Basement membrane regulation in C.elegans organogenesis

2009 Fiscal Year Final Research Report

• Project/Area Number: 17082011
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Kwansei Gakuin University (2008-2009); The Institute of Physical and Chemical Research (2005-2007)
• Principal Investigator: NISHIWAKI Kiyoji Kwansei Gakuin University, 理工学部, 教授 (30342827)
• Co-Investigator(Kenkyū-buntansha): KUBOTA Yukihiko 関西学院大学, 理工学部, 研究員 (70333325) ; OHKURA Kiyotaka 独立行政法人理化学研究所, 細胞移動研究チーム, 研究員 (30415103) ; IHARA Shinji 独立行政法人理化学研究所, 細胞移動研究チーム, 研究員 (70373272)
• Project Period (FY): 2005 – 2009
• Keywords: 線虫 / ADAMTS family / MIG-17 / 抑圧遺伝子 / fibulin / nidogen / type IV collagen

Research Abstract

An ADAMTS family protease MIG-17 is recruited to the gonadal basement membrane in a prodomain-dependent manner, where it is activated to control gonadal leader cell migration in Caenorhabditis elegans. We found that fibulin-1 and type IV collagen act downstream of MIG-17 to recruit nidogen and that type IV collagen also activates a nidogen-independent pathway for directional control of leader cell migration.

