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2009 Fiscal Year Final Research Report

Functional analysis of atypical G proteins involved in cell signaling network

Research Project

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Project/Area Number 17079002
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionThe University of Tokyo

Principal Investigator

KATADA Toshiaki  The University of Tokyo, 大学院・薬学系研究科, 教授 (10088859)

Co-Investigator(Kenkyū-buntansha) KONTANI Kenji  東京大学, 大学院・薬学系研究科, 准教授 (30302615)
KAJIHO Hiroaki  東京大学, 大学院・薬学系研究科, 助教 (70401221)
FUKUYAMA Masamitsu  東京大学, 大学院・薬学系研究科, 助教 (20422389)
KOBAYASHI Tetsuo  東京大学, 大学院・薬学系研究科, 助教 (80433994)
SAITO Kota  東京大学, 大学院・薬学系研究科, 助教 (60549632)
Project Period (FY) 2005 – 2009
Keywordsタンパク質 / 遺伝子 / シグナル伝達 / G蛋白質 / 小胞輸送 / リソソーム
Research Abstract

G proteins, which cycle between the two different GTP- and GDP-bound conformations, play important roles as a "molecular switch" in many intracellular signaling pathways. The G protein families include the translation factors, the trimeric G proteins, and the small GTPases. Although the small GTPases are involved in the regulation of cell growth/differentiation, vesicle trafficking, and cell shape/adhesion, there are still many other GTPases, of which functions are unknown. In the Scientific Research on Priority Areas "G-protein signal", we investigated novel GTPases exhibiting unique biochemical and/or structural properties different from typical small GTPases and found that the atypical G proteins play important roles in lysosome biogenesis, cilia formation, and recycling-endosome dynamics. Thus, our findings contributed much toward an understanding of the novel functions of G proteins.

  • Research Products

    (38 results)

All 2010 2009 2008 2007 2006 2005 Other

All Journal Article (21 results) (of which Peer Reviewed: 17 results) Presentation (15 results) Remarks (2 results)

  • [Journal Article] The Arf-like GTPase Arl8 mediates delivery of endocytosed macromolecules to lysosomes in Caenorhabditis elegans. Mol. Biol. Cell2010

    • Author(s)
      Nakae I, Fujino T, Kobayashi T, Sasaki A, Kikko Y, Fukuyama M, Gengyo-Ando K, Mitani S, Kontani K, Katada T.
    • Journal Title

      Mol. Biol. Cell (in press)

    • Peer Reviewed
  • [Journal Article] Blacque OE. Joubert syndrome Arl13b functions at ciliary membranes and stabilizes protein transport in Caenorhabditis elegans.2010

    • Author(s)
      Cevik S, Hori Y, Kaplan OI, Kida K, Toivenon T, Foley-Fisher C, Cottell D, Katada T, Kontani K
    • Journal Title

      J. Cell Biol. 188(6)

      Pages: 953-969

    • Peer Reviewed
  • [Journal Article] Filamin associates with stress signaling kinases MKK7 and MKK4 and regulates JNK activation.2010

    • Author(s)
      Nakagawa K, Sugahara M, Yamasaki T, Kajiho H, Takahashi S, Hirayama J, Minami Y, Ohta Y, Watanabe T, Hata Y, Katada T, Nishina H.
    • Journal Title

      Biochem J. 427(2)

      Pages: 237-245

    • Peer Reviewed
  • [Journal Article] gタンパク質研究の動向2010

    • Author(s)
      堅田利明
    • Journal Title

      細胞(The Cell) 42(3)

      Pages: 90-91

  • [Journal Article] アミノ酸シグナル伝達に介在するgタンパク質Rag2010

    • Author(s)
      春日秀文、福山征光、紺谷圏二、堅田利明
    • Journal Title

      細胞(The Cell) 42(3)

      Pages: 108-111

  • [Journal Article] Arf-like protein 13B.2009

    • Author(s)
      Kontani K, Hori Y, Katada T.
    • Journal Title

      Nature Molecule Pages, Published online

    • Peer Reviewed
  • [Journal Article] Biochemical characterization of missense mutations in the Arf/Arl-family small GTPase Arl6 causing Bardet-Biedl syndrome.2009

