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2007 Fiscal Year Final Research Report Summary

New approaches to functional elucidation of unknown genes related to lipid metabolism : combination of mutational approaches using C. elegans and metabolite analyses using mass spectrometry

Research Project

Project/Area Number 17207008
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Functional biochemistry
Research InstitutionThe University of Tokyo

Principal Investigator

ARAI Hiroyuki  The University of Tokyo, Department of Healtri Chemistry, Graduate School of Pharmaceutical Sciences, Professor (40167987)

Co-Investigator(Kenkyū-buntansha) INOUE Takao  The University of Tokyo, Graduate School of Pharmaceutical Sciences, Department of Health Chemistry, Assistant professor (50361605)
Project Period (FY) 2005 – 2007
KeywordsC. elegans / phosnholinid / fatty acid / acvltransferase
Research Abstract

Various kinds of fatty acids are distributed in membrane phospholipids in mammalian cells and tissues. The fatty acyl residues of individual phospholipids appear to be under strict metabolic regulation. In general, saturated fatty acids are esterified at the sn-1 position, whereas polyunsaturated fatty acids (PUFAs), such as arachidonic acid, are commonly found at the sn-2 position. The rapid turnover of the sn-2 acyl chains of glycerophospholipids was originally described by Lands, who proposed that acyl moieties of membrane phospholipids are rapidly metabolized by the action of phospholipase A_2s and subsequently by lysophospholipid acyltransferases (Lands' cycle); however the genes involved in this turnover have not been reported.
In this project, we established a screening system to identify genes required for utilization of exogenous PUFAs in C. elegans and found that an uncharacterized gene, which we named mboa-7, is required for incorporation of PUFAs into phosphatidylinositol (P … More I). Incorporation of exogenous PUFA into PI of the living worms and lysoPI acyltransferase (LPIAT) activity in the microsomes were greatly reduced in mboa-7 mutants. Furthermore, the membrane fractions of transgenic worms expressing recombinant mboa-7 and its human homologue exhibited remarkably increased LPIAT activity. mboa-7 encodes a member of the membrane-bound O-acyltransferase family, suggesting that mboa-7 is LPIAT. We also identified an acyltransferase which catalyzes acyltransferase activity toward lysoPC, lysoPS, and lysoPE.
In regards to phospholipase A_2s, we generated knockout mice of PAF-AH (II), which hydrolyzes oxidatively fragmented fatty acyl chains attached to phospholipids and was predominantly expressed in epithelial cells such as kidney proximal and distal tubules, intestinal column epithelium and hepatocytes. Pafah2^<-/-> mice developed normally and were phenotypically indistinguishable from wild-type mice. However, mouse embryonic fibroblasts derived from Pafah2% mice were more sensitive to tert-butylhydroperoxide treatment than those derived from wild-type mice. When carbon tetrachloride (CCl_4) was injected into mice, Pafah2^<-/-> mice showed a delay in hepatic injury recovery. Moreover, following CCl_4 administration, liver levels of the esterified form of 8-iso-PGF_2a, a known in vitro substrate of PAF-AH (II), were higher in Pafah2^<-/-> mice than in wild-type mice. These results indicate that PAF-AH (II) is involved in the metabolism of esterified 8-iso-PGF_2a and protects tissue from oxidative stress-induced injury. Less

  • Research Products

    (112 results)

All 2008 2007 2006 2005

All Journal Article (26 results) (of which Peer Reviewed: 12 results) Presentation (86 results)

  • [Journal Article] Caenorhabditis elegans mboa-7, a Member of the MBOAT Family, Is Required for Selective Incorporation of Polyunsaturated. Fatty Acids into Phosphatidylinositol2008

    • Author(s)
      Lee HC
    • Journal Title

      Mol. Biol. Cell 19

      Pages: 1174-1184

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Protection against oxidative stress-induced hepatic injury by intracellular type II platelet-activating factor acetylhydrolase by metabolism of oxidized phospholipids in vivo.2008

    • Author(s)
      Kono N.
    • Journal Title

      J. Biol. Chem. 283

      Pages: 1628-1636

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] beta-Catenin asymmetry is regulated by PLA_1 and retrograde traffic in C. elegans stem cell divisions2008

    • Author(s)
      Kanamori T.
    • Journal Title

      Embo J. (In press)

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] A member of the membrane-bound O-acyltransferase (MBOAT) family encodes a lysophospholipid acyltransferase with broad substrate specificity2008

    • Author(s)
      Matsuda S.
    • Journal Title

      Genes to Cells (In press)

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] C. elegans mboa-7, a member of the MBOAT family, is required for selective incorporation of polyunsaturated fatty acids into phosphatidylinositol2008

