Regulation mechanism of biosynthesis, intracellular localization and degradation of membrane transporters

2009 Fiscal Year Final Research Report

• Project/Area Number: 17078009
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Kyushu University (2007-2009); The Institute of Physical and Chemical Research (2005-2006)
• Principal Investigator: MATSUOKA Ken Kyushu University, 大学院・農学研究院, 教授 (40222294)
• Co-Investigator(Kenkyū-buntansha): TOYOOKA Kiminori 独立行政法人理化学研究所, 機能開発研究グループ, 研究員 (10360596)
• Project Period (FY): 2007 – 2009

Research Abstract

We investigated the regulation of autophagic degradation as well as the intracellular localization and degradation of tobacco SCAMP, which is known as an escort protein of membrane transporters, as well as sucrose transporters from tobacco and a borate transporter from Arabidopsis. We found a novel intracellular structure through the analysis of the SCAMP. We also got novel mechanistic insights on the nutritional regulation of the degradation of both sucrose transporters and the borate transporter.

Research Products

• [Journal Article] Closing plant stomata requires a homolog of an aluminum-activated malate transporter. (2010)
  • Author(s): Sasaki, T., Mori, I., Furuichi, T., Munemasa, S., Toyooka, K., Matsuoka, K., Murata, Y. Yamamoto, Y.
  • Journal Title: Plant Cell Physiol. 51 Pages: 354-365
• [Journal Article] Proline and glycinebetaine induce antioxidant defense gene expression and suppress cell death in cultured tobaccc cells under salt stress. (2009)
  • Author(s): Banu, M.N., Hogue, M.A., Watanabe祐ugimoto, M., Matsuoka, K, Nakamura, Y., Shimoisli, Y., Murata, Y.
  • Journal Title: J Plant Physiol. 166 Pages: 146-156
• [Journal Article] MATE-type Transporters Implicated in Vacuolar Sequestration of Nicotine in Tobacco Roots. (2009)
  • Author(s): Shoji, T., Inai, K., Yazaki, Y., Sato, Y., Takase, H., Shitan, N., Yazaki, K., Goto, Y., Toyooka, K., Matsuoka, K., Hashimoto, T.
  • Journal Title: Plant Physiol 149 Pages: 708-718
• [Journal Article] Preferential up羊egulation of G2/M phasmspecific genes by overexpression ofiyperactive form of NtmybA2 lacking its negative regulation domain in tobacco BY2 cells. (2009)
  • Author(s): Suzuki, S., Galis, I., Kato, K., Araki, S., Demura, T., Criqui, M-C., Potuschak, T., Genschik, P., Matsuoka, K., Ito, M.
  • Journal Title: Plant Physiol. 149 Pages: 1945-1957
• [Journal Article] Identification and characterization of the Na+/H+ antiporter NHAS3 from the thylakoid membrane of synechocystis SP. PCC 6803. (2009)
  • Author(s): Tsunekawa K, Shi juku T, Hayashimoto M, Kojima Y, Onai K, Morishita M, Ishiura M, Kuroda T, Nakamura T, Kobayashi H, Sato M, Toyooka K, Matsuoka K, Omata T, Uozumi N.
  • Journal Title: J. Biol. Chem. 284 Pages: 16513-16521
• [Journal Article] A mobile secretory vesicle cluster involved in mass transport from the Golgi to plant cell exterior. (2009)
  • Author(s): Toyooka, K., Goto, Y., Asatsuma, S., Koizumi, M., Mitsui, T. Matsuoka, K.
  • Journal Title: Plant Cell 21 Pages: 1212-1229
• [Journal Article] Plastid targeting of a-amylase glycoprotein from the Golgi apparatus through the secretory pathway. (2009)
  • Author(s): Kitajima, A., Asatsuma, S., Okada, H., Hamada, Y., Kanekoa, K., Nan joa, Y., Kawagoe, Y., Toyooka, K., Matsuoka, Takeuchi, M., Nakano, A., Mitsui, T
  • Journal Title: Plant Cell 21 Pages: 2844-2858
• [Journal Article] Exo- and Endocytotic trafficking of SCAMP2. (2009)
  • Author(s): Toyooka, K., Matsuoka, K.
