Promotion of cardiovascular physiome focusing on coronary microcirculation and myocardial crossbridge dynamics

2008 Fiscal Year Final Research Report

• Project/Area Number: 17200033
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biomedical engineering/Biological material science
• Research Institution: Kawasaki University of Medical Welfare (2007-2008); Kawasaki College of Allied Health Professions (2005-2006)
• Principal Investigator: KAJIYA Fumihiko Kawasaki University of Medical Welfare, 医療技術学部, 教授 (70029114)
• Co-Investigator(Kenkyū-buntansha): HIRAMATSU Osamu 川崎医療福祉大学, 医療技術学部, 准教授 (50208849) ; TACHIBANA Hiroyuki 川崎医療福祉大学, 医療技術学部, 講師 (00241216) ; MOCHIZUKI Seiichi 川崎医療福祉大学, 医療技術学部, 教授 (60259596) ; KATAOKA Noriyuki 川崎医療福祉大学, 医療技術学部, 准教授 (20250681) ; GOTO Masami 川崎医療福祉大学, 医療技術学部, 教授 (50148699)
• Co-Investigator(Renkei-kenkyūsha): YAGI Naoto (財)高輝度科学研究センター, 利用研究促進部門II, 主席研究員 (80133940) ; SUGIURA Seiryo 東京大学, 大学院新領域創成科学研究科, 教授 (10272551) ; HIMENO Ryutaro 理化学研究所, 次世代スーパーコンピュータ開発実施本部, グループディレクター (60342838) ; SHIMIZU Juichiro 奈良県立医科大学, 医学部, 准教授 (80294403) ; MOHRI Satoshi 岡山大学, 大学院・医歯薬学総合研究科, 講師 (00294413) ; NAKAMURA Kazufumi 岡山大学, 大学院・医歯薬学総合研究科, 助教 (10335630) ; OGASAWARA Yasuo 川崎医科大学, 医学部, 准教授 (10152365) ; SUGA Hiroyuki 国立循環器病センター研究所, 所長 (90014117) ; NINOMIYA Yoshifumi 岡山大学, 大学院医歯薬学総合研究科, 教授 (70126241) ; TSUJIOKA Katsuhiko 川崎医科大学, 医学部, 教授 (30163801) ; KOBAYASHI Naoya 岡山大学, 医学部・歯学部付属病院, 講師 (10325102)
• Project Period (FY): 2005 – 2008
• Keywords: フィジオーム / 冠微小循環 / SPring-8 / アクチン・ミオシン架橋

Research Abstract

心臓が統合的に機能を発揮するためには、アクチン・ミオシン(AM)架橋の形成と解離と冠微小循環の動態が正常に機能する必要がある。そこで、AM挙動はSPring-8放射光を用いて摘出灌流心(ラット)のX線回折像によって解析し、心筋微小循環は高倍率/高速CCDビデオ顕微鏡で可視化観測した。その結果、AM架橋と微小血管の相互作用が心機能と流れの維持にとって重要であることが示された。これらの結果をフィジオミックに評価するため心筋と冠血管のクロストークを考慮した冠循環のモデルを作成し、モデル論的解析を行った。

Research Products

• [Journal Article] Mode of frequency distribution of external work efficiency of arrhythmic beats during atrial fibrillation remains normal in canine heart (2009)
  • Author(s): Shimizu J, Mohri S, Ito H, Takaki M, Suga H.
  • Journal Title: J Physiol Sci. 59(1) Pages: 75-80
  • Peer Reviewed
• [Journal Article] Intracellular Ca^<2+> Responses in Cultured Endothelial Cells to Mechanical Stimulation by Laser Tweezers (2008)
  • Author(s): N Kataoka, K Hashimoto, S Kudo, R Yamaguchi, K Tsujioka, F Kajiya
  • Journal Title: Journal of Biomechanical Science and Engineering 3(2) Pages: 116-123
  • Peer Reviewed
• [Journal Article] Direct Measurement of NO in Biological Samples (2008)
  • Author(s): S Mochizuki, M Goto, Y Ogasawara, F Kajiya
  • Journal Title: Key Engineering Materials 381-382 Pages: 343-344
  • Peer Reviewed
• [Journal Article] Application of a flow-through type pH/CO_2 sensor system based on ISFET for evaluation of the glucose dependency of the metabolic pathways in cultured cells (2008)
  • Author(s): S Mohri, A Yamada, N Goda, M Nakamura, K Naruse, F Kajiya
  • Journal Title: Sensors and Acyuators B: Chemical 134 Pages: 447-450
  • Peer Reviewed
• [Journal Article] Altered nano/micro-order elasticity of pulmonary artery smooth muscle cells of patients with idiopathic pulmonary arterial hypertension (2008)
  • Author(s): K Nakamura, J Shimizu, N Kataoka, K Hashimoto, T Ikeda, H Fujio, K Ohta-Ogo, A Ogawa, A Miura, S Mohri, S Nagase, H Morita, KF Kusano, H Date, H Matsubara, S Mochizuki, K Hashimoto, F Kajiya, T Ohe
  • Journal Title: Int J Cardiol. Epub.
