2009 Fiscal Year Final Research Report
Regulatory mechanism of replication complex formation and relationship between the complex, checkpoints and cell division
| Project/Area Number |
17080008
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| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | National Institute of Genetics |
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Principal Investigator |
ARAKI Hiroyuki National Institute of Genetics, 細胞遺伝研究系, 教授 (20151160)
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| Project Period (FY) |
2005 – 2009
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| Keywords | DNA複製 / 染色体 / 細胞周期 / サイクリン依存性キナーゼ / 真核生物 / 酵母 |
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| Research Abstract |
Chromosome DNA replication is strictly regulated by the cell division cycle, so that chromosome DNA replicates once per cell cycle. This is mainly because the cyclin-dependent protein kinase (CDK), a protein phosphorylating enzyme, regulates the initiation step of chromosome DNA replication. Using budding yeast, we revealed that CDK phosphorylates Sld2 and Sld3 proteins, both phosphorylated proteins bind to the Dpb11 protein and these bindings promote DNA replication. We also found a clue how these bindings function for the initiation of DNA replication.
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