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2008 Fiscal Year Final Research Report

Evolution of microtubule organizing system in land plants in terms of dynamics and nano-structures of microtubule ends

Research Project

  • PDF
Project/Area Number 17207006
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Morphology/Structure
Research InstitutionUniversity of Hyogo

Principal Investigator

MINEYUKI Yoshinobu  University of Hyogo, 大学院・生命理学研究科, 教授 (30219703)

Co-Investigator(Kenkyū-buntansha) 唐原 一郎  富山大学, 大学院・理工学研究科, 准教授 (60283058)
中井 朋則  兵庫県立大学, 大学院・生命理学研究科, 助教 (60347531)
Co-Investigator(Renkei-kenkyūsha) STAEHELIN L. Andrew  コロラド大学, MCD Biology, 教授
Project Period (FY) 2005 – 2008
Keywords植物 / ナノマシン / 細胞・組織 / 生体分子 / ガンマチューブリン / 微小管形成中心 / 電子線トモグラフィー
Research Abstract

動物は微小管形成の制御装置として中心体を持つが、植物は中心体なしで微小管形成を行っている。本研究では、微小管の重合開始に必要なγチューブリン分子とγチューブリンが作る微小管端のキャップ構造に注目して、植物特有の微小管形成システムは何かを調べた。その結果、被子植物ではキャップ構造が不安定なことが示唆できた。また、コケ・シダ・裸子植物で保存され、被子植物で変化するγチューブリン分子表面に存在する十数個のアミノ酸が見つかった。キャップ構造の安定性とγチューブリン構造の変化との関係は今後の課題となった。

  • Research Products

    (27 results)

All 2009 2008 2007 2006 2005 2004 Other

All Journal Article (6 results) (of which Peer Reviewed: 3 results) Presentation (17 results) Book (3 results) Remarks (1 results)

  • [Journal Article] The preprophase band is a localized center of clathrin-mediated endocytosis in late prophase cells of the onion cotyledon epidermis2009

    • Author(s)
      Karahara, I., Suda, J., Tahara, H., Yokota, E., Shimmen, T., Misaki, K., Yonemura, S., Staehelin, L.A., and Mineyuki, Y
    • Journal Title

      Plant J 57

      Pages: 813-831

    • Peer Reviewed
  • [Journal Article] Plant microtubule studies : past and present2007

    • Author(s)
      Mineyuki, Y
    • Journal Title

      J. Plant Res 120

      Pages: 45-51

    • Peer Reviewed
  • [Journal Article] γ-Tubulin-like molecules in the mouse duodenal epithelium2007

    • Author(s)
      Suzaki, E., Nomura, R., Horio, T., Mineyuki, Y., and Kataoka, K
    • Journal Title

      Histochem. Cell Biol 128

      Pages: 175-182

    • Peer Reviewed
  • [Journal Article] 電子線トモグラフィーとは何か : ナノスケールでの3Dバイオイメージング2007

    • Author(s)
      唐原一郎, 須田甚将, 峰雪芳宣
    • Journal Title

      細胞工学 26

      Pages: 696-701

  • [Journal Article] Membrane?associated microtubuleorganizing centers in basal land plants2005

    • Author(s)
      Mineyuki, Y., and Shimamura, M
    • Journal Title

      Cytologia 70

      Pages: i-ii

  • [Journal Article] Quantitative analysis of vesicles in the preprophase band by electron tomography

    • Author(s)
      Karahara, I., Suda, J., Staehelin, L.A., and Mineyuki, Y
    • Journal Title

      Cytologia (in press)

  • [Presentation] Changes in function and amino acid residues of γ-tubulin in the evolution of green plants2009

    • Author(s)
      Miyamoto, Y., Horio, T., Yamauchi, D., Okada, T., Sekita, S., Futamura, N., Shinohara, K., Fujimoto, Y., Nakai, T., Shimmen, T., Shimamura, M., Akashi, T., and Mineyuki, Y
    • Organizer
      第61回日本細胞生物学会大会
    • Place of Presentation
      名古屋市
    • Year and Date
      20090602-04
  • [Presentation] Electron tomographic analysis of the plant cell cortex : The preprophase band is a localized center of clathrin-mediated endocytosis2009