    • Author(s)
      Kobayashi T, Hori Y, Ueda N, Kajiho H, Muraoka S, Shima F, Kataoka T, Kontani K, Katada T.
    • Journal Title

      Biochem. Biophys. Res. Commun. 381(3)

      Pages: 439-442

    • Peer Reviewed
  • [Journal Article] Upf1 potentially serves as a RING-related E3 ubiquitin ligase via its association with Upf3 in yeast.2008

    • Author(s)
      Takahashi S, Araki Y, Ohya Y, Sakuno T, Hoshino S, Kontani K, Nishina H, Katada T.
    • Journal Title

      RNA 14

      Pages: 1950-1958

    • Peer Reviewed
  • [Journal Article] Domain architecture of the atypical Arf-family GTPase Arl13b involved in cilia formation.2008

    • Author(s)
      Hori Y, Kobayashi T, Kikko Y, Kontani K, Katada T.
    • Journal Title

      Biochem. Biophys. Res. Commun. 373

      Pages: 119-124

    • Peer Reviewed
  • [Journal Article] Tyr-phosphorylation signals translocate RIN3, the small GTPase Rab5-GEF, to early endocytic vesicles.2008

    • Author(s)
      Yoshikawa M, Kajiho H, Sakurai K, Minoda T, Nakagawa S, Kontani K, Katada T.
    • Journal Title

      Biochem. Biophys. Res. Commun. 372

      Pages: 168-172

    • Peer Reviewed
  • [Journal Article] Mechanism of mRNA deadenylation: evidence for a molecular interplay between translation termination factor eRF3 and mRNA deadenylases.2007

    • Author(s)
      Funakoshi Y, Doi Y, Hosoda N, Uchida N, Osawa M, Shimada I, Tsujimoto M, Suzuki T, Katada T, Hoshino S.
    • Journal Title

      Genes Dev. 23

      Pages: 3135-3148

    • Peer Reviewed
  • [Journal Article] Activation of the c-Jun N-terminal kinase pathway by MST1 is essential and sufficient for the induction of chromatin condensation during apoptosis.2007

    • Author(s)
      Ura S, Nishina H, Gotoh Y, Katada T.
    • Journal Title

      Mol Cell Biol. 27

      Pages: 5514-5522

    • Peer Reviewed
  • [Journal Article] Characterization of Rab45/RASEF containing EF-hand domain and a coiled-coil motif as a self-associating GTPase.2007

    • Author(s)
      Shintani M, Tada M, Kobayashi T, Kajiho H, Kontani K, Katada T.
    • Journal Title

      Biochem Biophys Res Commun. 357

      Pages: 661-667

    • Peer Reviewed
  • [Journal Article] GTP binding is essential to the protein kinase activity of LRRK2, a causative gene product for familial Parkinson's disease.2007

    • Author(s)
      Ito G, Okai T, Fujino G, Takeda K, Ichijo H, Katada T, Iwatsubo T.
    • Journal Title

      Biochemistry 46

      Pages: 1380-1388

    • Peer Reviewed
  • [Journal Article] Role of activation of PIP5Kgamma661 by AP-2 complex in synaptic vesicle endocytosis.2007

    • Author(s)
      Nakano-Kobayashi A, Yamazaki M, Unoki T, Hongu T, Murata C, Taguchi R, Katada T, Frohman MA, Yokozeki T, Kanaho Y.
    • Journal Title

      EMBO J. 26

      Pages: 1105-1116

    • Peer Reviewed
  • [Journal Article] Caf1 regulates translocation of ribonucleotide reductase by releasing nucleoplasmic Spd1-Suc22 assembly.2007

