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2008 Fiscal Year Final Research Report

Molecular mechanism of sex determination in fish

Research Project

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Project/Area Number 17208019
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field General fisheries
Research InstitutionUniversity of the Ryukyus

Principal Investigator

NAKAMURA Masaru  University of the Ryukyus, 熱帯生物圏研究センター, 教授 (10101734)

Co-Investigator(Kenkyū-buntansha) KOBAYASHI Tohru  愛媛大学, 南予水産研究センター, 准教授 (30221972)
HIRAI Toshiaki  帝京科学大学, 理工学部, 講師 (30238331)
Project Period (FY) 2005 – 2008
Keywords性分化 / 性転換 / 性決定 / 雌性ホルモン / 雄性ホルモン / アロマターゼ / ステロイド代謝酵素 / 生殖細胞
Research Abstract

魚類の性決定機構の解明を組織学的、免疫組織学的、細胞学的及び分子生物学的手法を用いて行った。その結果、雌雄異体魚の成熟卵巣を精巣へと転換させることに成功した。性決定に雌性ホルモンと雄性ホルモンのバランスの変化が重要な役割を果たしていることが明らかにした。更に、性決定には脳からの刺激である生殖腺刺激ホルモン及び生殖腺内ではそのシグナルを受け取る生殖腺刺激ホルモン受容体が重要であることを明らかにした。

  • Research Products

    (61 results)

All 2009 2008 2007 2006 2005

All Journal Article (48 results) (of which Peer Reviewed: 48 results) Presentation (10 results) Book (3 results)

  • [Journal Article] Immunohistochemical evidence for 11 β-hydroxylase(P45011 β)and androgen production in the gonad during sex differentiation and in adults in the protandrous anemonefish Amphiprion clarkii2008

    • Author(s)
      S. Miura, R. Horiguchi, M. Nakamura
    • Journal Title

      Zool Sci 25

      Pages: 212-219

    • Peer Reviewed
  • [Journal Article] Sexual dimorphic expression of genes in gonads during early differentiation of a Teleost fish, the nile tilapia2008

    • Author(s)
      S. Ijiri, T. Kobayashi, M. Nakamura(他5人)
    • Journal Title

      Oreochromis niloticus.Biology of reproduction 78

      Pages: 333-341

    • Peer Reviewed
  • [Journal Article] Sex change of adult initial-phase male wrasse, Halichoeres trimaculatus by estradiol-17β treatment2008

    • Author(s)
      Y. Kojima, R.K. Bhandari, T. Kobayashi, M. Nakamura
    • Journal Title

      Gen Comp Endocrinol 156

      Pages: 628-632

    • Peer Reviewed
  • [Journal Article] Appearances and chronological changes of mummichog Fundulus heteroclitus FSH cells and LH cells curing ontogeny, sexual differentiation, and gonadal development2008

    • Author(s)
      A. Shimizu, T. Kobayashi, M. Nakamura(他5人)
    • Journal Title

      Gen Comp Endocrinol 156

      Pages: 312-322

    • Peer Reviewed
  • [Journal Article] Differentiation of ambisexual gonads and immunohistochemical localization of P450 cholesterol side-chain cleavage enzyme during gonadal sex differentiation in the protandous anemonefish2008

    • Author(s)
      S. Miura, S. Nakamura, Y. Kobayashi, P. Francesc, M. Nakamura
    • Journal Title

      Amphiprion clarkii. Comp. Biochem. Physiol B149

      Pages: 29-37

    • Peer Reviewed
  • [Journal Article] Molecular cloning and expression of three estrogen receptors in the protogynous wrasse2008

    • Author(s)
      R. Horiguchi, Y. Kobayashi and M. Nakamura
    • Journal Title

      Halichoeres trimaculatus. Cybium 32(2)

      Pages: 88-99

    • Peer Reviewed
  • [Journal Article] In vitro induction of gonadal sex change in a protogynous fish, three-spotted wrasse2008

    • Author(s)
      T. Todo, M. Nakamura(他6人)
    • Journal Title

      (Halichores trimaculatus). Cybium 32(2)

      Pages: 106

    • Peer Reviewed
  • [Journal Article] Post-sex differentiational' sex reversal in the female common carp2008

    • Author(s)
      S. Ogawa, M. Akiyoshi, M. Higuchi, M. Nakamura, T. Hirai
    • Journal Title

      (Cyprinus carpio). Cybium 32(2)

      Pages: 102-103

    • Peer Reviewed
  • [Journal Article] The role of estrogen in gonadal sex differentiation in protandrous anemonefish Amphiprion clarkii2008

