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2009 Fiscal Year Final Research Report

Isolation and characterization of Si transporter

Research Project

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Project/Area Number 17078008
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionOkayama University

Principal Investigator

MA Kenbou  Okayama University, 資源生物科学研究所, 教授 (80260389)

Co-Investigator(Kenkyū-buntansha) YAMAJI Naoki  岡山大学, 資源生物科学研究所, 助教 (00444646)
Project Period (FY) 2005 – 2009
Keywordsイネ / ケイ酸トランスポーター / 極性 / 吸収 / 分配
Research Abstract

Silicon (Si) is a beneficial element for plant growth, which helps plants to overcome multiple stresses. In this project, we have identified several transporters involved in Si uptake and distribution. Lsi1 and Lsi2 identified from rice are expressed in the roots and polarly localized at the exodermis and endodermis. These transporters are important for Si uptake by the roots. Lsi6 is localized at the xylem parenchyma of leaf and node I and responsible for distribution of Si. We also identified similar transporters from maize and pumpkin. We found that they have different expression pattern and localization from rice.

  • Research Products

    (79 results)

All 2009 2008 2007 2006 2005 2004 Other

All Journal Article (30 results) (of which Peer Reviewed: 25 results) Presentation (43 results) Book (2 results) Remarks (1 results) Patent(Industrial Property Rights) (3 results)

  • [Journal Article] A transporter at the node responsible for inter-vascular transfer of silicon in rice.2009

    • Author(s)
      Yamaji N, Ma JF.
    • Journal Title

      Plant Cell 21

      Pages: 2878-2883

    • Peer Reviewed
  • [Journal Article] The rice aquaporin Lsi1 mediates uptake of methylated arsenic species.2009

    • Author(s)
      Li RY, Ago Y, Liu W J, Mitani N, Feldmann J, McGrath SP, Ma JF, Zhao FJ.
    • Journal Title

      Plant Physiol. 150

      Pages: 2071-2080

    • Peer Reviewed
  • [Journal Article] Identification and characterization of maize and barley Lsi2-like silicon efflux transporters reveals a distinct silicon uptake system from that in rice.2009

    • Author(s)
      Mitani N, Chiba Y, Yamaji N, Ma JF.
    • Journal Title

      Plant Cell 21

      Pages: 2133-2142

    • Peer Reviewed
  • [Journal Article] Differential gene expression of rice in response to silicon and rice blast fungus Magnaporthe oryzae.2009

    • Author(s)
      Brunings AM, Datnoff LE, Ma JF, Mitani N, Nagamura Y, Rathinasabapathi B, Kirst M
    • Journal Title

      Ann. Appl. Biol. 155

      Pages: 161-170

    • Peer Reviewed
  • [Journal Article] Mitigation of arsenic accumulation in rice with water management and silicon fertilization.2009

    • Author(s)
      Li RY, Stroud JL, Ma JF, McGrath SP, Zhao FJ.
    • Journal Title

      Environ. Sci. Technol. 43

      Pages: 3778-3783

    • Peer Reviewed
  • [Journal Article] NIP1;1, an aquaporin homolog, determines the arsenite sensitivity of Arabidopsis thaliana.2009

    • Author(s)
      Kamiya T, Tanaka M, Mitani N, Ma JF, Maeshima M, Fujiwara T.
    • Journal Title

      J. Biol. Chem. 284

      Pages: 2114-2120

    • Peer Reviewed
  • [Journal Article] Effects of active silicon uptake by rice on 29Si fractionation in various plant parts.2009

    • Author(s)
      Koster JR, Bol R, Leng MJ, Parker AG, Sloane HJ, Ma JF.
    • Journal Title

      Rapid Commun. Mass Spectrom. 23

      Pages: 2398-2402

    • Peer Reviewed
  • [Journal Article] Arsenic uptake and metabolism in plants.2009

    • Author(s)
      Zhao FJ, Ma JF, Meharg AA, McGrath SP.
    • Journal Title

      New Phytol. 181

      Pages: 777-794

    • Peer Reviewed
  • [Journal Article] Defective active silicon uptake affects some components of rice resistance to brown spot.2009

