2009 Fiscal Year Final Research Report
Migration, disposition of cortical interneurons and establishment of area specificity
| Project/Area Number |
17100005
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| Research Category |
Grant-in-Aid for Scientific Research (S)
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| Allocation Type | Single-year Grants |
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| Research Field |
Neuroscience in general
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| Research Institution | Osaka University |
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Principal Investigator |
MURAKAMI Fujio Osaka University, 大学院・生命機能研究科, 教授 (20089882)
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| Co-Investigator(Kenkyū-buntansha) |
TANABE Yasuto 大阪大学, 大学院・生命機能研究科, 准教授 (10311309)
KOBAYASHI Hiroaki 大阪大学, 大学院・生命機能研究科, 助教 (20314396)
INAMURA Naoko 大阪大学, 大学院・生命機能研究科, 特任研究員 (20397623)
TANAKA Daisuke 慶應義塾大学, 医学系研究科, 特別研究助教 (90456921)
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| Project Period (FY) |
2005 – 2009
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| Keywords | GABA / GFP / トレーサー / 介在ニューロン / 大脳基底核原基 / 大脳皮質 / 細胞移動 / 領野特異性 |
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| Research Abstract |
Migrating neurons are thought to travel from their origin near the ventricle to distant territories along tereotypical pathways by detecting environmental cues in the extracellular milieu. Here, we report a novel mode of neuronal migration that challenges this view. We performed long-term, time-lapse imaging of medial ganglionic eminence (MGE)-derived cortical interneurons tangentially migrating in the marginal zone MZ) in flat-mount cortices. We find that they exhibit a diverse range of behaviors in terms of the rate and direction of migration. Curiously, a predominant population of these neurons repeatedly changes its direction of migration in an unpredictable manner. Trajectories of migration vary from one neuron to another. The migration of individual cells lasts for long periods, sometimes up to 2 d. Theoretical analyses reveal that these behaviors can be modeled by a random walk. Furthermore, MZ cells migrate from the cortical subventricular zone to the cortical plate, transiently accumulating in the MZ. These results suggest that MGE-derived cortical interneurons, once arriving at the MZ, are released from regulation by guidance cues and initiate random walk movement, which potentially contributes to their dispersion throughout the cortex. They also uggest that the final position individual interneurons are determined as a consequence of a stochastic process.
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Research Products
(24 results)
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[Journal Article] Crucial roles of Robo proteins in midline crossing of cerebellofugal axons and lack of their up-regulation after midline crossing.2008
Author(s)
Tamada A, Kumada T, Zhu Y, Matsumoto T, Hatanaka Y, Muguruma K, Chen Z, Tanabe Y,Torigoe M, Yamauchi K, Oyama H, Nishida K, Murakami F.
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Journal Title
Neural Dev. Nov 5
Pages: 3-29.
Peer Reviewed
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[Journal Article] The role of Slit-Robo signaling in the generation, migration and morphological differentiation of cortical interneurons.2008
Author(s)
Andrews W, Barber M, Hernadez-Miranda LR, Xian J, Rakic S, Sundaresan V, Rabbitts TH, Pannell R, Rabbitts P, Thompson H, Erskine L, Murakami F, Parnavelas JG.
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Journal Title
Dev Biol. 313(2)
Pages: 6648-6058.
Peer Reviewed
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