    • Author(s)
      Takahashi S, Kontani K, Araki Y, Katada T.
    • Journal Title

      Nucleic Acid Res. 35

      Pages: 1187-1197

    • Peer Reviewed
  • [Journal Article] 低分子量gタンパク質研究の進歩2007

    • Author(s)
      多田稔、小林哲夫、紺谷圏二、堅田利 明
    • Journal Title

      日薬理誌 130

      Pages: 373-379

  • [Journal Article] Release of RASSF1C from the nucleus by Daxx degradation links DNA damage and SAPK/JNK activation.2006

    • Author(s)
      Kitagawa D, Kajiho H, Negishi T, Ura S, Watanabe T, Wada T, Ichijo H, Katada T, Nishina H.
    • Journal Title

      EMBO J. 25

      Pages: 3286-3297

    • Peer Reviewed
  • [Journal Article] The decapping enzyme Dcp1 participates in translation termination through its interaction with the release factor eRF3 in budding yeast. Biochem.2006

    • Author(s)
      Kofuji S, Sakuno T, Takahashi S, Araki Y, Doi Y, Hoshino S, Katada T.
    • Journal Title

      Biophys. Res. Commun. 344

      Pages: 547-553

    • Peer Reviewed
  • [Journal Article] Purification and analysis of RIN family - novel Rab5 GEFs.2005

    • Author(s)
      Saito K, Kajiho H, Araki Y, Kurosu H, Kontani K, Nishina H, Katada T.
    • Journal Title

      Methods Enzymol. 403

      Pages: 276-283

    • Peer Reviewed
  • [Journal Article] Novel role of the small GTPase Rheb: Its implication in endocytic pathway independent of the activation of mammalian target of rapamycin.2005

    • Author(s)
      Saito K, Araki Y, Kontani K, Nishina H, Katada T.
    • Journal Title

      J. Biochem. 137

      Pages: 423-430

  • [Presentation] 後期エンドソームとリソソームの融合・再形成過程に介在する低分子量g蛋白質Arl8(シンポジウム講演)2009

    • Author(s)
      堅田利明、中江郁青、菊香順史、紺谷圏 二
    • Organizer
      第82回日本生化学会大会
    • Place of Presentation
      神戸
    • Year and Date
      2009-10-23
  • [Presentation] The Arf-like GTPase Arl8 is required for lysosome biogenesis in C. elegans.2009

    • Author(s)
      Katada T, Kontani K
    • Organizer
      IUPS 2009: PSJ Symposium, VI-5; Recent progress on G-protein signalings
    • Place of Presentation
      Kyoto, Japan
    • Year and Date
      2009-07-28
  • [Presentation] The Arf-like GTPase ARL-8 is required for lysosome biogenesis in C. elegans2009

    • Author(s)
      Nakae I, Fujino T, Kobayashi T, Kikko Y, Harada S, Fukuyama M, Gengyo-, o K, Mitani S, Kontani K, Katada T
    • Organizer
      17th International C. elegans Meeting
    • Place of Presentation
      Los Angeles, CA, USA
    • Year and Date
      2009-06-27
  • [Presentation] Distinct control of survival, and somatic and germline development during L1 diapause by the insulin/IGF signaling and AMPK pathway2009

    • Author(s)
      Fukuyama M, Sakuma K, Astumi Y, Shimomura H, Kasuga H, Rougvie A, Katada T
    • Organizer
      17th International C. elegans Meeting
    • Place of Presentation
      Los Angeles, CA, USA
    • Year and Date
      2009-06-26
  • [Presentation] Nakagawa S, Kontani K, Katada T.: Tyrphosphorylation signals translocate RIN3, the small GTPase Rab5-GEF, to early endocytic vesicles.2008

    • Author(s)
      Kajiho H, Yoshikawa M, Sakurai K, Minoda T, Nakagawa S, Kontani K, Katada T.
    • Organizer
      48th ASCB Annual Meeting
    • Place of Presentation
      米国、サンフランシスコ
    • Year and Date
      20081200
  • [Presentation] 低分子量g蛋白質Arl8の神経細胞における機能解析(口頭発表)2008

