Transportsome on biomembrane systems: its molecular assembly and physiological function.

2009 Fiscal Year Final Research Report

• Project/Area Number: 17081015
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Osaka University (2007-2010); Kyorin University (2005-2006)
• Principal Investigator: KANAI Yoshikatsu Osaka University, 医学系研究科, 教授 (60204533)
• Co-Investigator(Kenkyū-buntansha): MORI Yasuo 京都大学, 工学研究科, 教授 (80212265) ; TAKESHIMA Hiroshi 京都大学, 薬学研究科, 教授 (70212024) ; SUZUKI Hiroshi 東京大学, 医学部附属病院, 教授 (80206523)
• Project Period (FY): 2005 – 2010
• Keywords: 膜輸送 / トランスポーター / 複合体 / 生体膜 / 上皮輸送

Research Abstract

The purpose of this research is to perform general planning, advertisement, coordination of individual researches, research support and research evaluation to facilitate to promote the researches in the priority area of "Transportsome on biomembrane systems : its molecular assembly and physiological function". Specifically, this research organized and held the conferences of this priority area for the research progress report, public symposia and symposia in academic society' annual meetings. This research also opened a home page for the priority area, issued newsletters, edited review collection and books.

Research Products

• [Journal Article] A novel transporter of SLC22 family specifically transports prostaglandins and co-localizes with 15-hydroxyprostaglandin dehydrogenase in renal proximal tubules. (2010)
  • Author(s): Shiraya K, Hirata T, Hatano R, Nagamori S, Wiriyasermkul P, Jutabha P, Matsubara M, Muto S, Tanaka H, Asano S, Anzai N, Endou H, Yamada A, Sakurai H, Kanai Y.
  • Journal Title: J Biol Chem. 285 Pages: 22141-22151
  • Peer Reviewed
• [Journal Article] TRPM1 is a component of the retinal ON bipolar cell transduction channel in the mGluR6 cascade. (2010)
  • Author(s): Koike C, Obara T, Uriu Y, Numata T, Sanuki R, Miyata K, Koyasu T, Ueno S, Funabiki K, Tani A, Ueda H, Kondo M, Mori Y, Tachibana M, Furukawa T.
  • Journal Title: Proc.Natl.Acad.Sci.USA. 107 Pages: 332-337
  • Peer Reviewed
• [Journal Article] Rim2α determines docking and priming states in insulin granule exocytosis. (2010)
  • Author(s): Yasuda T, Shibasaki T, Minami K, Takahashi H, Mizoguchi A, Uriu Y, Numata T, Mori Y, Miyazaki JI, Miki T, Seino S.
  • Journal Title: Cell Metab. 12 Pages: 117-129
  • Peer Reviewed
• [Journal Article] Common Defects of ABCG2, a High-capacity Urate Exporter, Cause Gout : A Function-based Genetic Analysis in a Japanese Population. (2009)
  • Author(s): Matsuo H, Takada T, Ichida K, Nakamura T, Nakayama A, Ikebuchi Y, Ito K, Kusanagi Y, Chiba T, Tadokoro S, Takada Y, Oikawa Y, Inoue H, Suzuki K, Okada R, Nishiyama J, Domoto H, Watanabe S, Fujita M, Morimoto Y, Naito M, Nishio K, Hishida A, Wakai K, Asai Y, Niwa K, Kamakura K, Nonoyama S, Sakurai Y, Hosoya T, Kanai Y, Suzuki H, Hamajima N, Shinomiya N.
  • Journal Title: Science Trans Med. 1 Pages: 41-48
  • Peer Reviewed
• [Journal Article] Suppression of hippocampal TRPM7 protein prevents delayed neuronal death in brain ischemia. (2009)
  • Author(s): Sun H-S, Jackson MF, Martin LJ, Jansen K, Teves L, Cui H, Kiyonaka S, Mori Y, Jones M, Forder JP, Golde TE, Orser BA, MacDonald JF, Tymianski M.
