2007 Fiscal Year Final Research Report Summary
Development of regeneration, purification, and transplantation of cardiomyocytes from mouse and common marmoset ES cells
| Project/Area Number |
17209029
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Circulatory organs internal medicine
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| Research Institution | Keio University |
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Principal Investigator |
FUKUDA Keiichi Keio University, School of Medicine, Professor (20199227)
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| Co-Investigator(Kenkyū-buntansha) |
MURATA Mitsushige Keio University, School of Medicine, Instructor (30317135)
YUASA Shinsuke Keio University, School of Medicine, Instructor (90398628)
HATTORI Fumiyuki Keio University, School of Medicine, Researcher (50398624)
TANIOKA Yoshikuni Central Institute for Experimental Animals, Animal Experimentation Center, Researcher(non-tenuerd) (10072406)
SASAKI Erika Central Institute for Experimental Animals, Animal Experimentation Center, Researcher(non-tenuerd) (70390739)
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| Project Period (FY) |
2005 – 2007
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| Keywords | human embryonic stem cell / common marmosset / Regeneratie Medicine / Cardiomyocyte / differentiation / Development / Noggin / Cell Transplantation |
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| Research Abstract |
This Study tried to establish a novel method for generation, purification, and transplantation of new cardiomyocytes from human and common marmoset ES cells. We found that Noggin induce differentiation from ES cells to antero-lateral mesodermal cell, and that factor X promote differentiation from antero-lateral mesodermal cells to cardiomyocytes. Moreover, we found that Y-receptor is strongly expressed at the early differentiated cardiomyocytes, and administration of Y ligand promoted cell division in embryonic cardiomyocyte in vivo and early differentiated cardiomyocytes from ES cells. In combination with these factors, we can produce cardiomyocytes from marmoset and human ES cells. We also developed a novel method to purify cardiomyocytes from the mixture of cells by using mitochondria-specific incorporated dye. We transplanted the cardiomyocytes purified by this method, and the grafted cardiomyocytes can eliminate teratoma formation. Moreover, we also developed a novel method to enhance the cell survival after transplantation by a re-aggregation method. The grafted cell survival by needle injection into the heart was less than 1%, while this method can improve the efficiency up to 90%. We transplanted the purified cardiomyocytes into immuno-deficient mouse and common marmoset monkeys, In combination with these various techniques, we developed an efficient method to purify cardiomyocytes.
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Research Products
(11 results)
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[Journal Article] Common marmoset embryonic stem cell can differentiate into Cardiomyocytes.2008
Author(s)
Hao Chen, Fumiyuki Hattori, Mitsushige Murata, Weizhen Li, Shinsuke Yuasa, Takeshi Onizuka, Kenichiro Shimoji, Yohei Ohno, Erika Sasaki, Kensuke Kimura, Daihiko Hakuno, Motoaki Sano, Shinji Makino, Satoshi Ogawa, Keiichi Fukuda.
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Journal Title
Biophys Biochem Res Comm. 369
Pages: 801-806
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Chondromodulin-I maintains cardiac valvular function by preventing angiogenesis.2006
Author(s)
Masatoyo Yoshioka, Shinsuke Yuasa, Kensuke Kimura, Naritaka Kimura, Keisuke Matsumura, Takayuki Shiomi, Chisa Shukunami, Yasunori Okada, Makio Mukai, Hankei Shin, Ryohei Yozu, Masataka Sata, Satoshi Ogawa, Yuji Hiraki, Keiichi Fukuda.
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Journal Title
Nat Med 12
Pages: 1151-1159
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Transient and strong inhibition of BMP signals by Noggin induces cardiomyocyte differentiation in murine embryonic stem cells.2005
Author(s)
Shinsuke Yuasa, Yuji Itabashi, Uichi Koshimizu, Tomofumi Tanaka, Keijiro Sugimura, Masayoshi Kinoshita, Fumiyuki Hattori, Shin-ichi Fukami, Takuya Shimazaki, Satoshi Ogawa, Hideyuki Okano, Keiichi Fukuda.
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Journal Title
Nature Biotechnology 23
Pages: 607-611
Description
「研究成果報告書概要(欧文)」より
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