    • Author(s)
      Dallagnol LJ, Rodrigues FA, Mielli MVB, Ma JF, Datnoff LE
    • Journal Title

      Phytopathology 99

      Pages: 116-121

    • Peer Reviewed
  • [Journal Article] HvLsi1 is a silicon influx transporter in barley.2009

    • Author(s)
      Chiba Y, Yamaji N, Mitani N, Ma JF.
    • Journal Title

      Plant J. 57

      Pages: 810-818

    • Peer Reviewed
  • [Journal Article] Identification of maize silicon influx transporters.2009

    • Author(s)
      Mitani N, Yamaji N, Ma JF.
    • Journal Title

      Plant Cell Physiol. 50

      Pages: 5-12

    • Peer Reviewed
  • [Journal Article] Transporters of arsenite in rice and their role in arsenic accumulation in rice grain.2008

    • Author(s)
      Ma JF, Yamaji N, Mitani N, Xu XY, Su YH, McGrath S, Zhao FJ.
    • Journal Title

      PNAS 105

      Pages: 9931-9935

    • Peer Reviewed
  • [Journal Article] Characterization of substrate specificity of a rice silicon transporter, Lsi1. Pflugers Arch-Eur.2008

    • Author(s)
      Mitani N, Yamaji N, Ma JF.
    • Journal Title

      J. Physiol. 456

      Pages: 679-686

    • Peer Reviewed
  • [Journal Article] Reexamination of silicon effects on rice growth and production under field conditions using a low silicon mutant.2008

    • Author(s)
      Tamai K, Ma JF.
    • Journal Title

      Plant Soil 307

      Pages: 21-27

    • Peer Reviewed
  • [Journal Article] A Transporter regulating silicon distribution in rice shoots.2008

    • Author(s)
      Yamaji N, Mitatni N, Ma JF.
    • Journal Title

      Plant Cell 20

      Pages: 1381-1389

    • Peer Reviewed
  • [Journal Article] Spatial distribution and temporal variation of the rice silicon transporter Lsi1.2007

    • Author(s)
      Yamaji N, Ma JF.
    • Journal Title

      Plant Physiol. 143

      Pages: 1306-1313

    • Peer Reviewed
  • [Journal Article] An efflux transporter of silicon in rice.2007

    • Author(s)
      Ma JF, Yamaji N, Mitani N, Tamai K, Konishi S, Fujiwara T, Katsuhara M, Yano M.
    • Journal Title

      Nature 448

      Pages: 209-211

    • Peer Reviewed
  • [Journal Article] イネのケイ素トランスポーター.2007

    • Author(s)
      馬建鋒、山地直樹、三谷奈見季
    • Journal Title

      蛋白質核酸酵素 52

      Pages: 1849-1856

  • [Journal Article] Genotypic difference in Si uptake and expression of Si transporter genes in rice.2007

    • Author(s)
      Ma JF, Yamaji N, Tamai K, Mitani N
    • Journal Title

      Plant Physiol. 145

      Pages: 919-924

    • Peer Reviewed
  • [Journal Article] イネにおけるケイ素の有益性及び吸収機構に関する研究.2007

    • Author(s)
      馬建鋒
    • Journal Title

      日本土壌肥料学雑誌 78

      Pages: 431-434

  • [Journal Article] A silicon transporter in rice.2006

    • Author(s)
      Ma JF, Tamai K, Yamaji N, Mitani N, Konishi S, Katsuhara M, Ishiguro M, Murata Y, Yano M.
    • Journal Title

      Nature 440

      Pages: 688-691

    • Peer Reviewed
  • [Journal Article] イネのケイ酸吸収機構.2006

    • Author(s)
      山地直樹、馬建鋒
    • Journal Title

      化学と生物 44

      Pages: 453-458

  • [Journal Article] イネのケイ酸輸送体.2006

    • Author(s)
      馬建鋒、山地直樹、三谷奈見季、玉井一規
    • Journal Title

      細胞工学 25

      Pages: 778-779

  • [Journal Article] Silicon uptake and accumulation in higher plants.2006

    • Author(s)
      Ma JF, Yamaji N
    • Journal Title

      Trends in Plant Sci. 11

      Pages: 392-397

    • Peer Reviewed
  • [Journal Article] Genetic dissection of silicon uptake ability in rice (Oryza sativa L. ).2006