    • Author(s)
      中江郁青、安藤恵子、三谷昌平、紺谷圏 二、堅田利明
    • Organizer
      ファーマ・バイオフォーラム2008
    • Place of Presentation
      東京
    • Year and Date
      20081100
  • [Presentation] 低分子量g蛋白質Rhoファミリーは新規細胞内構造体P-bodyの形成を制御する(ポスター発表)2008

    • Author(s)
      櫻井京子、高橋真也、蛯原有沙、梶保博 昭、仁科博史、堅田利明
    • Organizer
      文部科学省特定領域研究「細胞情報ネットワークを統合するg蛋白質シグナル研究の新展開
    • Place of Presentation
      湯沢
    • Year and Date
      20080900
  • [Presentation] 細胞のシグナル伝達系に介在するgタンパク質:三量体gタンパク質Giの発見からgタンパク質が果たす役割の拡大に向けて(特別講演)2008

    • Author(s)
      堅田利明
    • Organizer
      産業医科大学大学院イニシアティブワークショップ2008
    • Place of Presentation
      北九州
    • Year and Date
      20080300
  • [Presentation] Functional analysis of the Arf-like small GTPase Arl8 in lysosomesシンポジウム「Membrane Traffic and Organellar Functions」2007

    • Author(s)
      紺谷圏二、藤野知子、小林哲夫、中江郁 青、菊香順史、原田さやか、安藤恵子、三谷昌平、堅田利明
    • Organizer
      第30回日本分子生物学会・第80回日本生化学会合同大会
    • Place of Presentation
      横浜
    • Year and Date
      20071200
  • [Presentation] Control of developmental quiescence and survival during L1 diapause.2007

    • Author(s)
      Fukuyama M, Sakuma K, Riyong P, Atsumi Y, Rougvie A, Katada T.
    • Organizer
      16th International Worm Meeting.
    • Place of Presentation
      米国、ロスアンゼルス
    • Year and Date
      20070600
  • [Presentation] Cell signalings mediated through G proteins and drug targets.2007

    • Author(s)
      Katada T.
    • Organizer
      China-Japan Joint Medicine Workshop on Drug Discoveries and Therapeutics 2007
    • Place of Presentation
      中国、済南
    • Year and Date
      20070500
  • [Presentation] ユニークな生化学的性状を示す新奇低分子量gタンパク質群. シンポジウム「細胞内および細胞間ネットワークを制御するgタンパク質シグナルの新展開」2007

    • Author(s)
      堅田利明、紺谷圏二、福山征光、梶保博昭
    • Organizer
      日本薬学会第127年会
    • Place of Presentation
      富山
    • Year and Date
      20070300
  • [Presentation] アティピカルな性状を示す低分子量g蛋白質の機能解析[文部科学省特定領域研究(口頭発表)「細胞情報ネットワークを統合するg蛋白質シグナル研究の新展開」2007

    • Author(s)
      紺谷圏二、堅田利明
    • Organizer
      公開シンポジウム
    • Place of Presentation
      東京
    • Year and Date
      20070100
  • [Presentation] Essential amino acids and carbon sources trigger larval development through the insulin/IGF pathway.2006

    • Author(s)
      Fukuyama M, Rougvie A, Katada T.
    • Organizer
      The 2nd East Asia C. elegans Meeting
    • Place of Presentation
      韓国, ソウル
    • Year and Date
      20061100
  • [Presentation] Novel family of G proteins involving in translation termination and mRNA degradation.2005

    • Author(s)
      Katada T.
    • Organizer
      US-Japan Conference on Drug Development and Rational Drug Design
    • Place of Presentation
      米国、ロスアンゼルス
    • Year and Date
      20050800
  • [Remarks] 研究室ホームページ

    • URL

      http://www.f.u-tokyo.ac.jp/~seiri

  • [Remarks] 特定領域研究ホームページ

    • URL

      http://www.md.tsukuba.ac.jp/basic-med/biochem/kanaholab/Gprotein/g/index.html

URL: 

Published: 2011-06-18   Modified: 2016-04-21  

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