    • Author(s)
      S. Miura, M. Nakamura
    • Journal Title

      Immunohistochemical localization of P450scc in gonads of male and female phases. Cybium 32(2)

      Pages: 87

    • Peer Reviewed
  • [Journal Article] Molecular cloning and quantitative expression of sexually dimorphic markers Dmrt1 and Foxl2 during female-to-male sex change in Epinephelus merra2008

    • Author(s)
      M .A. Alam, Y. Kobayashi, R. Horiguchi, T. Hirai, M. Nakamura
    • Journal Title

      Gen Comp Endocrinol 157

      Pages: 75-85

    • Peer Reviewed
  • [Journal Article] Determination of sex and gonadal maturity in the honeycomb grouper2008

    • Author(s)
      M.A. Alam, M. Nakamura
    • Journal Title

      Epinephelus merra, through biopsy. Aquacult. Int 16

      Pages: 27-32

    • Peer Reviewed
  • [Journal Article] Phylogenetic diversity and co-symbiosis in the bioluminescent symbioses of Photobacterium mandapamenis2008

    • Author(s)
      A.J. Kaeding, M. Nakamura 他6人
    • Journal Title

      Appl Environ Microbiol

      Pages: 3173-3182

    • Peer Reviewed
  • [Journal Article] Stability in aromatase immunoreactivity of steroid-producing cells during early Development of XX gonads of the Nile tilapia2008

    • Author(s)
      F. Sakai, T. Kobayashi, M. Matsuda and Y.Nagahama
    • Journal Title

      Oreochromis niloticus : An organ culture Study. Zool. Sci 25(3)

      Pages: 344-348

    • Peer Reviewed
  • [Journal Article] Sexual dimorphic expression of DMRT1 and Sox9 during gonadal differentiation and hormone-induced sex reversal in the teleost fish Nile tilapia(Oreochromis niloticus)2008

    • Author(s)
      T. Kobayashi, H. Kajiura-Kobayashi, G. Guan and Y. Nagahama
    • Journal Title

      Dev. Dyn 237(1)

      Pages: 297-306

    • Peer Reviewed
  • [Journal Article] Molecular Cloning of Plk1 and Nek2 and their Expression in Mature Gonads of the Teleost Fish Nile Tilapia(Oreochromis Niloticus)2008

    • Author(s)
      Y. Matsuoka, T. Kobayashi, K. Kihara and Y. Nagahama
    • Journal Title

      Mol. Reprod. Develop 75(6)

      Pages: 989-1001

    • Peer Reviewed
  • [Journal Article] Seabream GnRH immunoreactivity in brain and pituitary of XX and XY Nile tilapia,2008

    • Author(s)
      I. Swapna, T. Kobayashi(他6人)
    • Journal Title

      Oreochroomis niloticus during early development. J. Exp. Zool., Part A 309(7)

      Pages: 419-426

    • Peer Reviewed
  • [Journal Article] Molecular cloning and gene expression of Foxl2 in the frog Rana rugosa. Gen2008

    • Author(s)
      Y. Oshima, Y. Uno, Y. Matsuda, T. Kobayashi and M. Nakamura
    • Journal Title

      Comp. Endocrinol 159(2-3)

      Pages: 170-177

    • Peer Reviewed
  • [Journal Article] Transgenic medaka enables easy detection of oocytes in live fish2008

    • Author(s)
      M. Kinoshita, T. Kobayashi(他6人)
    • Journal Title

      Mol. Reprod. Develop 76(2)

      Pages: 202-207

    • Peer Reviewed
  • [Journal Article] 沖縄の魚の性転換2007

    • Author(s)
      中村將
    • Journal Title

      日本病態生理学会雑誌 16

      Pages: 25-281

    • Peer Reviewed
  • [Journal Article] Nakamura, Efferent duct differentiation during female-tomale sex change in honeycomb grouper Epinephelus merra2007

    • Author(s)
      Alam M.A. and M. Nakamura
    • Journal Title

      J Fish Biol 71

      Pages: 1-11

    • Peer Reviewed
  • [Journal Article] Role of sex hormones in sex change of grouper2007

    • Author(s)
      M. Nakamura, M.A. Alam, Y. Kobayashi, R.K. Bhandari
    • Journal Title

      Journal of Marine Science and Technology, Special Issue

      Pages: 23-27

    • Peer Reviewed
  • [Journal Article] Phylogenetic diversity and co-symbiosis in the Bioluminescent Symbioses of Photobacterium mandapamensis Appl2007