    • Author(s)
      Wu QS, Wan XY, Su N, Cheng ZJ, Wang JK, Lei CL, Zhang X, Jiang L, Ma JF, Wan JM.
    • Journal Title

      Plant Sci. 171

      Pages: 441-448

    • Peer Reviewed
  • [Journal Article] イネケイ素吸収遺伝子の同定.2006

    • Author(s)
      馬建鋒
    • Journal Title

      ブレインテクノニュース 118

      Pages: 17-21

  • [Journal Article] Plant root responses to three abundant soil mineral: silicon, aluminum and iron.2005

    • Author(s)
      Ma JF.
    • Journal Title

      Crit. Rev. Plant Sci. 24

      Pages: 267-281

    • Peer Reviewed
  • [Journal Article] Identification of silicon form in the xylem of rice (Oryza sativa L. ).2005

    • Author(s)
      Mitani N, Ma JF, Iwashita T.
    • Journal Title

      Plant Cell Physiol. 46

      Pages: 279-283

    • Peer Reviewed
  • [Journal Article] Uptake system of silicon in different plant species.2005

    • Author(s)
      Mitani N, Ma JF.
    • Journal Title

      J. Exp. Bot. 56

      Pages: 1255-1261

    • Peer Reviewed
  • [Journal Article] Functions and transport of silicon in plants.

    • Author(s)
      Ma JF, Yamaji N.
    • Journal Title

      Cellular and Molecular Life Sciences 65

      Pages: 3049-3057

    • Peer Reviewed
  • [Presentation] ブルームレスキュウリ用カボチャ台木のケイ酸吸収特性のさらなる解析.2009

    • Author(s)
      馬建鋒・吾郷幸子・山地直樹・三谷奈見季・岩崎貢三
    • Organizer
      日本土壌肥料学会年会
    • Place of Presentation
      京都
    • Year and Date
      20090915-20090918
  • [Presentation] イネのケイ素分配の分子機構(i).2009

    • Author(s)
      山地直樹・馬建鋒
    • Organizer
      日本土壌肥料学会年会
    • Place of Presentation
      京都
    • Year and Date
      20090915-20090918
  • [Presentation] Silicon uptake and translocation in plants.2009

    • Author(s)
      Ma, J.F.
    • Organizer
      XVI International Plant Nutrition Colloquium 2009
    • Place of Presentation
      California, USA
    • Year and Date
      20090826-20090830
  • [Presentation] Maize silicon transporters.2009

    • Author(s)
      Mitani, N., Yamaji, N., Ma, J.F.
    • Organizer
      XVI International Plant Nutrition Colloquium 2009
    • Place of Presentation
      California, USA
    • Year and Date
      20090826-20090830
  • [Presentation] Transporters involved in uptake and distribution of silicon in rice.2009

    • Author(s)
      Ma, J.F., Yamaji, N., Mitani, N
    • Organizer
      Plant Biology 2009
    • Place of Presentation
      Honolulu, Hawaii
    • Year and Date
      20090718-20090722
  • [Presentation] ケイ酸輸送体Lsi6によるイネ生殖生長期のケイ酸分配.2009

    • Author(s)
      山地直樹・馬建鋒
    • Organizer
      第50回日本植物生理学会年会
    • Place of Presentation
      名古屋
    • Year and Date
      20090321-20090324
  • [Presentation] Silicon Transporters in higher plants.2008

    • Author(s)
      馬建鋒
    • Organizer
      第31回日本分子生物学会年会, 第81回日本生化学学会大会合同大会
    • Place of Presentation
      神戸
    • Year and Date
      20081209-20081212
  • [Presentation] Characterization of silicon transporters from rice and maize.2008

    • Author(s)
      Mitani, N., Yamaji, N., Ma, J.F.
    • Organizer
      Silicon in Agriculture Conference South Africa 2008
    • Place of Presentation
      KwaZulu-Natal, South Africa
    • Year and Date
      20081026-20081031
  • [Presentation] Silicon transporters in rice.2008

