• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2008 Fiscal Year Final Research Report

Molecular mechanism of functions of photosynthetic CO_2-fixing enzyme RuBisCO

Research Project

  • PDF
Project/Area Number 17208031
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied molecular and cellular biology
Research InstitutionNara Institute of Science and Technology

Principal Investigator

YOKOTA Akiho  Nara Institute of Science and Technology, バイオサイエンス研究科, 教授 (40118005)

Co-Investigator(Kenkyū-buntansha) AKASHI Kinya  奈良先端科学気出大学院大学, バイオサイエンス研究科, 助教 (20314544)
ASHIDA Hiroki  奈良先端科学気出大学院大学, バイオサイエンス研究科, 助教 (50362851)
MUNEKAGE Yuri  奈良先端科学気出大学院大学, バイオサイエンス研究科, 助教 (30423247)
Project Period (FY) 2005 – 2008
Keywords光合成 / 炭素固定 / ルビスコ / 機能分子進化 / 機能改良
Research Abstract

ルビスコの反応触媒の分子機構およびルビスコ生合成の制御機構の解明を行ってきた.ルビスコ反応触媒の分子機構の解明を目指し、すでに枯草菌に発見しているルビスコ活性を持たないルビスコ祖先蛋白質とルビスコの反応中心残基の触媒反応への関わり方を明らかにした.我々が発見したもっとも高いSrel値を持つ紅藻Galdieriaルビスコと植物ルビスコの蛋白質構造比較から、オキシゲナーゼ反応抑制残基と想定している残基をラン藻ルビスコへ導入し、その機能を明らかにした.ルビスコ生合成の制御機構に関しては、ルビスコを正常に合成できない変異株を多数スクリーニングし、分子遺伝学手法によってそれらの変異原因遺伝子を特定し、その機能解析からルビスコ生合成の制御機構を明らかにした.

  • Research Products

    (33 results)

All 2009 2008 2007 2006

All Journal Article (11 results) (of which Peer Reviewed: 10 results) Presentation (19 results) Book (2 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] pfkB-type carbohydrate kinase family protein, NARA5, for massive expressions of plastid-encoded photosynthetic genes in Arabidopsis thaliana2009

    • Author(s)
      Taro Ogawa, Kenji Nishimura, Takehiko Aoki, Hisabumi Takase, Ken-Ichi Tomizawa, Hiroki Ashida and Akiho Yokota
    • Journal Title

      Plant Physiol. [Epub ahead of print]

    • Peer Reviewed
  • [Journal Article] Structure of the apo decarbamylated form of 2, 3-diketo-5-methylthiopentyl-1-phosphate enolase from Bacillus subtilis2009

    • Author(s)
      Haruka Tamura, Yohtaro Saito, Hiroki Ashida, Yasushi, Kai, Tsuyoshi Inoue, Akiho Yokota and Hiroyoshi Matsumura
    • Journal Title

      Acta Crystallograph Sec.D.65

      Pages: 942-951

    • Peer Reviewed
  • [Journal Article] The long-term responses of the photosynthetic proton circuit to drought2009

    • Author(s)
      Kaori Kohzuma, Jeff A. Cruz, Kinya Akashi, Saki Hoshiyasu, Yuri Nakajima-Munekage, Akiho Yokota and David M. Kramer
    • Journal Title

      Plant Cell Environm 32

      Pages: 209-219

    • Peer Reviewed
  • [Journal Article] Structural and functional similarities between a RuBisCO-like protein from Bacillus subtilis and photosynthetic RuBisCO.2009

    • Author(s)
      Yohtaro Saito, Hiroki Ashida, Tomoko Sakiyama, Nicole Tandeau de Marsac, Antoine Danchin, Agnieszka Sekowska, and Akiho Yokota
    • Journal Title

      J. Biol. Chem. 284

      Pages: 13256-13264

    • Peer Reviewed
  • [Journal Article] Crystallization and preliminary X-ray analysis of 2, 3-diketo-5-methylthiopentyl-1-phosphate enolase from Bacillus subtilis2009

    • Author(s)
      Haruka Tamura, Hiroki Ashida, Shogo Koga, Yohtaro Saito, Tomonori Yadani, Yasushi Kai, Tsuyoshi Inoue, Akiho Yokota and Hiroyoshi Matsumura
    • Journal Title