Research Products

• [Journal Article] C. elegans mig-6 encodes papilin isoforms that affect distinct aspects of DTC migration, and interacts genetically with mig-17 and collagen IV. (2009)
  • Author(s): Kawano, T., Zheng, H., Merz, D.C., Kohara, Y., Tamai, K.K., Nishiwaki, K., Culotti, J.G.
  • Journal Title: Development 136 Pages: 1433-1442
  • Peer Reviewed
• [Journal Article] MIG-17/ADAMTS controls cell migration by recruiting nidogen to the basement membrane in C. (2008)
  • Author(s): Kubota, Y., Ohkura, K., Tamai, K.K., Nagata, K., Nishiwaki, K.
  • Journal Title: elegans. Proc. Natl. Acad. Sci. USA 105 Pages: 20804-20809
  • Peer Reviewed
• [Journal Article] Stage-specific activation of MIG-17/ADAMTS controls cell migration in Caenorhabditis elegans (2008)
  • Author(s): Ihara, S., Nishiwaki, K.
  • Journal Title: FEBS J. 275 Pages: 4296-4305
  • Peer Reviewed
• [Journal Article] bHLH transcription factors regulate organ morphogenesis via activation of an ADAMTS protease in C. (2007)
  • Author(s): Tamai, K.K., Nishiwaki, K.
  • Journal Title: elegans. Dev. Biol. 308 Pages: 562-571
  • Peer Reviewed
• [Journal Article] Prodomain-dependent tissue targeting of an ADAMTS protease controls cell migration in Caenorhabditis elegans. (2007)
  • Author(s): Ihara, S., Nishiwaki, K.
  • Journal Title: EMBO J. 26 Pages: 2607-2620
  • Peer Reviewed
• [Journal Article] MRG-1, an autosome-associated protein, silences X-linked genes and protects germline immortality in C. elegans. (2007)
  • Author(s): Takasaki, T., Liu, Z., Habara, Y., Nishiwaki, K., Nakayama, J., Inoue, K., Sakamoto, H., Strome, S.
  • Journal Title: Development 134 Pages: 757-767
  • Peer Reviewed
• [Journal Article] プロドメインがADAMTSプロテアーゼの組織局在に必須である (2007)
  • Author(s): 伊原伸治、西脇清二
  • Journal Title: 実験医学 25 Pages: 2891-2894
• [Journal Article] 分泌型ADAMTSプロテアーゼによる器官形成の制御 (2007)
  • Author(s): 久保田幸彦、西脇清二
  • Journal Title: 細胞工学 26 Pages: 1136-1141
• [Journal Article] 特集「細胞外環境による形態形成の制御-新しい生物学の胎動」 (2007)
  • Author(s): 西脇清二
  • Journal Title: 細胞工学 26,10月号 Pages: 1110-1112
• [Journal Article] Chondroitin acts in the guidance of gonadal distal tip cells in C. elegans. (2006)
  • Author(s): Suzuki, N., Toyoda, H., Sano, M., Nishiwaki, K.
  • Journal Title: Dev. Biol. 300 Pages: 635-646
  • Peer Reviewed
• [Journal Article] Behavioral genetics of Caenorhabditis elegans unc-103-encoded erg-like K(+) channel. (2006)
  • Author(s): Reiner, D.J., Weinshenker, D., Tian, H., Thomas, J.H., Nishiwaki, K., Miwa, J., Gruninger, T., Leboeuf, B., Garcia, L.R.
  • Journal Title: J. Neurogenet 20 Pages: 41-66
  • Peer Reviewed
• [Journal Article] Hormonal control of C. elegans dauer formation and life span by a Rieske-like oxygenase. (2006)
  • Author(s): Rottiers, V., Motola, D.L., Gerisch, B., Cummins, C.L., Nishiwaki, K., Mangelsdorf, D.J., Antebi A.
  • Journal Title: Dev. Cell 10 Pages: 1-10
  • Peer Reviewed
• [Journal Article] The conserved oligomeric Golgi complex acts in organ morphogenesis via glycosylation of an ADAM protease in C. elegans. (2006)
  • Author(s): Kubota, Y., Sano, M., Goda, S., Suzuki, N., Nishiwaki, K.
  • Journal Title: Development 133 Pages: 263-273
  • Peer Reviewed
• [Journal Article] C. elegans as a model system to study the function of the COG complex in animal development. (2006)
  • Author(s): Kubota, Y., Nishiwaki, K.
  • Journal Title: Biol. Chem. 387 Pages: 1031-1035
  • Peer Reviewed
• [Journal Article] SRC-1, a non-receptor type of protein tyrosine kinase, controls the direction of cell and growth cone migration in C. elegans. (2005)
  • Author(s): Itoh, B., Hirose, T., Takata, N., Nishiwaki, K., Koga, M., Ohshima, Y., Okada, M.
  • Journal Title: Development 132 Pages: 5161-5172
  • Peer Reviewed
• [Presentation] Genetic suppressors of defective gonadogenesis in gon-1 (ADAMTS Metalloprotease) mutants in C. elegans. (2009)
  • Author(s): Masaki Kakehi, Yukihiko Kubota, Kiyoji Nishiwaki
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-09
• [Presentation] Fibulin-1 interacts with type IV collagen to regulate GON-1/ADAMTS activity and DBL-1/BMP signaling in C. elegans. (2009)
  • Author(s): Yukihiko Kubota, Kayo Nagata, Kiyoji Nishiwaki
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-09
• [Presentation] C. elegansコンドロイチン重合因子MIG-22の優性変異による. MIG-17/ADAMTS欠損の抑圧に関与する分子の同定 (2009)
  • Author(s): 佐々壽浩、豊田英尚、大蔵清貴、西脇清二
  • Organizer: BMB2008
  • Place of Presentation: 神戸
  • Year and Date: 2009-12-09
• [Presentation] C. elegansのMIG-17/ADAMTSはnidogenを基底膜にリクルートすることにって細胞移動を制御する (2009)
  • Author(s): 久保田幸彦、大蔵清貴、玉井克幸、長田香代、西脇清二
  • Organizer: BMB2008
  • Place of Presentation: 神戸
  • Year and Date: 2009-12-09
• [Presentation] Fibulin-1 interacts with type IV collagen to regulate GON-1/ADAMTS activity and DBL-1/BMP signaling in C. elegans. (2009)
  • Author(s): Yukihiko Kubota, Kiyoji Nishiwaki
  • Organizer: Gordon Research Conference on Matrixmetalloproteinases
  • Place of Presentation: Les Diablerets, Switzerland
  • Year and Date: 2009-09-03
• [Presentation] MIG-17/ADAMTS controls cell migration by recruiting nidogen to the basement membrane in C. elegans. (2008)
  • Author(s): Kubota, Y., Ohkura, K., Nagata, K., Tamai, K.K., Nishiwaki, K.
