2007 Fiscal Year Final Research Report Summary
Investigation of molecular mechanisms of Sox9 transcriptional factory during enchandral ossification
| Project/Area Number |
17209059
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional basic dentistry
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| Research Institution | Osaka University |
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Principal Investigator |
YONEDA Toshiyuki Osaka University, Graduate School of Dentistry, Professor (80142313)
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| Co-Investigator(Kenkyū-buntansha) |
NISHIMURA Rikou Osaka University, Graduate School of Dentistry, Associate Professor (60294112)
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| Project Period (FY) |
2005 – 2007
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| Keywords | chondrocyte / transcriptional factory / Sox9 |
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| Research Abstract |
Tb investigate the molecular mechanism by which Sox9 plays an essential role in chondrocyte differentiation, we established a gene screening system for identifying the members of Sox9 transcriptional factory. Using this system, we could identify a calcium ion channel, TRPV_4, as a member of Sox9 transcriptional factory, and found that TRPV4 upregulated expression and function of Sox9, thereby stimulating chondrocyte differentiation. We further screened cDNA library generated from chondrogenic cell line, ATDC5, and isolated the Znf219 cDNA. Whole mount in situ and RT-PCR analyses indicated that Znf219 is specifically expressed in limb bud of E12.5 embryo mice and chondrocytes. Co-immunoprecipitation experiments indicated that Znf219 physically interacts with Sox9. Moreover, we found that Znf219 co-localized with Sox9 in the nucleus. These results indicated that Znf219 forms transcriptional factory in the nucleus. To understand the functional role of Znf219 m chondrocyte differentiation, we next examined the effect of overexpression of Zof219 on chondrocyte differentiation. We found that overexpression of Znf219 stimulated the chondrogenic activity of Sox9. In contrast, overexpression of a dominant negative Znf219 markedly inhibited chondrocyte differentiation induced by Sox9. Furthermore, knockdown of Znf219 using micro RNA system suppressed chondrocyte differentiation. Collectively, our results demonstrated that Znf219 might play an important role in Sox9-regulated chondrocyte differentiation.
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Research Products
(30 results)
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[Journal Article] Phenotypic analysis of Dlx5 overexpression in post-natal bone2008
Author(s)
Zhang, J, Zhu, J, Valverde, P, Li, L, Pageau, S, Tu, Q, Nishimura, R, Yoneda, T, Yang, P, Zheng, W, Ma, W, Chen, J
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Journal Title
J Dent Res 87
Pages: 45-50
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Differential expression of matrix metalloproteinases in brain- and bone-seeking clones of metastatic MDA-MB-231 breast cancer cells2007
Author(s)
Stark, AM, Palmieri, D, Meter, PS, Walker, RT, Mentlein, R, Yoneda, T, Mehdorn, HM, Steeg, PS, Held-Feindt, J
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Journal Title
J Neurooncol 81
Pages: 39-48
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Her-2 overexpression increases the metastatic outgrowth of breast cancer cells in the brain2007
Author(s)
Palmieri, D, Bronder, JL, Herring, JM, Yoneda, T, Weil, RJ, Stark, AM, Kurek, R, Vega-Valle, E, Feigenbaum, L, Halverson, D, Vortmeyer, AO, Steinberg, SM, Aldape, K, Steeg, PS
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Journal Title
Cancer Res 67
Pages: 4190-4198
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] NAD(P)H oxidases regulate HIF-2a protein expression2007
Author(s)
Block, K, Gorin, Y, Hoover, P, Williams, P, Chelmicki, T, Clark, RA, Yoneda, T, Abboud, HE
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Journal Title
J Biol Chem 282
Pages: 8019-8026
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] lhh/Gli2 signaling promotes osteoblast differentiation by regulating Runx2 expression and function2007
Author(s)
Shimoyama, A, Wada, M, Ikeda, F, Hata, K, Matsubara, T, Nifuji, A, Noda, M, Amano, K, Yamaguchi, A, Nishimura, R, Yoneda, T
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Journal Title
Mol Biol Cell 18
Pages: 2411-2418
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Functional gene screening system identified TRPV4 as a regulator of chondrogenic differentiation2007
Author(s)
Muramatsu, S, Wakabayashi, M, Ohno, T, Ooishi, R, Sugahara, T, Shiojiri, S, Suzuki, Y, Sugano, S, Nishimura, R, Yoneda, T, Matsuda, A
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Journal Title
J Biol Chem 282
Pages: 32158-32167
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Activation of NFAT signal in vivo leads to osteopenia associated with increased osteoclastogenesis and bone resorbing activity2006
Author(s)
Ikeda, F, Nishimura, R, Matsubara, T, Hata, K, Reddy, SV, Yoneda, T
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Journal Title
J Immunol 177
Pages: 2384-2390
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Critical role of cortactin in actin ring formation and osteoclastic bone resorption2006
Author(s)
Matsubara, T, Myoui, A, Ikeda, F, Hata, K, Yoshikawa, H, Nishimura, R, Yoneda, T
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Journal Title
J Bone Miner Metab 24
Pages: 368-372
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Osteoclasts play a part in pain due to the inflammation adjacent to bone2006
Author(s)
Nagae, M, Hiraga, T, Wakabayashi, H, Wang, L, lwata, K, Yoneda, T
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Journal Title
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Ac-Fms tyrosine kinase inhibitor, Ki20227, suppresses osteoclast differentiation and osteolytic bone destruction in a bone metastasis model2006
Author(s)
Ohno, H, Kubo, K, Murooka, H, Kobayashi, Y, Nishitoba, T, Shibuya, M, Yoneda, T, lsoe, T
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Journal Title
Mol Cancer Ther 5
Pages: 2634-2643
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Co-localization of cortactin and phosphotyrosine identifies active invadopodia in human breast cancer cells2006
Author(s)
Bowden, ET, Onikoyi, E, Slack, R, Myoui, A, Yoneda, T, Yamada, KM, Mueller, SC
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Journal Title
Exp Cell Res 312
Pages: 1240-1253
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] In vivo MRI of cancer cell fate at the single cell level in a mouse model of breast cancer metastasis to the brain2006
Author(s)
Heyn, C, Ronald, JA, Ramadan, SS, Snir, JA, Barry, AM, MacKenzie, LT, Mikulis, DJ, Palmieri, D, Bronder, JL, Steeg, PS, Yoneda, T, MacDonald, IC, Chambers, AF, Rutt, BK, Foster, PJ
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Journal Title
Magnetic Resonance Med 56
Pages: 1001-1010
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] CCAAT/enhancer binding protein β isoform, liver-enriched inhibitory protein, regulates commitment of osteoblasts and adipocytes2005
Author(s)
Hata, K, Nishimura, R, Ueda, M, Ikeda, F, Matsubara, T, lchide, F, Hisada, K, Nokubi, T, Yamaguchi, A, Yoneda, T
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Journal Title
Mol Cell Biol 25
Pages: 1971-1979
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Preferential inhibition of bone metastases by 5'-deoxy-5-fluorouridine and capecitabine in the 4T1/luc mouse breast cancer model2005
Author(s)
Hiraga, T, Hata, K, Ikeda, F, Kitagaki, J, Fujimoto-Ouchi, K, Tanaka, Y, Yoneda, T
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Journal Title
Oncol Report 14
Pages: 695-699
Description
「研究成果報告書概要(欧文)」より
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