    • Author(s)
      Lee H. -C., Inoue T., Imae R., Kono N., Shirae S., Matsuda S., Gengyo-Ando K., Mitani S., Arai H.
    • Journal Title

      Mol. Biol. Cell 19

      Pages: 1174-1184

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Protection against oxidative stress-induced hepatic injury by intracellular type II PAF-acetylhydrolase by metabolism of oxidized phospholipids in vivo2008

    • Author(s)
      Kono N., Inoue T., Yoshida Y., Sato H., Matsusue T., Itabe H., Niki E., Aoki J., Arai H.
    • Journal Title

      J. Biol. Chem 283

      Pages: 1628-1636

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] b-Catenin asymmetry is regulated by PLA1 andretrograde traffic in C. elegans stem cell divisions2008

    • Author(s)
      Kanamori T., Inoue T., Gengyo-Ando K., Sakamoto T., Tsujimoto M., Mitani S., Sawa, H., Aoki J., Arai H.
    • Journal Title

      EMBO J. (in press)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] A member of the membrane-bound O-acyltransferase (MBOAT) family encodes a lvsonhosnholinid acvltransferase with broad substrate specificity2008

    • Author(s)
      Matsuda S., Inoue T., Lee H. -C., Kono N., Tanaka E, Gengyo-Ando K., Mitani S., Arai H.
    • Journal Title

      Genes to Cells (in press)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Neuroprotective Role of Transgenic PAF-Acetylhydrolase II in Mouse Models of Focal Cerebral Ischemia.2007

    • Author(s)
      Umemura K.
    • Journal Title

      Stroke 38

      Pages: 1063-1068

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Strucuture and function of extracellular phospholipase A l belonging to the pancre atic lipase gene family.2007

    • Author(s)
      Aoki J.
    • Journal Title

      Biochimie 89

      Pages: 197-204

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Type I platelet-activating factor acetylhydrolase catalytic subunit overexpression in duces pleiomorphic nuclei and centrosome amplification.2007

    • Author(s)
      Yamaguchi N.
    • Journal Title

      Genes to Cells 12

      Pages: 1153-1161

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Phospholipase A(1)assays using a radiolabeled substrate and mass spectrometry.2007

    • Author(s)
      Morikawa R.
    • Journal Title

      Methods Enzymol. 434

      Pages: 1-13

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Transgenic expression of group V, but not group X, secreted phospholipase A2 in mice leads to neonatal lethality because of lung dysfunction2007

    • Author(s)
      Ohtsuki M, Taketomi Y, Arata S, Masuda S, Ishikawa Y, Ishii T, Takanezawa Y, Aoki J, Arai H, Yamamoto K, Kudo I, Murakami M.
    • Journal Title

      J. Biol. Chem 281

      Pages: 36420-36433

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Neuroprotective role of transgenic PAF-acetylhydrolase II in mouse models of focal cerebral ischemia2007

    • Author(s)
      Umemura K., Kato I., Hirashima Y., Ishii Y., Inoue T., Aoki J., Kono N., Oya T., Hayashi N., Hamada H., Endo S., Oda M., Arai H., Kinouchi H., Hiraga K.
    • Journal Title

      Stroke 38

      Pages: 1063-108

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Strucuture and function of extracellular phospholipase A 1 belonging to the pancreatic lipase gene family2007

    • Author(s)
      Aoki J., Inoue A., Makide K., Saiki N., Arai H.
    • Journal Title

      Biochimie 89

      Pages: 197-204

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Type I platelet-activating factor acetylhydrolase catalytic subunit overexpression induces pleiomorphic nuclei and centrosome amplification2007

    • Author(s)
      Yamaguchi N., Koizumi H., Aoki J., Natori Y., Nishikawa K., Natori Y., Takanezawa Y., Arai H.
    • Journal Title

      Genes to Cells 12

      Pages: 1153-1161

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Phospholipase Al assays using a radio-labeled substrate and mass spectrometry2007

    • Author(s)
      Morikawa R., Tsujimoto M., Arai H., Aoki J.
    • Journal Title

      Methods in Enzymol 434

      Pages: 1-13

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] A Novel Sulfotransferase Abundantly Expressed in the Dauer Larvae of Caenorh abditis elegans2006

    • Author(s)
      Kenji H.
    • Journal Title

      J. Biochem(Tokyo) 139

      Pages: 355-362

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Transgenic Expression of Group V, but not Group X, Secreted Phospholipase A2 in Mice Leads to Neonatal Lethality due to Lung Dysfunction2006

    • Author(s)
      Ohtsuki M.
    • Journal Title

      J. Biol. Chem. 281

      Pages: 36420-36433

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Tamura H. A novel sulfotransferase abundantly expressed in the dauer larvae of Caenorhabditis elegans2006