  • Peer Reviewed
• [Journal Article] Direct measurement of nitric oxide concentration in CAPD dialysate (2008)
  • Author(s): S Mochizuki, A Takayama, T Sasaki, H Horike, N Kashihara, Y Ogasawara, F Kajiya, T Kitawaki, H Oka
  • Journal Title: Perit. Dial. Int. 29(1) Pages: 111-114
  • Peer Reviewed
• [Journal Article] Responses of single-ventricular myocytes to dynamic axial stretching (2008)
  • Author(s): Nishimura S, Seo K, Nagasaki M, Hosoya Y, Yamashita H, Fujita H, Nagai R, Sugiura S
  • Journal Title: Prog Biophys Mol Biol. 97(2-3)(Review) Pages: 282-97
  • Peer Reviewed
• [Journal Article] Development of high-resolution 4D in vivo-CT for visualization of cardiac and respiratory deformations of small animals (2008)
  • Author(s): Sera T, Yokota H, Fujisaki K, Fukasaku K, Tachibana H, Uesugi K, Yagi N, Himeno R
  • Journal Title: Phys Med Biol. 53(16) Pages: 4285-301
  • Peer Reviewed
• [Journal Article] The looped heart does not save energy by maintaining the momentum of blood flowing in the ventricle (2008)
  • Author(s): Watanabe H, Sugiura S, Hisada T
  • Journal Title: Am J Physiol Heart Circ Physiol. 294(5) Pages: H2191-6
  • Peer Reviewed
• [Journal Article] In vivo imaging in mice reveals local cell dynamics and inflammation in obese adipose tissue (2008)
  • Author(s): Nishimura S, Manabe I, Nagasaki M, Seo K, Yamashita H, Hosoya Y, Ohsugi M, Tobe K, Kadowaki T, Nagai R, Sugiura S
  • Journal Title: J Clin Invest. 118(2) Pages: 710-21
  • Peer Reviewed
• [Journal Article] Role of Cu,Zn-SOD in the Synthesis of Endogenous Vasodilator Hydrogen Peroxide during Reactive Hyperemia in Mouse Mesenteric Microcirculation in Vivo (2008)
  • Author(s): Yada T, Shimokawa H, Morikawa K, Takaki A, Shinozaki Y, Mori H, Goto M, Ogasawara Y, Kajiya F
  • Journal Title: Am J Physiol Heart Circ Physiol. 294(1) Pages: H441-8
  • Peer Reviewed
• [Journal Article] Visualisation of the effects of dilazep on rat afferent and efferent arterioles in vivo (2008)
  • Author(s): Nakamoto H, Ogasawara Y, Kajiya F
  • Journal Title: Hypertens Res. 31(2) Pages: 315-24
  • Peer Reviewed
• [Journal Article] Effects of sustained length-dependent activation on in situ cross-bridge dynamics in rat hearts (2007)
  • Author(s): Pearson JT, Shirai M, Tsuchimochi H, Schwenke DO, Ishida T, Kangawa K, Suga H, Yagi N
  • Journal Title: Biophys J. 93(12) Pages: 4319-29
  • Peer Reviewed
• [Journal Article] Adipose Tissue Remodeling and Malfunctioning in Obesity Revealed by in vivo Molecular Imaging (2007)
  • Author(s): Nishimura S, Manabe I, Nagasaki M, Hosoya Y, Kadowaki T, Nagai R, Sugiura S
  • Journal Title: Bioimages 15 Pages: 9-15
  • Peer Reviewed
• [Journal Article] Automation of Measurement Using a Flow-Through Type Differential pH Sensor Based on pH-FET (2007)
  • Author(s): Mohri S, Nakamura M, Naruse K
  • Journal Title: IEEJ Transactions on Sensors and Micromachines 127(8) Pages: 367-370
  • Peer Reviewed
• [Journal Article] Microheterogeneity of regional myocardial blood flows in low-perfused rat hearts evaluated by double-tracer digital radiography (2007)
  • Author(s): Matsumoto T, Asano T, Takemoto M, Tachibana H, Ogasawara Y, Kajiya F
  • Journal Title: Appl Radiat Isot. 65(8) Pages: 910-917
  • Peer Reviewed
• [Journal Article] Impaired NO-mediated vasodilation with increased superoxide but robust EDHF function in right ventricular arterial microvessels of pulmonary hypertensive rats (2007)