    • Author(s)
      Karahara, I., Suda, J., Tahara, H., Yokota, E., Shimmen, T., Misaki, K., Yonemura, S., Staehelin, L., and Mineyuki, Y
    • Organizer
      Asia-Pacific Congress on Electron Tomography
    • Place of Presentation
      Brisbane, Australia
    • Year and Date
      20090131-0204
  • [Presentation] Electron tomographic analysis of microtubulemicrofilament interactions in the epidermal cell cortex of high pressure frozen cotyledons2009

    • Author(s)
      Takeuchi, M., Karahara, I., Kajimura, N., Takaoka, A., Misaki, K., Yonemura, S., Staehelin, L., and Mineyuki, Y
    • Organizer
      Asia-Pacific Congress on Electron Tomography
    • Place of Presentation
      Brisbane, Australia
    • Year and Date
      20090131-0204
  • [Presentation] Preprophase band is a localized center of clathrin-mediated endocytosis in late prophase of onion cotyledon epidermis2008

    • Author(s)
      Karahara, I., Suda, J., Masuta, Y., Tahara, H., Yokota, E., Shimmen, T., Misaki, K., Yonemura, S., Staehelin, L., and Mineyuki, Y
    • Organizer
      9th Asia-Pacific Microscopy Conference
    • Place of Presentation
      Jeju, Republic of Korea
    • Year and Date
      20081102-07
  • [Presentation] Plant γ-tubulins have gradually lost the function that is essential for unikonts cells during their evolution2008

    • Author(s)
      Miyamoto, Y., Yamauchi, D., Kuno, R., Nakai, T., Futamura, N., Shinohara, K., Shimmen, T., Shimamura, M., Akashi, T., Horio, T., and Mineyuki, Y
    • Organizer
      2nd International Symposium on Bio-nanosystems
    • Place of Presentation
      Tokyo
    • Year and Date
      20081031-1102
  • [Presentation] 植物細胞表層の電子線トモグラフィー解析2008

    • Author(s)
      唐原一郎, 峰雪芳宣
    • Organizer
      日本植物学会第72回大会 シンポジウム
    • Place of Presentation
      高知
    • Year and Date
      20080925-27
  • [Presentation] 加圧凍結・2軸電子線トモグラフィー法による微小管構築機構の解析2008

    • Author(s)
      峰雪芳宣, 唐原一郎
    • Organizer
      第49回日本植物生理学会年会シンポジウム"植物細胞の内骨格と外骨格"
    • Place of Presentation
      札幌
    • Year and Date
      20080320-22
  • [Presentation] Analysis of Membrane/Cytoskeletal Systems in Plant Cell Cortex2008

    • Author(s)
      Mineyuki, Y
    • Organizer
      International Symposium on Picobiology
    • Place of Presentation
      Kamigori, Hyogo
    • Year and Date
      20080318-19
  • [Presentation] 電子線トモグラフィー-分裂準備帯における膜系の3次元微細構造解析-2007

    • Author(s)
      唐原一郎, 須田甚将, Staehelin, L.A., 峰雪芳宣
    • Organizer
      第48回日本植物生理学会年会シンポジウム"植物細胞を観る??植物バイオイメージング最前線-"
    • Place of Presentation
      松山
    • Year and Date
      20070328-30
  • [Presentation] Electron tomographic analysis of preprophase band2007

    • Author(s)
      Mineyuki, Y., and Karahara, I
    • Organizer
      Andrew Staehelin?s Retirement Symposium
    • Place of Presentation
      Boulder, USA
    • Year and Date
      2007-06-06
  • [Presentation] Electron tomographic analysis of cortical microtubule ends in plant interphase and prophase cells preserved by high-pressure freezing2006