  • Journal Title: Plant Signaling and Behayior 4 Pages: 1196-1198
• [Journal Article] Characterization of a Tobacco TPK-type K+ Channel as a Novel Tonoplast K+ Channel Using Yeast Tonoplasts. (2008)
  • Author(s): Hamamoto, S., Marui, J., Matsuoka, K., Higasihi, K., Igarashi, K., Nakagawa, T., Mori, Y., Murata, Y., Nakanishi, Y., Maeshima, M., Yabe, I., Uozumi, N.
  • Journal Title: J. Biol. Chem. 283 Pages: 1911-1920
• [Journal Article] Guidelines for the Use and Interpretation of Assays for Monitoring Autophagy in Higher Eukaryot es. (2008)
  • Author(s): Klionsky, et al., 226人中127番目
  • Journal Title: Autophagy 4 Pages: 151-175
• [Journal Article] Characterization of NtChitIV, a class IV chitinase induced by-5, 3-, 1, 6-glucan elicitor fromAlternaria alternata 102: Antagonistic effect of salicylic acid and methyl jasmonate on the induction of NtChitIV. (2007)
  • Author(s): Shinya, T., Hanai, K., Suzuki, K., Matsuoka, K., Matsuoka, H., Saito, M.
  • Journal Title: Biochem. Biophys. Res. Commun. 353 Pages: 311-317
• [Journal Article] Novel regulation of MHC class II function in B cells. (2007)
  • Author(s): Matsuki, Y., Ohmura-Hoshino, M.,, Goto, E., Aoki, M., MitnYoshida, M., Uematsu, M., Hasegawa, T., Koseki, H., Ohara, O, Nakayama, M., Toyooka, K., Matsuoka, K., Hotta, H., Yamamoto, A., IshidoS.
  • Journal Title: EMBO J. 26 Pages: 846-854
• [Journal Article] Development of the series of gateway binary vectors, pGWB, realize efficient construction of fusion genes for plant transformation. (2007)
  • Author(s): Nakagawa, T., Kurose, T., Hino, T., Tanaka, K., Kawamukai, M.,, Niwa, Y., Tovooka, K., Matsuoka, K., Jinbo, T., Kimura, T.
  • Journal Title: J. Biosci. Bioeng. 104 Pages: 34-41
• [Journal Article] Comprehensive analysis of glucan elicitor-regulated gene expression in tobacco BY-2 cells revealsa novel MYB transcription factor involved lithe regulation of phenylpropanoid metabolism. (2007)
  • Author(s): Shinya, T., Galis, I., Narisawa, T., Sasaki, M., Fukuda, H., Matsuoka, H., Saito, M., Matsuoka, K.
  • Journal Title: Plant Cell Physiol. 48 Pages: 1404-1413
• [Journal Article] Cytological and Biochemical Analysis of COF1, an Arabidopsis Mutant of an ABC Transporter Gene. (2007)
  • Author(s): Ukitsu, H., Kuromori, T., Toyooka, K., Goto, Y., Matsuoka, K., Sakuradani, E., Shimizu, S., Kamiya, A., Imura, Y., Yuguchi, M., Wada, T., Hirayama, T., Shinozaki, K.
  • Journal Title: Plant Cell Physio1. 48 Pages: 1524-1533
• [Journal Article] Protein aggregates are transported to vacuoles by macroautophagic mechanism in nutrient-starved plant cells. (2006)
  • Author(s): Toyooka, K., Takeuchi, M., Moriyasu, Y., Fukuda, H., Matsuoka, K.