    • Author(s)
      Yamaji, N., Mitani, N., Ma, J.F.
    • Organizer
      Silicon in Agriculture Conference South Africa 2008
    • Place of Presentation
      KwaZulu-Natal, South Africa
    • Year and Date
      20081026-20081031
  • [Presentation] Transporters involved in iron acquisition and xylem loading in gramineous plants.2008

    • Author(s)
      Ma, J.F.
    • Organizer
      XIV International Symposium on Iron Nutrition and Interactions in Plants.
    • Place of Presentation
      Beijing, China
    • Year and Date
      20081011-20081016
  • [Presentation] イネのヒ素吸収に関するトランスポーターの同定.2008

    • Author(s)
      馬建鋒・山地直樹・三谷奈見季・Fang-Jie Zhao
    • Organizer
      日本土壌肥料学会年会
    • Place of Presentation
      名古屋
    • Year and Date
      20080909-20080913
  • [Presentation] 異なるイネ科植物由来のケイ酸トランスポーター特性の比較解析.2008

    • Author(s)
      三谷奈見季・千葉由佳子・山地直樹・馬建 鋒
    • Organizer
      日本土壌肥料学会年会
    • Place of Presentation
      名古屋
    • Year and Date
      20080909-20080913
  • [Presentation] Influx and efflux transporters of silicon in higher plants.2008

    • Author(s)
      Ma, J.F.
    • Organizer
      Final Meeting of the DFG-Priority Program 1108. "Dynamics of Plant Membrane Transport"
    • Place of Presentation
      chlob Hirschberg, Germany
    • Year and Date
      20080519-20080521
  • [Presentation] イネのケイ酸トランスポーター.2008

    • Author(s)
      馬建鋒
    • Organizer
      日本農芸化学会
    • Place of Presentation
      名古屋
    • Year and Date
      20080326-20080327
  • [Presentation] イネのヒ素吸収におけるケイ酸輸送体の関与.2008

    • Author(s)
      山地直樹・三谷奈見季・Xiao-Yan Xu・Steve P. McGrath・Fang-Jie Zhao・馬建鋒
    • Organizer
      日本植物生理学会年会
    • Place of Presentation
      札幌
    • Year and Date
      20080320-20080322
  • [Presentation] トウモロコシのケイ酸トランスポーターの同定.2008

    • Author(s)
      三谷奈見季・山地直樹・馬建鋒
    • Organizer
      日本植物生理学会年会
    • Place of Presentation
      札幌
    • Year and Date
      20080320-20080322
  • [Presentation] A unique uptake system of silicon required for high and sustainable production of rice.2007

    • Author(s)
      Ma, J.F.
    • Organizer
      The University of Tokyo International Symposium, Frontier of Microbial and Plant Biotechnology in Environmental and Life Sciences
    • Place of Presentation
      Tokyo
    • Year and Date
      20071205-20071206
  • [Presentation] Influx and efflux transporter genes of silicon in rice.2007

    • Author(s)
      Ma, J.F.
    • Organizer
      The 5th International Symposium of Rice Functional Genomics.
    • Place of Presentation
      Tsukuba, Japan
    • Year and Date
      20071015-20071017
  • [Presentation] トウモロコシ由来のケイ酸吸収遺伝子の単離と解析.2007

    • Author(s)
      三谷奈見季・山地直樹・馬建鋒
    • Organizer
      日本土壌肥料学会年会
    • Place of Presentation
      東京
    • Year and Date
      20070822-20070824
  • [Presentation] A rice aquaporin functions as a silicon transporter.2007

    • Author(s)
      Ma, J.F., Yamaji, N., Mitani, N.
    • Organizer
      The 5th International Conference of Auqporin.
    • Place of Presentation
      Nara, Japan
    • Year and Date
      20070713-20070716
  • [Presentation] Silicon transporters in rice.2007

    • Author(s)
      Ma, J.F., Yamaji, N., Mitani, N.
    • Organizer
      XIV International Workshop on Plant Membrane Biology.
    • Place of Presentation
      Valencia, Spain
    • Year and Date
      20070626-20070630
  • [Presentation] Functional characterization of rice silicon transporter Lsi1.2007

