2009 Fiscal Year Final Research Report
Molecular mechanism of a recovery from stalled replication forks coupled with homologous recombination and its regulation
| Project/Area Number |
17080007
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| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | Yokohama City University |
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Principal Investigator |
IWASAKI Hiroshi Yokohama City University, 大学院・生命理工学研究科, 教授 (60232659)
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| Project Period (FY) |
2005 – 2009
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| Keywords | 相同組換え / 複製フォーク / チェックポイント / DNA二重鎖切断 / DNA修復 / リコンビナーゼ / Holliday構造 / 試験管内再構成系 |
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| Research Abstract |
Homologous recombination (HR) plays a crucial role in a recovery from stalled replication forks. Our long-term end is to elucidate molecular mechanism of a coupling reaction and a functional inter-play between DNA replication and HR. For this end, we cut into molecular mechanisms of key reactions for HR such as DNA strand exchange reaction and the Holliday intermediate formation. During the period of this study section, we established an in vitro reconstitution system of Swi5-Sfr1-Rad22-Rad51-dependent strand exchange reaction and Holliday junction formation. One of the most important findings is the 3' to 5' direction of formation and branch migration of Holliday junction.
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[Journal Article] Nbs1 flexibly tethers Ctp1 and Mre11-Rad50 to coordinate DNA double-strand break processing and repair.2009
Author(s)
Williams RS, Dodson GE, Limbo O, Yamada Y, Williams JS, Guenther G, Classen S, Glover JN, Iwasaki H, Russell P, Tainer JA.
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Journal Title
Peer Reviewed
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[Journal Article] A DNA polymeraseαaccessory protein, Mcl1, is required for propagation of centromere structures in fission yeast.2008
Author(s)
Natsume T, Tsutsui Y, Toda T, Dunleavy EM, Pidoux AL, Iwasaki H, Shirahige K, Allshire RC, Yamao F.
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Journal Title
Peer Reviewed
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[Presentation] The fission yeast nip1+ gene, which is involved in DNA double strand break repair togehter with the Mre11-Rad50-Nbs1 complex, is a functional counterpart Saccharomyces Sae2/Com1 and mammalian CtIP.2008
Author(s)
Akamatsu Y, Yasuto Murayama Y, Yamada T, Tsutsui Y, Ohta K, Iwasaki H.
Organizer
DNA Replication and Recombination in Keystone Symposia.
Place of Presentation
Eldorado Hotel & Spa, Santa Fe, New Mexico, USA.
Year and Date
20080210-20080215
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