    • Author(s)
      J.K. Alison, M. Nakamura(他6人)
    • Journal Title

      Environ. Microbiol : 10.1128/AEM

      Pages: 02212-06

    • Peer Reviewed
  • [Journal Article] Foxl2 up-regulates aromatase gene transcription female-specifically by binding to the promoter as well as interacting with Ad4BP/SF-12007

    • Author(s)
      D.S. Wang, T. Kobayashi, L.Y. Zhou, B. Paul-Prasanth, S. Ijiri, F. Sakai, K. Okubo, K.I. Morohashi and Y. Nagahama.
    • Journal Title

      Mol. Endocrinol 21(3)

      Pages: 712-725

    • Peer Reviewed
  • [Journal Article] DMY induces male development in XX medaka fish. Proc. Natl2007

    • Author(s)
      M. Matsuda, T. Kobayashi(他8人)
    • Journal Title

      Natl. Acad. Sci. USA 104(10)

      Pages: 3865-3870

    • Peer Reviewed
  • [Journal Article] A novel type of p450c17 lacking the lyase activity is responsible for c21-steroid biosynthesis in the fish ovary and head kidney2007

    • Author(s)
      L.Y. Zhou, T. Kobayashi(他6人)
    • Journal Title

      Endocrinology 148(9)

      Pages: 4282-4291

    • Peer Reviewed
  • [Journal Article] Suppression of steroidogenic enzyme expression during androgen-induced sex reversal in Nile tilapia(Oreochromis niloticus)2006

    • Author(s)
      K.B. Ramji, M. Nakamura, T. Kobayashi, and Y. Nagahama
    • Journal Title

      Gen Comp Endocrinol 145

      Pages: 20-24

    • Peer Reviewed
  • [Journal Article] Sex change in coral reef fish2006

    • Author(s)
      M. Nakamura, Y. Kobayashi, S. Miura, M.A. Alam, K.B. Ramji
    • Journal Title

      Fish Physiol Biochem 31

      Pages: 117-122

    • Peer Reviewed
  • [Journal Article] Changes in androgen-producing cell size and circulation 11-ketotestoterone level during famal-male sex change of honycomb grouper epinephelus merra2006

    • Author(s)
      M.A. Alam, R.K. Bhandari, Y. Kobayashi, S. Nakamura, K. Soyano and M. Nakamura
    • Journal Title

      73

      Pages: 206-214

    • Peer Reviewed
  • [Journal Article] Induction of sex change within two full moons during breeding season and spawning in grouper2006

    • Author(s)
      M.A. Alam, R.K. Bhandari, Y. Kobayashi, K. Soyano, M. Nakamura
    • Journal Title

      Aquaculture 255

      Pages: 532-535

    • Peer Reviewed
  • [Journal Article] GnRHa-accelerated spermatogenesiss in the testes of underyearling golden rabbitfish2006

    • Author(s)
      T. Komatsu, R.K. Bhandari, Y. Kobayashi, S. Nakamura, M. Nakamura
    • Journal Title

      Siganus guttatus(Bloch). Aquaculture 257

      Pages: 558-565

    • Peer Reviewed
  • [Journal Article] A sex cord-like structure and some remarkable features in early gonadal sex differentiation in the marine teleost2006

    • Author(s)
      T. Komatsu, S. Nakamura and M. Nakamura
    • Journal Title

      Siganus guttatus(Bloch). J Fish Biol 68

      Pages: 236-250

    • Peer Reviewed
  • [Journal Article] Induction of female-to-male sex change in the Honeycomb grouper(Epinephelus merra)by 11-ketotestosterone treatments2006

    • Author(s)
      Ramji, K.B., Alam. M.A., K. Soyano and M. Nakamura
    • Journal Title

      Zoological Science 23

      Pages: 65-69

    • Peer Reviewed
  • [Journal Article] Knock-down of DMY initiates female pathway in the genetic male medaka2006

    • Author(s)
      B. Paul-Prasanth, M. Matsuda, E-L. Lau, A. Suzuki, F. Sakai, T. Kobayashi, and Y. Nagahama
    • Journal Title

      Oryzias latipes. Biochem. Biophys. Res. Commun 351(4)

      Pages: 815-519

    • Peer Reviewed
  • [Journal Article] サンゴ礁魚類の性分化、性転換機構の形態学的、生理学的研究2006

    • Author(s)
      中村將・小林靖尚・三浦さおり
    • Journal Title

      Morphological and physiologyical studies on sex differentiation and sex change in coralreef fish.(水産総合研究センター研究報告別冊4号別刷)