      Acta Crystallographica Section 65

      Pages: 147-50

    • Peer Reviewed
  • [Journal Article] RuBisCO-like proteins as the enolase enzyme in the methionine salvage pathway : Functional and evolutionary relationships between RuBisCO-like proteins and photosynthetic RuBisCO.2008

    • Author(s)
      Hiroki Ashida, Yohtaro Saito, Toshihiro Nakano, Nicole Tandeau de Marsac, Agnieszka Sekowska, Antoine Danchin and Akiho Yokota
    • Journal Title

      J. Exp. Bot. 59

      Pages: 1543-54

    • Peer Reviewed
  • [Journal Article] Molecular mechanisms of RuBisCO biosynthesis in higher plants2008

    • Author(s)
      Kenji Nishimura, Taro Ogawa, Hiroki Ashida, and Akiho Yokota
    • Journal Title

      Plant Biotech 25

      Pages: 285-290

  • [Journal Article] Enzymatic characterization of 5-methylthioribulose-1-phosphate dehydratase of the methionine salvage pathway from Bacillus subtilis.2008

    • Author(s)
      Hiroki Ashida, Yohtaro Saito, Chojiro Kojima and Akiho Yokota
    • Journal Title

      Biosci Biotechnol Biochem 72

      Pages: 959-967

    • Peer Reviewed
  • [Journal Article] Crystal structure of 5-methylthioribose 1-phosphate isomerase product complex from Bacillus subtilis : Implications for catalytic mechanism.2007

    • Author(s)
      Haruka Tamura, Yohtaro Saito, Hiroki Ashida, Tsuyoshi Inoue, Yasushi Kai, Akiho Yokota, and Hiroyoshi Matsumura
    • Journal Title

      Protein Sci. 17

      Pages: 126-35

    • Peer Reviewed
  • [Journal Article] Enzymatic Characterization of 5-Methylthioribose 1-Phosphate Isomerase from Bacillus subtilis2007

    • Author(s)
      Yohtaro Saito, Hiroki Ashida, Chojiro Kojima, Haruka Tamura, Hiroyoshi Matsumura, Yasushi Kai, Akiho Yokota
    • Journal Title

      Biosci Biotechnol Biochem 71

      Pages: 2021-8

    • Peer Reviewed
  • [Journal Article] Isabelle Callebaut, Agnieszka Sekowska, Elke Dittmann, Christiane Bouchier, and Nicole Tandeau de Marsac A New Rubisco-like Protein Coexists with a Photosynthetic Rubisco in the Planktonic Cyanobacteria Microcystis.2006

    • Author(s)
      Alyssa Carre-Mlouka, Annick Mejean, Philippe Quillardet, Hiroki Ashida, Yohtaro Saito, Akiho Yokota
    • Journal Title

      J. Biol. Chem. 281

      Pages: 24462-24471

    • Peer Reviewed
  • [Presentation] Improvement of Cyanobacterial Ribulose 1, 5-Bisphosphate Carboxylase/Oxygenase by Introducing the Latch Structure Involved in CO2/O2 Specificity in red algal RuBisCO2008

    • Author(s)
      Yohtaro Saito, Nana Ninomiya, Hiroki Ashida, and Akiho Yokota
    • Organizer
      The 4^<th> INDONESIAN BIOTECHNOLOGY CONFERENCE
    • Place of Presentation
      Nara Institute of Science and Technology (NAIST), Graduate School of Biological Sciences, 8916-5 Takayama, Ikoma, Nara 630-0101, Japan
    • Year and Date
      20080805-07
  • [Presentation] Analysis of common conserved residues between Bacillus RuBisCO-like protein and photosynthetic RuBisCO2008

    • Author(s)
      Toshihiro Nakano, Yohtaro Saito, Hiroki Ashida and Akiho Yokota
    • Organizer
      The 4^<th> INDONESIAN BIOTECHNOLOGY CONFERENCE
    • Place of Presentation
      Nara Institute of Science and Technology (NAIST), Graduate School of Biological Sciences, 8916-5 Takayama, Ikoma, Nara 630-0101, Japan
    • Year and Date
      20080805-07
  • [Presentation] メチオニン再生経路の酵素群によるリボース新規代謝経路2008

    • Author(s)
      中野寿宏、蘆田弘樹、齋藤洋太郎、横田明穂
    • Organizer
      日本農芸化学会2008 年度大会
    • Place of Presentation
      名城大学天白キャンパス
    • Year and Date
      2008-03-27
  • [Presentation] RuBisCO-like proteinの解析から見えてきたRuBisCO誕生の分子機構2008