  • Journal Title: Nature Neurosci. 12 Pages: 1300-1307
  • Peer Reviewed
• [Journal Article] Selective and direct inhibition of TRPC3 channels underlies biological activities of a pyrazole compound. (2009)
  • Author(s): Kiyonaka S, Kato K, Nishida M, Mio K, Numaga T, Sawaguchi Y, Yoshida T, Wakamori M, Mori E, Numata T, Ishii M, Takemoto H, Ojida A, Watanabe K, Uemura A, Kurose H, Morii T, Kobayashi T, Sato Y, Sato C, Hamachi I, Mori Y.
  • Journal Title: Proc.Natl.Acad.Sci.USA. 106 Pages: 5740-5745
  • Peer Reviewed
• [Journal Article] A pathogenic C-terminus-truncated Polycistin-2 mutant enhances receptor-activated Ca2+ entry via association with TRPC3 and TRPC7. (2009)
  • Author(s): Miyagi K, Kiyonaka S, Yamada K, Miki T, Mori E, Kato K, Numata T, Sawaguchi Y, Numaga T, Kimura T, Kanai Y, Kawano M, Wakamori M, Nomura H, Koni I, Yamagishi M, Mori Y.
  • Journal Title: J.Biol.Chem. 284 Pages: 34400-34412
  • Peer Reviewed
• [Journal Article] Receptor for activated C-kinase 1 regulates the cellular localization and function of ABCB4. (2009)
  • Author(s): Ikebuchi Y, Takada T, Ito K, Anzai N, Kanai Y, Suzuki H.
  • Journal Title: Hepatol Res, Hepatol Res. 39 Pages: 1091-1107
  • Peer Reviewed
• [Journal Article] (co-corresponding authors) MG53 nucleates assembly of cell membrane repair machinery. (2009)
  • Author(s): Cai C, Masumiya H, Weisleder N, Matsuda N, Nishi M, Hwang M, Ko J-K, Lin P, Thornton A, Zhao X, Pan Z, Komazaki S, Brotto M, Takeshima H, Ma J.
  • Journal Title: Nature Cell Biol. 11 Pages: 56-64
  • Peer Reviewed
• [Journal Article] A novel role of the C-terminus of b0, +AT in the ER-Golgi trafficking of the rBAT-b0, +AT heterodimeric amino acid transporter. (2009)
  • Author(s): Sakamoto S, Chairoungdua A, Nagamori S, Wiriyasermkul P, Promchan K, Tanaka H, Kimura T, Ueda T, Fujimura M, Shigeta Y, Naya Y, Akakura K, Ito H, Endou H, Ichikawa T, Kanai Y.
  • Journal Title: Biochem J. 417 Pages: 441-448
  • Peer Reviewed
• [Journal Article] TRPM2-mediated Ca2+ influx induces chemokine production in monocytes that aggravates inflammatory neutrophil infiltration. (2008)
  • Author(s): Y amamoto S, Shimizu S, Kiyonaka S, Takahashi N, Wajima T, Hara Y, Negoro T, Hiroi T, Kiuchi Y, Okada T, Kaneko S, Lange I, Fleig A, Penner R, Nishi M, Takeshima H, Mori Y.
  • Journal Title: Nature Med. 14 Pages: 738-747
  • Peer Reviewed
• [Journal Article] Niemann-pick C1-like 1 mediates alpha-tocopherol transport. (2008)
  • Author(s): Narushima K, Takada T, Yamanashi Y, Suzuki H.
  • Journal Title: Mol Pharmacol. 74 Pages: 42-49
  • Peer Reviewed
• [Journal Article] Mutations in glucose transporter 9 gene SLC2A9 cause renal hypouricemia. (2008)
  • Author(s): Matsuo H, Chiba T, Nagamori S, Nakayama A, Domoto H, Phetdee K, Wiriyasermkul P, Kikuchi Y, Oda T, Nishiyama J, Nakamura T, Morimoto Y, Kamakura K, Sakurai Y, Nonoyama S, Kanai Y, Shinomiya N.
  • Journal Title: Am J Hum Genet. 83 Pages: 744-751
  • Peer Reviewed
• [Journal Article] RIM1 confers sustained activity and neurotransmitter vesicle anchoring to presynaptic Ca2+ channels. (2007)
  • Author(s): Kiyonaka S, Wakamori M, Miki T, Uriu Y, Nonaka M, Bito H, Beedle AM, Mori E, Hara Y, De Waard M, Kanagawa M, Itakura M, Takahashi M, Campbell KP, Mori Y.