  • Journal Title: Autophagy 2 Pages: 91-106
• [Journal Article] A novel R2R3 MYB transcription factor NtMYBJS1 is a methyl jasmonate-dependent regulator of phenylpropanoid-conjugate biosynthesis in tobacco (2006)
  • Author(s): Galls, I., Simek, P., Narisawa, T., Sasaki, M., Horiguchi, T., Fukuda, H., Matsuoka, K.
  • Journal Title: Plant J. 46 Pages: 573-592
• [Journal Article] Expression and Ca2+ Dependency of Plasma Membrane K+ Channels of Tobacco Suspension Cells Adapted to Salt Stress (2006)
  • Author(s): Kasukabe, N., WatanabeSugimoto, M., Matsuoka, K., Okuma, E., Obi, I., Nakamura, Y., Shimoishi, Y., Murata, Y., Kakutani, K.
  • Journal Title: Plant Cell Physio1. 47 Pages: 1674-1677
• [Presentation] Localization and trafficking of multispanning membrane proteins at the transGolgi network and subsequent secretory orgqanelle. (2010)
  • Author(s): Matsuoka, K
  • Organizer: International Symposium on Plant Membrane Transport.
  • Place of Presentation: Tokyo
  • Year and Date: 20100000
• [Presentation] 植物細胞における液胞形成機構の細胞生物学的解析 (2010)
  • Author(s): 佐藤繭子、後藤友美、豊岡公徳、松岡健
  • Organizer: 第51回口本植物生理学会年会
  • Place of Presentation: 熊本
  • Year and Date: 20100000
• [Presentation] 光混合栄養生長に必要なSynechocysitis sp PCC6803アクアポリンの解析 (2010)
  • Author(s): 赤井政郎、小内清、森下めぐみ、草野都、佐藤繭子、Henning Redesting小林誠、大槻瞳、豊岡公徳、松岡健、斎藤和季、石浦正寛、魚住信之
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本
  • Year and Date: 20100000
• [Presentation] 後期分泌系構造体Secretory Vesicle Clusterに含まれる蛋白質の解析 (2010)
  • Author(s): 林恭子、平田励、浅妻悟、豊岡公徳、松岡健
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本
  • Year and Date: 20100000
• [Presentation] タバコ培養細胞におけるショ糖輸送体の局在と分解 (2010)
  • Author(s): 浅妻悟、豊岡公徳、後藤友美, 松岡健
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本
  • Year and Date: 20100000
• [Presentation] 高圧凍結技法による単膜系オルガネラの網羅的解析:根冠組織 (2010)
  • Author(s): 豊岡公徳、後藤友美、佐藤繭子、松岡健
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本
  • Year and Date: 20100000
• [Presentation] ゴルジ装置と細胞増殖:細胞壁成分合成系の局在と細胞外への大量輸送装置 (2009)
  • Author(s): 松岡健、豊岡公徳、浅妻悟、森口亮
  • Organizer: 第82回日本生化学会大会シンポジウム2Sa14a植物の成長生理とメンブレントラフィック
  • Place of Presentation: 神戸
  • Year and Date: 20090000
• [Presentation] タバコ培養細胞におけるショ糖輸送体の細胞内局在と栄養飢餓による分解制御の解析 (2009)
  • Author(s): 浅妻悟、豊岡公徳、後藤友美、松岡健
  • Organizer: 日本農芸化学会2009年度大会
  • Place of Presentation: 福岡
  • Year and Date: 20090000
• [Presentation] 後期分泌系構造体Secretory Vesicle Clusterの単離と含まれる蛋白質の解析 (2009)
  • Author(s): 平田励、浅妻悟、豊岡公徳、松岡健
  • Organizer: 日本農芸化学会2009年度大会
  • Place of Presentation: 福岡
  • Year and Date: 20090000