  • Author(s): Kajiya M, Hirota M, Inai Y, Kiyooka T, Morimoto T, Iwasaki T, Endo K, Mohri S, Shimizu J, Yada T, Ogasawara Y, Naruse K, Ohe T, Kajiya F
  • Journal Title: Am J Physiol Heart Circ Physiol. 292(6) Pages: H2737-44
  • Peer Reviewed
• [Journal Article] Important role of endogenous hydrogen peroxide in pacing-induced metabolic coronary vasodilation in dogs in vivo (2007)
  • Author(s): Yada T, Shimokawa H, Hiramatsu O, Shinozaki Y, Mori H, Goto M, Ogasawara Y, Kajiya F
  • Journal Title: J Am Coll Cardiol. 50(13) Pages: 1272-8
  • Peer Reviewed
• [Journal Article] Oxidized LDL specifically promotes the initiation of monocyte invasion during transendothelial migration with upregulated PECAM-1 and downregulated VE-cadherin on endothelial junctions (2007)
  • Author(s): Hashimoto K, Kataoka N, Nakamura E, Tsujioka K, Kajiya F
  • Journal Title: Atherosclerosis 194(2) Pages: e9-17
  • Peer Reviewed
• [Journal Article] Edaravone Preserves Coronary Microvascular Endothelial Function After Ischem/Reperfusion on the Beating Canine Heart In Vivo (2007)
  • Author(s): Sukmawan R, Yada T, Toyota E, Neishi Y, Kume T, Shinozaki Y, Mori H, Ogasawara Y, Kajiya F, Yoshida K
  • Journal Title: J Pharmacol Sci. 104 Pages: 341-348
  • Peer Reviewed
• [Journal Article] Celsior preserved cardiac mechanoenergetics better than popular solutions in canine hearts (2006)
  • Author(s): Oshima Y, Mohri S, Shimizu J, Iribe G, Imaoka T, Fujinaka W, Kiyooka T, Ishino K, Sano S, Kajiya F, Suga H
  • Journal Title: Ann Thorac Surg. 81(2) Pages: 658-64
  • Peer Reviewed
• [Journal Article] Membrane potential of rat ventricular myocytes responds to axial stretch in phase, amplitude and speed-dependent manners (2006)
  • Author(s): Nishimura S, Kawai Y, Nakajima T, Hosoya Y, Fujita H, Katoh M, Yamashita H, Nagai R, Sugiura S
  • Journal Title: Cardiovasc Res. 72(3) Pages: 403-11
  • Peer Reviewed
• [Journal Article] Microtubules modulate the stiffness of cardiomyocytes against shear stress (2006)
  • Author(s): Nishimura S, Nagai S, Katoh M, Yamashita H, Saeki Y, Okada J, Hisada T, Nagai R, Sugiura S
  • Journal Title: Circ Res. 98(1) Pages: 81-7
  • Peer Reviewed
• [Journal Article] Expression of green fluorescent protein impairs the force-generating ability of isolated rat ventricular cardiomyocytes (2006)
  • Author(s): Nishimura S, Nagai S, Sata M, Katoh M, Yamashita H, Saeki Y, Nagai R, Sugiura S
  • Journal Title: Mol Cell Biochem. 286(1-2) Pages: 59-65
  • Peer Reviewed
• [Journal Article] Carbon fiber technique for the investigation of single-cell mechanics in intact cardiac myocytes (2006)
  • Author(s): Sugiura S, Nishimura S, Yasuda S, Hosoya Y, Katoh K
  • Journal Title: Nat Protoc. 1(3) Pages: 1453-7
  • Peer Reviewed
• [Journal Article] Iris movement mediates vascular apoptosis during rat pupillary membrane regression (2006)
  • Author(s): Morizane Y, Mohri S, Kosaka J, Tone S, Kiyooka T, Miyasaka T, Shimizu J, Ogasawara Y, Shiraga F, Minatogawa Y, Sasaki J, Ohtsuki H, Kajiya F
  • Journal Title: Am J Physiol Regul Integr Comp Physiol 290(3) Pages: R819-25
  • Peer Reviewed
• [Journal Article] Cardioprotective Role of Endogenous Hydrogen Peroxide during Ischemia-Reperfusion Injury in Canine Coronary Microcirculation in Vivo (2006)