  • Journal Title: Nature Neurosci. 10 Pages: 691-701
  • Peer Reviewed
• [Journal Article] Fluorine-18-alpha-methyltyrosine positron emission tomography for diagnosis and staging of lung cancer : a clinicopathologic study. (2007)
  • Author(s): Kaira K, Oriuchi N, Otani Y, Shimizu K, Tanaka S, Imai H, Yanagitani N, Sunaga N, Hisada T, Ishizuka T, Dobashi K, Kanai Y, Endou H, Nakajima T, Endo K, Mori M.
  • Journal Title: Clin Cancer Res. 13 Pages: 6369-6378
  • Peer Reviewed
• [Journal Article] TRIC channels are essential for Ca2+ handling in intracellular stores. (2007)
  • Author(s): Yazawa M, Ferrante C, Feng J, Mio K, Ogura T, Zhang M, Lin P-H, Pan Z, Komazaki S, Kato K, Nishi M, Zhao X, Weisleder N, Sato C, Ma J, Takeshima H.
  • Journal Title: Nature. 448 Pages: 78-82
  • Peer Reviewed
• [Journal Article] Calumin, a novel Ca2+-binding transmembrane protein on the endoplasmic reticulum. (2007)
  • Author(s): Zhang M, Yamazaki T, Yazawa M, Treves S, Nishi M, Murai M, Shibata E, Zorzato F, Takeshima H.
  • Journal Title: Cell Calcium. 42 Pages: 83-90
  • Peer Reviewed
• [Journal Article] Junctophilin-mediated channel crosstalk essential for cerebellar synaptic plasticity. (2007)
  • Author(s): Kakizawa S, Kishimoto Y, Hashimoto K, Miyazaki T, Furutani K, Shimizu H, Fukaya M, Nishi M, Sakagami H, Ikeda A, Kondo H, Kano M, Watanabe M, Iino M, Takeshima H.
  • Journal Title: EMBO J. 26 Pages: 1924-1933
  • Peer Reviewed
• [Journal Article] Novel liver-specific organic anion transporter OAT7 that operates the exchange of sulfate conjugates for short chain fatty acid butyrate. (2007)
  • Author(s): Shin HJ, Anzai N, Enomoto A, He X, Kim do K, Endou H, Kanai Y.
  • Journal Title: Hepatology. Pages: 1046-1055
  • Peer Reviewed
• [Journal Article] The PDZ domain protein PDZK1 interacts with human peptide transporter PEPT2 and enhances its transport activity. (2006)
  • Author(s): Noshiro R, Anzai N, Sakata T, Miyazaki H, Terada T, Shin HJ, He X, Miura D, Inui K, Kanai Y, Endou H.
  • Journal Title: Kidney Int. 70 Pages: 275-282
  • Peer Reviewed
• [Journal Article] Nitric oxide activates TRP channels by cysteine S-nitrosylation. (2006)
  • Author(s): Yoshida T, Inoue R, Morii T, Takahashi N, Yamamoto S, Hara Y, Tominaga M, Shimizu S, Sato Y, Mori Y.
  • Journal Title: Nature Chem.Biol. 2 Pages: 596-607
  • Peer Reviewed
• [Journal Article] Coupling of STIM1 to store-operated Ca2+ entry through its constitutive and inducible movement in the endoplasmic reticulum. (2006)
  • Author(s): Baba Y, Hayashi K, Fujii Y, Mizushima A, Watarai H, Wakamori M, Numaga T, Mori Y, Iino M, Hikida M, Kurosaki T.
  • Journal Title: Proc.Natl.Acad.Sci.USA. 103 Pages: 16704-16709
  • Peer Reviewed
• [Journal Article] A novel missense mutation of SLC7A9 frequent in Japanese cystinuria cases affecting the C-terminus of the transporter. (2006)
  • Author(s): Shigeta Y, Kanai Y, Chairoungdua A, Ahmed N, Sakamoto S, Matsuo H, Kim DK, Fujimura M, Anzai N, Mizoguchi K, Ueda T, Akakura K, Ichikawa T, Ito H, Endou H.