    • Author(s)
      Hattori K., Inoue M., Inoue T., Arai H.
    • Journal Title

      J. Biochem. (Tokyo) 139

      Pages: 55-62

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Neuronal Expression and Neuritogenic Action of Group X Secreted Phospholipase A22005

    • Author(s)
      Masuda S.
    • Journal Title

      J. Biol. Chem. 280

      Pages: 23203-23214

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] New approaches to functional elucidation of unknown genesrelated to lipid metabolism: combination of mutational approaches using C. elegans and metabolite analyses using mass spectrometry (Review)2005

    • Author(s)
      Inoue T.
    • Journal Title

      実験医学 23

      Pages: 977-985

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Group VIB Ca2+-independent phospholipase A2(iPLA2γ)promotes cellular membrane hydrolysis and prostaglandin production in a manner distinct from other intracellular phospholipase A2s.2005

    • Author(s)
      Murakami M.
    • Journal Title

      J. Biol. Chem. 280

      Pages: 14028-14041

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Neuronal expression and neuritogenic action of group X secreted phospholipase A22005

    • Author(s)
      Masuda S., Murakami M., Takanezawa Y., Aoki J., Arai H., Ishikawa Y., Ishii T., Arioka M., Kudo I.
    • Journal Title

      J. Biol. Chem 280

      Pages: 23203-23214

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Arai H. New approaches to functional elucidation of unknown genes related to lipid metabolism : combination of mutational approaches using C. elegans and metabolite analyses using mass spectrometry (Review)2005

    • Author(s)
      Inoue T., Takanezawa Y.
    • Journal Title

      Jikkenn Igaku 23

      Pages: 977-985

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Group VIB Ca2+-independent phospholipase A2 (iPLA2γ) promotes cellular membrane hydrolysis and prostaglandin production in a manner distinct from other intracellular phospholipase A2s2005

    • Author(s)
      Murakami M., Masuda S., Ueda-Semmyo K., Yoda E., Kuwata, H. Takanezawa Y., Aoki J., Arai H., Sumimoto H., Ishikawa Y., Ishii T., Nakatani Y., Kudo I.
    • Journal Title

      J. Biol. Chem 280

      Pages: 14028-14041

    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Metabolism of F2-isoprostane-containing phospholipids by type II PAF acetylhydrolase2008

    • Author(s)
      Nozomu Kono
    • Organizer
      BOSHD2008
    • Place of Presentation
      Osaka, Japan
    • Year and Date
      2008-01-17
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Metabolism of F2-isoprostane-containing phospholipids by type II PAF acetvlhvdrolase2008

    • Author(s)
      Nozomu Kono
    • Organizer
      BOSHD2008
    • Place of Presentation
      Osaka, Japan
    • Year and Date
      2008-01-17
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] C. elegans mboa-7, a Member of the MBOAT family, is Required for Selective Incorporation of Polyunsaturated Fatty Acids into Phosphatidylinositol2008

    • Author(s)
      Takao Inoue
    • Organizer
      Keystone Symposia
    • Place of Presentation
      Big Sky, Montana
    • Year and Date
      2008-01-14
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Analysis of Oxysterol binding protein in mammalian cells2007

    • Author(s)
      Taki Nishimura, Takao Inoue, Hiroyuki Arai
    • Organizer
      127th Annual Meeting of the Pharmaceutical Society of Japan
    • Place of Presentation
      Toyama, Japan
    • Year and Date
      20070328-30
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Neuronal function of an acyltransferase-like gene in C. elegans2007

    • Author(s)
      原直子
    • Organizer
      BMB2007
    • Place of Presentation
      横浜
    • Year and Date
      2007-12-14
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Neuronal function of an acyltransferase-like gene in C. elegans2007

    • Author(s)
      Naoko Hara
    • Organizer
      BMB2007
    • Place of Presentation
      Yokohama, Japan
    • Year and Date
      2007-12-14
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] A large-scale RNAi screen for genes involved in incorporation of exogenous PUFAs into phospholipids in C. elegans2007

    • Author(s)
      井上貴雄
    • Organizer
      BMB2007
    • Place of Presentation
      横浜
    • Year and Date
      2007-12-12
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] 細胞内型ホスホリパーゼA1 KIAA0725のドメイン解析2007

    • Author(s)
      森川 麗
    • Organizer
      BMB2007
    • Place of Presentation
      横浜
    • Year and Date
      2007-12-12
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] A genome-wide RNAi screen to identify genes required for incorporation of exogenous PUFAs in C. elegans2007