    • Author(s)
      蘆田弘樹、横田明穗
    • Organizer
      日本植物生理学会第49回年会シンポジウム植物の生産性とカルビン回路「カルビンサイクル研究の新展開」
    • Place of Presentation
      ゲストスピーカー
    • Year and Date
      2008-03-22
  • [Presentation] 枯草菌RuBisCO-like proteinと光合成RuBisCOに共通する構造と機能2008

    • Author(s)
      齋藤洋太郎、蘆田弘樹、Agnieszka Sekowska、Antoine Danchin、横田明穗
    • Organizer
      第49回日本植物生理学会年会(奈良先端大・バイオ, パスツール研)
    • Place of Presentation
      札幌
    • Year and Date
      2008-03-20
  • [Presentation] (阪大院工、奈良先端大バイオ、福井工大)メチオニン回収経路で機能する枯草菌由来RLP のX 線結晶構造解析2007

    • Author(s)
      田村はるか、蘆田弘樹、斉藤洋太郎、井上豪、横田明穂、甲斐泰、松村浩由
    • Organizer
      日本結晶学会2007年度年会および総会
    • Place of Presentation
      東京
    • Year and Date
      20071201-02
  • [Presentation] (阪大院工・奈良先端大院分子生物学専攻・東北大院農)イネ由来RuBisCOと紅藻由来RuBisCOの硫酸イオン複合体に見られた立体構造の違い2007

    • Author(s)
      小村泰浩、松村浩由、蘆田弘樹、石田宏幸、上野剛、溝端栄一、井上豪、牧野周、横田明穂、前忠彦、甲斐泰
    • Organizer
      日本結晶学会2007年度年会および総会
    • Place of Presentation
      東京
    • Year and Date
      20071201-02
  • [Presentation] (Department of Applied Chemistry, Osaka University ; Department of Molecular biology, Nara Institute of Science and Technology (NAIST) ; CREST (Sosho Project), JST)Crystal structure of 5-methylthioribose 1-phosphate isomerase product complex from Bacillus subtilis : Implications for catalytic mechanism2007

    • Author(s)
      Haruka Tamura, Yohtaro Saito, Hiroki Ashida, Tsuyoshi Inoue, Yasushi Kai, Akiho Yokota, and Hiroyoshi Matsumura
    • Organizer
      第8回アジア結晶学会連合会議
    • Place of Presentation
      台北
    • Year and Date
      20071104-07
  • [Presentation] (Department of Applied Chemistry, Osaka University ; Department of Molecular biology, Nara Institute of Science and Technology (NAIST) ; Department of Applied Plant Science ; Tohoku University, CREST (Sosho Project), JST)STRUCTURAL DIFFERENCE BETWEEN RICE AND RED ALGA RUBISCO COMPLEXED WITH SULFATE2007

    • Author(s)
      Yasuhiro Komura, Hiroyoshi Matsumura, Hiroyuki Ishida, Hiroki Ashida, EiichiMizohata, Tsuyoshi Inoue, Amane Makino, Tadahiko Mae, Akiho Yokota, Yasushi Kai
    • Organizer
      第8回アジア結晶学会連合会議(The 8th Conference of the Asian Crystallographic Association, AsCA'07)
    • Place of Presentation
      台北
    • Year and Date
      20071104-07
  • [Presentation] Evolutionary potential of RuBisCO-like protein in Bacillus subtilis : Interaction with transition-state analogue of RuBisCO2007

    • Author(s)
      Yohtaro SAITO, Hiroki ASHIDA, Agnieszka SEKOWSKA, DANCHIN, and Akiho YOKOTA
    • Organizer
      14^<th> International congress of photosynthesis(poster)
    • Place of Presentation
      Glasgow, UK
    • Year and Date
      20070722-27
  • [Presentation] Improvement of cyanobacterial RuBisCO by introducing the latch structure involved in high affinity for CO_2 in red algal RuBisCO2007

    • Author(s)
      Nana NINOMIYA, Hiroki ASHIDA, andAkiho YOKOTA
    • Organizer
      14^<th> International congress of photosynthesis(poster)
    • Place of Presentation
      Glasgow, UK
    • Year and Date
      20070722-27
  • [Presentation] RuBisCO-like proteins, Research Frontiers with Rubisco - the "Elixir of Life" in the Biosphere.2007

