2009 Fiscal Year Final Research Report
Deep-sea deployment of a Microfabricated In Situ Gene Analysis System and Its Functional Sophistication
| Project/Area Number |
17106012
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| Research Category |
Grant-in-Aid for Scientific Research (S)
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| Allocation Type | Single-year Grants |
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| Research Field |
Naval and maritime engineering
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| Research Institution | The University of Tokyo |
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Principal Investigator |
FUJII Teruo The University of Tokyo, 生産技術研究所, 教授 (30251474)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAMOTO Takatoki 東京大学, 生産技術研究所, 助教 (20322688)
FUKUBA Tatsuhiro 東京大学, 生産技術研究所, 特任准教授 (80401272)
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| Project Period (FY) |
2005 – 2009
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| Keywords | マイクロ流体デバイス / 現場分析装置 / 遺伝子解析 / 深海 / PCR / DNA精製 / 微生物 / ATP |
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| Research Abstract |
In this study, we developed an in situ gene analysis system that can be applied to oceanography. The system was successfully deployed in deep-sea environments to analyze microbial communities. By utilizing microfluidic devices, sample pre-treatment function was integrated. In situ microbial activity assay method was also established in this study. Corroborative work was carried out with Monterey Bay Aquarium Research Institute (MBARI) and it resulted in a successful evaluation of the system for red-tide plankton monitoring.
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[Journal Article] ガス漏洩モニタリング (間接検出法 : バイオマーカー利用モニタリング)―遺伝子マーカーによるメタン漏洩の原位置検出技術の開発―2008
Author(s)
帆秋利洋, 沖田紀子, 布施博之, 福場辰洋, 藤井輝夫, 鋤崎俊二, 天石文, 片山美津瑠, 吉田光毅, 安田博和, 藤原靖
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Journal Title
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[Presentation] Development of Integrated in situ Analyzers (IISA) for Oceanography Applications2007
Author(s)
T. Fukuba, A. Miyaji, N. Fukuzawa, C. Provin, T. Yamamoto, L. Glutz, T. Okamoto, T. Fujii
Organizer
μTAS 2007, Proceedings, (844-846)
Place of Presentation
Paris, France
Year and Date
2007-10-09
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[Presentation] Development of miniaturized in situ analysis devices for biological and chemical oceanography2005
Author(s)
T. Fukuba, A. Imhof, M. Matsunaga, N. Takagi, T. Yamamoto, K. Okamura, T. Naganuma, T. Fujii
Organizer
Microtechnology in Medicine and Biology, 2005. 3rd IEEE/EMBS Special Topic Conference, Proceedings, (56-54)
Place of Presentation
Hawaii, USA
Year and Date
20050512-20050515
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[Presentation] 深海熱水地帯における化学及び生物活動に関する現場複合計測の試み2005
Author(s)
福場辰洋, 岡本拓士, 長沼毅, 金子亮, 林徹, 許正憲, 吉田尊雄, 岡村慶, 下島公紀, 前田義明, 小池祐一, 藤井輝夫
Organizer
2005年度日本地球化学会第52回年会講演会要旨集 (7)
Place of Presentation
沖縄
Year and Date
2005-09-26
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