2007 Fiscal Year Final Research Report Summary
Improved breeding for disease resistant strain of fugu following whole genome sequencing
| Project/Area Number |
17208017
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General fisheries
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| Research Institution | The University of Tokyo |
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Principal Investigator |
SUZUKI Yuzuru The University of Tokyo, Graduate School of Agricultural and Life Sciences, Professor (40107412)
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| Co-Investigator(Kenkyū-buntansha) |
KIKUCHI Kiyoshi The University of Tokyo, Graduate School of Agricultural and Life Sciences, Assistant Professor (20292790)
SUETAKE Hiroaki The University of Tokyo, Graduate School of Agricultural and Life Sciences, Assistant Professor (00334326)
OGAWA Kazuo The University of Tokyo, Graduate School of Agricultural and Life Sciences, Professor (20092174)
MIYADAI Toshiaki Fukui Prefectural University, Faculty of Marine Bioscience, Professor (20157663)
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| Project Period (FY) |
2005 – 2007
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| Keywords | Fugu / Genome / Breeding / Parasite / Disease resistance / Immunity / Genomic linkage map / Heterobothrium okamotoi |
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| Research Abstract |
The fugu Takifugu rubripes is the only economic species among the fishes with genomes that have been sequenced. The aim of this study was to establish the strategy for the improved breeding for disease resistant strain of this species utilizing genome sequences.
1. Search for immune factors
Using homology searches, we succeeded to find many genes which are related to the immune reaction, such as B-cell and T-cell related factors, cytokines, chemokines, complement factors, and lectins. We are now able to make further investigations of immune response against the parasite, since this fish has a large body size that enables us to analyze proteins as well as cells related to immunity.
2. Genetic linkage map.
Since the fugu genome database is composed of highly fragmented sequences, we needed to make a genetic linkage map for the genetic research of this species. We have constructed the linkage map containing 1219microsatellite loci that provide anchor points to the 86% of the genome assembly.
3. QTL analyses for resistance/susceptibility against a parasite, Heterobothrium okamotoi.
One of the biggest problems for fugu culture is a parasite Heterobothrium okamoti, which cause anaemia by taking blood at the gill. Establishment of the linkage map enabled us to conduct QTL analysis of molecules related to disease resistance, although strains of this species are quite limited. We used inter species hybrid with Kusa-fugu, T. niphobles, which is resistant against the parasite. F2 generation showed wide individual variations in the resistance/susceptibility against the parasite. By QTL analysis, we found each two loci of parasite number and parasite growth. Further extensive research will lead to determination of the genes.
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Research Products
(73 results)
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[Journal Article] Gene organization and expression of CD8α and CD8α genes in fugu Takifugu rubripes.2007
Author(s)
Suetake, H., Araki, K., Akatsu, K., Somamoto, T., Dijkstra. J. M., Yoshiura, Y., Kikuchi, K., Suzuki. Y.
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Journal Title
Fish Shellfish Immunol. 23
Pages: 1107-1118
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Description of a fugu CXC chemokine and two CXC receptor genes, and characterization of the effects of different stimulators on their expression.2007
Author(s)
Saha, N. R., Bei, J-X., Suetake, H., Araki, K., Kai, W., Kikuchi, K., Lin, H-R., Suzuki, Y.
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Journal Title
Fish Shellfish Immunol. 23
Pages: 1324-1332
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] The sex-determining locus in the tiger pufferfish, Takifugu rubripes.2007
Author(s)
Kikuchi, K., Kai, W., Hosokawa, A., Mizuno, N., Suetake, H., Asahina, K., Suzuki, Y.
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Journal Title
Genetics 175
Pages: 2039-2042
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Gene organization and expression of CD8α and CD8α genes in fugu Takifugu ruhripes.2007
Author(s)
Suetake, H., Araki, K., Akatsu, K., Somamoto, T., Dijkstra, J. M., Yoshimura, Y., Kikuchi, K., Suzuki, Y.
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Journal Title
Fish Shellfish Immunology 23
Pages: 1107-1118
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Description of a fugu CXC chemokine and two CXC receptor genes, and characterization of the effects of different stimulators on their expression.2007
Author(s)
Saha, N. R., Bei, J-X., Suetake, H., Araki, K., Kai, W., Kikuchi, K., Lin, H-R., Suzuki, Y.
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Journal Title
Fish Shellfish Immunol 23
Pages: 1324-1332
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Possible role of a skin mucus lectin in fugu (Takifugu rubripes) in excluding marine bacteria from the body surface to environmental water.2006
Author(s)
Tsutsui, S., Nishikawa, E., Mano, N., Hirose, H., Tasumi, S., Suetake, H., Suzuki, Y.
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Journal Title
Fisheries Sci. 72
Pages: 455-457
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Two Interleukin (IL) -15 homologs from two distinct origins in fish.2006
Author(s)
Bei, J-X., Suetake, H., Araki, K., Kikuchi, K., Yoshiura, Y., Lin, H-R., Suzuki, Y.
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Journal Title
Mol. Immunol. 43
Pages: 860-869
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Lymphocyte surface marker genes in fugu.2006
Author(s)
Suetake, H., Saha, N. R., Arakia, K., Akatsu, K., Kikuchi, K., Suzuki, Y.
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Journal Title
Comp. Biochem. Physiol. 1D
Pages: 102-108
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Novel Mannose-Specific Lectins Found in Fugu, Takifugu rubripes ; A Review.2006
Author(s)
Tsutsui. S., Okamoto, M., Tasumil, S., Suetake, H., Kikuchi, K., Suzuki. Y.
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Journal Title
Comp. Biochem. Physiol. ID
Pages: 122-127
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Possible role of a skin mucus lectin in fugu (Tnkifugu rubripes) in excluding marine bacteria from the body surface to environmental water.2006
Author(s)
Tsutsui, S., Nishikawa, E., Mano, N., Hirose, H., Tasumi, S., Suetake, H., Suzuki, Y.
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Journal Title
Fisheries Sci. 72
Pages: 455-457
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Two Interleukin (TL) -15 homologs from two distinct origins in fish.2006
Author(s)
Bei, J-X., Suetake, H., Araki, K., Kikuchi, K., Yoshiura, Y., Lin, H-R., Suzuki, Y.
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Journal Title
Mol. Immunol. 43
Pages: 860-869
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Novel Mannose-Specific Lectins Found in Fugu, Tnkifugu rubripes, A Review.2006
Author(s)
Tsutsui, S., Okamoto, M., Tasumil, S., Suetake, H., Kikuchi, K., Suzuki, Y
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Journal Title
Comp. Biochfm Physiol. 1D
Pages: 122-127
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] A genetic linkage map for the tiger pufferfish, takifugu rubripes2005
Author(s)
Kai, W., Kikuchi, K., Fujita, M., Suetake, H., Yoshiura, Y., Ototake, M., Fujiwara, A., Venkatesh, B., Miyaki, K., Suzuki, Y.
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Journal Title
Geneties 171
Pages: 227-238
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] A genetic linkage map for the tiger pufferfish, Tnkifugu rubripes2005
Author(s)
Kai, W., Kikuchi, K., Fujita, M., Suetake, H., Yoshiura, Y., Ototake, M., Fujiwara, A., Venkatesh, B., Miyaki, K., Suzuki, Y
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Journal Title
Genetics 171
Pages: 227-238
Description
「研究成果報告書概要(欧文)」より
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[Presentation] トラフグIL-22006
Author(s)
三浦, ら
Organizer
平成18年度日本水産学会大会
Place of Presentation
高知
Year and Date
20060300
Description
「研究成果報告書概要(和文)」より
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