  • Author(s): Yada T, Shimokawa H, Hiramatsu O, Haruna Y, Morita Y, Kashihara N, Shinozaki Y, Mori H, Goto M, Ogasawara Y, Kajiya F
  • Journal Title: Am J Physiol Heart Circ Physiol 291(3) Pages: H1138-1146
  • Peer Reviewed
• [Journal Article] Cardioprotective effect of hydroxyfasudil as a specific Rho-kinase inhibitor, on ischemia-reperfusion injury in canine coronary microvessels in vivo (2006)
  • Author(s): Yada T, Shimokawa H, Kajiya F
  • Journal Title: Clin Hemorheol Microcirc 34(1-2) Pages: 177-83
  • Peer Reviewed
• [Journal Article] Prediction of functional recovery of 60-minute warm ischemic hearts from asphyxiated canine non-heart-beating donors (2006)
  • Author(s): Hirota M, Ishino K, Fukumatsu I, Yoshida K, Mohri S, Shimizu J, Kajiya F
  • Journal Title: J Heart Lung Transplant 25(3) Pages: 339-44
  • Peer Reviewed
• [Journal Article] Measurements of CO2, lactic acid and sodium bicarbonate secreted by cultured cells using a flow- though type pH/CO2 sensor system based on ISFET (2005)
  • Author(s): Mohri S, Shimizu J, Goda N, Miyasaka T, Fujita A, Nakamura M, Kajiya F
  • Journal Title: Sensors and Actuators B 115 Pages: 519-525
  • Peer Reviewed
• [Journal Article] Contractile dysfunction of cardiomyopathic hamster myocytes is pronounced under high load conditions (2005)
  • Author(s): Nishimura S, Yamashita H, Katoh M, Yamada KP, Sunagawa K, Saeki Y, Ohnuki Y, Nagai R, Sugiura S
  • Journal Title: J Mol Cell Cardiol 39(2) Pages: 231-9
  • Peer Reviewed
• [Journal Article] Cardiac hemodynamics, coronary circulation and interventional cardiology (2005)
  • Author(s): Kajiya F, Zamir M, Carlier S
  • Journal Title: Ann Biomed Eng. 33(12)(Review) Pages: 1728-34
  • Peer Reviewed
• [Presentation] Local Dynamic Recruitment of Endothelial PECAM-1 to Transmigrating Monocytes (2008)
  • Author(s): Kataoka N.
  • Organizer: 13th International Conference on Biomedical Engineering
  • Place of Presentation: Singapore
  • Year and Date: 2008-12-03
• [Presentation] Collaboration among academia, industry and government on integration from nanotechnology to systems biology (2008)
  • Author(s): Kajiya F.
  • Organizer: International Physiome Symposium 2008
  • Place of Presentation: Seoul, Korea
  • Year and Date: 2008-04-10
• [Presentation] Effects of hyperlipidemia on coronary endothelial function evaluated by a cathter-type no sensor and coronary flow regulation (2006)
  • Author(s): Goto M.
  • Organizer: 17th Cardiovascular System Dynamics Society 2006
  • Place of Presentation: Netherlands
  • Year and Date: 20060909-12
• [Presentation] Impaired coronary capillary flow with decreased glycocalyx thickness and irregular inner wall in right ventricle of pulmonary hypertensive rats (2006)
  • Author(s): Kajiya M.
  • Organizer: 17th Cardiovascular System Dynamics Society 2006
  • Place of Presentation: Netherlands
  • Year and Date: 20060909-12
• [Presentation] Coronary microcirculation in the beating heart (2006)
  • Author(s): Kajiya F.
  • Organizer: 17th Cardiovascular System Dynamics Society 2006
  • Place of Presentation: Netherlands
  • Year and Date: 20060909-12
• [Presentation] Important Role of Cu/An-SOD in the Synthesis of Endothelium-Derived Hyperpolarizing Factor during Reactive Hyperemia in Mouse Mesenteric Microvessels in Vivo (2006)
  • Author(s): Yada T.