    • Author(s)
      Mineyuki, Y., Karahara, I., Suda, J., and Staehelin, L.A
    • Organizer
      The 16th Internatinal Microscopy Congress
    • Place of Presentation
      Sapporo
    • Year and Date
      20060903-08
  • [Presentation] Effects of cytochalasin D on vesicle transport at the preprophase band analyzed by electron tomography2006

    • Author(s)
      Suda, J., Karahara, I., Staehelin, L.A., and Mineyuki, Y
    • Organizer
      The 16th Internatinal Microscopy Congress
    • Place of Presentation
      Sapporo
    • Year and Date
      20060903-08
  • [Presentation] Electron tomographic analysis of cortical microtubule ends in plant interphase and prophase cells preserved by high-pressure freezing2006

    • Author(s)
      Mineyuki, Y., Karahara, I., Suda, J., and Staehelin, L.A
    • Organizer
      The 16th Gordon Research Conference Plant & Fungal Cytoskeleton
    • Place of Presentation
      Andover, New Hampshire, USA
    • Year and Date
      20060820-25
  • [Presentation] 細胞分裂パターン制御に関与する細胞骨格・膜からなるナノシステムの解析2006

    • Author(s)
      峰雪芳宣
    • Organizer
      第66回 京阪神地区 植物細胞生理学談話会
    • Place of Presentation
      大阪市
    • Year and Date
      2006-03-25
  • [Presentation] 加圧凍結・電子線トモグラフィーを使った微小管端構造の解析2005

    • Author(s)
      峰雪芳宣, 唐原一郎
    • Organizer
      日本植物学会第69回大会シンポジウム"微小管構築の分子機構解明への新展開"
    • Place of Presentation
      富山市
    • Year and Date
      20050920-22
  • [Presentation] 加圧凍結・2軸電子線トモグラフィー法による微小管ダイナミクス制御ナノマシンの解析2005

    • Author(s)
      峰雪芳宣, 唐原一郎
    • Organizer
      日本顕微鏡学会第61回学術講演会 シンポジウム 第2部"電子線トモグラフィーと細胞生物学"
    • Place of Presentation
      つくば市
    • Year and Date
      20050601-03
  • [Presentation] Membrane Associated Microtubule System in Lower Land Plants Holds Clues to Evolution of the Cytokinetic Apparatus of Division Site Determination in Flowering Plants2004

    • Author(s)
      Mineyuki, Y., and Shimamura, M
    • Organizer
      The Americal Society for Cell Biology 2004 Summer Meeting "Cytokinesis"
    • Place of Presentation
      Vermont, USA
    • Year and Date
      20040722-25
  • [Book] 電子線トモグラフィーとは何か : ナノスケールでの3D バイオイメージング. 電子顕微鏡で読み解く生命のなぞ ナノワールドに迫るパワフル技術入門2008

    • Author(s)
      唐原一郎, 須田甚将, 峰雪芳宣
    • Total Pages
      77-82
    • Publisher
      藤本豊士, 山本章嗣編 (東京 : 秀潤社)
  • [Book] 電子線トモグラフィー. 細胞工学別冊植物細胞工学シリーズ22 植物の細胞を観る実験プロトコール2006

    • Author(s)
      峰雪芳宣, 唐原一郎, 須田甚将
    • Total Pages
      95-97
    • Publisher
      福田裕穂, 西村幹生, 中野明彦編(秀潤社)
  • [Book] Methods in Molecular Biology Series, S. Schwartzbach and T. Osafune, eds(Totoa, NJ : The Humana Press Inc.)(in press)

    • Author(s)
      Takeuchi, M., Takabe, K., and Mineyuki, Y
    • Publisher
      Immunoelectron microscopy of cryofixed and freeze- substituted plant tissues. In Immuno-electron Microscopy : Methods and Protocols
  • [Remarks]

    • URL

      http://www.sci.u-hyogo.ac.jp/life/biosynth/index-j.html

URL: 

Published: 2010-06-10   Modified: 2016-04-21  

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