    • Author(s)
      Hyeon-Cheol Lee
    • Organizer
      BMB2007
    • Place of Presentation
      横浜
    • Year and Date
      2007-12-12
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] In vivo incorporation of radiolabeled fatty acids into. C. elegans2007

    • Author(s)
      松田真治
    • Organizer
      BMB2007
    • Place of Presentation
      横浜
    • Year and Date
      2007-12-12
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] A large-scale RNAi screen for genes involved in incorporation of exogenous PUFAs into phospholipids in C. elegans2007

    • Author(s)
      Takao Inoue
    • Organizer
      BMB2007
    • Place of Presentation
      Yokohama, Japan
    • Year and Date
      2007-12-12
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] A genome-wide RNAi screen to identify genes required for incorporation of exogenous PUFAs in C. elegans2007

    • Author(s)
      Hyeon-Cheol Lee
    • Organizer
      BMB2007
    • Place of Presentation
      Yokohama, Japan
    • Year and Date
      2007-12-12
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] In vivo incorporation of radiolabeled fatty acids into C. elegans2007

    • Author(s)
      Shinji Matsuda
    • Organizer
      BMB2007
    • Place of Presentation
      Yokohama, Japan
    • Year and Date
      2007-12-12
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Regulation of asymmetric divisions of C.elegans lateral epidermis by phospholip ase Al and retrograde trafficking2007

    • Author(s)
      Takahiro Kanamori
    • Organizer
      BMB2007
    • Place of Presentation
      横浜
    • Year and Date
      2007-12-11
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] ANALYSIS OF TYPE II PAF ACETYLHYDROLASE-DEFICIENT MICE2007

    • Author(s)
      Nozomu Kono
    • Organizer
      BMB2007
    • Place of Presentation
      横浜
    • Year and Date
      2007-12-11
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Regulation of asymmetric divisions of C. elegans lateral epidermis by phospholipase A1 and retrograde trafficking2007

    • Author(s)
      Takahiro Kanamori
    • Organizer
      BMB2007
    • Place of Presentation
      Yokohama, Japan
    • Year and Date
      2007-12-11
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] ANALYSIS OF TYPE II PAF ACETYLHYDROLASE-DEFICIENT MICE2007

    • Author(s)
      Nozomu Kono
    • Organizer
      BMB2007
    • Place of Presentation
      Yokohama, Japan
    • Year and Date
      2007-12-11
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] 線虫C.elegansを用いた高度不飽和脂肪酸の生理的意義の解明2007

    • Author(s)
      Hiroyuki Arai
    • Organizer
      日本薬学会関東支部第32回学術講演会
    • Place of Presentation
      東京
    • Year and Date
      2007-10-20
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] PAF-Acetylhydrolase as an Oxidized Phospholipids-Selective Phospholipase2007

    • Author(s)
      Hiroyuki Arai
    • Organizer
      Satellite Symposium of the 39th Annual Scientific Meeting of the Japan Atherosclerosis Society
    • Place of Presentation
      大阪
    • Year and Date
      2007-07-15
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Disruption of acl-4, an evolutionarily conserved acyltransferase-like gene, causes synergistic movement defects under polyuns2007

    • Author(s)
      Naoko Hara
    • Organizer
      The 5th Korea-Japan Conference on Cellular Signaling for Young Scientists
    • Place of Presentation
      Gyeongju, Korea
    • Year and Date
      2007-07-10
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Disruption of acl-4, an evolutionarily conserved acyltransferase-like gene, causes synergistic movement defects under polyunsaturated fatty acids depleted condition in C. elegans2007

    • Author(s)
      Naoko Hara
    • Organizer
      The 5th Korea-Japan Conference on Cellular Signaling for Young Scientists
    • Place of Presentation
      Gyeongju, Korea
    • Year and Date
      2007-07-10
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Disruption of acl-4, an evolutionarily conserved acyltransferase-like gene, causes synergistic movement defects under polyuns2007

    • Author(s)
      Naoko Hara
    • Organizer
      16th International C. elegans Meeting
    • Place of Presentation
      University of California, Los Angeles
    • Year and Date
      2007-06-30
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Functional analysis of acl-10, a novel acyltransferase in C. elegans2007

    • Author(s)
      Rieko Imae
    • Organizer
      16th International C. elegans meeting
    • Place of Presentation
      University of California, Los Angeles
    • Year and Date
      2007-06-30
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Disruption of acl-4, an evolutionarily conserved acyltransferase-like gene, causes synergistic movement defects under polyunsaturated fatty acids depleted condition in C. elegans2007

    • Author(s)
      Naoko Hara
    • Organizer
      16th International C. elegans Meeting
    • Place of Presentation
      University of California, Los Angeles, USA
    • Year and Date
      2007-06-30
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Functional analysis of acl-10, a novel acyltransferase in C. elegans2007

