents : Purification and identification of membrane-bound heparinb inding proteins from the mitochondrial fraction of HL-60 cells. (2005)
  • Author(s): Iida, T., Masaharu, K., Uozumi, N., Inui, T., Imai, K.
  • Journal Title: J.Chromatography B 823 Pages: 209-212
  • Peer Reviewed
• [Journal Article] Enhanced salt tolerance mediated by AtHKT1 transporter-induced Na^+ unloading from xylem vessels to xylem parenchyma cells. (2005)
  • Author(s): Sunarpi, Horie, T., Motoda, Jo, Kubo, M., Yang, H., Yoda, K., Horie, R., Chan, W-Y., Leung, H-Y., Hattori, K., Konomi, M., Osumi, M., Yamagami, M., Schroeder, J.I.,U ozumi, N.
  • Journal Title: Plant J. 44 Pages: 928-938
  • Peer Reviewed
• [Journal Article] All four putative selectivity filter glycine residues in KtrB are essential for high affinity and selective K^+ uptake by the KtrAB system from Vibrio alginolyticus. (2005)
  • Author(s): Tholema, N., vor der Bruggen, M., Maser, P., Nakamura, T., Schroeder, J.I., Kobayashi, H., Uozumi, N., Bakker, E.P.
  • Journal Title: J.Biol.Chem. 280 Pages: 41146-41154
  • Peer Reviewed
• [Presentation] KAT1 is differentially phosphorylated by SnRK2.6 and calcium-dependent kinase, which reduces its transport activity. (2010)
  • Author(s): Uozumi N, Sato A, Sato Y, Fukao Y, Fujiwara M, Umezawa T, Shinozaki K, Hibi T, Taniguchi M, Miyake M, Dreyer I, Gambale F, Goto D.
  • Organizer: XV International Workshop on Plant Membrane Biology
  • Place of Presentation: Adelaide, Australia
  • Year and Date: 2010-09-23
• [Presentation] Phosphorylation Of KAT1 C-terminus Modulates K+ Uptake Activity (2009)
  • Author(s): Sato, A., Taniguchi, M., Miyake, H., Umezawa, T., Shinozaki, K., Goto, D.B., Uozumi, N.
  • Organizer: Biophysical Society 53rd Annual Meeting
  • Place of Presentation: Boston, USA
  • Year and Date: 2009-03-02
• [Presentation] Membrane topogenesis of the voltage dependent K channels (2007)
  • Author(s): Zhang, L., Uozumi, N.
  • Organizer: Membrane Transport as a Universal Biological Mechanism
  • Place of Presentation: Kyoto
  • Year and Date: 2007-01-11
• [Book] Ion Channels and Plant Stress Responses.(Ion channels and plant stress : past, present and future)(In Demidchik, V., Maathuis, F. (ed.)) (2010)
  • Author(s): Uozumi, N., Schroeder J.I.
  • Total Pages: 1-22
  • Publisher: Springer-Verlag Berlin Heidelberg.
• [Book] Comprehensive Biophysics Volume 6-Channel Proteins(Structure-Function Correlates in Plant Ion Channels.)(Montal, M.(ed.))
  • Author(s): Uozumi, N, Dreyer, I.
  • Publisher: Elsevier, in press
• [Remarks] ホームページ等