2009 Fiscal Year Final Research Report
The role of CRMP family proteins in the establishment of neural tissue architectures
| Project/Area Number |
17082006
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| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | Yokohama City University |
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Principal Investigator |
GOSHIMA Yoshio Yokohama City University, 医学研究科, 教授 (00153750)
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| Co-Investigator(Kenkyū-buntansha) |
OOSHIMA Toshio 早稲田大学, 理工学術院, 教授 (20311334)
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| Project Period (FY) |
2005 – 2009
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| Keywords | CRMP / セマフォリン / プレキシン / 神経ガイダンス / 組織構築 |
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| Research Abstract |
In developing brain, axon and dendritic guidance are regulated by repulsive and attractive axon guidance molecules such as semaphorin3A (Sema3A) and netrin. Collpapsin response mediator protein (CRMP) has originally been identified as an intracellular protein that mediates Sema3A. We found that Sema3A elicits axoplasmic transport that may be involved in regulating the localization of AMPA type glutamate receptors in hippocampal neurons. To elucidate in vivo role of CRMPs, we generated several crmp1 and other crmp family gene-deficient mice and performed phenotypic analysis of these mice. For instance, in crmp1-deficient mice, the cell migration of cortical neurons at early embryonic stages is retarded. CRMP1 is colocalized with disabled-1 (Dab1), an adaptor protein in Reln signaling. In the Relnrl/rl cortex, CRMP1 and Dab1 are expressed at a higher level, yet tyrosine phosphorylated at a lower level. Loss of crmp1 in a dab1 heterozygous background lead to the disruption of hippocampal lamination, a Reeler-like phenotype. CRMP1 is also involved in Sema3A-induced localization of AMPA receptors and spine development in the cerebral cortex. In the cultured cortical neurons from crmp1 mice, Sema3A increases the density of clusters of synapsin I and postsynaptic density-95, but this increase is markedly attenuated in crmp1-deficient mice. In our study of C. elegans, we identified several mutant alleles which show aberrant localization of netrin/UNC-6 and axon guidance defects. We therefore conclude that the regulation of the glutamate receptor and the axon guidance molecule localization may play an important role in a wide variety of developmental processes from cell migration to neural network formation.
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[Journal Article] Semaphorin3A alleviates skin lesions and scratching behavior in NC/Nga mice, an atopic dermatitis model.2008
Author(s)
Yamaguchi J, Nakamura F, Aihara M, Yamashita N, Usui H, Hida T, Takei K, Nagashima Y, Ikezawa Z, Goshima Y.
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Journal Title
J Invest Dermatol 128
Pages: 2842-2849
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[Journal Article] A selective Sema3A inhibitor enhances regenerative responses and functional recovery of the injured spinal cord.2007
Author(s)
Kaneko S, Iwanami A, Nakamura M, Kishino A, Kikuchi K, Shibata S, Okano HJ, Ikegami T, Moriya A, Konishi O, Nakayama C, Kumagai K, Kimura T, Sato Y, Goshima Y, Taniguchi M, Ito M, He Z, Toyama Y, Okano H.
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Journal Title
Nat Med 12
Pages: 1380-1389
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[Journal Article] Regulation of spine development by semaphorin3A through cyclin-dependent kinase 5 phosphorylation of collapsin response mediator protein 1.2007
Author(s)
Yamashita N, Morita A, Uchida Y, Nakamura F, Usui H, Ohshima T, Taniguchi M, Honnorat J, Thomasset N, Takei K, Takahashi T, Kolattukudy P, Goshima Y.
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Journal Title
J Neuroscience 27
Pages: 12546-12554
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[Journal Article] Collapsin Response Mediator Protein 1 Mediates Reelin Signaling in Cortical Neuronal Migration.2006
Author(s)
Yamashita N, Uchida Y, Ohshima T, Hirai S, Nakamura F, Taniguchi M, Mikoshiba K, Honnorat J, Kolattukudy P, Thomasset N, Takei K, Takahashi T, Goshima Y.
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Journal Title
J. Neurosci. 26
Pages: 13357-13362
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[Journal Article] Transient Alterations in granule cell proliferation, apoptosis and migration in post-natal developing cerebellum of CRMP1-/- mice.2006
Author(s)
Charrier E, Mosinger B, Meissirel C, AgueraM, Rogemond V, Reibel S, Salin P, Chounlamountri N, Perrot V, Belin MF, Goshima Y, Honnorat J, Thomasset N, Kolattukudy P
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Journal Title
Genes to Cells 11
Pages: 1337-1352
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[Journal Article] A novel mouse FK-506 binding protein homolog alters growth cone morphology.2006
Author(s)
Nakajima O, Nakamura F, Yamashita N, Tomita Y, Saito F, Okada T, Iwamatsu A, Kondo E, Fujisawa H, Takei K, Goshima Y
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Journal Title
Biochem Biophys. Res. Comm. 346
Pages: 140-149
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[Presentation] CRMP1 regulates spine maturation through mediating Semaphorin3A signaling in vivo.2007
Author(s)
Yamashita N, Morita A, Uchida Y, Ohshima T, Nakamura F, Usui H, Honnorat J, Kolattukudy P, Thomasset N, Goshima Y
Organizer
Annual Meeting of the Society for Neuroscience
Place of Presentation
San Diego, USA
Year and Date
20071100
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[Presentation] an actin-binding protein, participates in the Semaphorin3A signaling through the interaction with collapsing response mediator proteins2006
Author(s)
Kumeta K, Nakayama Y, Kuramata E, Yamane M, Yamashita N, Uchida Y, Gengyo-Ando K, Mitani S, Ogura K, Nakamura F, Goshima Y, Filamin A
Organizer
52th Japan Neurochemical Society Meeting
Place of Presentation
伊香保
Year and Date
2006-06-22
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