    • Author(s)
      Mitani, N., Ma, J.F.
    • Organizer
      XIV International Workshop on Plant Membrane Biology.
    • Place of Presentation
      Valencia, Spain
    • Year and Date
      20070626-20070630
  • [Presentation] Functional analysis of silicon efflux transporter Lsi2 in rice.2007

    • Author(s)
      Yamaji, N., Mitani, N., Tamai, K., Ma, J.F.
    • Organizer
      XIV International Workshop on Plant Membrane Biology.
    • Place of Presentation
      Valencia, Spain
    • Year and Date
      20070626-20070630
  • [Presentation] イネ科NIPのケイ酸輸送特性の解析.2007

    • Author(s)
      三谷奈見季・山地直樹・且原真木・馬建 鋒
    • Organizer
      日本植物生理学会年会
    • Place of Presentation
      愛媛
    • Year and Date
      20070328-20070330
  • [Presentation] ネケイ酸輸送体Lsi6の機能解析.2007

    • Author(s)
      山地直樹・三谷奈見季・馬建鋒
    • Organizer
      日本植物生理学会年会
    • Place of Presentation
      愛媛
    • Year and Date
      20070328-20070330
  • [Presentation] Silicon transporter genes in rice.2007

    • Author(s)
      Ma, J.F., Yamaji, N., Mitani, N., Tamai, K., Konishi, S., Yano, M.
    • Organizer
      International Plant and Animal Genome Conference XV.
    • Place of Presentation
      San Diego, USA
    • Year and Date
      20070113-20070117
  • [Presentation] 植物のケイ素輸送機構.2007

    • Author(s)
      馬建鋒
    • Organizer
      特定領域研究「植物の養分吸収と循環系」シンポジウム
    • Place of Presentation
      東京大学弥生講堂
    • Year and Date
      2007-09-24
  • [Presentation] イネにおけるケイ素の有益性及び吸収機構に関する研究.2007

    • Author(s)
      馬建鋒
    • Organizer
      日本土壌肥料学会賞記念講演
    • Place of Presentation
      東京
    • Year and Date
      2007-08-23
  • [Presentation] イネケイ酸吸収遺伝子Lsi1の輸送特性の解析.2006

    • Author(s)
      三谷奈見季・且原真木・馬建鋒
    • Organizer
      日本土壌肥料学会年会
    • Place of Presentation
      秋田
    • Year and Date
      20060905-20060907
  • [Presentation] イネの根におけるケイ酸吸収関連遺伝子の分布と発現解析.2006

    • Author(s)
      山地直樹・馬建鋒
    • Organizer
      日本土壌肥料学会
    • Place of Presentation
      秋田
    • Year and Date
      20060905-20060907
  • [Presentation] イネ新規ケイ酸吸収関連遺伝子Lsi2のクローニング.2006

    • Author(s)
      玉井一規・小西左江子・矢野昌裕・馬建 鋒
    • Organizer
      日本植物生理学会
    • Place of Presentation
      つくば
    • Year and Date
      20060319-20060321
  • [Presentation] イネケイ酸吸収関連遺伝子Lsi2の機能解析.2006

    • Author(s)
      山地直樹・馬建鋒
    • Organizer
      日本植物生理学会年会
    • Place of Presentation
      茨城
    • Year and Date
      20060319-20060321
  • [Presentation] イネのケイ酸吸収を司る遺伝子の単離と解析.2006

    • Author(s)
      馬建鋒
    • Organizer
      イネゲノム解読記念シンポジウム-イネゲノム解読でなにができるか?-
    • Place of Presentation
      つくば
    • Year and Date
      2006-03-22
  • [Presentation] Roles of an NIP gene in silicon transporter in rice.2006

    • Author(s)
      Ma, J.F.
    • Organizer
      植物生理学会年会シンポジウム, Plant NIP-their structure function analysis and roles in plant physiolog
    • Place of Presentation
      つくば
    • Year and Date
      2006-03-21
  • [Presentation] Uptake and translocation of silicon in higher plants.2005

    • Author(s)
      Ma, J.F., Yamaji, N., Mitani, N., Tamai, K., Konishi, S., Iwashita, T., Yano, M.
    • Organizer
      Pacifichem
    • Place of Presentation
      Hawaii, United State
    • Year and Date
      20051215-20051220
  • [Presentation] Silicon requirement for rice.2005