  • Journal Title: Kidney Int. 69 Pages: 1198-1206
  • Peer Reviewed
• [Journal Article] Muscle aging is associated with compromised Ca2+ spark signaling and segregated intracellular Ca2+ release. (2006)
  • Author(s): Weisleder N, Brotto M, Komazaki S, Pan Z, Zhao X, Nosek T, Parness J, Takeshima H, Ma J.
  • Journal Title: J.Cell Biol. 174 Pages: 639-645
  • Peer Reviewed
• [Journal Article] Functional uncoupling between Ca2+ release and afterhyperpolarization in mutant hippocampal neurons lacking junctophilins. (2006)
  • Author(s): Moriguchi S, Nishi M, Komazaki S, Sakagami H, Miyazaki T, Masumiya H, Saito S, Watanabe M, Kondo H, Yawo H, Fukunaga K, Takeshima H.
  • Journal Title: Proc.Natl.Acad.Sci.USA. 103 Pages: 10811-10816
  • Peer Reviewed
• [Journal Article] Impaired cerebellar functions in mutant mice lacking DNER. (2006)
  • Author(s): Togho A, Eiraku M, Miyazaki T, Miura E, Kawaguchi S, Nishi M, Watanabe M, Hirano T, Kengaku M, Takeshima H.
  • Journal Title: Mol.Cell.Neurosci. 31 Pages: 326-333
  • Peer Reviewed
• [Journal Article] Modulation of renal apical organic anion transporter 4 function by two PDZ domain-containing proteins. (2005)
  • Author(s): Miyazaki H, Anzai N, Ekaratonawong S, Sakata T, Shin HJ, Jutabha P, Hirata T, Xin H, Nonotuchi H, Tomita K, Kanai Y, Endou H.
  • Journal Title: J.Am.Soc Nephol. 16 Pages: 3498-3506
  • Peer Reviewed
• [Journal Article] Impaired Ca2+ store functions in skeletal and cardiac muscle cells from sarcalumenin-deficient mice. (2005)
  • Author(s): Yoshida M, Minamisawa S, Komazaki S, Shimura M, Kume H, Zhang M, Matsumura K, Nishi M, Saito M, Ishikawa Y, Yanagisawa T, Takeshima H.
  • Journal Title: J.Biol.Chem. 280 Pages: 3500-3506
  • Peer Reviewed
• [Presentation] トランスポーター研究:辿ってきた道とこれから歩む道 (2010)
  • Author(s): 金井好克
  • Organizer: 日本薬学会第130年会
  • Place of Presentation: 岡山
  • Year and Date: 2010-03-29
• [Presentation] 有機溶質トランスポートソーム:その構築と機能的意義 (2010)
  • Author(s): 金井好克
  • Organizer: 特定領域研究公開シンポジウム「生体膜トランスポートソームの分子構築と生理機能」
  • Place of Presentation: 大阪
  • Year and Date: 2010-03-19
• [Presentation] 芳香族アミノ酸誘導体の抗腫瘍効果の確立 (2010)
  • Author(s): 金井好克
  • Organizer: 第11回未来医療交流会
  • Place of Presentation: 大阪
  • Year and Date: 2010-01-12
• [Presentation] Metabolomics reveals in vivo functions of transporters (2009)
  • Author(s): 金井好克
  • Organizer: 24th JSSX Annual Meeting in Kyoto
  • Place of Presentation: 京都
  • Year and Date: 2009-11-29
• [Presentation] メタボロミクスによるトランスポーターの解析 (2009)
  • Author(s): 金井好克, 曽我朋義
  • Organizer: 第82回日本生化学会大会シンポジウム「メタボロミクスが切り拓くヒトシステム生物学」
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-23
• [Presentation] Amino acid transporters in cancer : relevance to its diagnosis and therapeutics (2009)
  • Author(s): 金井好克
  • Organizer: 19th International Congress of Nutrition (ICN) Satellite Symposium "Advanced Knowledges of Amino Acid Research in Human Health and Disease"
  • Place of Presentation: Bangkok, Thailand
  • Year and Date: 2009-10-04
• [Presentation] Metabolomics approaches to orphan transporters (2009)
