2009 Fiscal Year Final Research Report
Molecular mechanism of microbial DNA partitioning : structure and function
| Project/Area Number |
17080009
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| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | National Institute of Genetics |
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Principal Investigator |
NIKI Hironori National Institute of Genetics, 系統生物研究センター, 教授 (70208122)
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| Co-Investigator(Kenkyū-buntansha) |
TSUTSUI Yasuhiro 国立遺伝学研究所, 分子遺伝研究系, 助教 (00390625)
KIMURA Akatsuki 国立遺伝学研究所, 新分野創造センター, 准教授 (10365447)
FURUYA Kanji 国立遺伝学研究所, 系統生物研究センター, 助教 (90455204)
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| Project Period (FY) |
2005 – 2009
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| Keywords | 染色体 / 分配 / DNA結合 / 無核 / 細胞分裂 |
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| Research Abstract |
Plasmids in bacterial cells have the centromere-based segregation system to ensure their faithful transmission to daughter cells, and is being studied as a model system for chromosome partitioning mechanism. We studied the general mechanism underlie these plasmid partitioning machineries, and found that SopA and ParA proteins do not directly pull of push plasmid DNA, but rather guide the plasmids to the right direction by making the gradient of protein concentrations. Notably, the gradient of SopA or ParA proteins were formed from the polymerizing center within the cell (SopA forms a filament within the cell, and ParA locates on the nuclear). We have been investigating transmission mechanism of bacterial chromosome as well. As the chromosome transmission guidance protein, we identified novel factor MigP and a component of the complex I of electron transport chain NuoG. both are membrane associating proteins and we expect DNA transmission mechanism could associates membrane localization.
DNA checkpoint mechanism in eukaryotic cells stops the cell cycle progression when the cell suffers from DNA damage. We identified the mechanism also affects DNA repair
activities themselves besides its regulate cell cycle progression. We further studied the checkpoint mechanism in dimorphic yeast system. There we found that Chk1 could induce hypha
formation in response to DNA damage, and thus we defined it as the novel cell cycle regulatory function of DNA checkpoint.
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[Journal Article] A DNA polymeraseαaccessory protein, Mcl1, is required for propagation of centromere structures in fission yeast.2008
Author(s)
Natsume T, Tsutsui Y, Sutani T, Dunleavy E, Pidoux A, Iwasaki H, Shirahige K, Allshire R, Yamao F.
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Journal Title
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[Journal Article] Fission yeast Swi5 protein, a novel DNA recombination mediator2008
Author(s)
Haruta, N., Akamatsu, Y., Tsutsui, Y., Kurokawa, Y., Murayama, Y., Arcangioli, B., Iwasaki, H.
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Journal Title
DNA Repair (Amst) 1号7巻
Pages: 1-9
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[Journal Article] Escherichia coli with a linear genome2007
Author(s)
Cu, i T., Moro-oka, N., Ohsumi K., Kodama, K., Ohshima, T., Ogasawara, N., Mori, H., Wanner, B., Niki, H., Horiuchi, T.
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Journal Title
EMBO Rep 2号8巻
Pages: 181-187
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[Journal Article] The Swi5-Sfr1 complex stimulates Rhp51/Rad51- and Dmc1-mediated DNA strand exchange in vitro2006
Author(s)
Haruta, N., Kurokawa, Y., Murayama, Y., Akamatsu, Y., Unzai, S., Tsutsui, Y., Iwasaki, H.
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Journal Title
Nature Struct. Mole. Biol. 9号13巻
Pages: 823-830
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