    • Author(s)
      Ma, J.F.
    • Organizer
      IIISilicon in Agriculture Conference
    • Place of Presentation
      Uberlandia Brazil
    • Year and Date
      20051022-20051026
  • [Presentation] Isolation and characterization of novel rice mutant deffective in Si uptake.2005

    • Author(s)
      Tamai, K., Takeoka, Y., Konishi, S., Mitani, N., Yano, M., Ma, J.F.
    • Organizer
      IIISilicon in Agriculture Conference
    • Place of Presentation
      Uberlandia Brazil
    • Year and Date
      20051022-20051026
  • [Presentation] Uptake system of silicon in different plant species.2005

    • Author(s)
      Mitani, N., Ma, J.F.
    • Organizer
      XV International Plant Nutrition Colloquium
    • Place of Presentation
      Beijing, China
    • Year and Date
      20050914-20050919
  • [Presentation] 新規イネケイ酸吸収関連遺伝子Lsi2の単離と解析2005

    • Author(s)
      玉井一規, 三谷奈見季, 山地直樹, 小西左江子, 矢野昌裕, 馬建鋒
    • Organizer
      日本土壌肥料学会年会
    • Place of Presentation
      島根
    • Year and Date
      20050906-20050909
  • [Presentation] Isolation and functional analysis of genes controlling Si uptake in rice.2005

    • Author(s)
      Ma, J.F., Tamai, K., Yamaji, N., Mitani, N., Konishi, S., Yano, M.
    • Organizer
      10th International Congress of SABRAO
    • Place of Presentation
      Tsukuba, Japan
    • Year and Date
      20050822-20050823
  • [Presentation] Isolation and characterization of Lsi12005

    • Author(s)
      Mitani, N., Tamai, K., Konishi, S., Yano, M., Yamaji, N., Kyo, M., Ma, J.F.
    • Organizer
      日本植物生理学会年会
    • Place of Presentation
      新潟
    • Year and Date
      20050324-20050326
  • [Presentation] Characterization of a novel low Si rice mutant (lsi2) and molecular mapping of responsible gene in rice2005

    • Author(s)
      玉井一規, 武岡祐子, 小西左江子, 矢野昌裕, 藤原徹, 馬建鋒
    • Organizer
      日本植物生理学会年会
    • Place of Presentation
      新潟
    • Year and Date
      20050324-20050326
  • [Presentation] Silicon and stress tolerance in plants.2005

    • Author(s)
      Ma, J.F.
    • Organizer
      Agricultural Plant Stress Response V
    • Place of Presentation
      Gwangju, Korea
    • Year and Date
      2005-09-29
  • [Book] In Handbook of Biomineralization (Baeuerlein e. Ed, Silicon uptake in different plant species)2007

    • Author(s)
      Ma JF.
    • Total Pages
      113-124
    • Publisher
      WILEY-VCH
  • [Book] IIイネのケイ酸要求性と吸収特性. イネの生産性・品質と栄養生理.2006

    • Author(s)
      馬建鋒
    • Total Pages
      37-54
    • Publisher
      博友社
  • [Remarks] 朝日新聞、読売新聞、山陽新聞など研究成果の報道があった。

  • [Patent(Industrial Property Rights)] ケイ素吸収に関与する遺伝子の利用2007

    • Inventor(s)
      馬建鋒
    • Industrial Property Rights Holder
      馬建鋒
    • Industrial Property Number
      特許2007-065580
    • Filing Date
      2007-03-14
  • [Patent(Industrial Property Rights)] ケイ素吸収に関与する遺伝子、及びその利用2004

    • Inventor(s)
      馬建鋒
    • Industrial Property Rights Holder
      馬建鋒
    • Industrial Property Number
      特許2004-381899
    • Filing Date
      2004-12-28
  • [Patent(Industrial Property Rights)] ケイ素吸収に関与する遺伝子、およびその利用

    • Inventor(s)
      馬建鋒
    • Industrial Property Rights Holder
      馬建鋒
    • Industrial Property Number
      特許2005-238030

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Published: 2011-06-18   Modified: 2016-04-21  

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