    • Author(s)
      Rieko Imae
    • Organizer
      16th International C. elegans Meeting
    • Place of Presentation
      University of California, Los Angeles, USA
    • Year and Date
      2007-06-30
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Cell Polarity during Terminal Asymmetric Cell Division of Stem Cell-like epithelia is regulated by Intracellular Phospholipas2007

    • Author(s)
      Takahiro Kanamori
    • Organizer
      16th International C. elegans Meeting
    • Place of Presentation
      University of California, Los Angeles
    • Year and Date
      2007-06-27
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Cell Polarity during Terminal Asymmetric Cell Division of Stem Cell-like epithelia is regulated by Intracellular Phospholipase A1 in C. elegans2007

    • Author(s)
      Takahiro Kanamori
    • Organizer
      16th International C. elegans Meeting
    • Place of Presentation
      University of California, Los Angeles, USA
    • Year and Date
      2007-06-27
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] 細胞内II型PAFアセチルハイドロラーゼ欠損マウスの解析2007

    • Author(s)
      河野望
    • Organizer
      第49回日本脂質生化学会
    • Place of Presentation
      札幌
    • Year and Date
      2007-06-06
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Essential role of intracellular type IIPAF-AH in epithelial cell formation2007

    • Author(s)
      Hiroyuki Arai
    • Organizer
      3rd INTERNATIONAL CONFERENCE ON PHOSPHOLIPASE A2 AND LIPID MEDIATORS
    • Place of Presentation
      Naples, Italy
    • Year and Date
      2007-05-11
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Analysis of type II PAF acetylhydrolase-deficient mice2007

    • Author(s)
      Nozomu Kono
    • Organizer
      3rd INTERNATIONAL CONFERENCE ON PHOSPHOLIPASE A2 AND LIPID MEDIATORS
    • Place of Presentation
      Naples, Italy
    • Year and Date
      2007-05-10
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] 線虫C.elegansを用いた高度不飽和脂肪酸の機能解析2007

    • Author(s)
      李賢哲
    • Organizer
      日本薬学会 第127年会
    • Place of Presentation
      富山
    • Year and Date
      2007-03-28
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] 生理活性脂質リゾホスファチジン酸による血管新生・細胞浸潤制御とその意義2007

    • Author(s)
      青木 淳賢
    • Organizer
      がん特定研究公開シンポジウム
    • Place of Presentation
      東京
    • Year and Date
      2007-02-22
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Physiological role of autotaxin in mouse development2006

    • Author(s)
      Masayuki Tanaka, Shinichi Okudaira, Yasuhiro Kishi, Junken Aoki, Hiroyuki Arai
    • Organizer
      5th Pharma Bioforum 2006
    • Place of Presentation
      Tokyo, Japan
    • Year and Date
      20061209-10
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Analysis of PS-PLA1 under inflammatory condition2006

    • Author(s)
      Naoya Saiki, Kumiko Makide, Junken Aoki, Hiroyuki Arai
    • Organizer
      5th Pharma Bioforum 2006
    • Place of Presentation
      Tokyo, Japan
    • Year and Date
      20061209-10
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Disruption of acl-4, an evolutionarily conserved acyltransferase-like gene, causes synergistic movement defects under polyunsaturated fatty acids depleted condition in C. elegans2006

    • Author(s)
      Naoko Hara, Takao Inoue, Hiroyuki Arai
    • Organizer
      5th Pharma Bioforum 2006
    • Place of Presentation
      Tokyo, Japan
    • Year and Date
      20061209-10
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Intracellular phospholipase A I is essential for controlling terminal differentiation of stem cell-like epithelia in C. elegans2006

    • Author(s)
      Takahiro Kanamori, Takao Inoue, Taro Sakamoto, Yasuhito Nakagawa, Masafumi Tsujimoto, Junken Aoki, Hiroyuki Arai
    • Organizer
      C. elegans Development&Evolution Topic Meeting#1
    • Place of Presentation
      University of Wisconsin-Madison
    • Year and Date
      20060622-25
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Intracellular phospholipase A12006

    • Author(s)
      Junken Aoki
    • Organizer
      20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress
    • Place of Presentation
      Kyoto, Japan
    • Year and Date
      20060618-23
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Enhancement of Implantation by a Novel Lysophosphatidic Acid-Producing Enzyme in vivo2006

    • Author(s)
      Asuka Inoue, Kotaro Hama, Junken Aoki, Hiroyuki Arai
    • Organizer
      20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress
    • Place of Presentation
      Kyoto, Japan
    • Year and Date
      20060618-23
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Physiological role of autotaxin in mouse development2006