  • Author(s): 金井好克
  • Organizer: Biomedical Transporters 2009 : Membrane transporters and impact on drug discover
  • Place of Presentation: Thun, Switzerland
  • Year and Date: 2009-08-12
• [Presentation] Novel organic anion transporters and new aspects of organic anion transport in renal proximal tubules (2009)
  • Author(s): 金井好克
  • Organizer: 40th NIPS International Symposium-International Joint Symposium "Physiology of Anion Transport and Cell Volume Regulation (PAT- CVR 2009)"
  • Place of Presentation: 岡崎
  • Year and Date: 2009-08-06
• [Presentation] Transportsomes in Epithelial Function, XXXVIth International Union of Physiological Sciences. (2009)
  • Author(s): 金井好克
  • Organizer: Satellite Symposium "Ion Channels : function, structure, and physiology"
  • Place of Presentation: 京都
  • Year and Date: 2009-08-04
• [Presentation] TRANSPORTSOME IN RENAL ORGANIC SOLUTE TRANSPORT XXXVIth International Union of Physiological Sciences. (2009)
  • Author(s): 金井好克
  • Organizer: Whole day symposium "Epithelial transport : bridges between molecules and function"
  • Place of Presentation: 京都
  • Year and Date: 2009-07-30
• [Presentation] Immuno-proteomic approach to E-C coupling : junctophilin and TRIC channel in cardiac Ca2+ release (2009)
  • Author(s): Takeshima H.
  • Organizer: IUPS Whole-day Symposium.
  • Place of Presentation: Kyoto.
  • Year and Date: 2009-07-28
• [Presentation] アミノ酸トランスポーターと細胞機能 (2009)
  • Author(s): 金井好克
  • Organizer: 第63回日本栄養・食糧学会大会、シンポジウム「アミノ酸の新しい機能とこれからの展開」
  • Place of Presentation: 長崎
  • Year and Date: 2009-05-22
• [Presentation] Functional analysis of ABCB4 and its mutants in vitro (2008)
  • Author(s): Suzuki H, Yoshikado T, Ito K, Takada T.
  • Organizer: 2nd FEBS Special Meeting ABC2008
  • Place of Presentation: Innsbruck, Austria.
  • Year and Date: 20080301-20080308
• [Presentation] Ca2+チャネルシグナルソームの分子構成と生理機能 (2008)
  • Author(s): 森泰生
  • Organizer: 特定領域研究「生体超分子構造」第5回公開シンポジウム
  • Place of Presentation: つくば市
  • Year and Date: 2008-12-19
• [Presentation] 特定領域トランスポートソーム (2006)
  • Author(s): 金井好克
  • Organizer: 第1回トランスポーター研究会
  • Place of Presentation: 東京 特別講演
  • Year and Date: 2006-12-16
• [Presentation] Ryanodine receptor-mediated Ca2+ release from muscle sarcoplasmic reticulum (2006)
  • Author(s): Takeshima H.
  • Organizer: Gordon Research Conference ; Muscle Excitation-Contraction Coupling.
  • Place of Presentation: New London, USA.
  • Year and Date: 2006-06-09
• [Book] Transient Receptor Potential Channels, Receptor Signaling Integration by TRP Channelsomes (2011)
  • Author(s): Mori Y, Kajimoto T, Nakao A, Takahashi N, Kiyonaka S.
  • Total Pages: 373-389
  • Publisher: Springer
• [Book] 遺伝子医学MOOK 最新トランスポーター研究(編集、分担執筆)12号 (2009)
  • Author(s): 金井好克
  • Total Pages: 29-34,68-75
• [Book] トランスポーター科学最前線(アミノ酸トランスポーター:過去、現在、そして未来へ)(分担執筆) (2008)
  • Author(s): 金井好克
  • Total Pages: 223-244
  • Publisher: 京都廣川書店
• [Remarks] ホームページ等
  • URL: http://www.med.osaka-u.ac.jp/pub/pharma1/transportsome/top.html