  • Organizer: American Society for Matrix Biology
  • Place of Presentation: San Diego, USA
  • Year and Date: 2008-12-07
• [Presentation] Suppression of mig-17 DTC migration defects by a dominant mutation in mig-22. (2008)
  • Author(s): Toshihiro Sassa, Hidenao Toyoda, Kiyotaka Ohkura, Kiyoji Nishiwaki.
  • Organizer: East Asia C. elegans Meeting
  • Place of Presentation: Shanghai, China
  • Year and Date: 2008-04-18
• [Presentation] 発生段階に依存したC. elegans ADAMTSプロテアーゼMIG活性化 (2007)
  • Author(s): 伊原伸治西脇清二
  • Organizer: 第30回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-11
• [Presentation] Molecular cascade downstream of MIG-17/ADAMTS controlling organ morphogenesis in C. elegans. (2007)
  • Author(s): Yukihiko Kubota, Kiyotaka Ohkura, Kayo Nagata, Kiyoji Nishiwaki
  • Organizer: 第30回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-11
• [Presentation] A dominant mutation of mig-22 suppresses DTC migration defects of mig-17. (2007)
  • Author(s): Sassa, T., Nishiwaki, K.
  • Organizer: 16th International C. elegans Meeting
  • Place of Presentation: Los Angels, USA
  • Year and Date: 2007-06-27
• [Presentation] Molecular cascade downstream of MIG-17/ADAMTS controlling cell migration in C. elegans. (2007)
  • Author(s): Kubota, Y., Ohkura, K., Nagata, K., Nishiwaki K.
  • Organizer: 16th International C. elegans Meeting
  • Place of Presentation: Los Angels, USA
  • Year and Date: 2007-06-27
• [Presentation] Molecular cascade downstream of MIG-17/ADAMTS controlling cell migration in C. elegans. (2007)
  • Author(s): Nishiwaki, K.
  • Organizer: Gordon Research Conference on Matrix Metalloproteinases
  • Place of Presentation: Il Ciocco, Italy
  • Year and Date: 2007-06-03
• [Presentation] Prodomain-dependent tissue targeting and activation of MIG-17, an ADAMTS protease controlling cell migration in Caenorhabditis elegans. (2007)
  • Author(s): Ihara, S., Nishiwaki, K.
  • Organizer: Gordon Research Conference on Matrix Metalloproteinases
  • Place of Presentation: Il Ciocco, Italy
  • Year and Date: 2007-06-03
• [Presentation] C. elegansのADAMTSプロテアーゼMIG-17による器官形成の制御 (2007)
  • Author(s): 久保田幸彦、大蔵清貴、長田香代、西脇清二
  • Organizer: 第40回日本発生生物学会・第59回日本細胞生物学会合同大会
  • Place of Presentation: 福岡
  • Year and Date: 2007-05-27
• [Presentation] C. elegansの器官形成における基底膜ADAMTSプロテアーゼMIG-17の役割 (2006)
  • Author(s): 西脇清二
  • Organizer: 分子生物学会2006 forum
  • Place of Presentation: 名古屋
  • Year and Date: 2006-12-06
• [Presentation] Molecular dissection of MIG-17, an ADAMTS family protease that controls cell migration in C. elegans. (2006)
  • Author(s): Shinji Ihara, Kiyoji Nishiwaki
  • Organizer: East Asia C. elegans Meeting
  • Place of Presentation: Seoul, Korea
  • Year and Date: 2006-11-15
• [Presentation] fibulin-1 dominant mutations suppress cell migration defects of mig-17 mutants in a nidogen-1-dependent manner in C. elegans. (2006)
  • Author(s): Yukihiko Kubota, Kayo Nagata, Kiyoji Nishiwaki
  • Organizer: East Asia C. elegans Meeting
  • Place of Presentation: Seoul, Korea
  • Year and Date: 2006-11-15
• [Presentation] C. elegans fibulin-1の優性変異はnidogen-1に依存してmig-17変異体の細胞移動異常を抑圧する (2005)
  • Author(s): 久保田幸彦, 黒木理恵, 西脇清二
  • Organizer: 第28回日本分子生物学会年会
  • Place of Presentation: 福岡
  • Year and Date: 2005-12-07
• [Presentation] Role of MIG-17 ADAM protease that controls organ morphogenesis in C. elegans. (2005)
  • Author(s): Nishiwaki, K.
  • Organizer: 第78回日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2005-10-19
• [Presentation] THE COG COMPLEX ACTS IN ORGAN MORPHOGENESIS VIA GLYCOSYLATION OF AN ADAM PROTEASE IN C. ELEGANS. (2005)
  • Author(s): Nishiwaki, K.
  • Organizer: 4th General Meeting of International Proteolysis Society
  • Place of Presentation: Quebec City, Canada
  • Year and Date: 2005-10-15
• [Presentation] ADAMs in migration of distal tip cells in C. elegans. (2005)
  • Author(s): Nishiwaki, K.
  • Organizer: The Gordon Research Conference on Matrix Metalloproteinases
  • Place of Presentation: Montana, USA
  • Year and Date: 2005-08-28
• [Presentation] FBL-1 interacts with ADAM proteases and NID-1 to control cell migration in C. elegans. (2005)
  • Author(s): Yukihiko Kubota, Rie Kuroki, Kiyoji Nishiwaki.
  • Organizer: 15th International C. elegans Meeting
  • Place of Presentation: Los Angels, USA
  • Year and Date: 2005-06-26
• [Presentation] Specific mutations in type IV collagen suppress the cell migration defects in mig-17 mutants. (2005)
  • Author(s): Kiyotaka Ohkura, Kiyoji Nishiwaki
  • Organizer: 15th International C. elegans Meeting
  • Place of Presentation: Los Angels, USA
  • Year and Date: 2005-06-26
• [Presentation] Glycosylation of MIG-17 ADAM proteaseessential for controlling cell migration in C. elegans. (2005)
  • Author(s): Ihara, S., Nishiwaki, K.
  • Organizer: 15th International C. elegans Meeting
  • Place of Presentation: Los Angels, USA
  • Year and Date: 2005-06-26
• [Presentation] 線虫C. elegansの器官形成における分泌型ADAMプロテアーゼMIG-17の役割 (2005)
  • Author(s): 西脇清二
  • Organizer: 第37回日本結合組織学会学術大会
  • Place of Presentation: 富山
  • Year and Date: 2005-05-26
• [Book] Principles of Developmental Genetics(Sally A. Moody, ed. ) (2007)
  • Author(s): Nishiwaki, K., Kubota, Y
  • Total Pages: 404-423
  • Publisher: Academic Press