    • Author(s)
      Masayuki Tanaka, Shinichi Okudaira, Yasuhiro Kishi, Ryunosuke Ohkawa, Sachiko Iseki, Masato Ota, Sumihare Noji, Yutaka Yatomi, Junken Aoki, Hiroyuki Arai
    • Organizer
      20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress
    • Place of Presentation
      Kyoto, Japan
    • Year and Date
      20060618-23
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Analysis of Oxysterol binding protein (OSBP)-related genes in C. elegans2006

    • Author(s)
      Hiroyuki Kobuna, Takao Inoue, Hiroyuki Arai
    • Organizer
      20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress
    • Place of Presentation
      Kyoto, Japan
    • Year and Date
      20060618-23
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Intracellular Phospholipase A1 is required for epithelial tissue integrity in Caenorhbditis elegans2006

    • Author(s)
      Takahiro Kanamori, Junken Aoki, Takao Inoue, Taro Sakamoto, Masaki Ishikawa, Ryo Taguchi, Yasuhito Nakagawa, Masafumi Tsujimoto, Hiroyuki Arai
    • Organizer
      20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress
    • Place of Presentation
      Kyoto, Japan
    • Year and Date
      20060618-23
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Acl-10, an evolutionarily conserved acyltransferase, regulates muscle contraction in C. elegans2006

    • Author(s)
      Rieko Imae, Takao Inoue, Naoko Hara, Yasukazu Takanezawa, Keiko Gengyo-Ando, Shohei Mitani, Hiroyuki Arai
    • Organizer
      20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress
    • Place of Presentation
      Kyoto, Japan
    • Year and Date
      20060618-23
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Structure and function of a novel phospholipase Al family2006

    • Author(s)
      Junken Aoki
    • Organizer
      3rd Lipidomics Meeting "From Lipid Analysis to Genetic Disorders
    • Place of Presentation
      Marseille, France
    • Year and Date
      20060511-13
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] リゾホスファチジン酸産生酵素オートタキシンの胎生期血管形成への関与2006

    • Author(s)
      田中将之
    • Organizer
      第5回ファーマ・バイオフォーラム2006
    • Place of Presentation
      東京
    • Year and Date
      2006-12-09
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] 炎症刺激によるホスファチジルセリン特異的ホスホリパーゼA1(PS-PLA1)の発現誘導2006

    • Author(s)
      斉木直矢
    • Organizer
      第5回ファーマ・バイオフォーラム2006
    • Place of Presentation
      東京
    • Year and Date
      2006-12-09
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] 線虫C.elegansの運動機能に関与するアシルトランスフェラーゼ様遺伝子acl-4の機能解析2006

    • Author(s)
      原直子
    • Organizer
      第5回ファーマ・バイオフォーラム2006
    • Place of Presentation
      東京
    • Year and Date
      2006-12-09
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Intracellular Phospholipase A1 is Essential for Terminal Asymmetric Cell Division of Stem Cell-like Epithelia in C. elegans2006

    • Author(s)
      Kanamori T.
    • Organizer
      日本分子生物学会2006フォーラム
    • Place of Presentation
      名古屋
    • Year and Date
      2006-12-06
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] 線虫C. elegansを用いた新規アシルトラシスフェラーゼ様遺伝子acl-10の機能解析2006

    • Author(s)
      今江 理恵子
    • Organizer
      日本分子生物学会2006フォーラム
    • Place of Presentation
      名古屋
    • Year and Date
      2006-12-06
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] 細胞内型ホスホリパーゼA1 KIAA0725はリゾホスファチジルエタノールアミンを産生し、ER-ゴルジ間の逆行輸送に関与する2006

    • Author(s)
      森川 麗
    • Organizer
      日本分子生物学会2006フォーラム
    • Place of Presentation
      名古屋
    • Year and Date
      2006-12-06
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] 細胞内型ホスホリパーゼA1 KIAA0725はリゾボスファチジルエタノールアミンを産生し、ER-ゴルジ間の逆行輸送に関与する2006

    • Author(s)
      森川 麗
    • Organizer
      東京大学生命科学研究ネットワークシンポジウム
    • Place of Presentation
      東京
    • Year and Date
      2006-11-25
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] 酸化リン脂質分解酵素II型PAF-AHノックアウトマウスの解析2006

    • Author(s)
      河野 望
    • Organizer
      第28回生体膜と薬物の相互作用シンポジウム
    • Place of Presentation
      静岡
    • Year and Date
      2006-11-09
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] マスト細胞lysoPS受容体の構造活性相関2006