• [Presentation] RlR2R3-Myb転写活性化因子を過剰発現するZBY2細胞のトランスクリプトーム解析 (2009)
  • Author(s): 加藤貴一、IvanGalis、鈴木しをり、松岡、伊藤正樹
  • Organizer: 日本植物生理学会創立50周年記念年会
  • Place of Presentation: 名古屋
  • Year and Date: 20090000
• [Presentation] 高圧凍結技法を用いた液胞形成機構の免疫組織化学的解析 (2009)
  • Author(s): 豊岡公徳、後藤友美、佐藤繭子、黒森崇、吉本光希、前島正義、松岡健
  • Organizer: 日本植物生理学会創立50周年記念年会
  • Place of Presentation: 名古屋
  • Year and Date: 20090000
• [Presentation] Secretory vesicle clusterを介した分泌機構の解析 (2008)
  • Author(s): 豊岡公徳, 後藤友美, 佐藤繭子, 松岡健
  • Organizer: 第49回日本植物生理学会年会
  • Year and Date: 20080300
• [Presentation] Secretory vesicle cluster: a Golgi-to-plasma membrane transport compartment containing SCAMP2 in tobacco cell- (2008)
  • Author(s): Ken Matsuoka
  • Organizer: Invited Seminar at Chinese University of Hong Kong
  • Place of Presentation: Hong Kong
  • Year and Date: 20080000
• [Presentation] Secretory Vesicle Cluster:トランスゴルジネットワーク由来の分泌系後期で働く新奇構造体 (2008)
  • Author(s): 松岡健, 豊岡公徳, 浅妻悟, 後藤友美, 三ツ井敏明
  • Organizer: 第49回日本植物生理学会シンポジウム「小胞輸送研究の新展開:構造・動きと局在制御の分子機構」
  • Place of Presentation: 札幌
  • Year and Date: 20080000
• [Presentation] イネ糖タンパク質α一アミラーゼ1-1のゴルジ体-プラスチド間輸送に関する研究 (2008)
  • Author(s): 北島彩、岡田久夫、浜田侑紀、豊岡公徳、浅妻悟、松岡健、中野明彦、三ツ井敏明
  • Organizer: BMB2008(第31回日本分子生物学会第81回日本生化学会合同大会)
  • Place of Presentation: 神戸
  • Year and Date: 20080000
• [Presentation] タバコ培養細胞の糖処理におけるショ糖輸送体の細胞内局在と発現調節 (2008)
  • Author(s): 浅妻悟, 後藤友美, 豊岡公徳, 松岡健
  • Organizer: 第49回日本植物生理学会年会
  • Place of Presentation: 札幌
  • Year and Date: 20080000
• [Presentation] 豊岡公徳植物組織凍結超薄ライブラリーの作製とその免疫電子顕微鏡観察法の検討 (2008)
  • Author(s): 佐藤繭子, 後藤友美, 松岡健, 篠崎一雄
  • Organizer: 第49回日本植物生理学会年会
  • Place of Presentation: 札幌
  • Year and Date: 20080000
• [Presentation] イネ糖タンパク質α-アミラーゼ1-1のプラスチドターゲティング:ゴルジープラスチド間輸送の解析 (2008)
  • Author(s): 北嶋彩, 唐橋あゆみ, 高田頌, 豊岡公徳, 浅妻悟, 松岡健, 中野明彦, 三ツ井敏明
  • Organizer: 第49回日本植物生理学会年会
  • Place of Presentation: 札幌
  • Year and Date: 20080000
• [Presentation] Protective roles of proline and glycinebetaine in NaCl-induced cell death in cultured tobacco cells (2008)
  • Author(s): Mst. Nasrin Akhter BANU, Md. Anamul HOQUE, Megumi WATANABE-SUGIMOTO, Ken MATSUOKA, Yoshimasa NAKAMURA, Yasuaki SHIMOISHI, Yoshiyuki MURATA
  • Organizer: 日本農芸化学会2008年度大会
  • Place of Presentation: 名古屋
  • Year and Date: 20080000
• [Presentation] Characterization of a tobacco secretory carreier membrane protein 2 in tobacco BY-2 cells reveals a novel exocytotic mobile unit, secretory vesicle cluster. (2007)
  • Author(s): Matsuoka, K., Toyooka, K., Goto, Y., Asatsuma, S
  • Organizer: XIVth International Workshop on Plant Membrane Biology.