    • Peer Reviewed
  • [Journal Article] Promoter analysis of two aromatase genes in the serial-sex changing gobiid fish2005

    • Author(s)
      Y. Kobayashi, T. Sunobe, T. Kobayashi, Y. Nagahama, M. Nakamura
    • Journal Title

      Trimma Okinawae. Fish Physiol Biochem 31

      Pages: 123-127

    • Peer Reviewed
  • [Journal Article] Changes in serum steroid hormones during ovarian sevelopment in the captive common Japanese Conger Conger myriaster(Brevoort)2005

    • Author(s)
      H. Chiba, S. Ijiri, M. Iwata, M. Nakamura, S. Adachi and K. Yamauchi
    • Journal Title

      Aquaculture Science 53(2)

      Pages: 189-198

    • Peer Reviewed
  • [Journal Article] Aromatase immunoreactivity and the role of enzymes in steroid pathways for inducing sex change in the hermaphrodite gobiid fish Trimma Okinawae2005

    • Author(s)
      T. Sunobe, M. Nakamura, Y. Kobayashi, T. Kobayashi and Y. Nagahama
    • Journal Title

      Comp. Biochem. Physiol. Part A 141

      Pages: 54-59

    • Peer Reviewed
  • [Journal Article] Immunohistochemical evidence possibly identifying the site of androgen production in the ovary of the protogynous grouper Epinephelus merra2005

    • Author(s)
      M.A. Alam, H. Komuro, R.K. Bhandari, S. Nakamura, K. Soyano, M. Nakamura
    • Journal Title

      Cell Tissue Res 320

      Pages: 323-329

    • Peer Reviewed
  • [Journal Article] Gonadal structure and P450scc and 3β-HSD-like immunoreactivity in the gobiid fish Trimma okinawae during bidirectional sex change2005

    • Author(s)
      T. Sunobe, M. Nakamura, Y. Kobayashi, T. Kobayashi and Y. Nagahama
    • Journal Title

      Ichyiol Res 52

      Pages: 27-32

    • Peer Reviewed
  • [Journal Article] Gonadal structure of the serial-sex changing fish Trimma okinawae2005

    • Author(s)
      Kobayashi Y, Sunobe T, Kobayashi T, Nagahama Y and M Nakamura
    • Journal Title

      Dev Grouth Differ 47

      Pages: 7-13

    • Peer Reviewed
  • [Journal Article] Evidence that estrogen regulates the sex change of honeycomb grouper(Epinephelus merra), a protogynous hermaphrodite fish2005

    • Author(s)
      R.K. Bhandari, M.A. Alam, M. Higa, K. Soyano and M. Nakamura
    • Journal Title

      J Exp Zool 303A

      Pages: 497-503

    • Peer Reviewed
  • [Journal Article] Molecular cloning and expression of Ad4BP/SF-1 in the serial sex changing goiid fish Trimma okinawae2005

    • Author(s)
      Y. Kobayashi, T. Sunobe, T. Kobayashi, M. Nakamura, N. Suzuki and Y. Nagahama
    • Journal Title

      BBRC 332

      Pages: 1073-1080

    • Peer Reviewed
  • [Journal Article] Cloning of cDNAs and the differential expression of A-type cyclins and Dmc1 durinspermatogenesis in the Japanese eel, a teleost fish2005

    • Author(s)
      H. Kajiura-Kobayashi, T. Kobayashi, and Y. Nagahama
    • Journal Title

      Dev. Dyn 232(4)

      Pages: 1115-1123

    • Peer Reviewed
  • [Journal Article] Two types of aromatase with different encoding genes, tissue distribution and developmental Gen2005

    • Author(s)
      X. Chang, T. Kobayashi(他4人)
    • Journal Title

      Comp. Endocrinol 141(2)

      Pages: 101-115

    • Peer Reviewed
  • [Journal Article] Cloning, expression and characterization of three types of 17b-hydroxysteroid dehydrogenases from the Nile tilapia, Oreochromis niloticus2005

    • Author(s)
      L.Y. Zhou, T. Kobayashi(他6人)
    • Journal Title

      J. Mol. Endocrinol 35(1)

      Pages: 103-116

    • Peer Reviewed
  • [Journal Article] Molecular cloning, gene expression and characterization of the third estrogen receptor of the Nile tilapia, Oreochromis niloticus2005

    • Author(s)
      D.S. Wang, T. Kobayashi(他6人)
    • Journal Title

      Fish Physiol. Biochem 31(2, 3)

      Pages: 255-266

    • Peer Reviewed
  • [Journal Article] Ontogenic expression patterns of several nuclear receptors and cytochrome P450 aromatases in Brain and gonads of the Nile tilapia Oreochromis niloticus suggests their involvement in sex differentiation2005