    • Author(s)
      Hiroki ASHIDA and Akiho YOKOTA
    • Organizer
      14^<th> International Congress of Photosynthesis satellite Meeting, (guest speaker)
    • Place of Presentation
      Rothamsted Research, Harpenden, Herts, UK
    • Year and Date
      20070720-21
  • [Presentation] 高CO_2親和性を示す紅藻RuBisCOが有するラッチ構造のラン藻RuBisCOへの導入2007

    • Author(s)
      二宮奈々、蘆田弘樹、横田明穗
    • Organizer
      2007年度松山年会(奈良先端大・バイオ)
    • Place of Presentation
      愛媛大学城北キャンパス
    • Year and Date
      2007-03-28
  • [Presentation] 枯草菌RuBisCO-like protein とRuBisCOの相互構造活性相関2007

    • Author(s)
      中野寿宏、齋藤洋太郎、蘆田弘樹、横田明穂
    • Organizer
      日本農芸化学会2007年度大会
    • Place of Presentation
      東京農業大学世田谷キャンパス
    • Year and Date
      2007-03-26
  • [Presentation] 光合成カルビン回路完成の分子的基盤2006

    • Author(s)
      蘆田弘樹、横田明穗
    • Organizer
      日本光合成研究会第6回ワークショップセミナーNew perspective of photosynthesis research光合成研究の新たな潮流構造とゲノムそして未来(奈良先端大、バイオサイエンス)
    • Place of Presentation
      大阪大学ゲストスピーカー
    • Year and Date
      20061012-13
  • [Presentation] ゲノムから見たカルビン回路完成の分子機構2006

    • Author(s)
      蘆田弘樹、横田明穂
    • Organizer
      日本植物学会第70回大会、シンポジウムゲノムから見た光合成機能の再発見(奈良先端大、バイオサイエンス)
    • Place of Presentation
      熊本ゲストスピーカー
    • Year and Date
      20060913-16
  • [Presentation] X-ray structure of methylthioribose-1-phosphate isomerase from Bacillus subtilis reveals a hydride transfer mechanism of catalysis and a structure function relationship with other homologues2006

    • Author(s)
      Haruka TAMURA, Hiroyoshi Matsumura, Yohtaro SAITO, Tsuyoshi INOUE, HirokiASHIDA, Akiho YOKOTA, and Yasushi KAI
    • Organizer
      20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress
    • Place of Presentation
      Kyoto, Japan
    • Year and Date
      2006-06-22
  • [Presentation] Structure-function relationship between RuBisCO-like protein of Bacillus subtilis and photosynthetic RuBisCO2006

    • Author(s)
      Yohtaro SAITO, Agnieszka SEKOWSKA, Hiroki ASHIDA, Antoine DANCHIN, andAkiho YOKOTA
    • Organizer
      20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress
    • Place of Presentation
      Kyoto, Japan
    • Year and Date
      2006-05-19
  • [Presentation] Nicole Tandeau de Marsac and Akiho Yokota, Molecular evolution of RuBisCO-like proteins2006

    • Author(s)
      Hiroki Ashida, Tomoko Sakiyama, YohtaroSaito
    • Organizer
      20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress((poster))
    • Place of Presentation
      Kyoto, Japan
    • Year and Date
      2006-05-19
  • [Book] 光、栄養環境強光乾燥ストレスに対する植物の多次元的応答 蛋白質核酸酵素増刊(Vol.52, No.6)2007

    • Author(s)
      明石欣也、宗景ゆり、高原健太郎、横田明穂
    • Total Pages
      594-600
    • Publisher
      植物における環境とあ生物ストレスに対する応答
  • [Book] 第2章エネルギーと代謝第4節光合成CO_2固定酵素RuBisCO2006

    • Author(s)
      蘆田弘樹、横田明穗
    • Total Pages
      240-247
    • Publisher
      プラントミメティックス~植物に学ぶ~
  • [Patent(Industrial Property Rights)] 変異型のリブロース-1, 5-ビスリン酸カルボキシラーゼ/オキシゲナーゼおよびその利用2007

    • Inventor(s)
      蘆田弘樹、横田明穂
    • Industrial Property Rights Holder
      安田國雄
    • Industrial Property Number
      2007-028608
    • Filing Date
      2007-02-07

URL: 

Published: 2010-06-10   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi