2007 Fiscal Year Final Research Report Summary
Construction of ultrahigh-density haplotyping devices using characteristics in nanospace
| Project/Area Number |
17201032
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Microdevices/Nanodevices
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| Research Institution | Nagoya University |
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Principal Investigator |
BABA Yoshinobu Nagoya University, Faculty of Engineering, Professor (30183916)
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| Co-Investigator(Kenkyū-buntansha) |
KAJI Noritada Nagoya University, Faculty of Engineering, Assistant Professor (90402479)
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| Project Period (FY) |
2005 – 2007
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| Keywords | Micro-and Nanodevices / Genome / Nanobio / Fluid Simulation |
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| Research Abstract |
We have developed ultrahigh-density haplotyping devices composed of ultra-fast genome analysis device, ultra-sensitive DNA detection device, and ultra-small reaction chamber system for genome analysis. In the first yean essential techniques in these component devices were developed. As a result, nanostructures inside microchannel were optimized for DNA sieving matrix and high sensitive detection using a novel DNA labeling method was achieved. Furthermore, it was demonstrated that the inkjet-type injection method did work as a DNA injectr. In the seccond year we investigated how to army microchannel in high density Since straight type microchannel is most suitable for making high-density microchannel array, combination of nanostructures and microchannel was optimized and ink-jet-type injector was mounted on the straight miceothannel We elucidated that this combination worked well as a DNA analysis device. In the last year final evaluation of the prototype device was performed. Combining all components that have been developed so fat highly integrated and high-density device was fabricated on a chip as a prototype device. Regarding to inkjet-type injector applied voltage, frequency, and wave pattern was optimized to reduce the distribution of volume of a single aliquot. After this process, we could inject 10 pL DNA sample into the straight type microchannel and electrophoretic DNA separation was successfully done. Although high-density haplotyping devices has same problems such as physical interference of peripheral equipment, we demonstrated highly integrated and high-density haplotyping devices could be realized using the above developed techniques.
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