    • Author(s)
      C.C. Sudhakumari, T. Kobayashi(他5人)
    • Journal Title

      Fish Physiol. Biochem 31(2,3)

      Pages: 129-135

    • Peer Reviewed
  • [Journal Article] Immunocytochemical localization of gonadotropins during the development of XX and XY Nile tilapia2005

    • Author(s)
      F. Sakai, T. Kobayashi(他7人)
    • Journal Title

      Fish Physiol. Biochem 31(2, 3)

      Pages: 177-181

    • Peer Reviewed
  • [Presentation] サンゴ礁に生息する魚類の性転換2009

    • Author(s)
      中村將
    • Organizer
      国際シンポジウム・海生動物の繁殖
    • Place of Presentation
      沖縄
    • Year and Date
      2009-02-21
  • [Presentation] 魚類の性決定・性分化とステロイドホルモン2008

    • Author(s)
      長濱嘉孝, 中村將
    • Organizer
      日本動物学会第79大会
    • Place of Presentation
      福岡大学
    • Year and Date
      20080905-07
  • [Presentation] サンゴ礁魚類の性転換 生殖生物学の歴史と展望2008

    • Author(s)
      中村將
    • Organizer
      基礎生物学研究所研究会
    • Place of Presentation
      岡崎コンファレンスセンター
    • Year and Date
      2008-03-24
  • [Presentation]2007

    • Organizer
      部会
    • Place of Presentation
      東京海洋大学
    • Year and Date
      20070327-31
  • [Presentation] 沖縄の魚の性転換2007

    • Author(s)
      中村將
    • Organizer
      第17回日本病態生理学会大会
    • Place of Presentation
      那覇市沖縄
    • Year and Date
      20070127-28
  • [Presentation] Endocrine Mechanism of Sex change in grouper. Korea-Japan2006

    • Author(s)
      M. Nakamura
    • Organizer
      Japan-Korea Joint Meeting on Reproductive Biology of Aquatic Animals.Jeju,Jeju Special Self-Governing Province
    • Place of Presentation
      Korea
    • Year and Date
      20061120-21
  • [Presentation] Sex change of grouer2006

    • Author(s)
      M. Nakamura
    • Organizer
      International Conference of Ecophysiology in Marine Orgarisms(海洋生物生理生態)
    • Place of Presentation
      国立台湾海洋大学
    • Year and Date
      20061003-04
  • [Presentation] Sex change in fish Marine Bio-Manipulation Frontier for Food Production The 4^<th> International Symposium(Joint International Symposia of the 21^<st> COE programs of Hokkaido University, Kinki University, Ehime University and University of the Ryukyus)2005

    • Author(s)
      M. Nakamura
    • Organizer
      Reproductive, Genetic and Disease Management in Aquaculture and Ocean Ranching
    • Place of Presentation
      Hakodate Kokusai Hotel
    • Year and Date
      20051008-11
  • [Presentation] Sex change in fish Marine Bio-Manipulation Frontier for Food Production The 4^<th> International Symposium(Joint International Symposia of the 21^<st> COE Proceedings of Hokkaido University, Kinki University, Ehime University and University of the Ryukyus)2005

    • Author(s)
      M. Nakamura
    • Organizer
      Reproductive, Genetic and Disease Management in Aquaculture and Ocean Ranching
    • Place of Presentation
      Hakodate Kokusai Hotel
    • Year and Date
      20051008-11
  • [Presentation] 魚類の性転換2005

    • Author(s)
      中村將
    • Organizer
      第76回大会日本動物学会
    • Place of Presentation
      つくば国際会議場
    • Year and Date
      20051006-08
  • [Book] 環境ホルモン-水産生物に対する影響実態と作用機構2006

    • Author(s)
      平井俊朗
    • Total Pages
      20
    • Publisher
      恒星社厚生閣、魚類の性分化と内分泌かく乱物質
  • [Book] 美ら島の自然史サンゴ礁島嶼系の生物多様性2006

    • Author(s)
      小林靖尚
    • Total Pages
      16
    • Publisher
      東海大学出版会サンゴ礁魚類の性の多様性-雌から雄へ、雄から雌へ-
  • [Book] 月周産卵魚カンモンハタの産卵関連行動「テレメトリー-水生動物の行動と漁具の運動解析」2006

    • Author(s)
      征矢野清
    • Total Pages
      9
    • Publisher
      恒星社厚生閣

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Published: 2010-06-10   Modified: 2016-04-21  

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