  • Place of Presentation: Valencia, Spain
  • Year and Date: 20070000
• [Presentation] Transcriptomic and cell biological analyses of tobacco cell line BY-2. (2007)
  • Author(s): Matsuoka, K
  • Organizer: Japan-Germany International Cooperative Project on Education and Research Workshop "Multi-functionality and Sustainability of Land Use in SE. and E. Asia7
  • Place of Presentation: Fukuoka, Japan
  • Year and Date: 20070000
• [Presentation] 蛍光モデルタンパク質とタバコcDNAマイクロアレイを用いた植物細胞の窒素欠乏応答の解析 (2007)
  • Author(s): 松岡健
  • Organizer: 2007年度土壌肥料学会九州支部春期例会特別講演
  • Place of Presentation: 福岡
  • Year and Date: 20070000
• [Presentation] 植物分泌系における4回膜貫通タンパク質の輸送と局在 (2007)
  • Author(s): 松岡健
  • Organizer: 公開シンポジウム「植物膜輸送」の最前線
  • Place of Presentation: 東京
  • Year and Date: 20070000
• [Presentation] Nobuyuki Uozumi, Functional expression of tobacco K+ channels in yeast tonoplast (2007)
  • Author(s): Shin Hamamoto, Junichiro Marui, Ken Matsuoka, Tetsuro Mimura, Tsuyoshi Nakagawa, Yoshiyuki Murata, Yoichi Nakanishi, Masayoshi Maeshima, Isamu Yabe
  • Organizer: XIV International workshop on plant membrane biology.
  • Place of Presentation: Valencia, Spain
  • Year and Date: 20070000
• [Presentation] 輸送体の局在変化による養分吸収・分配の制御と養分欠乏が誘導する分解系 (2006)
  • Author(s): 松岡健
  • Organizer: 九州大学農学研究院植物資源科学部門第1回専攻セミナー
  • Place of Presentation: 福岡
  • Year and Date: 20060000
• [Presentation] タバコ細胞における分泌系オルガネラ解析の為の複数回膜貫通蛋白質の局在と動態の解析
  • Author(s): 松岡健、後藤友美、豊岡公徳、浅妻悟
  • Organizer: BMB2008(第31回日本分子生物学会第81回日本生化学会合同大会)
• [Book] Chimeric Fluorescent Fusion Proteins to Monitor Autophagy Plants" in Meth. Enzymol (2009)
  • Author(s): Matsuoka, K.
  • Total Pages: 541-555
• [Book] Plant Cell Monographs (2006)
  • Author(s): Aniento, F., Matsuoka, K., Robinson, D.G.
  • Total Pages: 99-124
• [Book] Tobacco BY-2 Cells: From Cellular Dynamics to Omics. (2006)
  • Author(s): Toshiyuki Nagata, Ken Matsuoka, Dirk Inze
• [Book] Autophagy and non-classical vacuolar targeting in tobacco BY-2 cells (2006)
  • Author(s): Toyooka, K., Matsuoka, K
  • Total Pages: 167-180
  • Publisher: Springer-Verlag
• [Book] EST and microarray analysis of tobacco BY-2 cells" in "Tobacco BY-2 Cells: From Cellular Dynamics to Omics (2006)
  • Author(s): Matsuoka, K., Galis, I
  • Total Pages: 293-311
  • Publisher: Springer-Verlag
• [Book] High throughput microinjection technology for the single-cell analysis of BY-2 in vivo (2006)
  • Author(s): Matsuoka, H., Yamada, Y., Matsuoka, Saito. M.
  • Total Pages: 339-346
  • Publisher: Springer-Verlag