  • Organizer: The 70th Anniversary Annual Scientific Meeting of the Japanese Circulation Society
  • Place of Presentation: Nagoya
  • Year and Date: 20060324-26
• [Presentation] Crucial Role of Hydrogen Peroxide as an Endogenous EDHF during Pacing-Induced Metabolic Dilatation in Canine Coronary Microvessels in Vivo (2006)
  • Author(s): Yada T.
  • Organizer: The 70th Anniversary Annual Scientific Meeting of the Japanese Circulation Society
  • Place of Presentation: Nagoya
  • Year and Date: 20060324-26
• [Presentation] Oxidized LDL Specifically Promotes the Initiation of Monocyte Invasion at Cell-Cell Junctions by Changing the Intracellular Distribution of PECAM-1 (2006)
  • Author(s): Hashimoto K.
  • Organizer: The 70th Anniversary Annual Scientific Meeting of the Japanese Circulation Society
  • Place of Presentation: Nagoya
  • Year and Date: 20060324-26
• [Presentation] Impaired Coronary Capillary Flow with Decreased Glycocalyx Thickness and Irregular Inner Wall Remodeling in Right Ventricle of Pulmonary Hypertensive Rats (2006)
  • Author(s): Kajiya M.
  • Organizer: The 70th Anniversary Annual Scientific Meeting of the Japanese Circulation Society
  • Place of Presentation: Nagoya
  • Year and Date: 20060324-26
• [Presentation] Robust and Harmonic EDHF Functions of Arterioles and Venules Preserve Capillary Hemodynamics in the Early Stage of Hypertension (2006)
  • Author(s): Hirota M, Inai Y, Kajiya M, Kiyooka T, Mohri S, Tedoriya T, Sano S, Kajiya F
  • Organizer: The 70th Anniversary Annual Scientific Meeting of the Japanese Circulation Society
  • Place of Presentation: Nagoya
  • Year and Date: 20060324-26
• [Presentation] Cardioprotective Role of Hydrogen Peoxide as an Endogenous EDHF during Ischemia-reperfusion Injury in Canine Coronary Microvessels in Vivo (2006)
  • Author(s): Yada T, Shimokawa H, Hiramatsu O, Shinozaki Y, Mori H, Goto M, Ogasawara Y, Kajiya F
  • Organizer: The 70th Anniversary Annual Scientific Meeting of the Japanese Circulation Society
  • Place of Presentation: Nagoya
  • Year and Date: 20060324-26
• [Presentation] Pivotal Contribution of Platelets to Disseminated Intravascular Coagulation (2006)
  • Author(s): Nakamoto H, Ogasawara Y, Kajiya F
  • Organizer: The 70th Anniversary Annual Scientific Meeting of the Japanese Circulation Society
  • Place of Presentation: Nagoya
  • Year and Date: 20060324-26
• [Presentation] Oxidized LDL specifically promotes monocyte post-adhesion dynamics on endothelium, but not invasion itself at junctions, or subendothelial migration after invasion. (2005)
  • Author(s): Hashimoto K.
  • Organizer: 12th International Conference on Biomedical Engineering 2005
  • Place of Presentation: Singapore
  • Year and Date: 20051207-10
• [Presentation] Flow visualization study of the endothelialized glass aneurysm model implanting into the canine carotid artery (2005)
  • Author(s): Ujiie H.
  • Organizer: 12th International Conference on Biomedical Engineering 2005
  • Place of Presentation: Singapore
  • Year and Date: 20051207-10
• [Presentation] Impaired coronary microvascular endothelial cell function with increased superoxide in right ventricle of pulmonary hypertensive rats (2005)
  • Author(s): Kajiya M.
  • Organizer: 12th International Conference on Biomedical Engineering 2005
  • Place of Presentation: Singapore
  • Year and Date: 20051207-10
• [Presentation] Robust and Harmonic EDHF Functions of Arterioles and Venues Preserve Capillary Hemodynamics in Early Stage of Hypertension (2005)
  • Author(s): Hirota M.
  • Organizer: American Heart Association, scientific sessions 2005
  • Place of Presentation: Dallas, U.S.A
  • Year and Date: 2005-11-16
• [Presentation] Impaired Coronary Capillary Hemodynamics with Decreased Glycocalyx Thickness and Irregular Inner Wall in Right Ventricle of Pulmonary Hypertensive Rats (2005)
  • Author(s): Kajiya M.
  • Organizer: American Heart Association, scientific sessions 2005
  • Place of Presentation: Dallas, U.S.A
  • Year and Date: 2005-11-16