    • Author(s)
      斉木直矢
    • Organizer
      フオーラム2006衛生薬学・環境トキシコロジー
    • Place of Presentation
      東京
    • Year and Date
      2006-10-30
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] リゾリン脂質によるアレルギー反応の調節2006

    • Author(s)
      巻出久美子
    • Organizer
      フォーラム2006衛生薬学・環境トキシコロジー
    • Place of Presentation
      東京
    • Year and Date
      2006-10-30
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] 生理活性脂質リゾホスファチジン酸産生酵素オートタキシンの機能解析2006

    • Author(s)
      青木 淳賢
    • Organizer
      群馬大学21世紀COE若手シンポジウム
    • Place of Presentation
      群馬
    • Year and Date
      2006-10-04
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] シナプス伝達に関与するアシルトランスフェラーゼ様遺伝子acl-4の解析2006

    • Author(s)
      原直子
    • Organizer
      第29回 日本神経科学大会
    • Place of Presentation
      京都
    • Year and Date
      2006-07-19
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Disruption of acl-4, an evolutionarily conserved acyltransferase-like gene, causes synergistic movement defects under polyunsaturated fatty acids depleted condition in C. elegans2006

    • Author(s)
      Naoko Hara
    • Organizer
      C. elegans Neuroscience Meeting
    • Place of Presentation
      University of Wisconsin-Madison
    • Year and Date
      2006-07-09
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Intracellular phospholipase A1 is essential for controlling terminal differentiation of stem cell-like epithelia in C. elegans2006

    • Author(s)
      Takahiro Kanamori
    • Organizer
      C. elegans Development & Evolution Topic Meeting#1
    • Place of Presentation
      University of Wisconsin-Madison
    • Year and Date
      2006-06-22
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Intracellular phospholipase A12006

    • Author(s)
      Junken Aoki
    • Organizer
      20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress
    • Place of Presentation
      Kyoto
    • Year and Date
      2006-06-18
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Enhancement of Implantation by a Novel Lysophosphatidic Acid-Producing Enzy me in vivo2006

    • Author(s)
      Asuka Inoue
    • Organizer
      20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress
    • Place of Presentation
      Kyoto
    • Year and Date
      2006-06-18
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Physiological role of autotaxin in mouse development2006

    • Author(s)
      Masayuki Tanaka
    • Organizer
      20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress
    • Place of Presentation
      Kyoto
    • Year and Date
      2006-06-18
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Intracellular Phospholipase A1 is required for epithelial tissue integrity in Caenorhbditis elegans2006

    • Author(s)
      Takahiro Kanamori
    • Organizer
      20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress
    • Place of Presentation
      Kyoto
    • Year and Date
      2006-06-18
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Acl-10, an evolutionarily conserved acyltransferase, regulates muscle contraction in C. elegans2006

    • Author(s)
      Rieko Imae
    • Organizer
      20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress
    • Place of Presentation
      Kyoto
    • Year and Date
      2006-06-18
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] 細胞内小胞輸送に関与する新規PIPs結合タンパク質2006

    • Author(s)
      長谷川 純矢
    • Organizer
      第48回 脂質生化学会
    • Place of Presentation
      東京
    • Year and Date
      2006-06-08
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] 新規ホスファチジン酸特異的ホスホリパーゼA1の着床促進効果2006

    • Author(s)
      井上飛鳥
    • Organizer
      第48回 脂質生化学会
    • Place of Presentation
      東京
    • Year and Date
      2006-06-08
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] リゾホスファチジルセリン(lysoPS)誘導体を用いたマスト細胞上のlysoPS受容体の解析2006

    • Author(s)
      巻出久美子
    • Organizer
      第48回 脂質生化学会
    • Place of Presentation
      東京
    • Year and Date
      2006-06-08
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Structure and function of a novel phospholipase A1 family2006

    • Author(s)
      Junken Aoki
    • Organizer
      3rd Lipidomics Meeting "From Lipid Analysis to Genetic Disorders"
    • Place of Presentation
      Marseille, France
    • Year and Date
      2006-05-11
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] リゾホスファチジルセリン(lysoPS)誘導体を用いたマスト細胞lysoPS受容体の性状解析2006

    • Author(s)
      青木 淳賢
    • Organizer
      日本ケミカルバイオロジー研究会
    • Place of Presentation
      東京
    • Year and Date
      2006-05-10
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] ラット腹腔マスト細胞に対するモノクローナル抗体の作製2005

    • Author(s)
      巻出久美子
    • Organizer
      日本生化学会
    • Place of Presentation
      神戸
    • Year and Date
      20051000
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] マウスの発生におけるオートタキシンの生理的役割2005

    • Author(s)
      田中将之
    • Organizer
      日本生化学会
    • Place of Presentation
      神戸
    • Year and Date
      20051000
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] 細胞内II型PAF-AH欠損マウスの作製と脂質解析2005

    • Author(s)
      河野 望
    • Organizer
      日本生化学会
    • Place of Presentation
      神戸
    • Year and Date
      20051000
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] 血清中のリゾホスホリパーゼD活性およびリゾホスファチジン酸産生におけるオートタキシンの寄与2005

    • Author(s)
      奥平真一
    • Organizer
      日本生化学会
    • Place of Presentation
      神戸
    • Year and Date
      20051000
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] リパーゼによる不飽和リゾホスファチジルコリン産生とリゾホスホリパーゼDによるLPA代謝機構の解析2005

    • Author(s)
      岸安宏
    • Organizer
      日本生化学会
    • Place of Presentation
      神戸
    • Year and Date
      20051000
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] 新規LPA3選択的アゴニストT13の解析2005

    • Author(s)
      濱弘太郎
    • Organizer
      日本生化学会
    • Place of Presentation
      神戸
    • Year and Date
      20051000
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] I型PAFアセチルハイドロラーゼ活性サブユニットと滑脳症原因遺伝子LIS1との相互作用機構の解析2005

    • Author(s)
      立澤京
    • Organizer
      日本生化学会
    • Place of Presentation
      神戸
    • Year and Date
      20051000
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] マウス腎臓におけるDHA含有リン脂質がエンドトキシンにより変化を起こす2005

    • Author(s)
      高根沢康一
    • Organizer
      日本生化学会
    • Place of Presentation
      神戸
    • Year and Date
      20051000
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] マウス脳における新規コリン特異的グリセロホスホジエステルーホスホジエステラーゼNPP6の生理機能解析2005

    • Author(s)
      坂上秀樹
    • Organizer
      日本生化学会
    • Place of Presentation
      神戸
    • Year and Date
      20051000
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] C. elegansを用いた細胞内ホスホリパーゼA1の機能解析2005

    • Author(s)
      金森崇浩
    • Organizer
      日本生化学会
    • Place of Presentation
      神戸
    • Year and Date
      20051000
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] 細胞内ホスホリパーゼA1、KIAA0725の機能解析2005

    • Author(s)
      森川麗
    • Organizer
      日本生化学会
    • Place of Presentation
      神戸
    • Year and Date
      20051000
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] C. elegansを用いたアシルトランスフェラーゼの機能解析2005

    • Author(s)
      今江理恵子
    • Organizer
      日本生化学会
    • Place of Presentation
      神戸
    • Year and Date
      20051000
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] C. elegansの運動機能に関与する新規アシルトランスフェラーゼ様遺伝子acl-4の機能解析2005

    • Author(s)
      原直子
    • Organizer
      日本生化学会
    • Place of Presentation
      神戸
    • Year and Date
      20051000
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] 着床におけるリゾホスファチジン酸産生機構の解析2005

    • Author(s)
      井上 飛鳥
    • Organizer
      日本生化学会
    • Place of Presentation
      神戸
    • Year and Date
      20051000
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] リゾホスファチジルセリンと即時型アレルギー2005

    • Author(s)
      青木淳賢
    • Organizer
      6th Pharmaco-Hematology Symposium
    • Place of Presentation
      東京
    • Year and Date
      20050700
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] PDZK1 C-ter;minal region, not directly involved in SR-BI binding, is required for up-regulation of SR-BI expression.2005

    • Author(s)
      Toshiyuki Nakamura
    • Organizer
      2nd International Symposium on Triglycerides and HDL
    • Place of Presentation
      New York U.S.A
    • Year and Date
      20050700
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] PDZK1 C-ter;minal region, not directly involved in SR-BI binding, is required for up-regulation of SR-BI expresion2005

    • Author(s)
      Toshiyuki Nakamura
    • Organizer
      2nd International Symposium on Triglycerides and HDL
    • Place of Presentation
      New York U.S.A.
    • Year and Date
      20050700
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] C.elegansを用いた細胞内ホスホリパーゼA1の機能解析2005

    • Author(s)
      金森崇浩
    • Organizer
      日本脂質生化学会
    • Place of Presentation
      金沢
    • Year and Date
      20050600
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Biochemical and Molecular Characterization of a Novel Choline-specific Glycerophosphodiester Phosphodiesterase Belonging to the Nucleotide Pyrophosphatase/Phosphodiesterase (NPP) family2005

    • Author(s)
      Hideki Sakagami
    • Organizer
      FASEB Summer Research Conferences : Lysophospholipid Mediators in Health and Disease
    • Place of Presentation
      Snowmass Village, Colorado
    • Year and Date
      20050600
    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2010-02-04  

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